Ultrahigh-performance quantitation and identification for drug development

API

4000
LC/MS/MS System

perfor
A new level in LC/MS/MS
Performance in a class by itself
Performance at the highest level requires a company with solid experience and a sound knowledge of the drug development challenges that scientists face. Applied Biosystems/MDS SCIEX has served the pharmaceutical industry since 1989 with state-of-the-art technology and increasingly more powerful triple quadrupole mass spectrometers.

Highest sensitivity for drug development

The API 4000 LC/MS/MS System brings unparalleled performance for quantitation and identification to every phase of the pharmaceutical development process, from discovery to clinical trials. The system addresses the most pressing drug development challenges, whether you are a novice or an expert user. Drug development scientists have consistently relied on Applied Biosystems/ MDS SCIEX for top-performing mass spectrometry systems. Our atmospheric pressure ionization (API) LC/MS/MS systems have set the standard for sensitivity, robustness, and dependability. The API 4000 LC/MS/MS System sets a new standard of performance for the pharmaceutical industry. It delivers the most sensitive system for drug development and features improved robustness and throughput.

Maximum ionization efficiency Unmatched sensitivity

mance
Highest signal-to-noise ratio
The API 4000 systemthe next step in LC/MS/MS Plug-and-play probes
Easy to usenew Turbo V source permits rapid change between APCI (atmospheric pressure chemical ionization) and TurboIonSpray probes for effortless flexibility.

Optimized ion path

Higher throughputfrom the advanced ion source to the reliable interface to the patented LINAC collision cell, the ion path is designed to accelerate productivity.

New ceramic interface

Unparalleled ruggednessproven Curtain Gas interface technology combined with improved gas dynamics in the housing reduces maintenance and increases uptime.

Innovative Turbo V source

Best detection limit available improved ionization efficiency, reduced chemical noise, and enhanced gas dynamics enable lowest levels of quantitation.

Superior quantitation and identification

The API 4000 LC/MS/MS System brings unparalleled performance for quantitation and identification of lowabundance compounds in complex matrices. Through every phase of the pharmaceutical development process, from discovery to clinical trials, the API 4000 system can help you deliver new products to market sooner.

Greater sensitivity and ease of use make all the difference

Advanced Turbo V source improves lab results
The new Turbo V source for the API 4000 system maximizes ionization efficiency by combining embedded ceramic heater technology with improved gas dynamics. The increased ion yield in the gas phase translates into greater sensitivity over the wide range of liquid flow rates used in drug metabolism and pharmacokinetic (DMPK) analyses. The dual heater in the Turbo V source provides greater efficiency in ionization. Improved heat transfer and gas dynamics provide a tenfold increase in sensitivity. Higher sensitivity
Exhaust
Intensity

sensit
New source design features highly efficient ionization probes
Exclusive Turbo V source design for the API 4000 system
Heater Heater Heater gas Orifice Spray Heater gas
1.0 Time, min 1.5 2.0

50 fg 1 mL/min S/N 30

0 0.5

With the new Turbo V source, you can switch between APCI and TurboIonSpray probes in seconds. Determining the optimal ionization technique during method development is now simpler and faster.

The TurboIonSpray probe significantly increases sensitivity at high flow rates without stream splitting.

Higher throughput Faster method development

tivity
Analysis of a wider range of compounds Rugged, reliable operation
In the API 4000 system, the new APCI probe significantly reduces peak tailing from cross-contamination and other sources of chemical noise. Absorptive residues are completely eliminated because samples are instantly vaporized at higher temperatures. The probes rugged reliability ensures uninterrupted operation and repeatabilityday in and day out. Higher throughput The APCI probe provides: Wider flow range (502,000 L/minute) for greater flexibility in high-throughput applications Less thermal degradation of samples, improving sensitivity for a wider range of samples Reduced ionization suppression to enhance sensitivity and throughput Self-cleaning ceramic material to significantly reduce downtime for maintenance
Intensity

High-temperature APCI (500 pg injections)

0 0 1 2 3 4 Time, min 5 6 7 8

The new Turbo V source virtually eliminates cross-contamination, even with large sample loads. Injection of 5-fluorouracil every 30 seconds demonstrates no discernible peak tailing.

quant
Quickly obtain high-quality MS/MS data
Unique collision cell design
Patented LINAC high-pressure collision cell technology used in the API 4000 system ensures maximum ion transfer from the interface to the detector in MS/MS mode. The LINAC collision cell enables fast scanning without compromising performance in all modes of MS/MS operation, including multiple reaction monitoring (MRM), product ion scan, precursor ion scan, and neutral loss scan. For quantitative applications, the advantages of the LINAC collision cell translate into multiple component analysis in a single-period MRM experiment. In qualitative applications, the LINAC collision cell enables faster precursor ion and neutral loss scans with excellent sensitivity and resolutionmaking it the ideal system for Information Dependent Acquisition (IDA) in metabolite identification.

Fast scanning capabilities

Analyst software for quantitation

Windows NT platform-based Analyst software is the industry standard for high-throughput quantitation. Analyst software now facilitates GLP compliance, including 21 CFR Part 11. The softwares core functions enable the user of the API 4000 system to configure, tune, acquire, explore, and quantitate with superior results from any matrix. Analyst software provides: Quantitative Optimizationfor easy creation of fully optimized methods for multiple compounds Protocol Generatorfor rapid batch creation Quantitation Wizardfor simple generation of quantitation methods Slide Showfor quick review of peak integration Metric Plotfor clear visualization of results and trends

Powerful Analyst software

Analyst softwares data acquisition and processing capabilities, combined with increased throughput, provide rapid access to information for DMPK analyses.

Automated, unattended experiments Quick access to information Faster decision-making

itation
Versatile software tools
The automated add-on applications for Analyst software IDA, Metabolite ID, and Automatonextend its functionality. The IDA application maximizes the MS and MS/MS information generated from a single run. Using IDA with triple quadrupole systems, you obtain unprecedented selectivity of candidate ion species when precursor ion and neutral loss scans are used in the decision-making process. You can also exclude ions by using the specific mass and retention time (SMART) filters. The Metabolite ID application automates data acquisition and processing for metabolite profiling. The Automaton application easily performs automated, unattended optimization and quantitative analyses of samples from drug discovery and development experiments. Applied Biosystems continues to create innovations such as the API 4000 system that produce higher-quality data, so you can make better-informed decisions and arrive at discoveries faster. Information Dependent Acquisition (IDA) application
MS survey scan Select candidates based on mass and intensity threshold Are ions excluded by specific mass and retention time filters?

Acquire MS/MS spectrum

IDA enables intelligent, unattended data acquisition and processing based on criteria you establishrapidly acquiring and converting data to information.

from our lab to yours

Applied Biosystems/MDS SCIEX introduced the first dedicated LC/MS/MS system based on innovative IonSpray technology (pneumatically assisted electrospray ionization) in 1989. Today, we maintain the largest installed base of dedicated LC/MS/MS systems in the world. Applied Biosystems/MDS SCIEX systems have revolutionized mass spectrometry by dramatically increasing the range and ease with which compounds are analyzed.

The API 4000 LC/MS/MS System is part of a comprehensive line of superior technology solutions for LC/MS and LC/MS/MS. API QSTAR Pulsar Hybrid LC/MS/MS System

Worldwide Sales Offices

Applied Biosystems vast distribution and service network, composed of highly trained support and applications personnel, reaches into 150 countries on six continents. For international office locations, please call the division headquarters or refer to our Web site at www.appliedbiosystems.com. Applera Corporation is committed to providing the worlds leading technology and information for life scientists. Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses. Applied Biosystems/MDS SCIEX is a joint venture between Applera Corporation and MDS Inc.

API 3000 LC/MS/MS System

API 2000 LC/MS/MS System

Headquarters
850 Lincoln Centre Drive Foster City, CA 94404 USA Phone: 650.638.5800 Toll Free: 800.345.5224 Fax: 650.638.5884 For Research Use Only. Not for use in diagnostic procedures.

API 150EX LC/MS System

AB (Design), Applera, Analyst, API 150EX, API 2000, API 3000, API 4000, Curtain Gas, QSTAR, and Turbo V are trademarks, and Applied Biosystems and TurboIonSpray are registered trademarks of Applera Corporation or its subsidiaries in the US and certain other countries. LINAC, MDS, and SCIEX are trademarks of MDS Inc. Windows NT is a registered trademark of Microsoft Corporation. 2001 Applied Biosystems. All rights reserved. Printed in the USA. 4/16/2001, PJA. Publication 114BR07-01

www.appliedbiosystems.com