STEMBiologyMicroscopeLabWriteup JacksonBabbandJoesphCupchack September13th,2011 6thHour

PurposeAndMaterials
Thislabisanintroductiontothedigitalmicroscopestheclassisgoingtobeusing throughouttheyear. Materials: Paper Pencil Computer DigitalMicroscopewith40,100,400,and1000xmagnification MoticImagesPlus2.0 USBconnectiontothemicroscope Woolthread Nylonthread Aprintedlowercasee Darkhair Silica Twoobjectsofchoice(Aleaforlintforexample) Awordprocessor Coverslip Water Slide Dropper Twoindexcards

Procedure
1. Getmaterialsandaspacetowork 2. Putwoolthreadonaslideandplaceonstage 3. Startat40xandmoveupto100x 4. CaptureimageusingMotic 5. Saveimage 6. Putnylonthreadonslide 7. Addadropofwaterontothethread 8. Placecoverslipoverit 9. Focuson40xthenmoveto400x 10. CaptureimageusingMotic 11. Saveimage 12. Placeaprintedlettereonslide 13. Addadropofwaterontothee 14. Placecoverslipoverit 15. Examineat100x 16. CaptureimageusingMotic 17. Saveimage 18. Placehaironslide 19. Addadropofwaterontothehair 20. Placecoverslipoverit 21. Rotateobjectivelensesto400x 22. TakeapicturewithMotic 23. Savetheimage 24. Foldoneindexcardinhalfsoitismoresquare 25. Cutasmalltriangleinthefoldedside 26. Openupandtapetheopeningandstickyourobjectofchoiceontothetape 27. Tapetheothersidesotheobjectiscoveredwithtape 28. Placethefirstthingofchoiceundermicroscope 29. Zoomto100x 30. TakeapicturewithMotic 31. Savetheimage 32. Repeatsteps24to31withthesecondobject

WoolThread100x

Drawings NylonThread400x

Lettere100x

DarkHair400x

Silica400x

Leaf100x

PocketLint100x

Questions 1.Whatisthedifferencebetweenthewoolthreadandthenylon? Thewoolthreadlookedbetterupcloseandwaseasiertoseesinceitwasntwhite. Thenylonthreadlookedorderly,whilethewoolwasintertwinedandfrayed. 2.Whathappenedtotheletterewhenputunderamicroscope? Itturnedupsidedownandbackwards,butalsoitdidntlookasfilledinasitseemed.

3.Whilelookingatthee,turnthenorthstageknobtotheright.Whathappened? Itlookedlikeitmovedtotheleft. 4.Whatissilicausedfor? Itisusedtoabsorbmoisture. 5.Whatisonindexcard#1,wheredidyoufindit,andwhydidyouchoosetolookatthis item? Thereisaleafonthecard.Ifounditoutsidenearthewoods.Ichosetolookatit becauseIthoughtlookingatthestructureofaleafwouldbeneat. 6.Whatisonindexcard#2,wheredidyoufindit,andwhydidyouchoosetolookatthis item? Itwaslint,ifounditinmypocket,andichoseitbecauseitwassmallandprobably hadaninterestingstructure. 7.Whattwopartsofthemicroscopedoyouusetocarryit? Thearmandbase. 8.Whattypeofmicroscopedidyouuseinthislab? Adigitalmicroscope. 9.Whydoyouneedtofocusat100xbeforeyoucangoto400x? becauseitishardertofocusat400x 10.Whymustyouonlyusecoarseadjustmentonlowpower? Itwillnotfocusrightifyouuseitonhighpower 11.Whatthreethingsmustyoudotothemicroscopebeforeputtingitaway? 1.Turnitoff 2.Unplugandwrapthecord 3.Setthenosepiecesothe40xobjectiveisactive.

Conclusion Duringthislab,IlearnedmostofwhatIknownowaboutmicroscopes.Itwasimportant becauseweusedmicroscopes,andifIdidntknow,Iwouldnothavebeenabletofinish thislab.Myfavoritethingaboutthislabwasseeingup1000xthesizeofsmallobjects.I didntlikemydecisiontopickathickleaftoexamineinsteadofathinone.Nothingreally wentwronginthelab.IfIhadanotherindexcard,Iwouldputgraphiteunderthemicroscope becauseitismyfavoriteformofcarbon.