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Chemicals required:
1) Chlorpromazine hydrochloride tablets, 2) Dil. Hydrochloric acid , 4)Methanol.
Theory:
UV/Vis Spectrophotometry is the measurement of the absorption of monochromatic radiation by solutions of chemical substances in the range of 180nm to 380nm (uv) and 380 to 780nm (Vis.) of the spectrum respectively. According to the modified Beer-Lamberts equation
A = log10 1/T = log10 I0/I = a1%1cm * b * c Measurement of light absorption (in the form of absorbance) in the visible and ultraviolet range is widely used as a mean of identification or a supplementary evidence of identification. It is especially useful when the observed spectrum shows more than one characteristic maximum and when pH influences position of maxima. The ratio of the absorbances at two maxima also provide a useful criterion of identity. More over , in some cases, the absorbance at specified wavelength though may not maxima, is used as the basis of limit tests to exclude known or unknown impurities.
Procedure:
1)For Chlorpromazine hydrochloride identification and assay.
a)Dil. Hydrochloric acid( 50ml): Weigh accurately about 5.0g of Conc. Hydrochloric acid and dilute to 50ml with water.
b)Sample preparation: Each tablet claims to contain Chlorpromazine Hydrochloride = Weigh 20 tablets = Average weight of each tablet= Then pulverize the tablets.
i)For assay:(92.5-107.5)% of the stated amount of Chlorpromazine hydrochloride. Weigh accurately a quantity of the powder containing about 0.0625 g of Chlorpromazine Hydrochloride, add 5 ml of dilute hydrochloric acid and 100 ml of water. Shake for l5 minutes and add sufficient water to produce 250.0 ml. Centrifuge about 15 ml and to 5.0 ml of the clear, supernatant liquid add l0 ml of dilute hydrochloric acid and sufficient water to produce 250.0ml. Measure the absorbance of the resulting solution at the maximum at about 254nm. Calculate the content of Chlorpromazine hydrochloride taking 915 as the specific absorbance at 254nm. Wt.of sample taken=
ii)For identification: Examine the sample solution in the range 230nm to 360nm , it should show absorption maxima at about 254nm and 306 nm; absorbance at about 254nm should be 0.45 to 0.48.
Observation:
For identification absorption maxima recorded at: Absorbance recorded at 254nm: For assay, absorbance measured at 254nm: