Neurodegeneration Models & Services

As the human population ages, investigators are placing greater emphasis on neurodegenerative disorder research, and Taconic is responding with animal models uniquely suited to these studies. Taconic provides fast, easy access to valuable models for the study of Alzheimers disease, Parkinsons disease, ALS and other disorders including many models available exclusively from Taconic and offered with full use licenses.

Taconic Transgenic ModelsTM & Emerging Models AD11 mouse APPSWE mouse Tau mouse APPSWE-Tau mouse APOE2 mouse APOE3 mouse APOE4 mouse SOD1 rat

Traditional Animal Models Black 6 (male mice up to 12 months of age)

Molecular Analysis Services Pde6brd1 Retinal Degeneration Genotyping Assay Nnt Mutation Testing

Surgically Modified Models

Contract Research & Breeding Services

Samaritan Alzheimers Rat Model Unilateral 6 Hydroxydopamine Lesion of the Nigrostriatal Pathway Stroke Models - Cerebral Ischemia and Reperfusion and Endothelin-1 Induction of Cerebral Ischemia Microdialysis Implants Brain Cannulations

Disease Assessment Protocols Tissue Collection Parkinsons Disease Induction Protocol for Mice Aging of Any Commercial or Proprietary Line Phenotyping Services through PhenoPro

This booklet presents abridged descriptions. For additional information on model origins and pricing as well as model datasheets and references, please see the Taconic website at www.taconic.com.

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Taconic Transgenic Exchange | Taconic Transgenic Models

Taconic Transgenic Models (TTMs) are important research tools offered off the shelf in typical study quantities. Each TTM carries a label license granting you a license under Taconics in-licensed patent right(s) to use the model in your research. Taconic is the only commercial breeder that can supply genetically modified models with full licensing for use in your research.

AD11 Microinjected Mouse

B6;SJL Mixed Background Nomenclature: B6;SJL-Tg(CMV-Igk@aD11)BCat Tg(CMV-Igh@aD11)CCat

Alzheimers Disease Model

Expresses recombinant anti-nerve growth factor (NGF) antibodies (mAb aD11) Develops amyloid plaques, tau hyperphosphorylation, neurofibrillary tangles, neuronal loss and cognitive deficits Red and pink eyed animals, associated with certain coat colors, and the Pde6brd1 retinal degeneration mutation can cause light sensitivity and/or blindness in some animals. Upon request, mice can be screened for eye color and/or rd1 homozygosity for an additional fee.
Model Number Zygosity

008378-TT

Hemizygous/Hemizygous

APPSWE Microinjected Mouse

B6;SJL Mixed Background Nomenclature: B6;SJL-Tg(APPSWE)2576Kha

Alzheimers Disease Model

Carries a transgene coding for the 695-amino acid isoform of human Alzheimer -amyloid precursor protein carrying the Swedish mutation Expresses high concentrations of the mutant A, develops significant amyloid plaques and displays memory deficits Red and pink eyed animals, associated with certain coat colors, and the Pde6brd1 retinal degeneration mutation can cause light sensitivity and/or blindness in some animals. Upon request, mice can be screened for eye color and/or rd1 homozygosity for an additional fee.
Model Number Zygosity Model Number Zygosity

001349-T 001349-T-RD1

Hemizygous Hemizygous for AAPSWE: Wild Type or Heterozygous for rd1

001349-W Wild Type 001349-W-RD1 Wild Type for APPSWE; Wild Type or Heterozygous for rd1

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Taconic Transgenic Exchange | Taconic Transgenic Models

APPSWE Microinjected Mouse

129S6 Background Nomenclature: 129S6.Cg-Tg(APPSWE)2576Kha N20+?

Alzheimers Disease Model

Carries a transgene coding for the 695-amino acid isoform of human Alzheimer -amyloid precursor protein carrying the Swedish mutation Expresses high concentrations of the mutant A, develops significant amyloid plaques and displays memory deficits This background does not carry the Pde6brd1 retinal degeneration mutation and all animals are white-bellied agouti with pigmented eyes. The 129S6 strain (and all 129 substrains) carries a Disc1 mutation.
Model Number Zygosity Model Number Zygosity

002789-T

Hemizygous

002789-W

Wild Type

Tau Microinjected Mouse

Mixed C57BL/6, DBA/2, SW Background Nomenclature: STOCK-Tg(Prnp-MAPT*P301L)JNPL3Hlmc

Alzheimers Disease Model

Carries the transgene for the human P301L mutation of the microtubule-associated protein tau gene (MAPT) Develops behavioral and motor disturbances related to development of neuro-fibrillary tangles (NFT)
Model Number Zygosity Model Number Zygosity

002508-M 001638-T

Homozygous Hemizygous

001638-W

Wild Type

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Taconic Transgenic Exchange | Taconic Transgenic Models

APPSWE-Tau Microinjected Mouse

Alzheimers Disease Model

Mixed C57BL/6, DBA/2, SJL, SW Background Nomenclature: STOCK-Tg(APPSWE)2576Kha Tg(Prnp-MAPT*P301L)JNPL3Hlmc

Carries a transgene coding for the 695-amino acid isoform of human Alzheimer -amyloid precursor protein carrying the Swedish mutation in addition to the transgene for the human P301L mutation of the microtubule-associated protein tau gene (MAPT) Model 002469-TT are hemizygous for the APPSWE transgene and hemizygous for the Tau transgene Model 002469-WT mice are wild type for the APPSWE transgene and hemizygous for the Tau transgene and serve as one control for the 002469-TT model Model 003273-WW contains the same strain and stock genetic contribution as 002469, but in different proportions, and serves as the double wild type control Pink eyed animals, associated with certain coat color, and the Pde6brd1 retinal degeneration mutation, can cause light sensitivity and/or blindness in some animals. This may impact the results of behavioral testing. Upon request, animals can be screened for eye color and/or rd1 homozygosity for an additional fee.
Model Number Zygosity Model Number Zygosity

002469-TT Hemizygous/Hemizygous 002469-TT-RD1 Hemizygous/Hemizygous; Wild Type or Heterozygous for rd1

Nomenclature: STOCK
Model Number Zygosity

002469-WT Wild Type/Hemizygous 002469-WT-RD1 Wild Type/Hemizygous; Wild Type or Heterozygous for rd1

003273-WW Wild Type/Wild Type 003273-WW-RD1 Wild Type/Wild Type; Wild Type or Heterozygous for rd1

APOE2 Humanized Knock in

C57BL/6 Background Nomenclature: B6.129P2-Apoetm1(APOE*4)Mae N9

Alzheimers Disease Research

Homozygous for a human APOE*2 gene targeted replacement of the mouse Apoe gene Displays spontaneous atherogenesis, which is exacerbated by a high fat diet The APOE*2 allele appears to decrease the risk and delays onset of Alzheimers disease in humans
Model Number Zygosity

001547-M

Homozygous

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Taconic Transgenic Exchange | Taconic Transgenic Models

APOE3 Humanized Knock in

C57BL/6 Background Nomenclature: B6.129P2-Apoetm3(APOE*3)Mae N8

Alzheimers Disease Research

Homozygous for a human APOE*3 gene targeted replacement of the mouse Apoe gene Does not display spontaneous atherogenesis on a normal diet, but is susceptible on a high fat diet APOE*3 is the most common human APOE isoform
Model Number Zygosity

001548-M

Homozygous

APOE4 Humanized Knock in

C57BL/6 Background Nomenclature: B6.129P2-Apoetm3(APOE*4)Mae N8

Alzheimers Disease Research

Homozygous for a human APOE*4 gene targeted replacement of the endogenous mouse Apoe gene Exhibits an increased risk of atherosclerosis compared with wild type and APOE3 targeted replacement mice The APOE*4 allele has been implicated as a risk factor for developing Alzheimers disease in humans
Model Number Zygosity

001549-M

Homozygous

Taconic Transgenic Exchange | Emerging Models

Emerging Models are investigator-sponsored models produced and distributed by Taconic. As the sponsor sets distribution requirements for each Emerging Models, execution of an MTA may be required before ordering individual Emerging Models.

SOD1 Microinjected Rat

Sprague Dawley Background Nomenclature: NTac:SD-Tg(SOD1G93A)L26H

ALS Disease Model

Carries the transgene encoding the human SOD1 gene with the G93A mutation Hemizygous rats develop motor neuron disease, with abnormal gait and hind limb paralysis evident Disease onset can vary from individual to individual Developed as a joint collaboration between Wyeth and The ALS Association
Model Number Zygosity Model Number Zygosity

002148-T

Hemizygous

002148-W

Wild Type

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Traditional Models

Black 6 INBRED MOUSE

Nomenclature: C57BL/6NTac

Aging Model

The inbred strain most widely used as the genetic background for transgenic and mutant mice Taconics B6 sublines do not carry the known Nnt mutation Taconic now offers a ready inventory of B6 male mice up to 12 months in age B6 male mice are reduced density virgin cagemate B6 males, not retired breeders Save time and facility space for your aging research Any of Taconics commercial lines or your proprietary line raised here at Taconic can be aged to your specifications
Model Number

B6-M

Molecular Analysis Services

Pde6b Retinal Degeneration Genotyping Assay

rd1

Animals homozygous for the Pde6brd1 allele become blind by weaning. The presence of this allele may be very important in mice used for behavioral testing. This assay may be used to screen out Pde6brd1 homozygotes prior to behavioral work.
Model Number

GENO_RD1_PCR

Nnt Mutation Testing

Nnt (nicotinamide nucleotide transhydrogenase), a gene present in humans, mice and bacteria, is important to cellular metabolism and under conditions of stress can help in the detoxification of the cell. When a mutation of the Nnt gene occurs, a truncated Nnt protein can affect the phenotype, often resulting in abnormal glucose metabolism under conditions of stress. This property makes Nnt important in research that affects biological systems such as neurodegenerative diseases, diabetes, cancer, aging and cardiomyopathy. To ensure that your transgenics are free of the Nnt mutation, use Taconics Nnt mutation screening service. To learn more about Nnt mutation and order our screening service please visit www.taconic.com/nnt
Model Number

GENO-NNT-TEST

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Surgically Modified Models

Samaritan Alzheimers Rat Model

Alzheimers Disease Model

Induced by slow release of a proprietary mix of chemicals into the lateral ventricle of brain Over 4 weeks, animals develop memory impairment accompanied by increase in hyperphorylated Tau protein levels in the CSF Displays thioglavin-S-positive amyloid deposits, neuronal loss and gliosis

Unilateral 6-Hydroxydopamine Lesion of the Nigrostriatal Pathway

Parkinsons Disease Model

The unilateral destruction of dopamine neurons causes a chemical imbalance of the brain hemispheric content of the neurotransmitter dopamine This asymmetric stimulation is behaviorally manifested by locomotion in the direction of the unaffected hemisphere; the animal runs in circles. The quantification of circling behavior can be used to assess the efficacy of therapeutic agents which may be used in the treatment of Parkinsons disease. Following the 21 day evaluation, only animals with positive results (total of 180 rotations per 30 minutes or multiple 5 minute periods of >= 6 rotations/minute) are shipped. All rotational data is included with the animals.

Microdialysis Implants
A microdialysis guide cannula is implanted at specific target areas of the brain. Target areas include: hippocampus, prefrontal and striatal portions of the brain as well as custom coordinates available upon request. General applications include: drug metabolism and pharmacokinetics, behavioral studies, psychological research, neurological and neurochemical studies, addiction and chemical dependency

Brain Cannulations
Intracerebroventricular cannulations of the lateral ventricles Third ventricle cannulations Custom coordinate brain cannulations

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Contract Research Solutions

Phenotyping Services with PhenoPro

Phenotyping of Taconic neurodegeneration models, or any other rodent neurodegenerative model, can be completed with customized studies that involve testing for behavior, cognition, pain and sensory-motricity.

Alzheimers Disease Assessment Protocols for Rats and Mice

Useful for testing effects of therapeutic agents in preventing memory loss associated with Alzheimers disease and other neurodegenerative disorders. Any model can be aged for a study and end points include perfusion and fixation of central nervous system tissue for histological analyses, along with study reports.

Parkinsons Disease Induction Protocol for Mice

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is systemically administered to adult C57BL/6NTac mice. MPTP protocol can be combined with pharmacokinetic studies. Study endpoints can include receipt of the mice or fixed or frozen brains.

Parkinsons Disease Assessment Protocols or Rats and Mice

Useful for testing effects of therapeutic agents in attenuating declines in motor behavior associated with Parkinsons disease an other neurodegenerative disorders. Any model can be aged for a study and end points include perfusion and fixation of central and/or peripheral nervous system tissues for histological analyses, along with study reports.

Contract Breeding Solutions

Aging Protocol
Taconic offers a wide range of genetic or induced rodent models of Alzheimers and Parkinsons diseases, and Taconic offers customized aging studies that include the resources to allow for natural aging. Any commercial line can be held for aging under Isolated Barrier Conditions at competitive prices.
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