Beruflich Dokumente
Kultur Dokumente
Male, Married Indian Senior Principal Scientist, Biotechnology Division CSIR-Institute of Himalayan Bioresource Technology, Palampur-176 061 Distt., Kangra (HP), INDIA Tel: +91 1894 23 33 39 Ext 340 Mobile: 98166 2 1463 Email: sanjaykumar@ihbt.res.in, sanjayplp@rediffmail.com; sanjayplp1@gmail.com
6. Educational Qualifications
1986 M.Sc. (Plant Physiology; O.G.P.A.- 4.6/5.0 (84.0%) First Division; Thesis Title: Studies on Photosynthetic Enzymes and Carbon Flux in the Photosynthetic and Glycolate Pathways at Different Osmotic Potentials in Sorghum (Sorghum bicolor L. var. SPV-351); Supervisor: Dr. R.K. Gupta) G.B. Pant University of Agriculture and Technology, Pantnagar, India-263 145. 1991 Ph.D. (Plant Physiology with Biochemistry and Molecular Biology; O.G.P.A.-3.88/4.00; Thesis Title: Importance of Hydrogen Peroxide Metabolism and Cyanide Resistant Respiration in Water Stressed Plants and Thermogenesis; Supervisor: Professor S.K. Sinha, FNA, FASc., FNASc., Professor of Eminence) Indian Agricultural Research Institute, New Delhi, India- 110 012.
Sanjay Kumar
Sanjay Kumar
8. Awards/ recognitions
1. Young Scientist Award 1995 by Indian National Science Academy, New Delhi, India INSA Medal for Young Scientists) for work on "Thermogenesis in Plants- Implications in Stress, Ripening and Senescence". (http://insaindia.org/youngmedal.htm) 2. Certificate of Merit in CSIR Leadership Programme 2007(01) on March 6th, 2008 by the HRDG, CSIR 3. Plenary lecture on Understanding altered molecular dynamics in Himalayan ecosystem: implications for agricultural research under climate change scenario during International Conference on Preparing Agriculture for Climate Change organized by the Punjab Agriculture University, Ludhiana on 7th Feb., 2011. 4. Member The Scientific Advisory Committee of J&K State Council for Science & Technology, constituted vide Govt. order No. 46-ST of 2010 dated 17-6-2010. 5. Member working group on National Mission on Himalayan Eco-system, Department of Environment, Science and Technology, Govt. of Himachal Pradesh. EnV&ST(F), 08-09-2011. 6. Invited by the scientific organizing committee , XVIII International Botanical Congress-2011 to contribute to developing a proposal for the symposium on Plants in human health and to deliver a talk during XVIII International Botanical Congress held in Melbourne, Australia during July 2330th 2011. 7. Co-Chaired the technical session on Himalayan Biodiversity (on 21st Dec., 2010) during International Workshop on Biodiversity and Climate Change
Sanjay Kumar organized by Centre for Oceans, Rivers, Atmosphere and Land Sciences, Indian Institute of Technology, Kharagpur, India during 19th 22nd December 2010. 8. Served as Panelist (on 21st Dec., 2010) during International Workshop on Biodiversity and Climate Change organized by Centre for Oceans, Rivers, Atmosphere and Land Sciences, Indian Institute of Technology, Kharagpur, India during 19th 22nd December 2010. 9. International Research Associateship under Ecogen project, funded by Targetted Excellece at Kansas State University, Manhattan, Kansas, USA. This was a highly competitive International Research grant. Working with Dr. Loretta Johnson and Jyoti Shah. The project involves understanding the response of roots to nitrogen and water using microarray and subtractive hybridization approaches. 10. DBT Overseas Associateship, 2001 to carry out advanced research at Rothamsted Research, Harpenden, UK during Feb, 6 to Aug 5th, 2003. Worked with Prof Christine H. Foyer. 11. Long term BOYSCAST (Better Opportunities for Young Scientists in the Chosen Areas of Science and Technology) Fellowship by the Department of Science and Technology (DST), Government of India for the year 1992-1993 to carry out advanced research/training in the area of plant molecular biology at Texas Tech University, Lubbock, USA (March 21, 1994- March 20, 1995). Worked with Prof. Henry T. Nguyen 12. Senior and Junior Research Fellowship, IARI, New Delhi 13. Teaching Assistantship, G.B. Pant Univ. Agric and Tech, Pantnagar during M.Sc.
Sanjay Kumar
9. Employment
Period Designation Organisation & Place CSIR Complex Palampur (now CSIR-Institute of Himalayan Bioresource Technology, CSIR-IHBT) CSIR-IHBT, Palampur Mode of Appointment Rs. 2,200-75Open 2,800-100-4,000 Competition (Pre-prerevised) Pay Scale Nature of Duties
Research, project conceptualization and formulation and carrying out research on Himalayan plants
Scientist C [Gr.IV(II)]
Rs. 10,000-325- By Assessment 15,200 (Pre revised) Rs. 12,000-375- By Assessment 16,500 (Pre revised)
Research, project conceptualization and formulation and carrying out research on Himalayan plants Research, project conceptualization and formulation and carrying out research on Himalayan Bioresources; developing infrastructure and assisting director on various aspects Research, project conceptualization and formulation and carrying out research on Himalayan Bioresources; developing infrastructure and assisting director on various aspects Innovating and developing research programmes, setting up state-of-art labs for genomics, proteomics and metabolomics, human resource development through teaching, guiding PhD/MScs and imparting summer /winter trainings; fulfillment of administrative responsibilities and assisting directors on various fronts.
Jan, 2004- 30th Scientist EII CSIR-IHBT, April, 2008 [Gr.IV(IV)] Palampur
CSIR-IHBT, Palampur
Sanjay Kumar
Duration
March 21st , 1994- March 20th, 1995 Feb, 6th to Aug 5th, 2003 Mar, 6th, 2006 to Aug 14th 2006
Summary of work/publication
Worked on molecular aspects of temperature stress. Joshi, C.P., Kumar, S. and Nguyen, H.T. 1996. Application ------ gene family. Plant Molecular Biology, 30: 641-646. Worked on molecular aspects of ascorbate metabolism in cell death and disease resistance. Pavet, V., Olmos, E., Kiddle, G., Mowla, S., Kumar, S., ----Foyer, C.H. 2005. Ascorbic -thaliana. Plant Physiology 139: 1291-1303. Worked on ecological genomics
Institut fr Chemie und Dynamik der Geosphre, ICG-3, Phytosphre Forschungszentrum, Jlich GmbH, D-52425 Germany vide OM no.22(62)06-Estt. Dt. 3.4.2009 Melbourne, Australia
The 2nd MS Technology Seminar (Proteomics Program) to understand various aspects of proteomic technology developed by Waters. Invited by the ICG-3 to develop a collaborative research programme with IHBT, Palampur.
Institute of Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Jlich GmbH, D52425 Jlich Germany
Berlin and Institute of Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Jlich GmbH, D-52425 Jlich Germany
Invited by the XVIII International Botanical Congress 2011 to deliver a talk in symposium 159 entitled Plants in human health and well-being during XVIII International Botanical Congress 2011 (23-30 July, 2011). For advanced discussions on plant phenotyping, and to attend the annual meeting on our IndoGerman project funded by the Indo-German Science and Technology Centre (IGSTC) and DST/BMBF. To attend the meeting on Indo-German project funded by the Indo-German Science and Technology Centre (IGSTC) and advanced discussions on plant phenotyping.
Sanjay Kumar
Sanjay Kumar 11. Nov 8th, 2008 on Bioprospecting Genes and Pathways from Himalayan Flora: Implications to Agriculture during national symposium on New Biology in Agriculture organized by Panjab University, Chandigarh in Collaboration with All India Biotech Association, New Delhi at Panjab University, Chandigarh. 12. Feb 28, 2009 on Nanotechnology: Twists, Grips and Reaching out during Science Day organized at IHBT, Palampur. 13. March 9th, 2009 on Transgenic Research at IHBT during Workshop on "Management and Monitoring of Field Trails of Genetically Engineered Crops" sponsored by DBT, GOI organized at Y.S. Parmar University of Horticuture and Forestry, Solan, HP. 14. March 21st, 2009 Science and the Society for the schools children during annual function of the Parmarth International School, Baijnath. Attended the function on behalf of the Director. School honoured IHBT for its contribution to science and presented a memento to the Institute. 15. September 26th, 2009 CSIR foundation Lecture on Climate Change: National Issues and Himalayan Efforts at the Institute of Himalayan Bioresource Technology, Palampur. 16. February 23rd, 2010 Agri-instrumentation for Tea, Genomics and Environment at CSIO, Chandigarh at one day workshop during Golden Jubilee Celebration of CSIO 17. Sept, 28, 2010 Bioprospecting genes, enzymes and processes from Himalayan flora for plant adaptation and synthetic biology Central University of Punjab, Bathinda (Pb). Invited lecture to the staff and students of CUP. 18. December 21, 2010 Exploratory studies on climate change and adaptation of species complexes at technical session on Himalayan Biodiversity during International Workshop on Biodiversity and Climate Change organized by Centre for Oceans, Rivers, Atmosphere and Land Sciences, Indian Institute of Technology, Kharagpur, India during 19th 22nd December, 2010 19. February 7th, 2011 Plenary lecture on Understanding altered molecular dynamics in Himalayan ecosystem: implications for agricultural research under climate change scenario during Internation Conference on Preparaing Agriculture for Climate Change organized by the Punjab Agriculture University, Ludhianaon. 20. July 26th, 2011 Bioprospecting flavonoid and terpenoid pathways in target plants tea, picrorhiza and arnebia during XVIII International Botanical Congress 2011 (23-30 July, 2011) Melbourne, Australia in symposium 159 entitled Plants in human health and well-being.
Sanjay Kumar 21. October 13th, 2011 What have we learnt from altitude? during BIOTECHNIKA2011: National Symposium on Current Perspectives in Biotechnology & Talent Hunt under the aegis of UGC-SAP & NASI organized y the Panjab University, Chandigarh. 22. November 15th, 2011 Utilizing genomic and metabolic plasticity at varying altitude for plant improvement during 80th Annual Meeting of the SBC(I) on metabolic pathway modulations-applications in health and agriculture organized by CIMAP, Lucknow. 23. November 26th, 2011 Transcriptome analysis: understanding and utilizing the unctionality of genome during National Symposium on Genomics for sustainable food and nutritional security at CPRI, Shimla. 24. March 19th, 2012 Molecular basis of biosynthesis of flavonoids and terpenoids: case studies on Camellia sinensis and Picrorhiza kurrooa during National Seminar on "Current Trends in Secondary Plant Metabolite Research" (March 19 to March 20,2012) at Jamia Hamdard, New Delhi. 25. September 24th, 2012, Need, Nature: The Teacher- By Chance: The Leaper during INSPIRE organized by DST and CSIR-IHBT at CSIR-IHBT, Palampur.
Sanjay Kumar
1. Physiological and biochemical basis of leaf senescence in tea (Camellia sinensis L. (O.) Kuntze). 2. Isolation and characterization of dormancy related genes from tea (Camellia sinensis L.(O.) Kuntze). (No. BS/YSP-7/1235 dt. 20/05/1997) 3. Bioprospecting genes for adaptive and growth strategies in cold desert plant species of Spiti. (No. BT/PR/502/AGR/08/39/96 dt. 09/10/1997) 4. Collaborative Project (GNDU+IHBT) on Identification, cloning and characterization of stress inducible genes in the grains of wheat at different stages of development. 5. DBT Sponsored Masters Programme in Agricultural Biotechnology.
DST
1995
1998
4.18
INSA
1997
2000
4.99
DBT
Oct, 1997
Oct, 2002
57.17
CSIR
1999
2003
12.50
DBT
60.0 55.71
6. Novel products from Western Himalayas: DBT Antioxidant formulation: cloning of low temperature related cDNA and development of microbial formulations. (BT/PR/3447/AGR/16/285/02-V dt. 01/01/2003) 7. Studying feasibility for production of Panacea novel transgenic plants for increased neutraceutical/pharmaceutical value 8. Modulation of winter dormancy in tea: NTRF Biotechnological approaches 9. Characterization and improvement of tea DBT
2001
2003
3.96
25.0 33.49
10
Sanjay Kumar through biotechnological tools. (No. BT/PR2388/AGR/08/168/2001 dt. 23/11/2001) 10. Characterization and improvement of tea through biotechnological tools. (No. BT/PR2388/AGR/08/168/2001) 11. Niche pathway engineering in tea. (No. 5/258/7/2000-NMITLI dt. 18/03/2001) 12. Exploration and acquisition of specific and targeted nutraceuticals with a back drop of nutrigenomic stevia, tea, and potato species. (No. 30(2050)/CMM014/2003-Finance dated 01/08/2003) 13. Developing cell and tissue engineering for colours and dyes. (No. 30(2050)/CMM002/2003-Finance dated 29/07/2003) 14. Designing Plants and Animals as Bioreactors for Proteins and other products. (No. 30(2050)/CMM004/2003-Finance dated 13/08/2003) 15. Development of medicinal plant chemotypes for enhanced marker and value added compounds. (No. 30(2050)/COR002/2003-Finance dated 29/07/2003) 16. Biosensor for in situ Estimation of Superoxide Radical. (No. DST/TSD/ME/2003/102) 2001 DBT (NMITL I CSIR) CSIR Mission Mode Project CSIR Mission Mode Project CSIR Mission Mode Project CSIR Mission Mode Project DST Mar, 2005 Apr, 2001 Apr, 2002 March, 2007 April, 2005 April, 2007 45.15 157.70 215.90
490.59
157.75
Apr, 2002
April, 2007
424.35
2005
2007
18.30
17. Molecular Cloning and Characterization DBT of Regulatory genes involved in Picrosides Metabolism In Picrorhiza Kurrooa Royle Ex Benth Plants (BT/PR6614/PBD/17/425/2005 dt. 01/03/2006) 18. Pathway engineering and system biology CSIR approach towards homologous and Mission heterologous expression of high-value Mode phytoceuticals (artemisnin, taxanes, Project picrosides, podophyllotoxins, morphine, withanolides). (No.41/3/EFYP/NWP08/2007-RDPD dated 18/22.2.2008
2006
2009
54.20
June 2007
Apr, 2012
446.2
11
Sanjay Kumar 19. Climate change and adaptation of species complexes. 20. Improving carbon and nitrogen sequestration through transgenic approach: a strategy to lower green house gases. DST/IS-STAC/CO2-SR-10/07 dated 27.3.2008 21. Bioprospecting of Buckwheat for Novel Genes and Metabolites of Medicinal and Nutritional Importance. (BOT/PR5136/AGR/16/435/2004 dated 27th Sep, 2007) 22. Bioprospecting Himlayan Bioresources through Transgenic and Nutraceutical Technology (BT/PR8876/NDB/52/68/2007) 23. Developing production system for tea polyphenols and their condensed products. (no.5/258/44/2005-NMITLI dated 20.11.2007) 24. Development of cold inducible synthetic promoter for specific expression of transgenes in plants during cold stress. (BT/PR8924/NDB/52/74/2007 dated 12th Nov., 2007) 25. Developing formulation for improvement of food quality by lowering the level of reactive oxygen species. (BT/PR-10535/FNS/20/374/2008 dated 02.9.2008) 26. Metabolic Engineering of Vinca Alkaloid Pathway 27. Photosynthetic response of altitudinal ecotypes of Rumex nepalensis and Trifolium repens to different concentrations of CO2 with elongated chambers. (SR/SO/PS-07/2010) dated 9.5.2010. 28. Imparting stress-resistance to crop plants by heterologous transfer of high altitude protection mechanisms 29. Heat stress-induced calmodulin-binding proteins in sorghum: characterization, CSIR MMP DST May 2008 Apr 21, 2008 Apr, 2012 Jan, 2012 774.0 35.29
DBT
Nov, 2007
Oct 2010
7.80
DBT
173.27
CSIR NMITLI
142.96
DBT
Nov., 2007
Nov, 2010
31.02
DBT
Sept, 2008
Mar, 2012
101.0
IGSTC DBT
Sanjay Kumar cloning and expression analysis. BT/PR15221/AGR/02/786/2011 30. Genomics of Medicinal plants and Agronomically Important Traits (PlaGen) 31. Probing the Changing Atmosphere and its Impacts in Indo-Gangetic Plains (IGP) and Himalayan Regions (AIM IGPHim) 32. Advanced Instrumentation Solutions for Health Care and Agro-based Applications(ASHA) 33. Plant Diversity: Studying adaptation biology and understanding / exploiting medicinally important plants for useful bioactives (SIMPLE) CSIR CSIR Nov 2012 Jan 2013 Jan 2013 Feb 2013 March 2017 March 2017 March 2017 March 2017 (IHBT 20.10 Lakh) 640.87 90.536
CSIR
100.04
CSIR
2262.3 50
18. TV Show
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Sanjay Kumar Lecture on Climate Change in the progamme Badte Kadam telecasted on 20.2.07 (7:15-7:30 PM) by Shimla Doordarshan, (H.P.), India. Lecture on Climate Change and Himalayan Agriculture telecasted on 26th Jan., 2010 (7:15-7:30 PM) by Shimla Doordarshan, (H.P.), India.
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Sanjay Kumar
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Sanjay Kumar B. Genetic regulation of picrosides, steviosides, shikonins and podophyllotoxin accumulation We completed cloning of metabolic pathway leading to steviosides biosynthesis in Stevia rebaudiana, picrosides biosynthesis in Picrorhiza kurrooa, shikonin biosynthesis in Arnebia euchroma for the first time. Also developed a subtracted EST library of Podophyllum hexandrum for the first time. Our major contribution has been on elucidating the importance of temperature and light in regulating picrosides accumulation in Picrorhiza kurrooa and showed the presence of picrosides in leaves. Previously, rhizomes were harvested for picrosides, because of which whole plants were extracted and the plant is listed as vulnerable species in Red Data Book (Funct. Integr. Genomics DOI: 10.1007/s10142-009-0152-9; Curr. Sci. 2011, 100: 1055-1059). This report has implications in altering the practice for harvesting the plant species and hence conserving the endangered species Picrorhiza kurrooa. Also, for the first time the transcriptome of the temperature response was published (BMC Genomics: 13: 126; 2012) Work on transcript analysis has implications in transplanting the pathway in the plant of choice and in modulating the metabolite levels in innate plant. Also for the first time, shikonins biosynthesis pathway was deciphered and the molecular basis of shikonins biosynthesis was established (BMC Mol. Biol. 11:88. 2010) These original contributions on elucidating the molecular basis of biosynthesis of catechins, picrosides, and shikonins in tea, Picrorhiza kurrooa and Arnebia euchroma, respectively lead the organization to a leadership position. I was invited to deliver a talk and contribute to developing a symposium for International Botanical Congress-2011 in Melbourne, Australia (July, 2011). C. Discovery of novel superoxide dismutase and antioxidants from Himalayan flora Working on a high altitude plant Potentilla atrosanguinea (the plant grows at an altitude of 4,000-4,200 m) we discovered for the first time that the enzyme Cu/Znsuperoxide dismutase (Cu/Zn-SOD) can be autoclaved and can function at temperature ranging from <-10 C to >60 C with temperature optima at 0 C [US Patent 6,485,950 (2002), 7,037,697 (2006)]. The crystal structure of recombinant SOD was worked out (Acta Crystallogr D Biol Crystallogr. 64: 892-901; 2008) and further , the protein was engineered for greater thermostability [Scientific Reports, (Nature Publication Group), in press]. Apart from two major review articles (Crit. Rev. Biotech. 2010 DOI: 10.3109/07388551.2010.490937; J. Mol. Cat B: Enzymatic 68: 129138; 2010), transgenic plants over-expressing the enzyme exhibited tolerance to salt and metal stresses (J. Plant Gen. Trans. 1: 6-10, 2011; J. Plant Physiol. 2010. DOI:10.1016/j.jplph.2009.12.004; J. Plant Res. 2011. DOI:10.1007/s10265-011-04212.).
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Sanjay Kumar
The work has led to the exploitation of the novel enzyme through recombinant protein production in heterologous system e.g. E. coli, thereby leading to the conservation of important Himalayan biodiversity through intervention of biotechnology. D. Discovery of novel carbon sequestration pathway Plants in high altitudes (HA) in Himalayan regions experience high radiation load, low partial pressure of gases, limited soil nitrogen and so on. Working intensely on high altitude biology [Photosynthetica 47: 41-45 (2009); 46:611-614 (2008), 45: 70-74 (2007); 44:136-139 (2006); 43: 195-201 (2005); 42: 303-305 (2004)] Significantly, we published for the first time a carbon sequestering pathway operative in the plants at HA that also conserves nitrogen. Pathway tracing using radiotracer coupled with enzymatic and physiological data led to deciphering the pathway that suggested that phosphoenolpyruvate carboxylase sequestered CO2 from atmosphere and/or that generated metabolically in C3 plants, in parallel to ribulose-1,5-bisphosphate carboxylase/oxygenase, and oxaloacetate thus produced could be additionally channelised for aspartate synthesis using glutamate as a source of ammonia, thus conserving C and N in the HA environment [Photosynth. Res. 88: 63-71, (2006); J. Plant Physiol. 164: 31-38 (2007)]. The work has implications in generating plants with higher carbon and nitrogen fixation/assimilation capabilities. Such plants would perform better even at lower nitrogen application. This has actually been proved through a DST sponsored project. Significantly, the work published in Photosynth. Res. 88: 63-71, (2006) made a separate paragraph in the course on Forest ecology (FR_3104_5104) at the University of Minnesota, USA site (https://wiki.umn.edu/view/FR_3104_5104/Photosynthesis) under the section on photosynthesis wherein one full paragraph has been devoted within environmental factors affecting photosynthesis on Altitude and Carbon Dioxide. This is part of the teaching material on photosynthesis. E. Regulation of winter dormancy in tea Tea in Himalayan region experiences winter dormancy in which the growth of apical buds and the associated two leaves diminishes during winter months. Our work showed that along with protein synthesis (Mol Biol. Rep. 36:537-542 (2009); http://dx.doi.org/10.1007/s11033-008-9211-x (2008)], minimizing oxidative stress could be key to manage winter dormancy in tea wherein superoxide dismutase orchestrating with ascorbate peroxidase and glutathione reductase are critical to lower oxidative stress induced damage and improve plant growth and development [Tree Physiol.,
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Sanjay Kumar 27: 1253-1259 (2007); Biochem. Biophys. Res. Commun. 329: 831-838; 2005; Plant Physiol. Biochem. 43: 383-388 (2005)]. We showed that reduced cell division as a major reason for winter dormancy in tea and the responses to winter dormancy, temperature and plant hormones share gene networks (Mol. Biol. Rep. 2011. DOI: 10.1007/s11033-011-1121-7; Funct. Integr. Genomics, 2011: DOI: 10.1007/s10142-011-0233-4. The work has laid the foundation to raise transgenic plants with reduced winter dormancy.
F. Bioprospecting genes/proteins/processes for tolerance under stress conditions such as temperature and water stress We have contributed immensely towards understanding the mechanism of stress tolerance in terms of shift of metabolic pathways under osmotic stress, basis of temperature rise of water stressed plants, attributed novel function to genes and identification of novel genes and enzymes related to stress. A range of processes and genes were identified. Bioprospecting Himalayan resources, We have cloned and annotated partial and full-length genes, promoters and transcription factors from high altitude flora Rheum australe, Caragana jubata and tea for imparting tolerance to low temperature/drought stress. Several of the genes were found to be novel suggesting novel pathways [Japan Patent 4302532 granted 2009; Mol. Biol. Rep. 37: 933938 (2010); Mol. Biol. Rep. 37: 10031010 (2010); J Bioscience 30: 231-235 (2005); Mol. Biol. Rep. 2011. DOI: 10.1007/s11033-011-1086-6; Gene. 2011 DOI: 10.1016/j.gene.2011.05.014]. These genes/processes are now being transferred to crop plants through Indo-German Project. G. Simplified lab solutions of wide application We have developed a RNA isolation solution (iRIS TM), which is widely used by several labs. Salient feature of iRISTM is that the solution works well with those tissues, wherein commercially available kits fail (BMC Res Notes, 2011. 4:85). The technology has been transferred to Sanmar group (Bangalore genei on 10th Oct, 2007). Also developed a set of primers designed to be universal, irrespective of any plant species, for testing functioning of PCR reactions. These primers have been evaluated by several labs successfully [(ICGEB, NBRI, NCL, Anal Biochem., 335: 330-333 (2004)].
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Sanjay Kumar We developed a gel processing and transfer device (GEPROTED) that ensures un-damaged, intact gel during transfer and transportation of gel from the device onto the membrane while performing northern and southern analyses [European Patent EP1368642 (2007)]. H. Involvement of cyanide resistant alternative respiration in temperature rise of water-stressed plants and fruit ripening. We were the first to propose the role of alternative respiration in fruit ripening and also in thermo-genesis of water-stressed plants (Biochem. Biophys. Res. Commun. 168: 818-822 (1990); J Exp. Bot. 43: 1639-1642 (1992)]. This work has been used as a basis to carry our molecular work on fruit ripening by other group (Planta 197: 569576; 1995) and extending the scope of their conclusions. The group fully supported our results, which were based on biochemical observation, through their molecular results. Our work on alternative respiration has been quoted in 40 other references including in Ann. Rev. Plant Biol., (57: 383-404; 2006).
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Sanjay Kumar
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Sanjay Kumar 12. Tabasum Kawoosa. Submitted 12th Mar, 2012. Cloning and Characterization of Upstream Sequences of Selected Genes of Isoprenoid Biosynthetic Pathway of Picrorhiza kurrooa Royle ex Benth. Faculty of Life Sciences, Guru Nanak Dev University, Amritsar, Punjab. Viva-voce: awaited. 13. A. Paul [2009.Ph.D.(BT)-02]. Submitted 26th Oct, 2012. Transcriptome analysis of winter dormancy in Camellia sinensis (L.) O. Kuntze. Faculty of Life Sciences, Guru Nanak Dev University, Amritsar, Punjab. Viva-voce: awaited. 14. Richard Chalo Muoki (2009-EZ-87; CSIR-TWAS FELLOW). Submitted 7th Nov, 2012. Identification and evaluation of dehydration responsive transcriptome in Camellia sinensis (L.) O. Kuntze. Faculty of Sciences, Panjab University, Chandigarh. Viva-voce: awaited. 15. Arun Kumar Submitted 4th Dec, 2012. Bioprospecting thermostable superoxide dismutase from the flora of western Himalayas. Faculty of Sciences, Panjab University, Chandigarh. Viva-voce: awaited.
25. Patents/Products
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Sanjay Kumar
Patents awarded 1. Kumar, S., Sahoo, R. and Ahuja, P. S. 26 Nov., 2002. A novel isozyme of autoclavable SOD: a process for the identification and extraction of the SOD and use of the said SOD in the cosmetic, food and pharmaceutical composition. US Patents 6,485,950. 2. Kumar, S., Sahoo, R. and Ahuja, P. S. May 2, 2006. awarded. A novel isozyme of autoclavable SOD: a process for the identification and extraction of the SOD and use of the said SOD in the cosmetic, food and pharmaceutical composition. US Patents 7,037,697. 3. Kumar, S. and Dhiraj Vyas. 09th Jun., 2005. Gel processing and transfer device. The device ensures that the agarose gel is not broken during processing and subsequent transfer during northern and southern hybridizations. New Zealand patent 528079/NZ. 4. Kumar, S. and Dhiraj Vyas. 25th July, 2007. Gel processing and transfer device. The device ensures that the agarose gel is not broken during processing and subsequent transfer during northern and southern hybridizations. European Patent (Spain) EP1368642. 5. Kumar, S. and Dhiraj Vyas. 18th Oct, 2007. Gel processing and transfer device. The device ensures that the agarose gel is not broken during processing and subsequent transfer during northern and southern hybridizations. Australian patent no 2001250639 effective from 7th march, 2001 for 20 years. 6. Kumar, S. and Dhiraj Vyas. 28th March, 2009. Gel processing and transfer device. The device ensures that the agarose gel is not broken during processing and subsequent transfer during northern and southern hybridizations. India 233351 7. Kumar, S. and Dhiraj Vyas. 6th July, 2010. Gel processing and transfer device. The device ensures that the agarose gel is not broken during processing and subsequent transfer during northern and southern hybridizations. US patent 7749369. 8. Sharma, P., Kumar, S., and P. S. Ahuja. 2009. Cloning of Novel genes modulated under drought stress conditions in tea [Camellia sinensis (L.) O. Kuntze]. 4302532/JP(01/05/2009) 9. Sharma, P., Kumar, S., and P. S. Ahuja. 2009. Cloning of Novel genes modulated under drought stress conditions in tea [Camellia sinensis (L.) O. Kuntze]. KE290/KE(13/01/2009).
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Sanjay Kumar 10. Sharma, P., Kumar, S., and P. S. Ahuja. 2010. Cloning of Novel genes modulated under drought stress conditions in tea [Camellia sinensis (L.) O. Kuntze]. 238022/IN(18/01/2010) 11. Sharma, P., Kumar, S., and P. S. Ahuja. 2010. Cloning of Novel genes modulated under drought stress conditions in tea [Camellia sinensis (L.) O. Kuntze]. Sri Lanka 13490 (29/10/2010) 12. Sharma, P., Kumar, S., and P. S. Ahuja. 2010. Polyunceotide from a novel gene from drought stress tolerant tea plant. US patent 7718788 (18/05/2010) 13. Bhardwaj, P.K., Sahoo, R., Kumar, S., and Ahuja, P.S. 2011. A gene encoding autoclavable superoxide dismutase and its expression in E. coli. US patent 7,888,088 (15Feb, 2011)
Patents at Scrutiny stage/filed 1. Kumar, S., Rajesh Kumar Gupta and P. S. Ahuja. United States Patent 20060135757 dated June 22, 2006. DNA sequence in plant Caragana jubata with freeze tolerance 2. Kumar, S., Lakhvir Lal and P. S. Ahuja. Patent filed; US, SL, CN 095NF2001. 30.03.2001. Cloning of novel gene sequences expressed and repressed during winter dormancy in the apical buds of tea (Camellis sinensis L.(O.) Kuntze) bush. 3. Ghawana, S., Singh, K. Raizada, J, Rani, A, Bhardwaj, P.K. and Kumar, S. Applied 2004, Filed 30.03.06 0344 NF 2004/IN. A method for rapid isolation of RNA and kit thereof. 4. Ghawana, S., Singh, K. Raizada, J, Rani, A, Bhardwaj, P.K. and Kumar, S. filed USA 12/295001 dated 29.09.2008. A cost effective less hazardous two solution system for rapid isolation of RNA. 5. Bhardwaj, P.K., Sahoo, R., Kumar, S., and Ahuja, P.S. 0038 NF 2006/IN Dated 31.03.06. A gene encoding autoclavable superoxide dismutase and its expression in E. coli. 6. Pardeep Kumar Bhardwaj, Arun Kumar, Amit Kishore, Sanjay Ghawana, Arti Rani , Kashmir Singh, Harsharan Singh, Ravi Shankar Singh, Hitesh Kumar, Payal Sood, Som Dutt, Sanjay Kumar, Paramvir Singh Ahuja. 08 Oct., 2009. Method of cloning stable stress tolerant superoxide dismutase using degenerate primers. WO/2009/122441.
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Sanjay Kumar 7. Pardeep Kumar Bhardwaj, Arun Kumar, Amit Kishore, Sanjay Ghawana, Arti Rani , Kashmir Singh, Harsharan Singh, Ravi Shankar Singh, Hitesh Kumar, Payal Sood, Som Dutt, Sanjay Kumar, Paramvir Singh Ahuja . A method for cloning functional gene of copper/zinc superoxide dismutases using oligonucleotide primers. (0035nf2008). Filed in India 28th March, 2008. 8. Pardeep Kumar Bhardwaj, Arun Kumar, Amit Kishore, Sanjay Ghawana, Arti Rani , Kashmir Singh, Harsharan Singh, Ravi Shankar Singh, Hitesh Kumar, Payal Sood, Som Dutt, Sanjay Kumar, Paramvir Singh Ahuja . A method for cloning functional gene of copper/zinc superoxide dismutases using oligonucleotide primers. Filed in USA 12/935881 dated 30/9/2010. 9. Pardeep Kumar Bhardwaj, Arun Kumar, Amit Kishore, Sanjay Ghawana, Arti Rani , Kashmir Singh, Harsharan Singh, Ravi Shankar Singh, Hitesh Kumar, Payal Sood, Som Dutt, Sanjay Kumar, Paramvir Singh Ahuja . A method for cloning functional gene of copper/zinc superoxide dismutases using oligonucleotide primers. (0035nf2008). Filed in European patent office 909727645.5 dated 11.10.2010 10. Ravi Shankar Singh, Sanjay Kumar. A composition for remoal of colours and inhibitors from plant tissues to isolate RNA. Filed in India 2832 DEL 2008 dated 15.12.2008. 11. Ravi Shankar Singh, Sanjay Kumar. 2008. A composition and method for removal of colours and inhibitors from tissues to isolate RNA. 0089 NF 2008/WO filed via application number PCT/IN2009/000612 dated 28.10.2009. 12. Anish Kaachra, Surender Kumar Vats, Paramvir Singh Ahuja, Sanjay Kumar. 2011. A method for enhancing status of carbon, nitrogen, biomass and yield of plants. Filed via application number 1143del 2011 dated 19.4.2011 (NF0057/2011). 13. Arun Kumar, Som Dutt, Paramvir Singh Ahuja, and Sanjay Kumar. An autoclave stable recombinant Cu/Zn superoxide dismutase with enhanced thermoflexibility (NF0050/2011). Filed via application number 1031del 2011 dated 11.4.2011. 14. Sahoo, Rashmita, Kumar, Sanjay, Ahuja, Paramvir Singh, Bhardwaj, Pradeep Kumar. 7th Feb 2013 (date of acceptance). Superoxide dismutase (SOD) gene and a method of identifying and cloning thereof (0038NF2006; Australian Patent Application No. 2006341292).
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Sanjay Kumar Transferred technology of the kit for isolation of RNA to Sanmar Group (Bangalore Genei), Chennai, India on 10th October, 2007.
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Publications 1. Bhardwaj, P.K., Kapoor, R., Deep Mala, Bhagwat, G., Acharya, V., Singh, A. K., Vats, S.K., Ahuja, P.S., Kumar, S. 2013. Braving the attitude of altitude: Caragana jubata at work in cold desert of Himalaya. Scientific Reports (Nature Publishing Group) 3: 1022; DOI: 10.1038/srep01022. 2. Kumar, A., Dutt, S., Bagler, G., Ahuja, P.S., and Kumar, S. 2012. Engineering a thermo-stable superoxide dismutase functional at sub-zero to >50 C, which also tolerates autoclaving. Scientific Reports (Nature Publishing Group) 2: 387; DOI:10.1038/srep00387. 3. Paul, A., and Kumar, S. 2012. Dehydrin2 is a stress-inducible, whereas Dehydrin1 is constitutively expressed but upregulated gene under varied cues in tea [Camellia sinensis (L.) O. Kuntze]. Molecular Biology Reports. DOI: 10.1007/s11033-012-2466-2 4. Singh, H., Gahlan, P., Kumar, S. 2012. Cloning and expression analysis of ten genes associated with picrosides biosynthesis in Picrorhiza kurrooa. Gene. DOI:10.1016/j.gene.2012.12.004. 5. Pal, A.K., Acharya, K., Vats, S.K., Kumar, S. and Ahuja, P.S. 2012. Overexpression of PaSOD in transgenic potato enhances photosynthetic performance under drought. Biologia Plantarum (published on line). 6. Kumar, R., Kaundal, Vats, S.K., and Kumar, S. 2012. Effect of thermal and radiation regimes on phenology and dry matter production of white clover (Trifolium repens) in western Himalayas. Journal of Agrometeorology 14: 138-142. 7. Kumar, H., Singh, K., and Kumar, S. 2012. 2C-methyl-D-erythritol 2,4cyclodiphosphate synthase from Stevia rebaudiana Bertoni is a functional gene. Molecular Biology Reports. DOI: 10.1007/s11033-012-1998-9. 8. Kumar, S., Kalra, S., Kumar, S., Kaur, J., Singh, K. 2012. Differentially expressed transcripts from leaf and root tissue of Chlorophytum borivilianum: a plant with high medicinal value. Gene, doi: 10.1016/j.gene.2012.09.046 9. Acharya, K. Pal, A.K., Gulati, A., Kumar, S., Singh, A.K., and Ahuja, P.S. Overexpression of Camellia sinensis thaumatin-like protein, CsTLP in potato confers enhanced resistance to Macrophomina phaseolina and Phytophthora infestans infection. Molecular Biotechnology, DOI 10.1007/s12033-0129603-y. 10. Singh, R. S., and Kumar, S. 2012. A protocol to remove colored metabolites and other inhibitors from plant tissues to facilitate RNA isolation suitable for downstream applications. Biotechnology Progress. DOI: 10.1002/btpr.1577. 11. Paul, A., Muoki, R.C., Singh, K. and Kumar, S. 2012. CsNAM-like protein encodes a nuclear localised protein and responds to varied cues in tea [Camellia sinensis (L.) O. Kuntze]. Gene. 502: 69-74. DOI: 10.1016/j.gene.2012.04.017.
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Sanjay Kumar 12. Muoki, R.C., Paul, A., and Kumar, S. 2012. A shared response of thaumatin like protein, chitinase and late embryogenesis abundant protein3 to environmental stress in tea [Camellia sinensis (L.) O. Kuntze]. Functional and Integrative Genomics. DOI: 10.1007/s10142-012-0279-y. 13. Gahlan, P., Singh, H.R., Shankar, R., Sharma, N., Kumari, A., Chawla, V., Ahuja, P.S., and Kumar, S. 2012. De novo sequencing and characterization of Picrorhiza kurrooa transcriptome at two temperatures showed major transcriptome adjustments. BMC Genomics: 13: 126. doi:10.1186/1471-216413-126 14. Chawla, A., Yadav, P.K., Uniyal, S.K., Kumar, A., Vats, S.K., Kumar, S. and Ahuja, P. S. 2012. Long-term ecological and biodiversity monitoring in the western Himalaya using satellite remote sensing. Current Science 102: 11431156. 15. Rani, A., Singh, K., Ahuja, P. S., Kumar, S. 2012. Molecular regulation of catechins biosynthesis in tea (Camellia sinensis (L.) O. Kuntze). Gene 495: 205-210. DOI: 10.1016/j.gene.2011.12.029. 16. Kumar H., Kaul, K., Gupta-Bajpai, S., Kaul, V.K., Kumar, S. 2012. A comprehensive analysis of fifteen genes of steviol glycosides biosynthesis pathway in Stevia rebaudiana (Bertoni). Gene 492: 276-284. DOI: 10.1016/j.gene.2011.10.015. 17. Muoki, R.C., Paul, A., Kumari, A., Singh K., and Kumar, S. 2011. An improved protocol for the isolation of RNA from roots of tea (Camellia sinensis (L.) O. Kuntze). Molecular Biotechnology. DOI: 10.1007/s12033011-9476-5 18. Paul, A. and Kumar, S. 2011. Responses to winter dormancy, temperature and plant hormones share gene networks. Functional and Integrative Genomics. DOI: 10.1007/s10142-011-0233-4. 19. Vats, S.K., Kumar, S., and Ahuja, P.S. 2011. CO2 sequestration in plants: lesson from divergent strategies. Photosynthetica 49: 481-496. 20. Paul, A., Lal, L., Ahuja, P.S. and Kumar, S. 2011. Alpha-tubulin (CsTUA) upregulated during winter dormancy is a low temperature inducible gene in tea (Camellia sinensis (L.) O. Kuntze). Molecular Biology Reports. DOI: 10.1007/s11033-011-1121-7 21. Bhardwaj, P.K., Kaur, J., Sobti, R.C. and Kumar, S. 2011. Identification and expression analysis of CjLTI, a novel low temperature responsive gene from Caragana jubata. Molecular Biology Reports. DOI: 10.1007/s11033-0111086-6 22. Bhardwaj, P.K., Kaur, J., Sobti, R.C., Ahuja, P.S. and Kumar, S. 2011. Lipoxygenase gene in Caragana jubata responds to low temperature, abscisic acid, methyl jasmonate and salicylic acid. Gene. DOI: 10.1016/j.gene.2011.05.014. 23. Gill, T., Dogra, V., Kumar, S., Ahuja, P. S. and Sreenivasulu, Y. 2011. Protein dynamics during seed germination under copper stress in Arabidopsis over-expressing Potentilla superoxide dismutase. Journal of Plant Research DOI:10.1007/s10265-011-0421-2. 24. Ghawana, S., Paul, A., Kumar, H., Kumar, A., Singh, H., Bhardwaj, P.K.,
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Sanjay Kumar Rani, A., Singh, R.S., Raizada, J., Singh, K., and Kumar, S. 2011. An RNA isolation system for plant tissues rich in secondary metabolites. BMC Research Notes 4: 85. DOI:10.1186/1756-0500-4-85. 25. Singh, K., Rani, A., Ahuja, P.S. and Kumar, S. 2010. Present status and future prospects on catechins biosynthesis in tea. Journal of Plant Biology 37: 223246. 26. Singh, H., Gahlan, P., Dutt, S., Ahuja, P.S. and Kumar, S. 2011. Why uproot Picrorhiza kurrooa, an endangered medicinal herb? Current Science 100: 1055-1059. 27. Rani, A., Vats, S.K., Sharma, M., and Kumar, S. 2011. Catechin promotes root and shoot growth of Arabidopsis thaliana (Col-0) seedlings with concomitant changes in vascular system, IAA level and the net photosynthetic rate. Biologia Plantarum 55: 779-782. DOI: 10.1007/s10535-011-0071-1. 28. Singh, R. S., Gara, R. K., Bhardwaj, P. K., Malik, S., Kumar, R., Sharma, M., Ahuja P. S., and Kumar, S. 2010. Expression of 3-hydroxy-3-methylglutarylCoA reductase, p-hydroxybenzoate-m-geranyltransferase and genes of phenylpropanoid pathway exhibits positive correlation with shikonins content in arnebia [Arnebia euchroma (Royle) Johnston]. BMC Molecular Biology 11:88. doi:10.1186/1471-2199-11-88. 29. Gill, T., Kumar, S., Ahuja, P. S. and Sreenivasulu, Y. 2010. Over-expression of Potentilla superoxide dismutase improves salt stress tolerance during germination and growth in Arabidopsis thaliana. Journal of Plant Genetics & Transgenics 1: 6-10 30. Bafana, A., Dutt, S., Kumar, A., Kumar, S., Ahuja, P. S. 2010. The basic and applied aspects of superoxide dismutase. Journal of Molecular Catalysis B: Enzymatic 68: 129138. 31. Bafana, A. P., Dutt, S., Kumar, S., Ahuja, P. S. 2010. Superoxide dismutase: An industrial perspective. Critical Reviews in Biotechnology 31: 65-76. DOI: 10.3109/07388551.2010.490937. 32. Kawoosa, T., Singh, H. Kumar, A., Sharma, S. K., Devi, K., Dutt, S., Vats, S. K., Sharma, M., Ahuja, P. S., and Kumar, S. 2010. Light and temperature regulated terpene biosynthesis: hepatoprotective monoterpene picroside accumulation in Picrorhiza kurrooa. Functional and Integrative Genomics 10:393404; DOI: 10.1007/s10142-009-0152-9. 33. Bhardwaj, P.K. Ahuja, P.S. and Kumar, S. 2010. Characterization of gene expression of QM from Caragana jubata, a plant species that grows under extreme cold. Molecular Biology Reports 37: 10031010. DOI: 10.1007/s11033-009-9791-0. 34. Ghawana, S., Kumar. S., Ahuja, P. S. 2010. Early low-temperature responsive mitogen activated protein kinases RaMPK1 and RaMPK2 from Rheum australe D. Don respond differentially to diverse stresses. Molecular Biology Reports. 37: 933938. DOI: 10.1007/s11033-009-9726-9.
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Sanjay Kumar 35. Gill, T., Sreenivasulu, Y., Kumar, S., and Ahuja, P. S. 2010. Over-expression of superoxide dismutase exhibits lignification of vascular structures in Arabidopsis thaliana. Journal of Plant Physiology 167: 757-760. DOI:10.1016/j.jplph.2009.12.004. 36. Singh, K., Rani, A., Paul, A., Dutt, S., Joshi, R. Gulati, A., Ahuja, P. S., Kumar, S. 2009. Differential display mediated cloning of anthocyanidin reductase gene from tea (Camellia sinensis) and its relationship with the concentration of epicatechins. Tree Physiology. 29: 837-46. DOI: 10.1093/treephys/tpp022. 37. Rani, A., Singh, K., Sood, P., Kumar, S., Ahuja, P. S. 2009. pCoumarate:CoA ligase as a key gene in the yield of catechins in tea [ Camellia sinensis (L.) O. Kuntze]. Functional and Integrative Genomics 9: 271275. DOI: 10.1007/s10142-008-0098-3. 38. Singh, K., Kumar, S., Rani, A., Gulati, A., Ahuja, P. S. 2009. Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan 3-ols) accumulation in tea. Functional and Integrative Genomics 9: 125134. DOI 10.1007/s10142-008-0092-9. 39. Virdi, A. S., Thakur, A., Som Dutt, Kumar, S., Singh, P. 2009. A sorghum 85 kDa heat stress-modulated protein shows calmodulin-binding property and cross-reactivity to anti Neurospora crassa-Hsp 80 antibodies. FEBS Letters 583: 767770. 40. Singh, K., Paul, A., Kumar, S., Ahuja, P.S. 2009. Cloning and differential expression of human Wilm's tumor suppressor homologue, QM-family protein (L10) isolated from tea [Camellia sinensis (L.) O. Kuntze]. Molecular Biology Reports 36: 921-927. http://dx.doi.org/10.1007/s11033-008-9264-x. 41. Singh, K., Kumar, S., Ahuja, P.S. 2009. Differential expression of Histone H3 gene in tea (Camellia sinensis (L.) O. Kuntze) suggests its role in growing tissue. Molecular Biology Reports 36: 537-542. 42. Singh, K., Kumar, S., Yadav, S.K., Ahuja, P. S. 2009. Characterization of Dihydroflavonol 4-reductase (DFR) cDNA in tea (Camellia sinensis (L.) O. Kuntze). Plant Biotechnology Reports 3:95-101. 43. Vats, S.K., Kumar, N., and, Kumar, S. 2009. Gas exchange response of Barley and Pea varieties to altitude variation in Himalaya. Photosynthetica 47: 41-45. 44. Yogavel, M., Mishra, P.C., Gill, J., Bhardwaj, P.K., Dutt, S., Kumar, S., Ahuja, P.S., Sharma, A. 2008. Structure of a superoxide dismutase and implications for copper-ion chelation. Acta Crystallographica Section D Biological Crystallography D64, 892901. 45. Singh, K., Rani, A., Kumar, S., Sood, P., Mahajan, M., Yadav, S.K., Singh, B., Ahuja, P. S. 2008. An early gene of flavonoid pathway, flavanone 3hydroxylase, exhibits a positive relationship with catechins content in tea (Camellia sinensis (L.) O. Kuntze). Tree Physiology 28:13491356. 46. Kumar, N., Vats, S.K., Kumar, S. and Ahuja, P.S. 2008. Altitude related changes in activities of some enzymes in Rumex nepalensis. Photosynthetica 46: 611-614. 47. Singh, G., Jain, M., Kulshreshtha, R., Khurana, J.P., Kumar, S., Singh, P.
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Sanjay Kumar 2007. Expression analysis of genes encoding translation initiation factor 3 subunit g (TaeIF3g) and vesicle-associated membrane protein-associated protein (TaVAP) in drought tolerant and susceptible cultivars of wheat. Plant Science 173 660669. 48. Vyas, D., Kumar, S., Ahuja, P.S. 2007. Tea (Camellia sinensis) clones with shorter periods of winter dormancy exhibit lower accumulation of reactive oxygen species. Tree Physiology 27: 1253-1259. 49. Kumar, N., Kumar, S. and Ahuja, P.S. 2007. Different responses of wild and cultivates plants to high irradiance. Photosynthetica 45: 70-74. 50. Kumar N., Vyas, D. and Kumar, S. 2007. Plants at high altitude exhibit higher component of alternative respiration. Journal of Plant Physiology 164: 31-38 51. Olmos, E., Kiddle, G., Pellny, T.K., Kumar, S., and Foyer, C.H. 2006. Modulation of plant morphology, root architecture, and cell structure by low vitamin C in Arabidopsis thaliana Journal of Experimental Botany 57: 1645 - 1655. 52. Kumar, N., Kumar, S., Vats, S.K., and Ahuja, P. S. 2006. Effect of altitude on the primary products of photosynthesis and the associated enzymes in barley and wheat. Photosynthesis Research (DOI: 10.1007/s11120-005-9028-6) 88: 63-71. 53. Vats, S.K. and Kumar, S. 2006. Photosynthetic characteristics of Podophyllum hexandrum Royle collected from different altitudes in Himalaya. Photosynthetica 44:136-139. 54. Sharma A.D., Kumar S., and Singh, P. 2006. Expression analysis of a stressmodulated transcript in drought tolerant and susceptible cultivars of sorghum (Sorghum bicolor). Journal of Plant Physiology 163: 570-576. 55. Pavet, V., Olmos, E., Kiddle, G., Mowla, S., Kumar, S., Antoniw,J., Alvarez, M.E., Foyer, C.H. 2005. Ascorbic acid deficiency activates cell death and disease resistance responses in Arabidopsis thaliana. Plant Physiology 139: 1291-1303. 56. Vyas, D., and Kumar, S. 2005. Tea (Camellia sinensis (L.) O. Kuntze) clone with lower period of winter dormancy exhibits lesser cellular damage in response to low temperature. Plant Physiology and Biochemistry (France) 43: 383-388. 57. Vyas, D., and Kumar, S. 2005. Purification and partial characterization of a low temperature responsive Mn-SOD from tea ( Camellia sinensis (L.) O. Kuntze). Biochemical and Biophysical Research Communications 329: 831838. 58. Sharma, P. and Kumar, S. 2005. Differential display mediated identification of three drought-responsive expressed sequence tags in tea (Camellia sinensis (L.) O. Kuntze). Journal of Biosciences 30: 231-235. 59. Kumar, N., Kumar, S. and Ahuja, P.S. 2005. Photosynthetic characteristics of Hordeum, Triticum, Rumex, and Trifolium at contrasting altitudes. Photosynthetica 43: 195-201. 60. Singh, K., Raizada, J., Bhardwaj, P., Ghawana, S., Rani, A., Singh, H., Kaul, K., and Kumar, S. 2004. 26S rRNA based internal control gene primer pair for reverse transcription-polymerase chain reaction-based quantitative expression
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Sanjay Kumar studies in diverse plant species. Analytical Biochemistry 335: 330-333 61. Kumar, N., Kumar, S. and Ahuja, P.S. 2004. Differences in the activation state of ribulose-1,5 -bisphosphate carboxylase/oxygenase in barley, pea and wheat at two altitudes. Photosynthetica 42: 303-305. 62. Pandey, S., Kumar, S. and Nagar, P. K. 2003. Photosynthetic performance of Ginkgo biloba L. grown under high and low irradiance. Photosynthetica 41: 505-511. 63. Singh, G., Kumar, S. and Singh, P. 2003. A quick method to isolate RNA from wheat and other carbohydrate-rich seeds. Plant Molecular Biology Reporter 21: 93a-93f 64. Vyas, D., Sahoo, R. and Kumar, S. 2002. Possible mechanism and implications of phenolics mediated reduction of XTT (sodium, 3'- [1[ phenylamino-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6-nitro) benzenesulfonic acid hydrate). Current Science, 83:1588-1592 . 65. Lal, L., Sahoo, R., Gupta, R. K., Sharma, P., and Kumar, S. 2001. RNA isolation from tea leaves, a high polyphenolic containing tissue, and extension of the procedure to diverse plant species. Plant Molecular Biology Reporter, 19: 181a-181f. 66. Sahoo, R, Kumar, S., and Ahuja, P. S. 2001. Induction of new isozyme of superoxide dismutase at low temperature in Potentilla atrosanguineae Lodd. Variety argyrophylla (wall. Ex. Lehm) Griers. Journal of Plant Physiology, 158: 1093-1097. 67. Kumar, N. and Kumar, S. 2001. Differential activation of ribulose-1,5bisphosphate carboxylase/oxygenase in non-radiolabelled versus radiolabelled sodium bicarbonate. Current Science, 80: 333-334. 68. Kumar, S. 2000. Metabolic Synthesis of Water in Plants and its Physiological Implications. Current Science, 79: 1054-1057. 69. Kumar, S., and Acharya, S.K. 1999. 2,6-Dichloro-phenol indophenol prevents switch-over of electrons between cyanide sensitive and insensitive pathway of mitochondrial electron transport chain in the presence of inhibitors. Analytical Biochemistry, 268: 89-93. 70. Bhaglal, P., Singh, P., Bhullar, S.S., Kumar, S. 1998. Drought stress induced accumulation of wheat germ agglutinin in the developing embryos of wheat (Triticum aestivum L.) and its likely role in vivo. Journal of Plant Physiology, 153: 163-166. 71. Kumar, S., Kundu, P.K. and Ahuja, P.S. 1996. Caution on the use of oxygen electrode-based assay for superoxide dismutase activity in crude preparations. Current Science, 71: 818-819. 72. Joshi, C.P., Kumar, S. and Nguyen, H.T. 1996. Application of modified differential display technique for cloning and sequencing of the 3' region from three putative members of wheat hsp70 gene family. Plant Molecular Biology, 30: 641-646. 73. Kumar, S. and Sinha, S.K. 1994. Possible role of alternative respiration in temperature rise of water stressed plants. Journal of Biosciences, 19: 331-338. 74. Hebbar, K.B., Kumar, S. and Sinha, S.K. 1994. Action potential- a possible
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Sanjay Kumar signal in root to shoot communication caused by water deficits around roots of sunflower seedlings. Current Science, 66: 936-938. 75. Kumar, S. and Sinha, S.K. 1992. Alternative respiration and heat production in ripening banana fruits (Musa paradisiaca). Journal of Experimental Botany, 43: 1639-1642. 76. Kumar, S., Patil, B.C. and Sinha, S.K. 1990. Cyanide resistant respiration is involved in temperature rise in ripening mangoes. Biochemical and Biophysical Research Communications, 168: 818-822. 77. Kumar, S. and Gupta, R.K. 1986. Influence of different leaf water potentials on photosynthetic carbon metabolism in sorghum. Photosynthetica, 20: 391396. 78. Kumar, S. and Gupta, R.K. 1986. Photorespiratory CO2 evolution and 14 Cglycine metabolism under different leaf water potentials in sorghum. Photosynthetica, 20: 401-404.
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Sanjay Kumar Modification and Characterization & 30th Annual Meeting of Plant Tissue Culture Association (India). Institute of Himalayan Bioresource Technology Palampur, April 3-4, 2009. Abstract no. 102. Page no. 64. 7. Manika Sharma, Ramesh Singh Pal, S.K. Vats, Sanjay Kumar, Paramvir Singh Ahuja. Molecular cloning and gene expression analysis of the pathway involved in podophyllotoxin biosynthesis in Podophyllum hexandrum Royle. National Symposium on Plant Propagation, conservation, Modification and Characterization & 30th Annual Meeting of Plant Tissue Culture Association (India). Institute of Himalayan Bioresource Technology Palampur, April 3-4, 2009. Abstract no. 83. Page no. 53. 8. Tabasum Kawoosa, Amit Kumar, Harsharan Singh, Sunil Kumar Sharma, Kiran Devi, Som Dutt, Sanjay Kumar, Madhu Sharma, Paramvir Singh Ahuja. Molecular regulation of picrosides biosynthesis in Picrorhiza kurrooa Royle ex Benth. 3rd J&K Science Congress 26-28 Feb., 2008 in Jammu University, J&K. (Won first prize). 9. Harsharan Singh, Amit Kumar, Tabasum Kawoosa, Som Dutt, Sanjay Kumar, Paramvir Singh Ahuja Cloning and characterization of genes involved in picrosides biosynthesis in Picrorhiza kurrooa Royle ex Benth. 3rd J&K Science Congress 26-28 Feb., 2008 in Jammu University, J&K. (Won second prize) 10. Surabhi, Giridara-Kumar; Sanjay Kumar; Alam, Nausheen; Caragea, Doina; Lu, Nanyan; Hurt Amy; Loretta , Johnson; Shah, Jyoti, 2008. Chronic and transient effects of nitrogen saturation on root processes in a dominant prairie grass Andropogon gerardii: Linking gene expression profiles and ecological responses. PRELIM7685, Plant Biology 2008. 11. Giridara-Kumar Surabhi, Sanjay Kumar, Dan DeLaughter, Katie Clowers, Nausheen Alam, Loretta Johnson, Jyoti Shah (2007) Environmental and ecological controls on gene expression of root processes in prairie plants, presented at conference on convergence of genomics and the land-grant mission: emerging trends in the application of genomics in agricultural research, Purdue University, IN,USA. Sept. 10-12. (http://www.entm.purdue.edu/conference/program.html). 12. Giridara-Kumar Surabhi, Sanjay Kumar, Dan DeLaughter , Katie Clowers, Nausheen Alam, Loretta Johnson, Jyoti Shah. 2007. Environmental and
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Sanjay Kumar 13. Singh, K., Kumar, S., Sharma., V., Gulati., A., and Ahuja, P.S. 2004. Understanding the regulation of catechin biosynthesis in tea ( Camellia sinensis (L.) O. Kuntze). Annual meeting of American Society of Plant Biologists, Orlando, July 24-28, 2004; Abstract no. 248. 14. Kumar, S., Vir, N., Chakrabarty, D.N. and Gupta, A.K. 1994. Lower maintenance foliage: source or sink in tea ( Camellia sinensis L. (O.) Kuntze) Bush. National Symposium on " Newer Challanges in Agriculture, Horticulture and Industry: The role of Physiologist and Biochemist", University of Agricultural Sciences, GKVK Campus, Bangalore, India, January 11-13, 1994. 15. Kumar, S. and Palni, L.M.S. 1993. Biochemical and physiological evaluation of floral senescence in intact and cut spikes of gladiolus var. aldebaran. 3rd Conference of APPSC and Annual Convention of PGRSI, Gujarat University, Ahmedabad, Apr 18-20, 1993. 16. Kumar, S. and Palni, L.M.S. 1993. Possible involvement of alternative respiration in regulating senescence of gladiolus florets. National Symposium on "Physiological and Biochemical Aspects of Crop Improvement", NBRI, Lucknow, India, March 24-25, 1993. (Won best presentation award). 17. Kumar, S. and Palni, L.M.S. 1993. Alternative respiration and floral senescence: an assessment. National Conference on Advances in Molecular and Plant Physiological Researches in Crop Improvement and Annual Convention of the Indian Society for Plant physiology, Kumarganj, Faizabad, India, Feb 22- 25, 1993. 18. Kumar, S., Patil, B.C., Palni, L.M.S. and Sinha, S.K. 1992. Post harvest changes in alternative respiration in mango fruits. 4th International Mango Symposium, Miami Beach, Florida, USA, July 5-10, 1992. 19. Kumar, S. and Sinha, S.K. 1988. Hydrogen peroxide generation by crop plants. Poster presented at First International Congress of Plant Physiology, New Delhi, Feb 15-20, 1988.
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