Official publication of TIFAC CORE in Green

Pharmacy
Home About us Instructions Submission Subscribe Advertise Contact e-Alerts Login

Users Online: 10
ORIGINAL ARTICLE
Year : 2009 | Volume : 3 | Issue : 1 | Page : 47-51
Table of Contents
Study of extraction and HPTLC - UV method for estimation of caffeine in marketed Similar in PUBMED
tea ( Camellia sinensis ) granules Search Pubmed for

H Misra1, D Mehta2, BK Mehta2, M Soni3, DC Jain1 - Misra H

1
School of Studies in Chemistry and Biochemistry, Vikram University, Ujjain - 456 - Mehta D
010; Green Technology Department, Ipca Laboratories Limited, Ratlam - 457 002, - Mehta BK
India - Soni M
2
School of Studies in Chemistry and Biochemistry, Vikram University, Ujjain - 456 - Jain DC
010, India
3
Mandsaur Institute of Pharmacy, Mandsaur - 458 001, Madhya Pradesh, India Citation Manager
Access Statistics
Correspondence Address:
D C Jain Reader Comments
Green Technology Department, Ipca Laboratories Limited, Ratlam - 457 002, Email Alert *
Madhya Pradesh Add to My List *
India * Requires registration (Free)

Source of Support: None, Conflict of Interest: None

Article Access Statistics
DOI: 10.4103/0973-8258.49374 Viewed 56
Printed 0
A simple, precise and accurate high-performance thin-layer chromatographic Emailed 0
(HPTLC) method has been established and validated for screening and quantitative
estimation of caffeine in different extracts of tea samples ( Camellia sinenesis ) . PDF Downloaded 26
Separation was performed on silica gel 60 F 254 HPTLC plates with ethyl Comments [Add]
acetate:methanol in the proportion of 27: 3 ( v/v ), as a mobile phase. The
determination was carried out in the ultraviolet (UV) region using the densitometric
remission-absorbance mode at 274nm. Maximum recovery of caffeine was achieved
when extracted with 5% diethyl amine in DM water (v/v). The maximum
concentration of caffeine in tea samples was found to be 2.145%, dry weight basis.
Caffeine response was found to be linear over the range of 2-14µg per zone. Limits
of detection and quantitation were found to be 40 and 120ng/spot, respectively. The
HPTLC method was validated in terms of precision, accuracy, sensitivity and
robustness. Some rare parameters for the HPTLC method like calculation of flow
constant (k) and plate efficiency (N) are included specially.
Gadgets powered by Google

[FULL TEXT [PDF]*

About us | Contact us | Sitemap | Advertise | What's New | Feedback | Copyright and Disclaimer
© 2009 International Journal of Green Pharmacy | A journal by Medknow
Online since 15th February, 2008
www.brncop.org | www.asiapharmaceutics.info | www.aiip.info</A< td>

ISSN: Print -0973-8258, Online - 1998-4103