Any questions about LIGHTrun?

customerservice@gatc-biotech.com

peed Light-sn g seque cin e light pric

NEW

g service: sequencin order only barcode ncing order no seque nt ple shipme m a s e e fr
ered Data deliv 1pm from 8am next day!

Germany Tel. +49 7531 81 60 68 Great Britain Tel. +44 207 691 4921 France Tel. +33 97 04 46 743 Sweden Tel. +46 8 655 3609

HTrun Test LIG g service n ci en u q se free t es u eq R odes now! test barc

Jochen Schfer, Head of Single Read Sequencing Services, GATC Biotech

www.gatc-biotech.com/lightrun
WWW.GATC-BIOTECH.COM

www.gatc-biotech.com/lightrun

Sample requirements
Purified plasmid DNA or purified PCR fragments with primer premixed in tubes

Sample shipment
1. GATC Collection Box There are many GATC Collection Boxes installed throughout Europe for free sample shipment. Look for the one near you. 2. FREE shipment by UPS If there is no GATC Collection Box near you, we will provide you with UPS labels for shipment free of charge (depending on the size of the barcode batch ordered). 3. By post If there is no GATC Collection Box near you, and you only have a few samples to send, please ship them by post. Use stable, padded envelopes or falcon tubes to ensure safe transport. Please then send your samples to:

1. 2.

Template DNA of 5l with either of following concentrations: Plasmid DNA (purified): 80100 ng/l PCR product (purified): 20 80 ng/l Add 5 l of primer with following concentration: 5M (5pmol/l) Please send total sample amount of 10 l in 1,5 ml tubes.

Primer characteristics The melting temperature (TM) of the primer should be between 52 and 58C and the length should be between 1719bp. Ideally, the GC content of an 17mer should be 10 G+C; for an 18mer 89 G+C and for a 19mer 79 G+C. G or C should be at the 3 end, but not more than 3 Gs or Cs. The primer sequence should be a good mix of all 4 nucleotides with no more than 4 identical bases in a row (AAAA or GGGG).
To ensure the best possible quality of sequence data, the total volume of the sample cannot be less than 10l. We recommend measuring the DNA concentration on an agarose gel. Sequencing reactions cannot be repeated and all reactions have to be paid. Your DNA material cannot be stored. Thank you for your understanding.

GATC Biotech AG European Custom Sequencing Centre Gottfried-Hagen-Strae 20 51105 Kln

LIGHTrun in 5 easy steps:

1.
Order LIGHTrun sequencing barcodes Go to www.gatc-biotech. com/lightrun and click on Order LIGHTrun barcodes. Choose service type and order your LIGHTrun sequencing barcodes. They will be sent to you without delay.

2.
Premix template and primer Add to your tube: 5 l template + 5 l primer (according to sample requirements).

3.
Label tubes with barcodes Attach one part of the barcode to the tube and keep the other part for your lab record. Affix the barcode in the front of the tube Please dont use parafilm to seal your tubes!

4.
Send your samples Send by post, drop them at a GATC Collection Box or send by UPS (depending on order volume).
tRun Ligh ubes T for

5.
Download results Once your data are ready, youll receive an email. Go to www. gatc-biotech.com/lightrun and click on Download results.

LIGHTrun barcode types

LIGHTrun tubes
delivery time: 1day upon sample receipt delivery time: 8am1pm next day, after sample drop-off LightRun I lab record Seq. ID 12AA34 LightRun for Tubes

LIGHTrun service types

LIGHTrun 96 well plates
delivery time: 1day upon sample receipt
Seq. ID 12AA34

LIGHTrun NXP tubes

Single read sequencing of purified plasmids or PCR fragments

LightRun I lab record Seq. ID 12AA34

LightRun for Tubes

1.
LIGHTrun tubes up to 1,100nt delivery time: 1day

2.
LIGHTrun NXP tubes up to 1,100nt delivery time: 8am 1pm the next day

3.
LIGHTrun 96 well plates up to 1,000nt delivery time: 1day

This part for your lab record

This part for your lab record

This part for your lab record

This part for your tubes

This part for your tubes

This part for your plates

LightRun for Plate

Seq. ID 12AA34

Seq. ID 12AA34

LightRun I lab record Seq. ID 12AA34

For tubes: each LIGHTrun barcode label corresponds to one single sequencing reaction For 96 well plates: each LIGHTrun barcode label corresponds to one entire plate
Next day data delivery 8 am - 1 pm depends on logistics available at your location.