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Laboratory Exercise No.2 The Compound Microscope Introduction A compound microscope has two sets of lenses. The lens you look through is called the ocular. The lens near the specimen being examined is called the objective. The objective lens is one of three or four lenses located on a rotating turret above the stage, and that vary in magnifying power. The lowest power is called the low power objective (LP), and the highest power is the high power objective (HP). You can determine the magnifying power of the combination of the two lenses by multiplying the magnifying power of the ocular by the magnifying power of the objective that you are using. The field of view is the maximum area visible through the lenses of a microscope, and it is represented by a diameter. When you are viewing an object under high power, it is sometimes not possible to determine the field of view directly. The higher the power of magnification, the smaller the field of view. The diameter of the field of view under high power must be calculated using the following equation. diameter (LP) x magnification of LP objective magnification of HP objective Objectives 1. To identify different parts of a light microscope. 2. To identify magnifying power of microscope and the size of the field of view. 3. To estimate the size of the specimen in micrometers (m). Materials Light Microscope Specimen (small printed letter E Ruler = diameter (HP)

Pipette Glass slide Cover glass/slip

Procedure A 1. Draw a light microscope and label the different parts and write the purpose of each part. Procedure B 1. Using ruler, measure the size of the specimen before it is magnified. 2. Using forceps, carefully place the specimen in the center of the slide 3. Add two or three drops of water with your pipette. 4. Place a clean cover glass over the specimen. 5. Observe the specimen under low power objective. 6. Place a millimeter ruler along the right side of the stage. 7. Look into the microscope with one eye and at the millimeter ruler with the other eye. Both ruler and the specimen can be seen at the same time. 8. Take the measurement of the image. 9. Compute the field of view. 10. Observe also the specimen in high power objective. 11. Observe other specimens under the microscope.

DATA SHEET Light Microscope

Parts 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.

Purpose

1. List the power of the ocular and objective lenses of microscope to be used. Calculate the magnifications for the combination noted. Ocular Objectives Magnification

2. Write the term that matches each meaning. a) Tissue used to clean lenses b) Objective with the least working distance c) Slide with an attached cover glass d) Objective with the largest field

________________________ ________________________ ________________________ ________________________

3. Indicate the direction the image moves when the slide is moved: To the left ________________________ Away from you ________________________ 4. Draw the letter E as it appears when observed with each the following:

Actual

4x

10x

40x

5. Indicate the estimated field of view at each Total magnification of the microscope Magnification Field of View