Tank inventories

20000

18000

16000

14000

12000

Soft Water Tank Inventory

10000

WFI Tank Inventory

USP PW Tank Inventory

8000

6000

4000

2000

0
1.00

1.07

1.14

1.21

2.04

2.11

2.18

3.01

3.08

3.15

3.22

4.05

4.12

4.19

5.02

5.09

5.16

5.23

6.06

6.13

6.20

7.03

7.10

7.17

7000

6000

5000

4000

USP PW Makeup Rate

WFI Makeup Rate
Soft Water Makeup Rate

3000

2000

1000

1.00

1.07

1.14

1.21

2.04

2.11

2.18

3.01

3.08

3.15

3.22

4.05

4.12

4.19

5.02

5.09

5.16

5.23

6.06

6.13

6.20

7.03

7.10

7.17

Water Demands

7000

6000

5000

4000

WFI Demand
USP PW Demand

Soft Water Demand

3000

2000

1000

0
1

15

22

29

36

43

50

57

64

71

78

85

92

99

106

113

120

127

134

141

148

155

162

Purified Water Systems

Histograms: Typical
Uses

Histogram of Softened Water Usage and Break Tank Volume

Monday Time of Day (Hour of Day)

Sizing Assumptions
RO/DI skid drawoff

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24
445.63

445.63

445.63

445.63

445.63

534.18

3011.6

1858.6

796.89

3321.6

2836.4

2248

3030.6

2095.6

3030.6

1350.7

3030.6

2095.6

2815.6

445.63

3272.6

1864.6

445.63

445.63

HVAC: Humidification, non-cGMP areas

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

HVAC: Boiler makeup

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

2320.6

2320.6

2320.6

2320.6

2409.2

4886.6

3733.6

2671.9

5196.6

4711.4

4123

4905.6

3970.6

4905.6

3225.7

4905.6

3970.6

4690.6

2320.6

5147.6

3739.6

2320.6

2320.6

2320.6

1.00
15000
2320.6
L

1.01
15000
2320.6

1.02
15000
2320.6

1.03
15000
2320.6

1.04
15000
2409.2

1.05
15000
4886.6

1.06
15000
3733.6

1.07
15000
2671.9

1.08
15000
5196.6

1.09
15000
4711.4

1.1
15000
4123

1.11
15000
4905.6

1.12
15000
3970.6

1.13
15000
4905.6

1.14
15000
3225.7

1.15
15000
4905.6

1.16
15000
3970.6

1.17
15000
4690.6

1.18
15000
2320.6

1.19
15000
5147.6

1.2
15000
3739.6

1.21
15000
2320.6

1.22
15000
2320.6

1.23
15000
2320.6

(NOTE: RO/DI skid drawoff feeds USP PW tank. USP PW tank feeds WFI still,
clean steam generator, and CIP skids. Demands are not simultaneous.)

Total Soft Water Usage

Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume
Total weekly makeup

liter per hour

15000.0
100%
User Selectable
15000
7500
15000
504706.22

Average Daily Usage

Maximum Daily Usage
L
Lph
L

72101
85759

Daily Requirement,l
85759.0

Histogram of Softened Water Usage and Break Tank Volume

Tuesday Time of Day (Hour of Day)

Sizing Assumptions
RO/DI skid drawoff

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24
445.63

445.63

445.63

445.63

445.63

445.63

445.63

526.89

891.34

1112.9

3272.6

2095.6

3272.6

1665.5

3272.6

1874.7

4072.4

3393.6

2837.6

2141.3

545.25

445.63

2865.6

445.63

HVAC: Humidification, non-cGMP areas

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

HVAC: Boiler makeup

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2401.9

2766.3

2987.9

5147.6

3970.6

5147.6

3540.5

5147.6

3749.7

5947.4

5268.6

4712.6

4016.3

2420.2

2320.6

4740.6

2320.6

2320.6

2.00
15000
2320.6

2.01
15000
2320.6

2.02
15000
2320.6

2.03
15000
2320.6

2.04
15000
2320.6

2.05
15000
2320.6

2.06
15000
2401.9

2.07
15000
2766.3

2.08
15000
2987.9

2.09
15000
5147.6

2.10
15000
3970.6

2.11
15000
5147.6

2.12
15000
3540.5

2.13
15000
5147.6

2.14
15000
3749.7

2.15
15000
5947.4

2.16
15000
5268.6

2.17
15000
4712.6

2.18
15000
4016.3

2.19
15000
2420.2

2.20
15000
2320.6

2.21
15000
4740.6

2.22
15000
2320.6

2.23
15000
2320.6

(NOTE: RO/DI skid drawoff feeds USP PW tank. USP PW tank feeds WFI still,
clean steam generator, and CIP skids. Demands are not simultaneous.)

Total Soft Water Usage

Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume
Total weekly makeup

liter per hour

15000.0
100%
User Selectable
15000
7500
15000
504706.22

Daily Requirement,l
82851.3

Histogram of Softened Water Usage and Break Tank Volume

Wednesday Time of Day (Hour of Day)

Sizing Assumptions
RO/DI skid drawoff

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24
445.63

445.63

445.63

445.63

445.63

445.63

736.58

526.89

1041.3

1404.6

1871.7

2703.6

1021.5

2353.6

1457.7

3272.6

1873.6

641.34

445.63

445.63

445.63

445.63

445.63

445.63

HVAC: Humidification, non-cGMP areas

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

HVAC: Boiler makeup

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

2320.6

2320.6

2320.6

2320.6

2320.6

2611.6

2401.9

2916.3

3279.6

3746.7

4578.6

2896.5

4228.6

3332.7

5147.6

3748.6

2516.3

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

3.00
15000
2320.6

3.01
15000
2320.6

3.02
15000
2320.6

3.03
15000
2320.6

3.04
15000
2320.6

3.05
15000
2611.6

3.06
15000
2401.9

3.07
15000
2916.3

3.08
15000
3279.6

3.09
15000
3746.7

3.10
15000
4578.6

3.11
15000
2896.5

3.12
15000
4228.6

3.13
15000
3332.7

3.14
15000
5147.6

3.15
15000
3748.6

3.16
15000
2516.3

3.17
15000
2320.6

3.18
15000
2320.6

3.19
15000
2320.6

3.20
15000
2320.6

3.21
15000
2320.6

3.22
15000
2320.6

3.23
15000
2320.6

(NOTE: RO/DI skid drawoff feeds USP PW tank. USP PW tank feeds WFI still,
clean steam generator, and CIP skids. Demands are not simultaneous.)

Total Soft Water Usage

Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume
Total weekly makeup

liter per hour

15000.0
100%
User Selectable
15000
7500
15000
504706.22

Daily Requirement,l
69252.6

Histogram of Softened Water Usage and Break Tank Volume

Thursday Time of Day (Hour of Day)

Sizing Assumptions
RO/DI skid drawoff

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24
445.63

445.63

445.63

445.63

445.63

445.63

445.63

845.63

2582.6

595.63

1483.3

3272.6

955.33

4245.1

2386.6

3211.3

1552.6

2894.6

560.56

595.63

491.34

445.63

445.63

445.63

HVAC: Humidification, non-cGMP areas

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

HVAC: Boiler makeup

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

(NOTE: RO/DI skid drawoff feeds USP PW tank. USP PW tank feeds WFI still,
clean steam generator, and CIP skids. Demands are not simultaneous.)

380.4

Total Soft Water Usage

Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume
Total weekly makeup

liter per hour

15000.0
100%
User Selectable
15000
7500
15000
504706.22

380.4

380.4

380.4

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2720.6

4457.6

2470.6

3358.3

5147.6

2830.3

6120.1

4261.6

5086.3

3427.6

5150

2435.6

2851

2366.3

2701

2320.6

2701

2320.6

4.00
15000
2320.6

4.01
15000
2320.6

4.02
15000
2320.6

4.03
15000
2320.6

4.04
15000
2320.6

4.05
15000
2320.6

4.06
15000
2720.6

4.07
15000
4457.6

4.08
15000
2470.6

4.09
15000
3358.3

4.10
15000
5147.6

4.11
15000
2830.3

4.12
15000
6120.1

4.13
15000
4261.6

4.14
15000
5086.3

4.15
15000
3427.6

4.16
15000
5150

4.17
15000
2435.6

4.18
15000
2851

4.19
15000
2366.3

4.20
15000
2701

4.21
15000
2320.6

4.22
15000
2701

4.23
15000
2320.6

Daily Requirement,l
76650.7

Histogram of Softened Water Usage and Break Tank Volume

Friday Time of Day (Hour of Day)

Sizing Assumptions
RO/DI skid drawoff

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24
445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

1286.6

1585.2

2894.6

2837.6

3272.6

1746.5

3272.6

1760.2

3272.6

2095.6

1473.2

445.63

782.29

445.63

445.63

445.63

HVAC: Humidification, non-cGMP areas

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

HVAC: Boiler makeup

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

3161.6

3460.2

4769.6

4712.6

5147.6

3621.5

5147.6

3635.2

5147.6

3970.6

3348.2

2320.6

2657.3

2320.6

2320.6

2320.6

2320.6

5.00
15000
2320.6

5.01
15000
2320.6

5.02
15000
2320.6

5.03
15000
2320.6

5.04
15000
2320.6

5.05
15000
2320.6

5.06
15000
2320.6

5.07
15000
3161.6

5.08
15000
3460.2

5.09
15000
4769.6

5.10
15000
4712.6

5.11
15000
5147.6

5.12
15000
3621.5

5.13
15000
5147.6

5.14
15000
3635.2

5.15
15000
5147.6

5.16
15000
3970.6

5.17
15000
3348.2

5.18
15000
2320.6

5.19
15000
2657.3

5.20
15000
2320.6

5.21
15000
2320.6

5.22
15000
2320.6

5.23
15000
2320.6

(NOTE: RO/DI skid drawoff feeds USP PW tank. USP PW tank feeds WFI still,
clean steam generator, and CIP skids. Demands are not simultaneous.)

Total Soft Water Usage

Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume
Total weekly makeup

liter per hour

15000.0
100%
User Selectable
15000
7500
15000
504706.22

Daily Requirement,l
76627.3

Histogram of Softened Water Usage and Break Tank Volume

Saturday Time of Day (Hour of Day)

Sizing Assumptions
RO/DI skid drawoff

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24
445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

634.88

445.63

445.63

445.63

445.63

2078.6

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

HVAC: Humidification, non-cGMP areas

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

HVAC: Boiler makeup

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2509.9

2320.6

2320.6

2320.6

2320.6

3953.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

6.00
15000
2320.6

6.01
15000
2320.6

6.02
15000
2320.6

6.03
15000
2320.6

6.04
15000
2320.6

6.05
15000
2320.6

6.06
15000
2320.6

6.07
15000
2509.9

6.08
15000
2320.6

6.09
15000
2320.6

6.10
15000
2320.6

6.11
15000
2320.6

6.12
15000
3953.6

6.13
15000
2320.6

6.14
15000
2320.6

6.15
15000
2320.6

6.16
15000
2320.6

6.17
15000
2320.6

6.18
15000
2320.6

6.19
15000
2320.6

6.20
15000
2320.6

6.21
15000
2320.6

6.22
15000
2320.6

6.23
15000
2320.6

(NOTE: RO/DI skid drawoff feeds USP PW tank. USP PW tank feeds WFI still,
clean steam generator, and CIP skids. Demands are not simultaneous.)

Total Soft Water Usage

Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume
Total weekly makeup

liter per hour

15000.0
100%
User Selectable
15000
7500
15000
504706.22

Daily Requirement,l
57517.3

Histogram of Softened Water Usage and Break Tank Volume

Sunday Time of Day (Hour of Day)

Sizing Assumptions
RO/DI skid drawoff

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24
445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

445.63

HVAC: Humidification, non-cGMP areas

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

358

HVAC: Boiler makeup

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

1517

(NOTE: RO/DI skid drawoff feeds USP PW tank. USP PW tank feeds WFI still,
clean steam generator, and CIP skids. Demands are not simultaneous.)

353.1

Total Soft Water Usage

Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume
Total weekly makeup

liter per hour

15000.0
100%
User Selectable
15000
7500
15000
504706.22

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2673.7

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

2320.6

7.00
15000
2320.6

7.01
15000
2320.6

7.02
15000
2320.6

7.03
15000
2320.6

7.04
15000
2320.6

7.05
15000
2320.6

7.06
15000
2320.6

7.07
15000
2320.6

7.08
15000
2320.6

7.09
15000
2320.6

7.10
15000
2320.6

7.11
15000
2320.6

7.12
15000
2320.6

7.13
15000
2320.6

7.14
15000
2320.6

7.15
15000
2320.6

7.16
15000
2673.7

7.17
15000
2320.6

7.18
15000
2320.6

7.19
15000
2320.6

7.20
15000
2320.6

7.21
15000
2320.6

7.22
15000
2320.6

7.23
15000
2320.6

Daily Requirement,l
56048.1

Soft Water Tank Volume and Makeup

Tank Volume

10000

USP PW Makeup

18000

16500

9000

15000
8000
13500
7000

6000

10500

5000

9000

7500

4000

6000
3000

4500
2000
3000

1000

1500

Time (Day. Hour)

Tank Volume, Liters

System Makeup, LPH

12000

Histogram of USP Water Usage

System Makeup and Tank Volume

Monday Time of Day (Hour of Day)

Sizing Assumptions

WFI feed
Clean steam generation
CIP skid, suite 1 (all except final rinse)

Total USP Purified Water Usage

Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

1413

1413

270

1650

1650

1650

1650

1650

1650

614.109

1650

1650

1435.02

1650

830

445.625 445.625 445.625 445.625 534.175 445.625 445.625 526.889 736.575 597.425 597.994 445.625 445.625 445.625 736.575 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625
2149

liter per hour

1534.0
51%
User Selectable
3000
3000
3000

1153

935

589

935

935

935

935

1177

589

445.625 445.625 445.625 445.625 534.175 3011.63 1858.63 796.889 3321.58 2836.43 2247.99 3030.63 2095.63 3030.63 1350.68 3030.63 2095.63 2815.65 445.625 3272.63 1864.63 445.625 445.625 445.625
1.00
1.01
1.02
1.03
1.04
3000
3000
3000
3000
3000
445.625 445.625 445.625 445.625 534.175
L

1.05
3000
3000

1.06
1.07
2988.38 3000
1870.25 796.889

1.08
3000
3000

1.09
1.1
2678.43 2842
3000 2405.99

1.11
3000
3000

1.12
2969.38
2126.25

1.13
3000
3000

1.14
2969.38
1381.31

1.15
3000
3000

1.16
1.17
1.18
2969.38 3000
3000
2126.25 2815.65 445.625

1.19
3000
3000

1.2
1.21
1.22
1.23
2727.38 3000
3000
3000
2137.25 445.625 445.625 445.625
Daily Requirement,l

L
Lph
L

Average Daily Usage

Maximum Daily Usage
Maximum 2-day Usage
Total Usage for Week

26833.1
40759
78610.3
187832

40759

Histogram of USP Water Usage

System Makeup and Tank Volume

Tuesday Time of Day (Hour of Day)

Sizing Assumptions

WFI feed
Clean steam generation
CIP skid, suite 1 (all except final rinse)

Total USP Purified Water Usage

Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

400

330.587

1650

1650

1650

1219.86

1650

1429.08

1650

1650

1650

1650

99.6207

1413

445.625 445.625 445.625 445.625 445.625 445.625 526.889 491.336 782.286 445.625 445.625 445.625 445.625 445.625 445.625 445.625 736.575 445.625 491.336 445.625 445.625 445.625 445.625 445.625
2149

liter per hour

1177

User Selectable
3000
3000
3000

1177

1976.82

1007

742

1007

445.625 445.625 445.625 445.625 445.625 445.625 526.889 891.336 1112.87 3272.63 2095.63 3272.63 1665.48 3272.63 1874.71 4072.44 3393.58 2837.63 2141.34 545.246 445.625 2865.63 445.625 445.625
2.00
2.01
2.02
2.03
2.04
2.05
2.06
2.07
2.08
3000
3000
3000
3000
3000
3000
3000
3000
3000
445.625 445.625 445.625 445.625 445.625 445.625 526.889 891.336 1112.87

1534.0
51%

1177

2.09
3000
3000

2.10
2727.38
2368.25

2.11
3000
3000

2.12
2727.38
1938.11

2.13
3000
3000

2.14
2727.38
2147.33

2.15
3000
3000

2.16
2.17
2.18
2.19
2.20
2.21
2.22
2.23
1927.56 1533.98 1696.36 2555.02 3000
3000
3000
3000
3000
3000
3000 990.223 445.625 2865.63 445.625 445.625
Daily Requirement,l

37851.3

Histogram of USP Water Usage

System Makeup and Tank Volume

Wednesday Time of Day (Hour of Day)

Sizing Assumptions

WFI feed
Clean steam generation
CIP skid, suite 1 (all except final rinse)

Total USP Purified Water Usage

Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

550

959

1426.03

1459

284.952

1109

1012.04

1650

1428

150

445.625 445.625 445.625 445.625 445.625 736.575 526.889 491.336 445.625 445.625 445.625 736.575 445.625 445.625 445.625 445.625 491.336 445.625 445.625 445.625 445.625 445.625 445.625 445.625
2149

liter per hour

799

799

445.625 445.625 445.625 445.625 445.625 736.575 526.889 1041.34 1404.63 1871.66 2703.63 1021.53 2353.63 1457.66 3272.63 1873.63 641.336 445.625 445.625 445.625 445.625 445.625 445.625 445.625
3.00
3.01
3.02
3.03
3.04
3.05
3.06
3.07
3.08
3.09
3.10
3.11
3.12
3.13
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
445.625 445.625 445.625 445.625 445.625 736.575 526.889 1041.34 1404.63 1871.66 2703.63 1021.53 2353.63 1457.66

1534.0
51%
User Selectable
3000
3000
3000

1177

3.14
3000
3000

3.15
3.16
3.17
3.18
3.19
3.20
3.21
3.22
3.23
2727.38 3000
3000
3000
3000
3000
3000
3000
3000
2146.25 641.336 445.625 445.625 445.625 445.625 445.625 445.625 445.625
Daily Requirement,l

24252.6

Histogram of USP Water Usage

System Makeup and Tank Volume

Thursday Time of Day (Hour of Day)

Sizing Assumptions

WFI feed
Clean steam generation
CIP skid, suite 1 (all except final rinse)

Total USP Purified Water Usage

Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume

10

11

12

13

14

400

1166

150

746.703

1650

509.703

1650

1650

15

16

1588.7 1107.02

17

18

19

20

21

22

23

24

1650

114.932

150

445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 736.575 445.625 445.625 445.625 736.575 445.625 445.625 445.625 445.625 445.625 491.336 445.625 445.625 445.625 445.625
2149

liter per hour

971

User Selectable
3000
3000
3000

2149.43

1177

799

445.625 445.625 445.625 445.625 445.625 445.625 845.625 2582.63 595.625 1483.28 3272.63 955.328 4245.05 2386.58 3211.33 1552.65 2894.63 560.557 595.625 491.336 445.625 445.625 445.625 445.625
4.00
4.01
4.02
4.03
4.04
4.05
4.06
4.07
4.08
4.09
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
445.625 445.625 445.625 445.625 445.625 445.625 845.625 2582.63 595.625 1483.28

1534.0
51%

1177

4.10
3000
3000

4.11
2727.38
1227.95

4.12
3000
3000

4.13
4.14
4.15
4.16
4.17
4.18
4.19
4.20
4.21
4.22
4.23
1754.95 2368.37 2157.05 3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
2395.6 2894.63 560.557 595.625 491.336 445.625 445.625 445.625 445.625
Daily Requirement,l

30129.1

Histogram of USP Water Usage

System Makeup and Tank Volume

Friday Time of Day (Hour of Day)

Sizing Assumptions

WFI feed
Clean steam generation
CIP skid, suite 1 (all except final rinse)

Total USP Purified Water Usage

Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

550

1139.59

1650

1650

1650

1300.86

1650

1314.62

1650

1650

1027.62

445.625 445.625 445.625 445.625 445.625 445.625 445.625 736.575 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 782.286 445.625 445.625 445.625 445.625
2149

liter per hour

799

742

User Selectable
3000
3000
3000

1177

1177

445.625 445.625 445.625 445.625 445.625 445.625 445.625 1286.58 1585.21 2894.63 2837.63 3272.63 1746.48 3272.63 1760.24 3272.63 2095.63 1473.25 445.625 782.286 445.625 445.625 445.625 445.625
5.00
5.01
5.02
5.03
5.04
5.05
5.06
5.07
5.08
5.09
5.10
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
445.625 445.625 445.625 445.625 445.625 445.625 445.625 1286.58 1585.21 2894.63 2837.63

1534.0
51%

1177

5.11
3000
3000

5.12
2727.38
2019.11

5.13
3000
3000

5.14
2727.38
2032.87

5.15
3000
3000

5.16
5.17
5.18
5.19
5.20
5.21
5.22
5.23
2727.38 3000
3000
3000
3000
3000
3000
3000
2368.25 1473.25 445.625 782.286 445.625 445.625 445.625 445.625
Daily Requirement,l

31627.3

Histogram of USP Water Usage

System Makeup and Tank Volume

Saturday Time of Day (Hour of Day)

Sizing Assumptions

WFI feed
Clean steam generation
CIP skid, suite 1 (all except final rinse)

Total USP Purified Water Usage

Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

189.25

891

445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625
2149

liter per hour

742

445.625 445.625 445.625 445.625 445.625 445.625 445.625 634.875 445.625 445.625 445.625 445.625 2078.63 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625
6.00
6.01
6.02
6.03
6.04
6.05
6.06
6.07
6.08
6.09
6.10
6.11
6.12
6.13
6.14
6.15
6.16
6.17
6.18
6.19
6.20
6.21
6.22
6.23
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
445.625 445.625 445.625 445.625 445.625 445.625 445.625 634.875 445.625 445.625 445.625 445.625 2078.63 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625

1534.0
51%
User Selectable
3000
3000
3000

Daily Requirement,l

12517.3

Histogram of USP Water Usage

System Makeup and Tank Volume

Sunday Time of Day (Hour of Day)

Sizing Assumptions

WFI feed
Clean steam generation
CIP skid, suite 1 (all except final rinse)

Total USP Purified Water Usage

Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625
2149

liter per hour

445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625
7.00
7.01
7.02
7.03
7.04
7.05
7.06
7.07
7.08
7.09
7.10
7.11
7.12
7.13
7.14
7.15
7.16
7.17
7.18
7.19
7.20
7.21
7.22
7.23
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
3000
445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625 445.625

1534.0
51%
User Selectable
3000
3000
3000

Daily Requirement,l

10695

Time (Day. Hour)

7.21

7.18

7.15

7.12

7.09

7.06

7.03

7.00

6.21

6.18

Tank Volume, Liters

Tank Volume

6.15

6.12

6.09

6.06

6.03

6.00

5.21

5.18

5.15

5.12

5.09

5.06

5.03

5.00

4.21

4.18

4.15

4.12

4.09

4.06

4.03

4.00

3.21

3.18

3.15

3.12

3.09

3.06

3.03

3.00

2.21

2.18

2.15

2.12

2.09

2.06

2.03

2.00

1.21

1.18

1.15

1.12

1.09

1.06

1.03

1.00

System Makeup, LPH

USP PW Tank Volume and Makeup

USP PW Makeup

7500
7500

6000
6000

4500
4500

3000
3000

1500
1500

0
0

Histogram of Water for Injection Usage

System Makeup and Tank Volume

Monday Time of Day (Hour of Day)

Sizing Assumptions

1500L Mammalian Purification Process Train #1 Starts on Monday

CIP BR-1070

10

11

12

CEN-1110
CIP

C'fuge(CEN-1110) Seal

13

14

15

FL-1120 CIP

16

17

18

19

20
UF-1140 CIP

UF-113006B/FL-113007B

21

22

23

24

FL-1150 CIP

1500 L Mammalian Media Prep

TK-1560
[800L BP TK]
CIP

1500 L Mammalian Buffer Prep

Use points
(max. consumption)
Mam Suite 1, Cell Culture
Mam Suite 1, V+ Purification
Mam Suite 1, V- Purification
Suite 1 Media Preparation
Suite 1 Buffer Preparation
Suite 1 Washer

TK-1560
[800L BP TK]
CIP
B#2 Prep

TK-1560
[800L BP TK]
CIP
B#3 Prep

Suite 1
Washer

Suite 1 Washer
Fill/finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP
Fill / finish: Lyophilizer Wash-Out
1780.00
1413.00
1780.00
1166.00
0.00
1413.00
400.00
#REF!
#REF!
150.00
500.00
500.00
706.00
200.00

B#1 Prep

TK-1560
[800L BP TK]
CIP
B#4 Prep

TK-1530
[250L BP TK]
CIP

Suite 1
Washer

Dirty PW

Dirty PW
Wash Vials

Dirty PW
Wash Vials

Clean PW
200L Tk WFI Rinse

25 lpm max per drop

25 lpm max per drop
25 lpm max per drop
25 lpm max per drop
25 lpm max per drop

1413.0

200L Tk WFI Rinse

1413.0
120.0

120.0

700.0

1413.0

1000.0

246.7

1413.0

246.7

1413.0

246.7

400.0

Utility area: COP

Fill / finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP (WFI Rinse Only)
Fill / finish: Lyophilizer Wash-Out

959 l per run

150 l per run
500 l per run
500 l per run
706 l per run
200 l per run

Total WFI Water Usage

liter per hour

959.0

1413.0
400.0

148.0

1780.0

700.0

959.0

959.0

150.0

150.0
500.0

150.0
500.0

500.0
706.0

706.0

0.00

Time (Day. Hour)

Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume

463.0 (time-averaged)
1250.4
42%
User Selectable
3000
1650
3000

1413

1413

270

2492

2306

746.7

1413

1396.7

1913

246.7

2772

1004

959

1780

700

1.00
3000
0
L

1.01
3000
0

1.02
3000
0

1.03
3000
0

1.04
3000
0

1.05
3000
1413

1.06
3000
1413

1.07
3000
270

1.08
3000
1650

1.09
2158
1650

1.1
1502
1650

1.11
2405.3
1650

1.12
2642.3
1650

1.13
2895.6
1650

1.14
2632.6
614.11

1.15
3000
1650

1.16
1878
1650

1.17
2524
1435

1.18
3000
0

1.19
3000
1650

1.2
2870
830

1.21
3000
0

1.22
3000
0

1.23
3000
0

L
Lph
L

Daily Average 11113

Daily Maximum 20825
2-day Maximum 38917

Note:
The makeup rate is the WFI Still product flowrate.
WFI requirement for CIP is 12 minutes of flow at 37.3 liters/minute-m of circumference.

liters
liters
liters

Daily Requirement,l
Idle, hrs
PRODUCTION Max, hrs
Avg, hrs

20825
9
0
15

Histogram of Water for Injection Usage

System Makeup and Tank Volume

Tuesday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

1500L Mammalian Purification Process Train #1 Starts on Monday

14

15

16

17

Protein A LC-1180 Push

T-1190 CIP

TK-1560
[800L BP TK]
CIP
B#7 Prep

TK-1560
[800L BP TK]
CIP
B#8 Prep

18

19

20

21

22

23

24

UF-1220 CIP
(Future)

T-1200 CIP

1500 L Mammalian Media Prep

1500 L Mammalian Buffer Prep

B#5 Prep

Use points
(max. consumption)
Mam Suite 1, Cell Culture
Mam Suite 1, V+ Purification
Mam Suite 1, V- Purification
Suite 1 Media Preparation
Suite 1 Buffer Preparation
Suite 1 Washer

TK-1560
[800L BP TK]
CIP
B#7 Prep

TK-1530
[250L BP TK]
CIP

Suite 1
washer

Suite 1 Washer
Fill/finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP
Fill / finish: Lyophilizer Wash-Out
1780.00
1413.00
1780.00
1166.00
0.00
1413.00
400.00
#REF!
#REF!
150.00
500.00
500.00
706.00
200.00

TK-1530
[250L BP TK]
CIP
B#6Prep

Dirty PW

Dirty PW
Wash Vials

Dirty PW

Wash Vials
Clean PW
200L Tk WFI Rinse

25 lpm max per drop

25 lpm max per drop
25 lpm max per drop
25 lpm max per drop
25 lpm max per drop

180.6

959.0

281.2

200L Tk WFI Rinse

1413.0

851.6

82.2

82.2

959.0

1413.0

1413.0

851.6

1413.0

140.6

1413.0

959.0

400.0

Utility area: COP

Fill / finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP (WFI Rinse Only)
Fill / finish: Lyophilizer Wash-Out

959 l per run

150 l per run
500 l per run
500 l per run
706 l per run
200 l per run

Total WFI Water Usage

liter per hour

959.0

959.0

150.0

150.0
500.0

500.0

150.0

500.0
706.0

706.0

0.00

Time (Day. Hour)

Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume

463.0 (time-averaged)
1250.4
42%
User Selectable
3000
1650
3000

400

330.59

2418

1487.2

1413

851.62

2145.2

933.85

2372

2512.6

1815

1413

2.00
3000
0

2.01
3000
0

2.02
3000
0

2.03
3000
0

2.04
3000
0

2.05
3000
0

2.06
3000
0

2.07
3000
400

2.08
3000
330.59

2.09
3000
1650

2.10
2232
1650

2.11
2394.8
1650

2.12
2631.8
1219.9

2.13
3000
1650

2.14
2504.8
1429.1

2.15
3000
1650

2.16
2278
1650

2.17
1415.4
1650

2.18
1250.4
1650

2.19
2900.4
99.621

2.20
3000
0

2.21
3000
1413

2.22
3000
0

2.23
3000
0

Daily Requirement,l
Idle, hrs
PRODUCTION Max, hrs
Avg, hrs

18092
10
0
14

Histogram of Water for Injection Usage

System Makeup and Tank Volume

Wednesday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

SP-S LC-1230
WFI Push
UF-1240 Viresolve (Operation)

1500L Mammalian Purification Process Train #1 Starts on Monday

1500 L Mammalian Media Prep
1500 L Mammalian Buffer Prep

B#9 Prep

Use points
(max. consumption)
Mam Suite 1, Cell Culture
Mam Suite 1, V+ Purification
Mam Suite 1, V- Purification
Suite 1 Media Preparation
Suite 1 Buffer Preparation
Suite 1 Washer

TK-1530
[250L BP TK]
CIP
B#13Prep

TK-1560
[800L BP TK]
CIP

Suite 1
washer

Suite 1 Washer
Fill/finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP
Fill / finish: Lyophilizer Wash-Out
1780.00
1413.00
1780.00
1166.00
0.00
1413.00
400.00
#REF!
#REF!
150.00
500.00
500.00
706.00
200.00

TK-1530
[250L BP TK]
CIP
B#10Prep

Dirty PW

Dirty PW
Wash Vials

Dirty PW
Wash Vials

Clean PW
200L Tk WFI Rinse

25 lpm max per drop

25 lpm max per drop
25 lpm max per drop
25 lpm max per drop
25 lpm max per drop

200L Tk WFI Rinse

140.6

220.0

959.0

144.3

959.0

512.0

1413.0

400.0

Utility area: COP

Fill / finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP (WFI Rinse Only)
Fill / finish: Lyophilizer Wash-Out

959 l per run

150 l per run
500 l per run
500 l per run
706 l per run
200 l per run

Total WFI Water Usage

liter per hour

959.0

959.0

150.0

150.0
500.0

150.0
500.0

500.0
706.0

706.0

0.00

Time (Day. Hour)

Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume

463.0 (time-averaged)
1250.4
42%
User Selectable
3000
1650
3000

550

959

1426

1459

284.95

1109

1012

2372

706

150

3.00
3000
0

3.01
3000
0

3.02
3000
0

3.03
3000
0

3.04
3000
0

3.05
3000
0

3.06
3000
0

3.07
3000
550

3.08
3000
959

3.09
3000
1426

3.10
3000
1459

3.11
3000
284.95

3.12
3000
1109

3.13
3000
1012

3.14
3000
1650

3.15
2278
1428

3.16
3000
150

3.17
3000
0

3.18
3000
0

3.19
3000
0

3.20
3000
0

3.21
3000
0

3.22
3000
0

3.23
3000
0

Daily Requirement,l
Idle, hrs
PRODUCTION Max, hrs
Avg, hrs

10028
14
0
10

Histogram of Water for Injection Usage

System Makeup and Tank Volume

Thursday Time of Day (Hour of Day)

Sizing Assumptions

1500L Mammalian Purification Process Train #1 Starts on Monday

8
UF-1240 CIP

10

11

12

13

14

15

16

17

UF-1270 CIP

UF-1270 (Operation)

18

19

20

21

22

23

24

IEC LC-1300
WFI Push

1500 L Mammalian Media Prep

1500 L Mammalian Buffer Prep

B#1 Prep

Use points
(max. consumption)
Mam Suite 1, Cell Culture
Mam Suite 1, V+ Purification
Mam Suite 1, V- Purification
Suite 1 Media Preparation
Suite 1 Buffer Preparation
Suite 1 Washer

TK-1560
[800L BP TK]
CIP

B#3 Prep

TK-1560
[800L BP TK]
CIP

B#4 Prep

TK-1530
[250L BP TK]
CIP

Suite 1
washer

Suite 1 Washer
Fill/finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP
Fill / finish: Lyophilizer Wash-Out
1780.00
1413.00
1780.00
1166.00
0.00
1413.00
400.00
#REF!
#REF!
150.00
500.00
500.00
706.00
200.00

TK-1560
[800L BP TK]
CIP
B#2 Prep

Dirty PW

Dirty PW
Wash Vials

Dirty PW

Wash Vials
Clean PW
200L Tk WFI Rinse

25 lpm max per drop

25 lpm max per drop
25 lpm max per drop
25 lpm max per drop
25 lpm max per drop

1166.0
1166.0
246.7

1413.0

246.7

1413.0

99.9
246.7

1413.0

148.0

959.0

400.0

Utility area: COP

Fill / finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP (WFI Rinse Only)
Fill / finish: Lyophilizer Wash-Out

959 l per run

150 l per run
500 l per run
500 l per run
706 l per run
200 l per run

Total WFI Water Usage

liter per hour

959.0
150.0

150.0
500.0

500.0

150.0

500.0
706.0

0.00

Time (Day. Hour)

Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume

463.0 (time-averaged)
1250.4
42%
User Selectable
3000
1650
3000

400

1166

150

746.7

1913

246.7

2579

896.7

1413

1107

1665

99.932

150

4.00
3000
0

4.01
3000
0

4.02
3000
0

4.03
3000
0

4.04
3000
0

4.05
3000
0

4.06
3000
400

4.07
3000
1166

4.08
3000
150

4.09
3000
746.7

4.10
3000
1650

4.11
2737
509.7

4.12
3000
1650

4.13
2071
1650

4.14
2824.3
1588.7

4.15
3000
1107

4.16
3000
1650

4.17
2985
114.93

4.18
3000
150

4.19
3000
0

4.20
3000
0

4.21
3000
0

4.22
3000
0

4.23
3000
0

Daily Requirement,l
Idle, hrs
PRODUCTION Max, hrs
Avg, hrs

12533
11
0
13

Histogram of Water for Injection Usage

System Makeup and Tank Volume

Friday Time of Day (Hour of Day)

Sizing Assumptions

1500L Mammalian Purification Process Train #1 Starts on Monday

10

UF-1310

11

12

13

14

15

16

17

18

TK-1560
[800L BP TK]
CIP

B#7a Prep

TK-1560
[800L BP TK]
CIP

B#7b Prep

TK-1560
[800L BP TK]
CIP

B#8 Prep

TK-1530
[250L BP TK]
CIP

19

20

21

22

23

24

UF-1310 CIP

1500 L Mammalian Media Prep

1500 L Mammalian Buffer Prep

B#5 Prep
Suite 1
washer

Suite 1 Washer
Fill/finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP
Fill / finish: Lyophilizer Wash-Out
1780.00
1413.00
1780.00
1166.00
0.00
1413.00
400.00
#REF!
#REF!
150.00
500.00
500.00
706.00
200.00

Use points
(max. consumption)
Mam Suite 1, Cell Culture
Mam Suite 1, V+ Purification
Mam Suite 1, V- Purification
Suite 1 Media Preparation
Suite 1 Buffer Preparation
Suite 1 Washer

TK-1530
[250L BP TK]
CIP
B#6Prep

Dirty PW

Dirty PW
Wash Vials

Dirty PW
Wash Vials

Clean PW
200L Tk WFI Rinse

200L Tk WFI Rinse

Lyo Washout

25 lpm max per drop

25 lpm max per drop
25 lpm max per drop
25 lpm max per drop
25 lpm max per drop

890.0
180.6

959.0

281.2

1413.0

851.6

1413.0

851.6

1413.0

140.6

959.0

400.0

Utility area: COP

Fill / finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP (WFI Rinse Only)
Fill / finish: Lyophilizer Wash-Out

959 l per run

150 l per run
500 l per run
500 l per run
706 l per run
200 l per run

Total WFI Water Usage

liter per hour

959.0

959.0

150.0

150.0
500.0

150.0
500.0

500.0
706.0

706.0
200.0

0.00

Time (Day. Hour)

Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume

463.0 (time-averaged)
1250.4
42%
User Selectable
3000
1650
3000

550

1139.6

2165

1671.2

1413

1001.6

1913

1051.6

2372

996.62

959

5.00
3000
0

5.01
3000
0

5.02
3000
0

5.03
3000
0

5.04
3000
0

5.05
3000
0

5.06
3000
0

5.07
3000
550

5.08
3000
1139.6

5.09
3000
1650

5.10
2485
1650

5.11
2463.8
1650

5.12
2700.8
1300.9

5.13
3000
1650

5.14
2737
1314.6

5.15
3000
1650

5.16
2278
1650

5.17
2931.4
1027.6

5.18
3000
0

5.19
3000
0

5.20
3000
0

5.21
3000
0

5.22
3000
0

5.23
3000
0

Daily Requirement,l
Idle, hrs
PRODUCTION Max, hrs
Avg, hrs

15233
13
0
11

Histogram of Water for Injection Usage

System Makeup and Tank Volume

Saturday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

SEC LC-1320
WFI Push
UF-1310 (Operation)

1500L Mammalian Purification Process Train #1 Starts on Monday

13

14

15

16

17

18

19

20

21

22

23

24

UF-1310 CIP

1500 L Mammalian Media Prep

1500 L Mammalian Buffer Prep
Suite 1 Washer
Fill/finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP
Fill / finish: Lyophilizer Wash-Out
1780.00
1413.00
1780.00
1166.00
0.00
1413.00
400.00
#REF!
#REF!
150.00
500.00
500.00
706.00
200.00

Use points
(max. consumption)
Mam Suite 1, Cell Culture
Mam Suite 1, V+ Purification
Mam Suite 1, V- Purification
Suite 1 Media Preparation
Suite 1 Buffer Preparation
Suite 1 Washer

25 lpm max per drop

25 lpm max per drop
25 lpm max per drop
25 lpm max per drop
25 lpm max per drop

Utility area: COP

Fill / finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP (WFI Rinse Only)
Fill / finish: Lyophilizer Wash-Out

959 l per run

150 l per run
500 l per run
500 l per run
706 l per run
200 l per run

Total WFI Water Usage

liter per hour

189.3

891.0

0.00

Time (Day. Hour)

Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume

463.0 (time-averaged)
1250.4
42%
User Selectable
3000
1650
3000

189.25

891

6.00
3000
0

6.01
3000
0

6.02
3000
0

6.03
3000
0

6.04
3000
0

6.05
3000
0

6.06
3000
0

6.07
3000
189.25

6.08
3000
0

6.09
3000
0

6.10
3000
0

6.11
3000
0

6.12
3000
891

6.13
3000
0

6.14
3000
0

6.15
3000
0

6.16
3000
0

6.17
3000
0

6.18
3000
0

6.19
3000
0

6.20
3000
0

6.21
3000
0

6.22
3000
0

6.23
3000
0

Daily Requirement,l
Idle, hrs
PRODUCTION Max, hrs
Avg, hrs

1080.3
22
0
2

Histogram of Water for Injection Usage

System Makeup and Tank Volume

Sunday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

7.00
3000
0

7.01
3000
0

7.02
3000
0

7.03
3000
0

7.04
3000
0

7.05
3000
0

7.06
3000
0

7.07
3000
0

7.08
3000
0

7.09
3000
0

7.10
3000
0

7.11
3000
0

7.12
3000
0

7.13
3000
0

7.14
3000
0

7.15
3000
0

7.16
3000
0

7.17
3000
0

7.18
3000
0

7.19
3000
0

7.20
3000
0

7.21
3000
0

7.22
3000
0

7.23
3000
0

1500L Mammalian Purification Process Train #1 Starts on Monday

1500 L Mammalian Media Prep
1500 L Mammalian Buffer Prep
Suite 1 Washer
Fill/finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP
Fill / finish: Lyophilizer Wash-Out
1780.00
1413.00
1780.00
1166.00
0.00
1413.00
400.00
#REF!
#REF!
150.00
500.00
500.00
706.00
200.00

Use points
(max. consumption)
Mam Suite 1, Cell Culture
Mam Suite 1, V+ Purification
Mam Suite 1, V- Purification
Suite 1 Media Preparation
Suite 1 Buffer Preparation
Suite 1 Washer

25 lpm max per drop

25 lpm max per drop
25 lpm max per drop
25 lpm max per drop
25 lpm max per drop

Utility area: COP

Fill / finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP (WFI Rinse Only)
Fill / finish: Lyophilizer Wash-Out

959 l per run

150 l per run
500 l per run
500 l per run
706 l per run
200 l per run

Total WFI Water Usage

liter per hour

0.00

Time (Day. Hour)

Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory:

Initial Tank Volume

Make-Up Rate
Tank Working Volume

463.0 (time-averaged)
1250.4
42%
User Selectable
3000
1650
3000

Daily Requirement,l
Idle, hrs
PRODUCTION Max, hrs
Avg, hrs

0
24
0
0

Water for Injection System:

System Make-Up and Tank Volume
1800

Tank Volume

WFI Makeup

4000

1650
3500
1500

1350

3000

1200

1050

900

2000

750

1500
600

450

1000

300
500
150

Time (Day. Hour)

Tank Volume, Liters

System Makeup, LPH

2500

Histogram of Pure Steam Usage

Monday Time of Day (Hour of Day)

Sizing Assumptions

SIP CEN113001B

1500L Mammalian Purification Process Suite 1 Starts on Monday

Fuge(CEN-113001B)

10

11

SIP FL113004B

SIP UF113006B

12

13

14

15

16

17

18

19

20

21

22

23

24

UF-113006B/FL-113007B

Suite 1 Media Prep

Suite 1 Buffer Prep

SIP 800L BPT

SIP BR112011A

500 L Mammalian Cell Culture Process Suite 2 Starts on Monday

Use points
(max. consumption)
Building 1C
M1-CC-1 Mam Suite 1, Cell Culture
M1-P1 Mam Suite 1, V+ Purification
M1-P2 Mam Suite 1, V- Purification
Mam Suite 1 Buffer Preparation
FF-Auto Filling Suite Autoclave
End user load excluding HVAC

800L BP TK
CIP

B#1 Prep

600L BP TK
CIP

B#2 Prep

600L BP TK
CIP

B#3 Prep

600L BP TK
CIP

B#4 Prep

600L BP TK
CIP

START BR-112011A (500l) RUN

Rooms

SIP: 0.0803 kg/liter

SIP: 0.0803 kg/liter
SIP: 0.0803 kg/liter
SIP: 0.0803 kg/liter
230 kg/load

70

120

120.45

120

120.45

64.24
0

70

64.24

230
230

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

10%

35.227

35.227

35.227

35.227

42.227

35.227

35.227

41.651

58.227

47.227

47.272

35.227

35.227

35.227

58.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

Total Pure Steam Usage

USP Water Supply, LPH
Time (Day. Hour)
Time-averaged production

kg/hr
782.285775

387.5
445.63
1.00

387.5
445.63
1.01

387.5
445.63
1.02

387.5
445.63
1.03

464.5
534.18
1.04

387.5
445.63
1.05

387.5
445.63
1.06

458.16
526.89
1.07

640.5
736.58
1.08

519.5
597.43
1.09

520
597.99
1.1

387.5
445.63
1.11

387.5
445.63
1.12

387.5
445.63
1.13

640.5
736.58
1.14

387.5
445.63
1.15

387.5
445.63
1.16

387.5
445.63
1.17

387.5
445.63
1.18

387.5
445.63
1.19

387.5
445.63
1.2

387.5
445.63
1.21

387.5
445.63
1.22

387.5
445.63
1.23

Maximum Pure Steam Load

Line loss, maximum
Blowdown
Safety factor
Pure steam generator
Pure Steam Pressure
Blowdown percentage
Minimum load

680
68.0
102.0
20%
1100
2.4
15%
35%

AHU (humidification)
Line Loss

230
230

407.5
kg/hr

kg/hr
bar

Idle, hrs
Max, hrs

0
4

Avg, hrs

20

Daily use less humidification

Daily use, total
Daily line loss

1763.6
10218
928.9

Histogram of Pure Steam Usage

Tuesday Time of Day (Hour of Day)

Sizing Assumptions

1500L Mammalian Purification Process Suite 1 Starts on Monday

SIP T-115002

10

11

12

13

SIP T-115003

14

15

16

17

18

19

20

21

22

23

24

SIP UF115011
(Future)

Protein A LPC-115001 Push

Suite 1 Media Prep

Suite 1 Buffer Prep

SIP 800L BPT

B#5 Prep

800L BP TK
CIP

B#6Prep

800L BP TK
CIP

B#7a Prep

800L BP TK
CIP

B#7b Prep

2000L BP TK
CIP

B#8 Prep

BP TK CIP

500 L Mammalian Cell Culture Process Suite 2 Starts on Monday

Use points
(max. consumption)
Building 1C
M1-CC-1 Mam Suite 1, Cell Culture
M1-P1 Mam Suite 1, V+ Purification
M1-P2 Mam Suite 1, V- Purification
Mam Suite 1 Buffer Preparation
FF-Auto Filling Suite Autoclave
End user load excluding HVAC

Rooms

SIP: 0.0803 kg/liter

SIP: 0.0803 kg/liter
SIP: 0.0803 kg/liter
SIP: 0.0803 kg/liter
230 kg/load

36.135
36.14

36.14

64.24
0

64.24

36.135

230
266.14

230
230

36.135

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

10%

35.227

35.227

35.227

35.227

35.227

35.227

41.651

38.841

61.841

35.227

35.227

35.227

35.227

35.227

35.227

35.227

58.227

35.227

38.841

35.227

35.227

35.227

35.227

35.227

Total Pure Steam Usage

USP Water Supply, LPH
Time (Day. Hour)
Time-averaged production

kg/hr
782.285775

387.5
445.63
2.00

387.5
445.63
2.01

387.5
445.63
2.02

387.5
445.63
2.03

387.5
445.63
2.04

387.5
445.63
2.05

458.16
526.89
2.06

427.25
491.34
2.07

680.25
782.29
2.08

387.5
445.63
2.09

387.5
445.63
2.10

387.5
445.63
2.11

387.5
445.63
2.12

387.5
445.63
2.13

387.5
445.63
2.14

387.5
445.63
2.15

640.5
736.58
2.16

387.5
445.63
2.17

427.25
491.34
2.18

387.5
445.63
2.19

387.5
445.63
2.20

387.5
445.63
2.21

387.5
445.63
2.22

387.5
445.63
2.23

Maximum Pure Steam Load

Line loss, maximum
Blowdown
Safety factor
Pure steam generator
Pure Steam Pressure
Blowdown percentage
Minimum load

680
68.0
102.0
20%
1100
2.4
15%
35%

AHU (humidification)
Line Loss

407.5

Daily use less humidification

Daily use, total
Daily line loss

1541.4
9995.9
908.7

Histogram of Pure Steam Usage

Wednesday Time of Day (Hour of Day)

Sizing Assumptions

10

11

SIP UF115031

1500L Mammalian Purification Process Suite 1 Starts on Monday

12
SP-S LPC115021 WFI
Push

13

14

15

16

17

18

19

20

21

22

23

24

SIP UF115041

UF-115031 Viresolve (Operation)

Suite 1 Media Prep

Suite 1 Buffer Prep

SIP 800L BPT

B#9 Prep

HWFI rinse

B#10Prep

HWFI rinse

B#13Prep

800L BP TK
CIP

500 L Mammalian Cell Culture Process Suite 2 Starts on Monday

Use points
(max. consumption)
Building 1C
M1-CC-1 Mam Suite 1, Cell Culture
M1-P1 Mam Suite 1, V+ Purification
M1-P2 Mam Suite 1, V- Purification
Mam Suite 1 Buffer Preparation
FF-Auto Filling Suite Autoclave
End user load excluding HVAC

Rooms

SIP: 0.0803 kg/liter

SIP: 0.0803 kg/liter
SIP: 0.0803 kg/liter
SIP: 0.0803 kg/liter
230 kg/load

36.135
36.14
64.24
0

230
230

64.24

36.135

230
230

36.135

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

10%

35.227

35.227

35.227

35.227

35.227

58.227

41.651

38.841

35.227

35.227

35.227

58.227

35.227

35.227

35.227

35.227

38.841

35.227

35.227

35.227

35.227

35.227

35.227

35.227

Total Pure Steam Usage

USP Water Supply, LPH
Time (Day. Hour)
Time-averaged production

kg/hr
782.285775

387.5
445.63
3.00

387.5
445.63
3.01

387.5
445.63
3.02

387.5
445.63
3.03

387.5
445.63
3.04

640.5
736.58
3.05

458.16
526.89
3.06

427.25
491.34
3.07

387.5
445.63
3.08

387.5
445.63
3.09

387.5
445.63
3.10

640.5
736.58
3.11

387.5
445.63
3.12

387.5
445.63
3.13

387.5
445.63
3.14

387.5
445.63
3.15

427.25
491.34
3.16

387.5
445.63
3.17

387.5
445.63
3.18

387.5
445.63
3.19

387.5
445.63
3.20

387.5
445.63
3.21

387.5
445.63
3.22

387.5
445.63
3.23

Maximum Pure Steam Load

Line loss, maximum
Blowdown
Safety factor
Pure steam generator
Pure Steam Pressure
Blowdown percentage
Minimum load

680
68.0
102.0
20%
1100
2.4
15%
35%

AHU (humidification)
Line Loss

407.5

Daily use less humidification

Daily use, total
Daily line loss

1501.6
9956.2
905.1

Histogram of Pure Steam Usage

Thursday Time of Day (Hour of Day)

Sizing Assumptions

1500L Mammalian Purification Process Suite 1 Starts on Monday

10

11

12

13

14

15

16

17

18

19

IEC LPC115051 WFI

Push

UF-115041 (Operation)

20

21

22

23

24

SIP UF115061

Suite 1 Media Prep

Suite 1 Buffer Prep
500 L Mammalian Cell Culture Process Suite 2 Starts on Monday
Use points
(max. consumption)
Building 1C
M1-CC-1 Mam Suite 1, Cell Culture
M1-P1 Mam Suite 1, V+ Purification
M1-P2 Mam Suite 1, V- Purification
Mam Suite 1 Buffer Preparation
FF-Auto Filling Suite Autoclave
End user load excluding HVAC

Rooms

SIP: 0.0803 kg/liter

SIP: 0.0803 kg/liter
SIP: 0.0803 kg/liter
SIP: 0.0803 kg/liter
230 kg/load

36.14

230
230

230
230

36.135

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

10%

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

58.227

35.227

35.227

35.227

58.227

35.227

35.227

35.227

35.227

35.227

38.841

35.227

35.227

35.227

35.227

Total Pure Steam Usage

USP Water Supply, LPH
Time (Day. Hour)
Time-averaged production

kg/hr
782.285775

387.5
445.63
4.00

387.5
445.63
4.01

387.5
445.63
4.02

387.5
445.63
4.03

387.5
445.63
4.04

387.5
445.63
4.05

387.5
445.63
4.06

387.5
445.63
4.07

387.5
445.63
4.08

640.5
736.58
4.09

387.5
445.63
4.10

387.5
445.63
4.11

387.5
445.63
4.12

640.5
736.58
4.13

387.5
445.63
4.14

387.5
445.63
4.15

387.5
445.63
4.16

387.5
445.63
4.17

387.5
445.63
4.18

427.25
491.34
4.19

387.5
445.63
4.20

387.5
445.63
4.21

387.5
445.63
4.22

387.5
445.63
4.23

Maximum Pure Steam Load

Line loss, maximum
Blowdown
Safety factor
Pure steam generator
Pure Steam Pressure
Blowdown percentage
Minimum load

680
68.0
102.0
20%
1100
2.4
15%
35%

AHU (humidification)
Line Loss

407.5

Daily use less humidification

Daily use, total
Daily line loss

1391.2
9845.7
895.1

Histogram of Pure Steam Usage

Friday Time of Day (Hour of Day)

Sizing Assumptions

1500L Mammalian Purification Process Suite 1 Starts on Monday

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

SIP UF115061

UF-115061

Suite 1 Media Prep

Suite 1 Buffer Prep
500 L Mammalian Cell Culture Process Suite 2 Starts on Monday
Use points
(max. consumption)
Building 1C
M1-CC-1 Mam Suite 1, Cell Culture
M1-P1 Mam Suite 1, V+ Purification
M1-P2 Mam Suite 1, V- Purification
Mam Suite 1 Buffer Preparation
FF-Auto Filling Suite Autoclave
End user load excluding HVAC

Rooms

SIP: 0.0803 kg/liter

SIP: 0.0803 kg/liter
SIP: 0.0803 kg/liter
SIP: 0.0803 kg/liter
230 kg/load

36.135

230
230

230
266.14

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

10%

35.227

35.227

35.227

35.227

35.227

35.227

35.227

58.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

61.841

35.227

35.227

35.227

35.227

Total Pure Steam Usage

USP Water Supply, LPH
Time (Day. Hour)
Time-averaged production

kg/hr
782.285775

387.5
445.63
5.00

387.5
445.63
5.01

387.5
445.63
5.02

387.5
445.63
5.03

387.5
445.63
5.04

387.5
445.63
5.05

387.5
445.63
5.06

640.5
736.58
5.07

387.5
445.63
5.08

387.5
445.63
5.09

387.5
445.63
5.10

387.5
445.63
5.11

387.5
445.63
5.12

387.5
445.63
5.13

387.5
445.63
5.14

387.5
445.63
5.15

387.5
445.63
5.16

387.5
445.63
5.17

387.5
445.63
5.18

680.25
782.29
5.19

387.5
445.63
5.20

387.5
445.63
5.21

387.5
445.63
5.22

387.5
445.63
5.23

Maximum Pure Steam Load

Line loss, maximum
Blowdown
Safety factor
Pure steam generator
Pure Steam Pressure
Blowdown percentage
Minimum load

680
68.0
102.0
20%
1100
2.4
15%
35%

AHU (humidification)
Line Loss

407.5

Daily use less humidification

Daily use, total
Daily line loss

1391.2
9845.7
895.1

Histogram of Pure Steam Usage

Saturday Time of Day (Hour of Day)

Sizing Assumptions

8
SEC LPC115071 WFI
Push

1500L Mammalian Purification Process Suite 1 Starts on Monday

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

UF-115061 (Operation)

Suite 1 Media Prep

Suite 1 Buffer Prep
500 L Mammalian Cell Culture Process Suite 2 Starts on Monday
Use points
(max. consumption)
Building 1C
M1-CC-1 Mam Suite 1, Cell Culture
M1-P1 Mam Suite 1, V+ Purification
M1-P2 Mam Suite 1, V- Purification
Mam Suite 1 Buffer Preparation
FF-Auto Filling Suite Autoclave
End user load excluding HVAC

Rooms

SIP: 0.0803 kg/liter

SIP: 0.0803 kg/liter
SIP: 0.0803 kg/liter
SIP: 0.0803 kg/liter
230 kg/load
0

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

10%

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

Total Pure Steam Usage

USP Water Supply, LPH
Time (Day. Hour)
Time-averaged production

kg/hr
782.285775

387.5
445.63
6.00

387.5
445.63
6.01

387.5
445.63
6.02

387.5
445.63
6.03

387.5
445.63
6.04

387.5
445.63
6.05

387.5
445.63
6.06

387.5
445.63
6.07

387.5
445.63
6.08

387.5
445.63
6.09

387.5
445.63
6.10

387.5
445.63
6.11

387.5
445.63
6.12

387.5
445.63
6.13

387.5
445.63
6.14

387.5
445.63
6.15

387.5
445.63
6.16

387.5
445.63
6.17

387.5
445.63
6.18

387.5
445.63
6.19

387.5
445.63
6.20

387.5
445.63
6.21

387.5
445.63
6.22

387.5
445.63
6.23

Maximum Pure Steam Load

Line loss, maximum
Blowdown
Safety factor
Pure steam generator
Pure Steam Pressure
Blowdown percentage
Minimum load

680
68.0
102.0
20%
1100
2.4
15%
35%

AHU (humidification)
Line Loss

407.5

Daily use less humidification

Daily use, total
Daily line loss

845.45
9300
845.5

Histogram of Pure Steam Usage

Sunday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

352.27

10%

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

35.227

Total Pure Steam Usage

USP Water Supply, LPH
Time (Day. Hour)
Time-averaged production

kg/hr
782.285775

387.5
445.63
7.00

387.5
445.63
7.01

387.5
445.63
7.02

387.5
445.63
7.03

387.5
445.63
7.04

387.5
445.63
7.05

387.5
445.63
7.06

387.5
445.63
7.07

387.5
445.63
7.08

387.5
445.63
7.09

387.5
445.63
7.10

387.5
445.63
7.11

387.5
445.63
7.12

387.5
445.63
7.13

387.5
445.63
7.14

387.5
445.63
7.15

387.5
445.63
7.16

387.5
445.63
7.17

387.5
445.63
7.18

387.5
445.63
7.19

387.5
445.63
7.20

387.5
445.63
7.21

387.5
445.63
7.22

387.5
445.63
7.23

Maximum Pure Steam Load

Line loss, maximum
Blowdown
Safety factor
Pure steam generator
Pure Steam Pressure
Blowdown percentage
Minimum load

680
68.0
102.0
20%
1100
2.4
15%
35%

1500L Mammalian Purification Process Suite 1 Starts on Monday

Suite 1 Media Prep
Suite 1 Buffer Prep
500 L Mammalian Cell Culture Process Suite 2 Starts on Monday
Use points
(max. consumption)
Building 1C
M1-CC-1 Mam Suite 1, Cell Culture
M1-P1 Mam Suite 1, V+ Purification
M1-P2 Mam Suite 1, V- Purification
Mam Suite 1 Buffer Preparation
FF-Auto Filling Suite Autoclave
End user load excluding HVAC

Rooms

SIP: 0.0803 kg/liter

SIP: 0.0803 kg/liter
SIP: 0.0803 kg/liter
SIP: 0.0803 kg/liter
230 kg/load

AHU (humidification)
Line Loss

407.5

Daily use less humidification

Daily use, total
Daily line loss

845.45
9300
845.5

Histogram of Cell culture waste surge

Monday Time of Day (Hour of Day)

Sizing Assumptions

10

70

1153

1413

120

120

1410

11

12

13

14

15

16

17

18

19

21

22

23

24

Suite 1 biowaste

2957

Total biowaste

liter per hour

70

1153

1413

120

120

1410

1000

2957

46%

1.00
0
1800
2500
0.00%

1.01
0
1800
2500
0.00%

1.02
0
1800
2500
0.00%

1.03
0
1800
2500
0.00%

1.04
0
1800
2500
0.00%

1.05
0
1800
2500
0.00%

1.06
0
1800
2500
0.00%

1.07
0
1800
2500
0.00%

1.08
0
1800
2500
0.00%

1.09
0
1800
2500
0.00%

1.1
0
1800
2500
0.00%

1.11
0
1800
2500
0.00%

1.12
0
1800
2500
0.00%

1.13
0
1800
2500
0.00%

1.14
0
1800
2500
0.00%

1.15
0
1800
2500
0.00%

1.16
0
1800
2500
0.00%

1.17
0
1800
2500
0.00%

1.18
0
1800
2500
0.00%

1.19
1157
1800
2500
46.28%

1.2
0
1800
2500
0.00%

1.21
0
1800
2500
0.00%

1.22
0
1800
2500
0.00%

1.23
0
1800
2500
0.00%

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

Initial Tank Volume

Make-Up Rate
Tank Working Volume
Max inflow

User Selectable
2500
1800
2500
2957
49.28

Average Daily Usage

Maximum Daily Usage
L
Lph
L
Lph
liter/min

1177.57
8243

1000

20

2957

Daily Requirement,l

8243

Histogram of Cell culture waste surge

Tuesday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

Suite 1 biowaste

2957

Total biowaste

liter per hour

46%

2.00
0
1800
2500
0.00%

2.01
0
1800
2500
0.00%

2.02
0
1800
2500
0.00%

2.03
0
1800
2500
0.00%

2.04
0
1800
2500
0.00%

2.05
0
1800
2500
0.00%

2.06
0
1800
2500
0.00%

2.07
0
1800
2500
0.00%

2.08
0
1800
2500
0.00%

2.09
0
1800
2500
0.00%

2.10
0
1800
2500
0.00%

2.11
0
1800
2500
0.00%

2.12
0
1800
2500
0.00%

2.13
0
1800
2500
0.00%

2.14
0
1800
2500
0.00%

2.15
0
1800
2500
0.00%

2.16
0
1800
2500
0.00%

2.17
0
1800
2500
0.00%

2.18
0
1800
2500
0.00%

2.19
0
1800
2500
0.00%

2.20
0
1800
2500
0.00%

2.21
0
1800
2500
0.00%

2.22
0
1800
2500
0.00%

2.23
0
1800
2500
0.00%

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

Daily Requirement,l
Initial Tank Volume
Make-Up Rate
Tank Working Volume
Max inflow

User Selectable
2500
1800
2500
2957
49.28

Histogram of Cell culture waste surge

Wednesday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

Suite 1 biowaste

2957

Total biowaste

liter per hour

46%

3.00
0
1800
2500
0.00%

3.01
0
1800
2500
0.00%

3.02
0
1800
2500
0.00%

3.03
0
1800
2500
0.00%

3.04
0
1800
2500
0.00%

3.05
0
1800
2500
0.00%

3.06
0
1800
2500
0.00%

3.07
0
1800
2500
0.00%

3.08
0
1800
2500
0.00%

3.09
0
1800
2500
0.00%

3.10
0
1800
2500
0.00%

3.11
0
1800
2500
0.00%

3.12
0
1800
2500
0.00%

3.13
0
1800
2500
0.00%

3.14
0
1800
2500
0.00%

3.15
0
1800
2500
0.00%

3.16
0
1800
2500
0.00%

3.17
0
1800
2500
0.00%

3.18
0
1800
2500
0.00%

3.19
0
1800
2500
0.00%

3.20
0
1800
2500
0.00%

3.21
0
1800
2500
0.00%

3.22
0
1800
2500
0.00%

3.23
0
1800
2500
0.00%

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

Daily Requirement,l
Initial Tank Volume
Make-Up Rate
Tank Working Volume
Max inflow

User Selectable
2500
1800
2500
2957
49.28

Histogram of Cell culture waste surge

Thursday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

Suite 1 biowaste

2957

Total biowaste

liter per hour

46%

4.00
0
1800
2500
0.00%

4.01
0
1800
2500
0.00%

4.02
0
1800
2500
0.00%

4.03
0
1800
2500
0.00%

4.04
0
1800
2500
0.00%

4.05
0
1800
2500
0.00%

4.06
0
1800
2500
0.00%

4.07
0
1800
2500
0.00%

4.08
0
1800
2500
0.00%

4.09
0
1800
2500
0.00%

4.10
0
1800
2500
0.00%

4.11
0
1800
2500
0.00%

4.12
0
1800
2500
0.00%

4.13
0
1800
2500
0.00%

4.14
0
1800
2500
0.00%

4.15
0
1800
2500
0.00%

4.16
0
1800
2500
0.00%

4.17
0
1800
2500
0.00%

4.18
0
1800
2500
0.00%

4.19
0
1800
2500
0.00%

4.20
0
1800
2500
0.00%

4.21
0
1800
2500
0.00%

4.22
0
1800
2500
0.00%

4.23
0
1800
2500
0.00%

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

Daily Requirement,l
Initial Tank Volume
Make-Up Rate
Tank Working Volume
Max inflow

User Selectable
2500
1800
2500
2957
49.28

Histogram of Cell culture waste surge

Friday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

Suite 1 biowaste

2957

Total biowaste

liter per hour

46%

5.00
0
1800
2500
0.00%

5.01
0
1800
2500
0.00%

5.02
0
1800
2500
0.00%

5.03
0
1800
2500
0.00%

5.04
0
1800
2500
0.00%

5.05
0
1800
2500
0.00%

5.06
0
1800
2500
0.00%

5.07
0
1800
2500
0.00%

5.08
0
1800
2500
0.00%

5.09
0
1800
2500
0.00%

5.10
0
1800
2500
0.00%

5.11
0
1800
2500
0.00%

5.12
0
1800
2500
0.00%

5.13
0
1800
2500
0.00%

5.14
0
1800
2500
0.00%

5.15
0
1800
2500
0.00%

5.16
0
1800
2500
0.00%

5.17
0
1800
2500
0.00%

5.18
0
1800
2500
0.00%

5.19
0
1800
2500
0.00%

5.20
0
1800
2500
0.00%

5.21
0
1800
2500
0.00%

5.22
0
1800
2500
0.00%

5.23
0
1800
2500
0.00%

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

Daily Requirement,l
Initial Tank Volume
Make-Up Rate
Tank Working Volume
Max inflow

User Selectable
2500
1800
2500
2957
49.28

Histogram of Cell culture waste surge

Saturday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

Suite 1 biowaste

2957

Total biowaste

liter per hour

46%

6.00
0
1800
2500
0.00%

6.01
0
1800
2500
0.00%

6.02
0
1800
2500
0.00%

6.03
0
1800
2500
0.00%

6.04
0
1800
2500
0.00%

6.05
0
1800
2500
0.00%

6.06
0
1800
2500
0.00%

6.07
0
1800
2500
0.00%

6.08
0
1800
2500
0.00%

6.09
0
1800
2500
0.00%

6.10
0
1800
2500
0.00%

6.11
0
1800
2500
0.00%

6.12
0
1800
2500
0.00%

6.13
0
1800
2500
0.00%

6.14
0
1800
2500
0.00%

6.15
0
1800
2500
0.00%

6.16
0
1800
2500
0.00%

6.17
0
1800
2500
0.00%

6.18
0
1800
2500
0.00%

6.19
0
1800
2500
0.00%

6.20
0
1800
2500
0.00%

6.21
0
1800
2500
0.00%

6.22
0
1800
2500
0.00%

6.23
0
1800
2500
0.00%

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

Daily Requirement,l
Initial Tank Volume
Make-Up Rate
Tank Working Volume
Max inflow

User Selectable
2500
1800
2500
2957
49.28

Histogram of Cell culture waste surge

Sunday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

Suite 1 biowaste

2957

Total biowaste

liter per hour

46%

7.00
0
1800
2500
0.00%

7.01
0
1800
2500
0.00%

7.02
0
1800
2500
0.00%

7.03
0
1800
2500
0.00%

7.04
0
1800
2500
0.00%

7.05
0
1800
2500
0.00%

7.06
0
1800
2500
0.00%

7.07
0
1800
2500
0.00%

7.08
0
1800
2500
0.00%

7.09
0
1800
2500
0.00%

7.10
0
1800
2500
0.00%

7.11
0
1800
2500
0.00%

7.12
0
1800
2500
0.00%

7.13
0
1800
2500
0.00%

7.14
0
1800
2500
0.00%

7.15
0
1800
2500
0.00%

7.16
0
1800
2500
0.00%

7.17
0
1800
2500
0.00%

7.18
0
1800
2500
0.00%

7.19
0
1800
2500
0.00%

7.20
0
1800
2500
0.00%

7.21
0
1800
2500
0.00%

7.22
0
1800
2500
0.00%

7.23
0
1800
2500
0.00%

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

Daily Requirement,l
Initial Tank Volume
Make-Up Rate
Tank Working Volume
Max inflow

User Selectable
2500
1800
2500
2957
49.28

Throughput

Biowaste

2000

2000

1800

1800

1600

1600

1400

1400

1200

1200

1000

1000

800

800

600

600

400

400

200

200

Time (Day. Hour)

Throughput, liters/hr

Biowaste, liters

Cell Culture Biowaste Surge and Throughput

Histogram of Biowaste

Monday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

Suite 1 biowaste

2957

70

1153

1413

120

120

1410

1000

2957

Total biowaste

liter per hour

70

1153

1413

120

120

1410

1000

2957

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

58% maximum

1.00
0
1800
2000
0.00%

1.01
0
1800
2000
0.00%

1.02
0
1800
2000
0.00%

1.03
0
1800
2000
0.00%

1.04
0
1800
2000
0.00%

1.05
0
1800
2000
0.00%

1.06
0
1800
2000
0.00%

1.07
0
1800
2000
0.00%

1.08
0
1800
2000
0.00%

1.09
0
1800
2000
0.00%

1.1
0
1800
2000
0.00%

1.11
0
1800
2000
0.00%

1.12
0
1800
2000
0.00%

1.13
0
1800
2000
0.00%

1.14
0
1800
2000
0.00%

1.15
0
1800
2000
0.00%

1.16
0
1800
2000
0.00%

1.17
0
1800
2000
0.00%

1.18
0
1800
2000
0.00%

1.19
1157
1800
2000
57.85%

1.2
0
1800
2000
0.00%

1.21
0
1800
2000
0.00%

1.22
0
1800
2000
0.00%

1.23
0
1800
2000
0.00%

Initial Tank Inventory

Make-Up Rate
Tank Working Volume
Maximum Inflow
Time-averaged inflow

User Selectable
0
1800
2000
2957
49.1

Average Daily Usage

Maximum Daily Usage
L
Lph
L
Lph
Lph

30

Liters/min

49.2833 Liters/min
0.81776 Liters/min

1177.57
8243

Daily Requirement,l

8243

Histogram of Biowaste

Tuesday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

Suite 1 biowaste

2957

Total biowaste

liter per hour

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

58% maximum

2.00
0
1800
2000
0.00%

2.01
0
1800
2000
0.00%

2.02
0
1800
2000
0.00%

2.03
0
1800
2000
0.00%

2.04
0
1800
2000
0.00%

2.05
0
1800
2000
0.00%

2.06
0
1800
2000
0.00%

2.07
0
1800
2000
0.00%

2.08
0
1800
2000
0.00%

2.09
0
1800
2000
0.00%

2.10
0
1800
2000
0.00%

2.11
0
1800
2000
0.00%

2.12
0
1800
2000
0.00%

2.13
0
1800
2000
0.00%

2.14
0
1800
2000
0.00%

2.15
0
1800
2000
0.00%

2.16
0
1800
2000
0.00%

2.17
0
1800
2000
0.00%

2.18
0
1800
2000
0.00%

2.19
0
1800
2000
0.00%

2.20
0
1800
2000
0.00%

2.21
0
1800
2000
0.00%

2.22
0
1800
2000
0.00%

2.23
0
1800
2000
0.00%

Initial Tank Inventory

Make-Up Rate
Tank Working Volume
Maximum Inflow
Time-averaged inflow

User Selectable
0
1800
2000
2957
49.1

Daily Requirement,l

Histogram of Biowaste

Wednesday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

Suite 1 biowaste

2957

Total biowaste

liter per hour

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

58% maximum

3.00
0
1800
2000
0.00%

3.01
0
1800
2000
0.00%

3.02
0
1800
2000
0.00%

3.03
0
1800
2000
0.00%

3.04
0
1800
2000
0.00%

3.05
0
1800
2000
0.00%

3.06
0
1800
2000
0.00%

3.07
0
1800
2000
0.00%

3.08
0
1800
2000
0.00%

3.09
0
1800
2000
0.00%

3.10
0
1800
2000
0.00%

3.11
0
1800
2000
0.00%

3.12
0
1800
2000
0.00%

3.13
0
1800
2000
0.00%

3.14
0
1800
2000
0.00%

3.15
0
1800
2000
0.00%

3.16
0
1800
2000
0.00%

3.17
0
1800
2000
0.00%

3.18
0
1800
2000
0.00%

3.19
0
1800
2000
0.00%

3.20
0
1800
2000
0.00%

3.21
0
1800
2000
0.00%

3.22
0
1800
2000
0.00%

3.23
0
1800
2000
0.00%

Initial Tank Inventory

Make-Up Rate
Tank Working Volume
Maximum Inflow
Time-averaged inflow

User Selectable
0
1800
2000
2957
49.1

Daily Requirement,l

Histogram of Biowaste

Thursday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

Suite 1 biowaste

2957

Total biowaste

liter per hour

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

58% maximum

4.00
0
1800
2000
0.00%

4.01
0
1800
2000
0.00%

4.02
0
1800
2000
0.00%

4.03
0
1800
2000
0.00%

4.04
0
1800
2000
0.00%

4.05
0
1800
2000
0.00%

4.06
0
1800
2000
0.00%

4.07
0
1800
2000
0.00%

4.08
0
1800
2000
0.00%

4.09
0
1800
2000
0.00%

4.10
0
1800
2000
0.00%

4.11
0
1800
2000
0.00%

4.12
0
1800
2000
0.00%

4.13
0
1800
2000
0.00%

4.14
0
1800
2000
0.00%

4.15
0
1800
2000
0.00%

4.16
0
1800
2000
0.00%

4.17
0
1800
2000
0.00%

4.18
0
1800
2000
0.00%

4.19
0
1800
2000
0.00%

4.20
0
1800
2000
0.00%

4.21
0
1800
2000
0.00%

4.22
0
1800
2000
0.00%

4.23
0
1800
2000
0.00%

Initial Tank Inventory

Make-Up Rate
Tank Working Volume
Maximum Inflow
Time-averaged inflow

User Selectable
0
1800
2000
2957
49.1

Daily Requirement,l

Histogram of Biowaste

Friday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

Suite 1 biowaste

2957

Total biowaste

liter per hour

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

58% maximum

5.00
0
1800
2000
0.00%

5.01
0
1800
2000
0.00%

5.02
0
1800
2000
0.00%

5.03
0
1800
2000
0.00%

5.04
0
1800
2000
0.00%

5.05
0
1800
2000
0.00%

5.06
0
1800
2000
0.00%

5.07
0
1800
2000
0.00%

5.08
0
1800
2000
0.00%

5.09
0
1800
2000
0.00%

5.10
0
1800
2000
0.00%

5.11
0
1800
2000
0.00%

5.12
0
1800
2000
0.00%

5.13
0
1800
2000
0.00%

5.14
0
1800
2000
0.00%

5.15
0
1800
2000
0.00%

5.16
0
1800
2000
0.00%

5.17
0
1800
2000
0.00%

5.18
0
1800
2000
0.00%

5.19
0
1800
2000
0.00%

5.20
0
1800
2000
0.00%

5.21
0
1800
2000
0.00%

5.22
0
1800
2000
0.00%

5.23
0
1800
2000
0.00%

Initial Tank Inventory

Make-Up Rate
Tank Working Volume
Maximum Inflow
Time-averaged inflow

User Selectable
0
1800
2000
2957
49.1

Daily Requirement,l

Histogram of Biowaste

Saturday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

Suite 1 biowaste

2957

Total biowaste

liter per hour

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

58% maximum

6.00
0
1800
2000
0.00%

6.01
0
1800
2000
0.00%

6.02
0
1800
2000
0.00%

6.03
0
1800
2000
0.00%

6.04
0
1800
2000
0.00%

6.05
0
1800
2000
0.00%

6.06
0
1800
2000
0.00%

6.07
0
1800
2000
0.00%

6.08
0
1800
2000
0.00%

6.09
0
1800
2000
0.00%

6.10
0
1800
2000
0.00%

6.11
0
1800
2000
0.00%

6.12
0
1800
2000
0.00%

6.13
0
1800
2000
0.00%

6.14
0
1800
2000
0.00%

6.15
0
1800
2000
0.00%

6.16
0
1800
2000
0.00%

6.17
0
1800
2000
0.00%

6.18
0
1800
2000
0.00%

6.19
0
1800
2000
0.00%

6.20
0
1800
2000
0.00%

6.21
0
1800
2000
0.00%

6.22
0
1800
2000
0.00%

6.23
0
1800
2000
0.00%

Initial Tank Inventory

Make-Up Rate
Tank Working Volume
Maximum Inflow
Time-averaged inflow

User Selectable
0
1800
2000
2957
49.1

Daily Requirement,l

Histogram of Biowaste

Sunday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

Suite 1 biowaste

2957

Total biowaste

liter per hour

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

58% maximum

7.00
0
1800
2000
0.00%

7.01
0
1800
2000
0.00%

7.02
0
1800
2000
0.00%

7.03
0
1800
2000
0.00%

7.04
0
1800
2000
0.00%

7.05
0
1800
2000
0.00%

7.06
0
1800
2000
0.00%

7.07
0
1800
2000
0.00%

7.08
0
1800
2000
0.00%

7.09
0
1800
2000
0.00%

7.10
0
1800
2000
0.00%

7.11
0
1800
2000
0.00%

7.12
0
1800
2000
0.00%

7.13
0
1800
2000
0.00%

7.14
0
1800
2000
0.00%

7.15
0
1800
2000
0.00%

7.16
0
1800
2000
0.00%

7.17
0
1800
2000
0.00%

7.18
0
1800
2000
0.00%

7.19
0
1800
2000
0.00%

7.20
0
1800
2000
0.00%

7.21
0
1800
2000
0.00%

7.22
0
1800
2000
0.00%

7.23
0
1800
2000
0.00%

Initial Tank Inventory

Make-Up Rate
Tank Working Volume
Maximum Inflow
Time-averaged inflow

User Selectable
0
1800
2000
2957
49.1

Daily Requirement,l

Throughput

Biowaste

2500

2500

2250

2250

2000

2000

1750

1750

1500

1500

1250

1250

1000

1000

750

750

500

500

250

250

Time (Day. Hour)

Throughput, liters/hr

Inventory, liters

Biowaste (Kill System) Volume and Throughput

Histogram of Process Waste (includes killed biowaste)

Monday Time of Day (Hour of Day)

Sizing Assumptions

Suite 1 biowaste

2957

Fill/finish

1459

Clean steam condensate

10

11

12

13

70

1413

1413

120

120

150

959

14

15

1410

1206

500

1000

150

500

16

17

18

19

20

21

22

23

24

2957

959

856

266.135

70

64.24

230

120

120.45

230

liter per hour

140

1413

1413

334.24

120

2736

620.45

1150

500

230

959

856

2957

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

51% maximum

1.00
0
1800
2250
0.00%

1.01
0
1800
2250
0.00%

1.02
0
1800
2250
0.00%

1.03
0
1800
2250
0.00%

1.04
0
1800
2250
0.00%

1.05
0
1800
2250
0.00%

1.06
0
1800
2250
0.00%

1.07
0
1800
2250
0.00%

1.08
0
1800
2250
0.00%

1.09
936
1800
2250
41.60%

1.1
0
1800
2250
0.00%

1.11
0
1800
2250
0.00%

1.12
0
1800
2250
0.00%

1.13
0
1800
2250
0.00%

1.14
0
1800
2250
0.00%

1.15
0
1800
2250
0.00%

1.16
0
1800
2250
0.00%

1.17
0
1800
2250
0.00%

1.18
0
1800
2250
0.00%

1.19
1157
1800
2250
51.42%

1.2
0
1800
2250
0.00%

1.21
0
1800
2250
0.00%

1.22
0
1800
2250
0.00%

1.23
0
1800
2250
0.00%

Initial Tank Inventory

Throughput Rate
Tank Working Volume
Maximum Inflow
Time-averaged inflow

User Selectable
0
1800
2250
2957
210.7

Total process waste

Average Daily Usage

Maximum Daily Usage
L
Lph
L
Lph
Lph

30

Liters/min

49.283 Liters/min
3.5121 Liters/min

5057.4
13429
35402

Daily Requirement,l

13429

Histogram of Process Waste (includes killed biowaste)

Tuesday Time of Day (Hour of Day)

Sizing Assumptions

Suite 1 biowaste

2957

Fill/finish

1459

Clean steam condensate

10

11

12

13

150

1459

1206

14

15

16

650

17

18

19

20

21

22

23

24

959

856

266.135

64.24

36.135

266.14

230

36.135

liter per hour

64.24

36.135

416.14

1459

1206

650

1189

856

36.135

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

51% maximum

2.00
0
1800
2250
0.00%

2.01
0
1800
2250
0.00%

2.02
0
1800
2250
0.00%

2.03
0
1800
2250
0.00%

2.04
0
1800
2250
0.00%

2.05
0
1800
2250
0.00%

2.06
0
1800
2250
0.00%

2.07
0
1800
2250
0.00%

2.08
0
1800
2250
0.00%

2.09
0
1800
2250
0.00%

2.10
0
1800
2250
0.00%

2.11
0
1800
2250
0.00%

2.12
0
1800
2250
0.00%

2.13
0
1800
2250
0.00%

2.14
0
1800
2250
0.00%

2.15
0
1800
2250
0.00%

2.16
0
1800
2250
0.00%

2.17
0
1800
2250
0.00%

2.18
0
1800
2250
0.00%

2.19
0
1800
2250
0.00%

2.20
0
1800
2250
0.00%

2.21
0
1800
2250
0.00%

2.22
0
1800
2250
0.00%

2.23
0
1800
2250
0.00%

Initial Tank Inventory

Throughput Rate
Tank Working Volume
Maximum Inflow
Time-averaged inflow

User Selectable
0
1800
2250
2957
210.7

Total process waste

Daily Requirement,l

5912.6

Histogram of Process Waste (includes killed biowaste)

Wednesday Time of Day (Hour of Day)

Sizing Assumptions

Suite 1 biowaste

2957

Fill/finish

1459

Clean steam condensate

10

11

12

150

959

1206

500

13

14

15

16

17

18

19

20

21

22

23

24

150

500

959

706

150

266.135

230

64.24

36.135

230

36.135

liter per hour

230

64.24

186.14

959

1206

500

230

150

500

959

706

186.14

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

51% maximum

3.00
0
1800
2250
0.00%

3.01
0
1800
2250
0.00%

3.02
0
1800
2250
0.00%

3.03
0
1800
2250
0.00%

3.04
0
1800
2250
0.00%

3.05
0
1800
2250
0.00%

3.06
0
1800
2250
0.00%

3.07
0
1800
2250
0.00%

3.08
0
1800
2250
0.00%

3.09
0
1800
2250
0.00%

3.10
0
1800
2250
0.00%

3.11
0
1800
2250
0.00%

3.12
0
1800
2250
0.00%

3.13
0
1800
2250
0.00%

3.14
0
1800
2250
0.00%

3.15
0
1800
2250
0.00%

3.16
0
1800
2250
0.00%

3.17
0
1800
2250
0.00%

3.18
0
1800
2250
0.00%

3.19
0
1800
2250
0.00%

3.20
0
1800
2250
0.00%

3.21
0
1800
2250
0.00%

3.22
0
1800
2250
0.00%

3.23
0
1800
2250
0.00%

Initial Tank Inventory

Throughput Rate
Tank Working Volume
Maximum Inflow
Time-averaged inflow

User Selectable
0
1800
2250
2957
210.7

Total process waste

Daily Requirement,l

5876.5

Histogram of Process Waste (includes killed biowaste)

Thursday Time of Day (Hour of Day)

Sizing Assumptions

Suite 1 biowaste

2957

Fill/finish

1459

Clean steam condensate

10

11

12

13

150

500

500

14

15

650

16

17

18

959

706

19

20

21

22

23

24

706

266.135

230

230

36.135

liter per hour

150

730

500

880

959

706

706

36.135

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

51% maximum

4.00
0
1800
2250
0.00%

4.01
0
1800
2250
0.00%

4.02
0
1800
2250
0.00%

4.03
0
1800
2250
0.00%

4.04
0
1800
2250
0.00%

4.05
0
1800
2250
0.00%

4.06
0
1800
2250
0.00%

4.07
0
1800
2250
0.00%

4.08
0
1800
2250
0.00%

4.09
0
1800
2250
0.00%

4.10
0
1800
2250
0.00%

4.11
0
1800
2250
0.00%

4.12
0
1800
2250
0.00%

4.13
0
1800
2250
0.00%

4.14
0
1800
2250
0.00%

4.15
0
1800
2250
0.00%

4.16
0
1800
2250
0.00%

4.17
0
1800
2250
0.00%

4.18
0
1800
2250
0.00%

4.19
0
1800
2250
0.00%

4.20
0
1800
2250
0.00%

4.21
0
1800
2250
0.00%

4.22
0
1800
2250
0.00%

4.23
0
1800
2250
0.00%

Initial Tank Inventory

Throughput Rate
Tank Working Volume
Maximum Inflow
Time-averaged inflow

User Selectable
0
1800
2250
2957
210.7

Total process waste

Daily Requirement,l

4667.1

Histogram of Process Waste (includes killed biowaste)

Friday Time of Day (Hour of Day)

Sizing Assumptions

Suite 1 biowaste

2957

Fill/finish

1459

Clean steam condensate

10

11

12

150

959

1206

500

13

14

15

16

17

18

19

20

21

22

23

24

150

500

200

500

856

266.135

230

266.14

liter per hour

380

959

1206

500

150

500

200

500

856

266.14

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

51% maximum

5.00
0
1800
2250
0.00%

5.01
0
1800
2250
0.00%

5.02
0
1800
2250
0.00%

5.03
0
1800
2250
0.00%

5.04
0
1800
2250
0.00%

5.05
0
1800
2250
0.00%

5.06
0
1800
2250
0.00%

5.07
0
1800
2250
0.00%

5.08
0
1800
2250
0.00%

5.09
0
1800
2250
0.00%

5.10
0
1800
2250
0.00%

5.11
0
1800
2250
0.00%

5.12
0
1800
2250
0.00%

5.13
0
1800
2250
0.00%

5.14
0
1800
2250
0.00%

5.15
0
1800
2250
0.00%

5.16
0
1800
2250
0.00%

5.17
0
1800
2250
0.00%

5.18
0
1800
2250
0.00%

5.19
0
1800
2250
0.00%

5.20
0
1800
2250
0.00%

5.21
0
1800
2250
0.00%

5.22
0
1800
2250
0.00%

5.23
0
1800
2250
0.00%

Initial Tank Inventory

Throughput Rate
Tank Working Volume
Maximum Inflow
Time-averaged inflow

User Selectable
0
1800
2250
2957
210.7

Total process waste

Daily Requirement,l

5517.1

Histogram of Process Waste (includes killed biowaste)

Saturday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

266.135

liter per hour

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

51% maximum

6.00
0
1800
2250
0.00%

6.01
0
1800
2250
0.00%

6.02
0
1800
2250
0.00%

6.03
0
1800
2250
0.00%

6.04
0
1800
2250
0.00%

6.05
0
1800
2250
0.00%

6.06
0
1800
2250
0.00%

6.07
0
1800
2250
0.00%

6.08
0
1800
2250
0.00%

6.09
0
1800
2250
0.00%

6.10
0
1800
2250
0.00%

6.11
0
1800
2250
0.00%

6.12
0
1800
2250
0.00%

6.13
0
1800
2250
0.00%

6.14
0
1800
2250
0.00%

6.15
0
1800
2250
0.00%

6.16
0
1800
2250
0.00%

6.17
0
1800
2250
0.00%

6.18
0
1800
2250
0.00%

6.19
0
1800
2250
0.00%

6.20
0
1800
2250
0.00%

6.21
0
1800
2250
0.00%

6.22
0
1800
2250
0.00%

6.23
0
1800
2250
0.00%

Initial Tank Inventory

Throughput Rate
Tank Working Volume
Maximum Inflow
Time-averaged inflow

User Selectable
0
1800
2250
2957
210.7

Suite 1 biowaste

2957

Fill/finish

1459

Clean steam condensate

Total process waste

Daily Requirement,l

Histogram of Process Waste (includes killed biowaste)

Sunday Time of Day (Hour of Day)

Sizing Assumptions

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

266.135

liter per hour

Time (Day. Hour)

Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume

51% maximum

7.00
0
1800
2250
0.00%

7.01
0
1800
2250
0.00%

7.02
0
1800
2250
0.00%

7.03
0
1800
2250
0.00%

7.04
0
1800
2250
0.00%

7.05
0
1800
2250
0.00%

7.06
0
1800
2250
0.00%

7.07
0
1800
2250
0.00%

7.08
0
1800
2250
0.00%

7.09
0
1800
2250
0.00%

7.10
0
1800
2250
0.00%

7.11
0
1800
2250
0.00%

7.12
0
1800
2250
0.00%

7.13
0
1800
2250
0.00%

7.14
0
1800
2250
0.00%

7.15
0
1800
2250
0.00%

7.16
0
1800
2250
0.00%

7.17
0
1800
2250
0.00%

7.18
0
1800
2250
0.00%

7.19
0
1800
2250
0.00%

7.20
0
1800
2250
0.00%

7.21
0
1800
2250
0.00%

7.22
0
1800
2250
0.00%

7.23
0
1800
2250
0.00%

Initial Tank Inventory

Throughput Rate
Tank Working Volume
Maximum Inflow
Time-averaged inflow

User Selectable
0
1800
2250
2957
210.7

Suite 1 biowaste

2957

Fill/finish

1459

Clean steam condensate

Total process waste

Daily Requirement,l

Throughput

Process waste

2500

2500

2250

2250

2000

2000

1750

1750

1500

1500

1250

1250

1000

1000

750

750

500

500

250

250

Time (Day. Hour)

Throughput, liters/hr

Inventory, liters

Process Waste Volume and Throughput

NOTE: CIP volumes are based on an assumption of a flowrate of 3.46 liters/minute per meter of vessel circumference. A total
cycle time of 22 minutes is assumed, broken down as follows:
Initial rinse
Caustic wash
Second rinse
Acid wash
Final rinse

2 minutes USP PW
2 minutes USP PW
4 minutes USP PW
2 minutes USP PW
12 minutes WFI

2:1 fermentors/bioreactors
Vessel CIP Fluid,
Volume, L
L
25
647
30
688
60
867
70
912
75
934
100
1028
150
1176
200
1294
250
1394
300
1482
350
1560
400
1632
450
1697
500
1758
600
1868
700
1966
750
2012
800
2056
1600
2590

USP PW
WPU
Correl'n
294
294
313
313
394
394
415
415
425
424
467
467
535
535
588
589
634
634
674
674
709
709
742
742
771
771
799
799
849
849
894
894
915
915
935
935
1177
1177

WFI Final Rinse, L

WFI
Correl'n
353
353
375
375
473
473
497
497
509
509
561
560
641
641
706
706
760
761
808
808
851
851
890
890
926
925
959
958
1019
1019
1072
1072
1097
1097
1121
1121
1413
1413

USP PW Rinse
WFI Final Rinse
Tank volume/water volumeTank volume/water volume
correlation
correlation
Power Fit: y=ax^b
Power Fit: y=ax^b
Coefficient Data:
Coefficient Data:
a=
100.6452
a=
120.6076
b=
0.333367
b=
0.333541

Total CIP Flow

(for waste sizing)
Gal/min
LPH
8
29.41
1765
8
31.27
1876
10
39.40
2364
11
41.48
2489
11
42.44
2547
12
46.71
2803
14
53.49
3209
16
58.86
3531
17
63.40
3804
18
67.37
4042
19
70.92
4255
20
74.15
4449
20
77.11
4627
21
79.88
4793
22
84.88
5093
24
89.36
5362
24
91.44
5486
25
93.43
5606
31
117.71
7063

1:1 tanks/vessels
CIP Fluid,
L
815
867
1092
1149
1177
1295
1482
1630
1756
1867
1965
2056
2138
2215
2354
2477
2535
2590
3263

USP PW *
WPU
Correl'n
371
371
394
394
497
496
522
523
535
535
589
589
673
674
741
742
798
799
849
849
893
894
935
934
972
972
1007
1006
1070
1070
1126
1126
1152
1152
1177
1177
1483
1483

USP PW Rinse
Tank volume/water volume
correlation
Power Fit: y=ax^b
Coefficient Data:
a=
126.7826
b=
0.333362

WFI Final Rinse, L

WFI
Correl'n
445
445
473
473
596
596
627
627
642
642
706
706
808
808
889
890
958
959
1018
1019
1072
1072
1122
1121
1166
1166
1208
1208
1284
1284
1351
1351
1383
1383
1413
1413
1780
1780

WFI Final Rinse

Tank volume/water volume
correlation
Power Fit: y=ax^b
Coefficient Data:
a=
152.035
b = 0.333481

Total CIP Flow

(for waste sizing)
Gal/min
LPM
LPH
9.79
37.06
2223.46
10.41
39.39
2363.67
13.11
49.64
2978.43
13.81
52.26
3135.48
14.13
53.47
3208.42
15.55
58.86
3531.32
17.80
67.39
4043.43
19.59
74.15
4449.19
21.10
79.88
4792.74
22.42
84.88
5093.05
23.61
89.36
5361.59
24.68
93.43
5605.62
25.66
97.15
5829.06
26.59
100.64
6038.48
28.25
106.95
6416.84
29.74
112.59
6755.18
30.43
115.21
6912.33
31.10
117.71
7062.64
39.18
148.31
8898.37