Host Lh750

COULTER LH 750 WORKSTATION
Host Transmission Specification
N E C
M u ltiS y n c L C D 1 7 0 0 m +
GEN S
PN 4277303B (April 2002) COULTER CORPORATION A Beckman Coulter Company Miami, Florida 33196-2500 USA
LEGAL NOTICES
READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL BEFORE ATTEMPTING TO OPERATE INSTRUMENT. HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows: WARNING CAUTION IMPORTANT Might cause injury. Might cause damage to the instrument. Might cause misleading results.
CAUTION System integrity might be compromised and operational failures might occur if: r r r This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals. You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your systems computer with software authorized by Beckman Coulter. You install software that is not an original copyrighted version. Only use software that is an original copyrighted version to prevent virus contamination.
Beckman Coulter, Inc. urges its customers to comply with all national health and safety standards such as the use of barrier protection. This may include, but it is not limited to, protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this or any other automated laboratory analyzer.
WARNING Risk of operator injury if all covers are not secured in place prior to instrument operation or you attempt to replace a part without carefully reading the replacement instructions. Do not attempt to replace any component until you carefully read the instructions for replacing the component.
IMPORTANT If you purchased this product from anyone other than Beckman Coulter or an authorized Beckman Coulter distributor, and, if it is not presently under a Beckman Coulter service maintenance agreement, Beckman Coulter cannot guarantee that the product is fitted with the most current mandatory engineering revisions or that you will receive the most current information bulletins concerning the product. If you purchased this product from a third party and would like further information concerning this topic, call your Beckman Coulter Representative.
REVISION STATUS
Initial Issue, 7/01 Software Version 1A. Revision B, 4/02 Software Version 2A. Derived from internal Part Number 0302000G. Updated Section 4.3, Dilution Factor field comments. Removed all references to + Subset globally. Added WBC Threshold Full Histogram Group Fields. Added new Section 3.18, Collate the ToDo List. Updated General Information Group Fields from 29 groups to 24 groups. Added notes to Tables 4-3 and 4-15, respectively. Updated the following tables: 4-1; 4-3; 4-6; 4-8; 4-12; 4-13; 4-21; 4-30; 4-31; 4-33; 4-39; 4-41; 4-46; 4-48; 4-52; 5-1; 5-2; 5-4. Removed NRBC note from Tables 4-24 and 4-46. Added Note: Parameter selection does not affect control transmissions. to Tables 4-43; 4-44; 4-46; 4-48; and 4-50. Updated Control Information Group 34, removed reference WBC Count Field. Updated Section 6.2, added SOH to table. Updated Section 8.1.
This document applies to the latest software listed and higher versions. When a subsequent software version changes the information in this document, a new issue will be released.
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REVISION STATUS
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CONTENTS
LEGAL NOTICES, ii REVISION STATUS, iii 1 INTRODUCTION, 1-1 1.1 2 PURPOSE, 1-1
HARDWARE INTERFACE, 2-1 2.1 HARDWARE HANDSHAKE, 2-1
COMMUNICATION PARAMETERS, 3-1 3.1 3.2 COMMUNICATION PARAMETERS, 3-1 COMMUNICATION PROTOCOLS, 3-1 Low Level Protocol, 3-1 High Level Protocol, 3-1 TIME-OUT, 3-2 BAUD RATE, 3-2 DATA BITS, 3-2 PARITY, 3-2 STOP BITS, 3-2 HANDSHAKE, 3-2 BLOCK SIZE, 3-2
3.3 3.4 3.5 3.6 3.7 3.8 3.9
3.10 GRAPHICS, 3-2 3.11 COMPATIBILITY, 3-3 3.12 SPOOLER, 3-3 3.13 CLEARING THE SPOOLER, 3-3 3.14 REPLACE NULL BY SP, 3-3 3.15 DISABLING HOST COMMUNICATIONS, 3-3 3.16 ENABLE VALIDATION CODES, 3-3 3.17 Replace DLE E with DLE C, 3-4 3.18 Collate the ToDo List, 3-4
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CONTENTS
LH 750 WORKSTATION TO HOST COMMUNICATIONS, 4-1 4.1 DATA-LINK, 4-1 Full Software Handshake, 4-1 Software Handshake Action, 4-1 No Handshake, 4-2 Data Block Structure, 4-2 Group Definition Locator Table, 4-3 1G.1 + RETICS FORMAT, 4-4 Message Structure, 4-4 Preamble, 4-4 Group, 4-4 Field Count, 4-4 Fields, 4-5 Postamble, 4-5 General Information Group Fields, 4-6 CBC Parameter Group Fields, 4-9 DIFF Count Parameter Group Fields, 4-10 DIFF Percent Parameter Group Fields, 4-12 Comment Group Fields, 4-13 Flag Group Fields, 4-14 Demographics Group Fields, 4-17 DF1 DataPlot Group Fields, 4-20 DF2 DataPlot Group Fields, 4-20 DIFF (VCS) Histogram Group Fields, 4-20 RBC Histogram Group Fields, 4-20 PLT Histogram Group Fields, 4-21 RETIC Parameters Group Fields, 4-21 DF5 LS DataPlot Group Fields, 4-24 DF6 OP DataPlot Group Fields, 4-24 RETIC Histogram Group, 4-24
4.2
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CONTENTS
4.3
LH 750 WORKSTATION FORMAT, 4-25 Message Structure, 4-25 Group Definition, 4-28 General Information Group Fields, 4-29 , 4-33 CBC Parameters Group Fields, 4-33 DIFF Count Parameters Group Fields, 4-34 DIFF Percent Parameters Group Fields, 4-36 Comment Group Fields, 4-38 Definitive Flags Group Fields, 4-38 Suspect Flags Group Fields, 4-39 Conditional Flags Group Fields, 4-40 Other Flags Group Fields, 4-42 Demographics, 4-43 RBC Histogram Group Fields, 4-47 PLT Histogram Group Fields, 4-47 RETIC Parameters Group Fields, 4-48 WBC Threshold Group Fields, 4-49 WBC Threshold Full Histogram Group Fields, 4-49 DIFF Latex Parameters Group Fields, 4-50 RETIC Latex Parameters Group Fields, 4-51 Control CBC Parameters Group Fields, 4-55 Control DIFF Count Parameters Group Fields, 4-57 Control DIFF Percent Parameters Group Fields, 4-58 Control RETIC Parameters Group Fields, 4-59 DIFF Graphics, 4-61 Retic Graphics, 4-61 HOST PARSING OF TRANSMITTED DATA, 4-62
4.4 5
HOST TO LH 750 WORKSTATION COMMUNICATIONS (HOST ToDo LIST), 5-1 5.1 DATA-LINK, 5-1 Protocol, 5-1 Data Block Structure, 5-4 PRESENTATION, 5-4 Message Structure, 5-4 DLE Responses From The Workstation And Effects After A ToDo List Download, 5-5 MESSAGE DEFINITION, 5-7 Delete Message, 5-15
5.2
5.3 5.4 6
ASCII Tables, 6-1 6.1 6.2 7-BIT ASCII CODES, 6-1 VALID HOST COMMUNICATIONS ASCII CODES, 6-1
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CONTENTS
CRC, 7-1 7.1 7.2 7.3 CRC ALGORITHM, 7-1 CRC EXAMPLE WRITTEN IN ASM86, 7-2 CRC EXAMPLE WRITTEN IN C, 7-3
GRAPHIC RECONSTRUCTION, 8-1 8.1 8.2 RBC, WBC THRESHOLD, AND PLT HISTOGRAMS, 8-1 LH 750 DATAPLOT FORMAT, 8-1 Dataplots, 8-1 Dataplot Data Header, 8-2 Dataplot Data Header Elements, 8-2 Dataplot Data Block, 8-2 Dataplot Data Block Header, 8-3 Rendering Information Block, 8-3 Rendering Information Block Header, 8-4 Color Palette, 8-4 Dithered Display Bitmap Library, 8-4 Dataplot Header, 8-5
TRADEMARKS
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CONTENTS
ILLUSTRATIONS
8.1 8.2 8.3 8.4 8.5 8.6 8.7 8.8 8.9 8.10 8.11 Plotting RBC, WBC Threshold and PLT Histograms, 8-1 Dataplot Transmission, 8-2 Dataplot Data Header, 8-2 Dataplot Data Block Format, 8-2 Dataplot Data Block Header, 8-3 Rendering Information Block, 8-4 Rendering Information Block Header Format, 8-4 Palette Table Entry, 8-4 Bitmap Format, 8-5 Dataplot Header, 8-5 Dataplot Data Format, 8-6
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CONTENTS
TABLES
3.1 3.2 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 4.10 4.11 4.12 4.13 4.14 4.15 4.16 4.17 4.18 4.19 4.20 4.21 4.22 4.23 4.24 4.25 4.26 4.27 4.28 4.29 4.30 4.31 4.32 4.33 4.34 4.35 4.36 4.37 4.38 4.39 4.40 4.41 4.42 4.43 4.44 4.45 4.46 Communication Parameters Available by Format, 3-1 Graphics Options, 3-3 Group Definition Locator, 4-3 Group Definition: 1G.1 + Retics, 4-5 General Information -- Group 1, 4-6 CBC Parameters -- Group 2, 4-9 Flag Data for CBC Parameter Group Fields, 4-10 DIFF Count Parameters -- Group 3, 4-10 Flag Data for Diff Count Parameter Group Fields, 4-11 DIFF Percent Parameters -- Group 4, 4-12 Flag Data for DIFF Percent Parameter Group Fields, 4-13 Comment -- Group 5, 4-13 Flag -- Group 6, 4-14 LH 750 Workstation/STKS 1G.1 + Retics Messages, 4-14 Demographics -- Group 7, 4-18 RBC Histogram -- Group 11, 4-20 PLT Histogram -- Group 12, 4-21 RETIC Parameters -- Group 13, 4-21 Flag Data for Retic Parameter Group Fields, 4-23 Transmission Tests and Test IDs by Test Type, 4-25 Transmitted Groups, 4-28 General Information -- Group 1, 4-29 Possible Values for Sample Status, 4-32 CBC Parameters -- Group 2, 4-33 Flag Data for CBC Parameter Group Fields, 4-34 DIFF Count Parameters -- Group 3, 4-34 Flag Data for DIFF Count Parameter Group Fields, 4-36 DIFF Percent Parameters -- Group 4, 4-36 Flag Data for DIFF Percent Parameter Group Fields, 4-37 Comment -- Group 5, 4-38 Definitive Flags -- Group 6, 4-38 Suspect Flags -- Group 7, 4-39 Conditional Flags -- Group 8, 4-40 Other Flags -- Group 9, 4-42 Demographics -- Group 10, 4-43 RBC Histogram -- Group 14, 4-47 PLT Histogram -- Group 15, 4-47 RETIC Parameters -- Group 16, 4-48 Flag Data for Retic Parameter Group Fields, 4-49 WBC Threshold Monitor Group 26, 4-49 DIFF Latex Parameters -- Group 31, 4-50 Flag Data for DIFF Latex Parameters Group Fields, 4-51 RETIC Latex Parameters -- Group 32, 4-51 Flag Data for RETIC Latex Parameters Group Fields, 4-52 Control Information -- Group 34, 4-52 Control CBC Parameters -- Group 35, 4-55 Flag Data for Control CBC Parameters Group Fields, 4-57 Control DIFF Count Parameters -- Group 36, 4-57
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CONTENTS
4.47 4.48 4.49 4.50 4.51 4.52 4.53 5.1 5.2 5.3 5.4
Flag Data for DIFF Count Parameter Group Fields, 4-58 Control DIFF Percent Parameters -- Group 37, 4-58 Flag Data for Diff Percent Parameter Group Fields, 4-59 Control RETIC Parameters -- Group 38, 4-60 Flag Data for Control RETIC Parameter Group Fields, 4-61 DIFF DataPlot -- Group 50, 4-61 Retic DataPlot -- Group 51, 4-61 Message Definition Table, 5-7 Possible Values and Examples of Test Requests, 5-14 Possible Combinations of Sample Requests with Multiple Test Requests, 5-14 Patient ID Deletions, 5-17
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1INTRODUCTION 1
1.1 PURPOSE
This document is the specification for communication between the LH 750 Workstation and a host computer. The functionality will remain backward compatible. This document is intended for use by software engineers needing to either maintain or modify the operation of the LH 750 Workstation Host Communications. This document describes the transfer of data from the LH 750 Workstation to an LIS/HIS computer in the following formats: r r STKS 1G.1 + Retics LH 750 Workstation: host communication protocol which supports transmission of CBC, DIFF and Reticulocytes parameters for samples and control runs as well as collated sample runs. This format is an extension of the STKS 2A and 3A formats.
The LH 750 STKS 1G.1 + Retics format is provided for use with existing STKS and MAXM interfaces. Due to the LH 750 Workstations new features and enhancements, STKS and MAXM instrument formats are not completely supported (see details in following sections).
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INTRODUCTION PURPOSE
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2HARDWARE INTERFACE 2
The system is equipped with a Host 1 connector on the rear of the LH 750 Workstation that lets the system interface with a host computer via a Standard EIA-type 25-pin connector and uses EIA Standard RS-232-C signals. This is a Data Terminal Equipment (DTE) configuration. The LH 750 Workstation/Host communications uses only the following signals: P3 Pin No. 2 3 4 5 6 7 8 20 22 Signal Name Transmit Data Received Data Ready To Send (RTS) Clear To Send (CTS) Data Set Ready (DSR) Signal Ground Carrier Detect Data Terminal Ready (DTR) Ring Indicator Flow Direction From LH 750 Workstation To LH 750 Workstation From LH 750 Workstation To LH 750 Workstation To LH 750 Workstation Reference Reference From LH 750 Workstation Reference
The LH 750 Workstation is the DTE, by RS-232 standards, which explains the RTS/DTR pin-outs. Whether the HOST is a DTE or DCE will determine its use of CTS/DSR or RTS/DTR. A DTE HOST requires the use of a NULL MODEM Cable. Note: Pin number 5 CTS must be active for LH 750 Workstation to send any transmissions. If the host computer does not support the above hardware handshake lines, it will be necessary to connect pin 4 to pin 5 and to connect pin 20 to pin 6.
2.1
HARDWARE HANDSHAKE
During a transmission, the host logically raises CTS to allow the LH 750 Workstation to send data and logically lowers CTS to prevent the LH 750 Workstation from sending data. Note: If CTS is lowered to hold off the LH 750 Workstation host transmission, it must be raised again within the LH 750 Workstation time-out period. If CTS is not raised within the time-out period, the LH 750 Workstation transmission to the host will time-out and reset, ready for the next transmission.
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HARDWARE INTERFACE HARDWARE HANDSHAKE
Diagram of Hardware Handshake Mode

LH 750 Workstation (SENDER) send data -----> send data -----> send data -----> <-- CTS off (stop sending data) wait <-- CTS on (send more data) send data -----> send data -----> - etc.process received data Host (RECEIVER) <-- CTS on (send me data)
2-2
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3COMMUNICATION PARAMETERS 3
3.1 COMMUNICATION PARAMETERS
The LH 750 Workstation allows the user to configure the following communication parameters on the Host Computer Definition screen. These parameters include communication modes, as well as parameters enabling the transmission of graphic data.
3.2
COMMUNICATION PROTOCOLS
The transmission specification consists of data-link (low level) and presentation (high level) protocols. Data-link protocol provides the means of transmitting data without any regard to actual information. Presentation protocol describes the actual information and its format.
Low Level Protocol

The Host Low Level Communication Protocol will be supported by all formats.
High Level Protocol

The High Level Communication Protocols currently supported are the STKS 1G.1 + Retics and LH 750 Workstation. The High Level Communication Protocol is selected using the compatibility field. Note: To satisfy the host communication requirements, the receiver MUST parse for all of the data.
DO NOT ASSUME FIXED OFFSETS

Table 3.1 lists the communication parameters available by format.
Table 3.1 Communication Parameters Available by Format Communication Parameter Time-out Baud Rate Data Bits Parity Stop Bits Handshake Block Size Graphics Compatibility 1G.1 + Retics X X X X X X X X X LH 750 Workstation X X X X X X X X X
X in 1G.1 + Retics column = Supported by the LH 750 STKS 1G.1 + Retics format. X in LH 750 Workstation column = Supported by the LH 750 Workstation format.
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COMMUNICATION PARAMETERS TIME-OUT
3.3
TIME-OUT
The time-out value determines the amount of time the LH 750 Workstation waits for a response from the host before retrying to send data to the host. The LH 750S Workstation continues to transmit at the time-out intervals until the host successfully receives the data. The time-out values are 30, 60, and 90 seconds. The default value is 90 seconds.
3.4
BAUD RATE
The following baud rates are supported: 2400, 4800, 9600, 19200 and 38400. Note: The minimum recommended baud rate is 2400 for non-graphic transmissions and 9600 Baud for graphic transmissions.
3.5 3.6 3.7 3.8
DATA BITS
Host Communication can be configured for 7 or 8 bit mode.
PARITY
Odd, Even, and Off (None) parity modes are supported. Odd parity is the default value.
STOP BITS
None, 1, or 2 Stop Bit modes are supported. The default value is 2.
HANDSHAKE
This option determines whether or not the LH 750 Workstation looks for a handshake from the host computer. See Heading 4.1, Data-Link, for additional information.
3.9
BLOCK SIZE
The LH 750 Workstation/Host Communications support block sizes of 128 and 256 bytes. The default value is 256 bytes.
3.10
GRAPHICS
The LH 750 Workstation allows DIFF and Retic graphic transmission if the compatibility format selected is the LH 750 Workstation format. Table 3.2 lists the graphic items that can be enabled or disabled for the corresponding test based on the current compatibility format and report profile selected.
3-2
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COMMUNICATION PARAMETERS COMPATIBILITY
Table 3.2 Graphics Options Test CBC Graphics RBC Histogram PLT Histogram WBC Threshold DIFF RETICS Subset DataPlot DataPlot DataPlot 1G.1 + Retics X X N/A N/A N/A N/A LH 750 Workstation X X X X X X
X in 1G.1 + Retics column = Supported by the LH 750 STKS 1G.1 + Retics format. X in LH 750 Workstation column = Supported by the LH 750 Workstation format.
3.11
COMPATIBILITY
This option determines which transmission format is used by the LH 750 Workstation to send results to an LIS/HIS computer. The Host High Level Communication Protocols supported are: STKS 1G.1 + Retics and LH 750 Workstation.
3.12
SPOOLER
Transmissions to the Host are spooled before sending. When a transmission is sent to the Host, it will be kept on the Spooler and resent until the Host acknowledges that the transmission was successfully received. If the Host is offline when Transmit To Host is enabled, all transmissions intended for the Host will be kept on the Spooler until the Host comes online or the Host Spooler is cleared.
3.13
CLEARING THE SPOOLER

Select the Clear button in the LIS folder on the System Run Configuration screen to clear all spooled transmissions intended for the Host. It should be remembered that new transmissions to the Host will still be subsequently spooled. See Section 3.15 to disable host communications and hence the host spooler.
3.14
REPLACE NULL BY SP
This is no longer an option because NULL characters are not included in the transmission buffer.
3.15
DISABLING HOST COMMUNICATIONS

Select the Disable button in the LIS folder on the System Run Configuration screen to disable all auto transmissions from the LH 750 Workstation to the Host. This will not affect manual transmissions or transmissions from the Host to the LH 750 Workstation.
3.16
ENABLE VALIDATION CODES

This checkbox enables the codes used to identify the validation status of individual test requests on a sample as detailed in Conditional Flags Group Fields under AutoValidation in Chapter 4. If Enable Validation Codes in System Setup - [Communications] is checked (default), the individual test group validation status will be transmitted. Applied only to LH 750 format.
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3-3
COMMUNICATION PARAMETERS Replace DLE E with DLE C
3.17
Replace DLE E with DLE C

Select this checkbox to replace DLE E with DLE C in the standard data set transmitted to your information system.
3.18
Collate the ToDo List

The ToDo List generation is derived from task pre-assignment, by determining what sets of data are to be collated into a final report, test panels are pre-assigned either manually or by LIS download.
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4LH 750 WORKSTATION TO HOST COMMUNICATIONS 4

4.1 DATA-LINK
A sample transmission to the Host consists of one or more framed data blocks. See Data Block Structure below. All transmitted data bytes are ASCII characters. All numeric values are ASCII representations of hexadecimal numbers. Hence, for example, the number FF (hex) is represented by the two ASCII bytes "FF" (46H, 46H). The first two bytes of the transmission are the number of blocks to be sent, in ASCII, followed by transmission of data blocks (see format below). The final block will be padded with ASCII spaces (20H) to fill a whole block. The LH 750 Workstation supports full software handshake and no software handshake protocols.
Full Software Handshake

The LH 750 Workstation sends the following control characters plus data and expects the indicated host response:
LH 750 Workstation (SENDER) SYN (ready)------> <-------SYN (go ahead) Block Count------> <-------ACK (ready to receive) or NAK (receiver abort) send data blocks Block ------> for each block SYN (all done)------> <-------ACK (transmission accepted) <-------ACK (block received ok) or NAK (retransmit this block) or SYN (retransmit all blocks) HOST (RECEIVER)
Up to 255 blocks of data can be sent. The actual number of blocks sent is specified by the two-byte ASCII Block Count.
Software Handshake Action

If the host NAKs a data block, the block is retransmitted. It is up to the host to determine how many times it retries receiving a NAKed block before aborting. Typically, the Host will NAK a datablock if the current block number is incorrect(e.g. out of sequence), the Hosts calculated CRC does not match the transmitted blocks CRC or block framing is not recognized as correct. A SYN sent by the Host after a block has been transmitted by the LH 750 Workstation forces the Workstation to retransmit all data starting with the first block. This does not include the
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LH 750 WORKSTATION TO HOST COMMUNICATIONS DATA-LINK
initial SYN and block count. It is up to the Host to determine under what conditions to transmit a SYN. The LH 750 Workstation continues to send the same sample transmission until the Host accepts it entirely (ACKs each block). To abort a transmission, the host must ACK each block of the transmission and discard it locally.
No Handshake
If Handshake option is disabled, the protocol ignores all hardware and software host hanshake responses. In addition, in the No Handshake mode, the LH 750 Workstation does not send a SYN prior to transmitting the data. CTS is always used to indicate that a Host is MS and turned on.
Data Block Structure
Byte # 1 2 3 4 STX BLK NBR MS CHAR BLK NBR LS CHAR DATA BYTES 259 (131) 260 (132) 261 (133) 262 (134) 263 (135) 264 (136) CRC MSB MS CHAR CRC MSB LS CHAR CRC LSB MS CHAR CRC LSB LS CHAR ETX
# of bytes 1 byte 1 byte 1 byte 256 (128) bytes C R C
1 byte 1 byte 1 byte 1 byte 1 byte
Each data block is framed with a Start Of Text(STX = 02H) and an End Of Text(ETX =03H) control character. The next two bytes after STX have the Current Block Sequence Number(BLK NBR) in ASCII starting with the number 1(MS byte = 30H, LS byte = 31H, to give 01) for the first block. The block number of the last block must equal the Block Count sent at the beginning of the transmission. Every data block will have either 128 or 256 bytes (whichever is chosen). If there is insufficient data to fill a block, it will be padded with space characters (last block transmitted). The CRC for the blocks data is calculated by the workstation and sent as the last four bytes of the block before the ETX in the ASCII format shown above. The Most Significant Byte (MSB) is generated as a Most Significant and Least Significant ASCII character (MS and LS CHAR). The same is true for the Least Significant Byte (LSB). 4-2
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LH 750 WORKSTATION TO HOST COMMUNICATIONS DATA-LINK
The algorithm used to calculate the CRC for each block is a modified CCITT CRC16. CRC is only calculated on the data bytes. Chapter 7 details the CRC algorithm and includes application notes for implementing the algorithm in both C and assembly language.
Group Definition Locator Table

Table 4.1 lists the group names for each format.
Table 4.1 Group Definition Locator 1G.1 + Retics, Heading 4.2
X X X X X
Group Name
General Information CBC Parameters DIFF Count Parameters DIFF Percent Parameters Comment Definitive Flags Suspect Flags Conditional Flags Other Flags Flags Demographics DF1 DataPlot DF2 DataPlot DIFF (VCS) Histogram RBC Histogram PLT Histogram RETICS Parameters DF5 LS DataPlot DF6 OP DataPlot RETICS Histogram WBC Threshold WBC Threshold Full Histogram DIFF Latex Parameters RETIC Latex Parameters Control Information Control CBC Parameters Control DIFF Count Parameters Control DIFF Percent Parameters Control RETICS Parameters DIFF graphics Retic graphics
Group Number
1 2 3 4 5
Group Number
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 26 27 31 32 34 35 36 37 38 50 51
LH 750 Workstation, Heading 4.3

X X X X X X X X X X
6 7 8 9 10 11 12 13 14 15 16
X X X X X X X X X X X
X X X
X X X X X X X X X X X
X in 1G.1 + Retics column = Described under Heading 4.2, STKS 1G.1 + Retics Format. X in LH 750 Workstation column = Described under Heading 4.3, LH 750 Workstation Format.
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LH 750 WORKSTATION TO HOST COMMUNICATIONS 1G.1 + RETICS FORMAT
4.2
1G.1 + RETICS FORMAT

Message Structure
The Presentation deals with the high level format of the message. The data bytes of the transmission blocks, when collected together, exhibit the following high level format.
PREAMBLE GROUP x1 GROUP xn POSTAMBLE
Preamble The preamble marks the beginning of a message.

C L R F C L C L C L C L C L R F R F R F R F R F C L R F
Group
D FIELD C L C COUNT R F 1
FIELD 1
C L R F
FIELD N
C L R F
The DC1 (11H) character marks the beginning of a Group. There is no relationship between the order in which the groups are defined in the following sections and the order in which they appear after the preamble. Groups will never be omitted. Each Group contains a DC1 (11H) character, marking the beginning of the section, a two character field count and a variable number of fields each ending with a CR (0DH) and an LF (0AH). Note: When there are no data for a Group, the Group will appear as follows:
D C 0 1 C L 0 R F
Field Count Each Group has a Field Count which identifies the number of fields contained within the Group. Valid field counts can be from 0-255. The field count uses two characters to give the ASCII representation of the hexadecimal value.
MS CHAR LS CHAR Two-byte ASCII representation of Hex value between 00 and FF (0 to 255)
The field count will reflect the total number of fields transmitted within the Group. The field count will always be the sum of CR/LF pairs within the Group minus one.
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Fields Each Group is made up of variable length fields which may or may not be padded with SP (20H) characters. A field may have a variable length tag preceding the data separated with one or more SP (20H) characters. The data portion of a field will only contain ASCII characters in the range of 20H to 7EH inclusive. Chapter 6 tabulates the valid ASCII characters. Fields are separated by a CR (0DH) and an LF (0AH) character. Note: A field's position within a Group must not be used to identify it. Fields within a Group may appear in any order or may be omitted. The CR/LF should be used to find a field and the Tag used to identify it. The syntax for the Data Format of a field is: A - Alpha characters N - Numeric characters X - Printable characters Sep - Separator SP (a..z and A..Z and space) or (61H..7AH and 41H..5AH and 20H) (0..9 and + - .) or (30H..39H and 2BH 2DH 2EH) (20H..7EH) one or more SP characters Space (ASCII 20H). Please note that all field transmissions will include a SP between the tag and data format (this will be implied in the following sections), as illustrated below:
Tag
T I M E
Sep
Data Format
N N : N N : N N
Postamble
D C C R 1 L C L F R F C L R F
The postamble marks the end of the current message. There are 16 groups defined in the current implementation of the STKS 1G.1 + Retics format. Future revisions may include additional groups. Any future additions will not disrupt the numbering of the groups as presently defined. Group Definition Table 4.2 lists the groups supported by the 1G.1 + Retics format.
Table 4.2 Group Definition: 1G.1 + Retics Group Number 1 2 3 4 1G.1 +Retics + Subsets X X X X
Group Name General Information CBC Parameters DIFF Count Parameters DIFF Percent Parameters
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4-5
Table 4.2 Group Definition: 1G.1 + Retics (Continued) 5 6 7 8 9 10 11 12 13 14 15 16 Comments Flags Demographics DF1 DataPlot DF2 DataPlot DIFF (VCS) Histogram RBC Histogram PLT Histogram Retic Parameter DF5 DataPlot DF6 DataPlot Retic Histogram X X X * * * X X X * * *
X = Supported by the LH 750, STKS 1G.1 + Retics format. * = Not supported the LH 750 STKS 1G.1 + Retics format. The Group is transmitted with a field count of 00.
General Information Group Fields Table 4.3 identifies the field names, test types, and applicable format of General Information Group transmissions.
Table 4.3 General Information -- Group 1 Field Name Run Date Run Time ID #1 ID #2 Cassette/Position Sequence Number 1E Cassette/Position ID #1 Status Cassette/Position Status ToDo List Status Test Type C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R 1G.1 + Retics X X X X X X X X X X
X = Supported by the LH 750, STKS 1G.1 + Retics format.
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Run Date Field: Tag

D A T E
Data Format
N N / N N / N N
Data Length
9 bytes
Comments
Date output is month/day/year, regardless of the system date configuration.
Run Time Field: Tag

T I M E
Data Format
N N : N N : N N
Data Length
12 bytes
Comments
Time output is on 24 hours, regardless of the system time configuration. Time ouput is hours:minutes:seconds..
ID #1 Field: Tag
I D 1
Data Format
X X X X X X X X
Data Length
16 bytes
Comments
SAMPLE ID
ID #2 Field: Tag
I D 2
Data Format
X X X X X X X X
Data Length
16 bytes
Comments
Concatenation of Patient last and first name separated by a comma and a space. Only the first 16 characters of the concatenated string are transmitted. Example: Doe, John.
Cassette/Position Field: Tag

C A S S / P O S
Data Format
X X X X / X X
Data Length
8 bytes
Comments
CASS/POS output is cassette number/cassette position. SECONDA - Manual sampling mode.
P/N 4277303B
4-7
Sequence Number: Tag

S E Q U E N C E
Data Format
X X X X X X
Data Length
7 bytes
Comments
Sequence Number
1E Cassette/Position Field: Tag

1 E C / P
Data Format
A X X X X X X
Data Length
8 bytes
Comments
Date Values: S - Manual sampling mode - Automatic sampling mode.
ID #1 Status Field: Tag

I D 1 S T A T U S
Data Format
A
Data Length
2 bytes
Comments
Data Values: P - positive ID. E - edited ID. - not edited and not positive ID. N - Auto-numbered. Precedence order is E, N, P and .
Cassette/Position Status Field: Tag

C / P S T A T U S
Data Format
A
Data Length
2 bytes
Comments
Data Values: P - positive ID. E - edited ID. - not edited and not positive ID. Precedence order is: E, P and .
ToDo List Status: Tag

W L S T A T U S
Data Format
A A A A A A A A
Data Length
9 bytes
Comments
See note below.*
*Data Values: GOOD = PART ASP = NO READ =
O.K. (Complete) Partial aspiration Bar code not read
4-8
P/N 4277303B
NO MATCH = (20H) =
Match not found in ToDo list Primary ID is cassette position and sample ID does not match preassigned ID
CBC Parameter Group Fields Table 4.4 identifies the field names, test types, and applicable format of CBC Parameter Group transmissions
Table 4.4 CBC Parameters -- Group 2 Field Name WBC RBC HGB HCT MCV MCH MCHC RDW PLT PCT MPV PDW Test Type C, CD, CDR, CR, Edited C, CD, CDR, CR, R , Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited 1G.1 + Retics X X X X X X X X X X X X
X = Supported by the LH 750, STKS 1G.1 + Retics format. For Retic only samples, only the RBC parameter is applicable. If the RBC parameter value is not available, then it will not be transmitted and this group will have a field count of 00.
Each field in the CBC Parameters Group will have the following format: Tag
A A A A
Data Format
X X X X X X A A A
Data Length
See Data Format column.
Comments
See Below.**
6 char numeric data max.
3 char flag data max.
Result Format:** The parameter result will be transmitted in the Reporting Units with the extra digit criteria selected on the LH 750 Workstation. Numeric Data: ** If the numeric data of each format (for example, xx.xx) does not contain a decimal number, then it contains one of the following: Flag Data: **
P/N 4277303B
4-9
Result
Comments Incomplete computation Parameter overrange Parameter voteout
..... +++++ -----
The flag data characters for the CBC Parameter Group Fields are as follows in Table 4.5. Table 4.5 lists all the possible flags generated on the LH 750 Workstation and how they translate to the STKS 1G.1 + Retics format.
Table 4.5 Flag Data for CBC Parameter Group Fields STKS 1G.1 Formats Translation (3 characters) R H L H L H L E e * R R
Comments Review parameter. Exceeds reference interval high limit Exceeds reference interval low limit Exceeds action high limit Exceeds action low limit Exceeds critical high limit Exceeds critical low limit Operator edited result Recalculated result from edited result MCV threshold failure Delta check failed Partial aspiration/blood detector disabled Result exceeds linearity *
= Not supported by the STKS 1G.1 + Retics format.
DIFF Count Parameter Group Fields Table 4.6 identifies the field names, test types, and applicable format of DIFF Count Parameter Group transmissions.
Table 4.6 DIFF Count Parameters -- Group 3 Field Name LY# MO# NE# EO# BA# NRBC# Test Type CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited
X = Supported by the STKS 1G.1 + Retics format.
1G.1 + Retics X X X X X
4-10
P/N 4277303B
This group is applicable to CBC/DIFF and CBC/DIFF/Retic sample runs. For all other sample runs, this group will have a field count of 00. Each field in the DIFF Count Parameters Group will have the following format: Tag
A A A A
Data Format
X X X X X X A A A
Data Length
Comments
See Below.**
Result Format: ** The parameter result will be transmitted in the Reporting Units with the extra digit criteria selected on the LH 750 Workstation. Numeric Data: ** If the numeric data of each format (for example, xx.xx) does not contain a decimal number, then it contains one of the following:
Result Comments Incomplete computation Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2) Invalid data: could not calculate DIFF#. WBC parameter overrange or sum of DIFF# parameters is greater than 500.0 x 106 cells/L (whole blood) or sum of DIFF# parameters is greater than 600.0 x 106 cells/L (predilute).
..... :::::
?????
+++++
Flag Data: ** The flag data characters for the DIFF Count Parameter Group fields are as follows in Table 4.7:
Table 4.7 Flag Data for Diff Count Parameter Group Fields STKS 1G.1 Formats Translation (3 characters) R
Comments Review parameter: WBC threshold failure or WBC exceeds linearity or sum of DIFF% results exceeds 100. Exceeds reference interval high limit Exceeds reference interval low limit Exceeds action high limit Exceeds action low limit Exceeds critical high limit
P/N 4277303B
H L H L H
4-11
Table 4.7 Flag Data for Diff Count Parameter Group Fields (Continued) Exceeds critical low limit Operator edited result Recalculated result from edited result Parameter value exceeds Delta check limits Partial aspiration/blood detector disabled Result exceeds linearity R L E e
DIFF Percent Parameter Group Fields Table 4.8 identifies the field names, test types, and applicable format of DIFF Percent Parameter Group transmissions.
Table 4.8 DIFF Percent Parameters -- Group 4 Field Name LY% MO% NE% EO% BA% NRBC% Test Type CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited
1G.1 + Retics X X X X X
This group is applicable to CBC/DIFF and CBC/DIFF/Retics sample runs. For all other sample runs, this group will have a field count of 00. Each field in the DIFF Count Parameters Group will have the following format: Tag
A A A A
Data Format
X X X X X X A A A
Data Length
Comments
See Below.**
Result Format:** The parameter result will be transmitted in the Reporting Units with the extra digit criteria selected on the LH 750 Workstation.
4-12
P/N 4277303B
Numeric Data: ** If the numeric data of each format (for example, xx.xx) does not contain a decimal number, then it contains one of the following:
Result Comments Incomplete computation Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2) Sum of DIFF% parameter results is not equal to 100% +0.1, or DIFF# parameters is greater than 600.0 x 106 cells/L (predilute).
..... :::::
+++++
Flag Data: ** The flag data characters for the DIFF Percent Parameter Group fields will be as follows in Table 4.9:
Table 4.9 Flag Data for DIFF Percent Parameter Group Fields STKS 1G.1 Formats Translation (3 characters) R H L H L H L E e R
Comments Review parameter Exceeds reference interval high limit Exceeds reference interval low limit Exceeds action high limit Exceeds action low limit Exceeds critical high limit Exceeds critical low limit Operator edited result Recalculated result from edited result Delta check failed Partial aspiration/blood detector disabled Result exceeds linearity
Comment Group Fields Table 4.10 identifies the field names, test types, and applicable format of Comment Group transmissions.
Table 4.10 Comment -- Group 5 Field Name Comment Test Type C, CD, CDR, CR, R 1G.1 + Retics X
.
P/N 4277303B
4-13
The Comment Group can have a variable number of fields which consist of a variable number of bytes. Each Comment field is made up of an ASCII string identifying the appropriate flag. Data Format
X X X X X X X X X X X X X X X
Data Length
64 bytes maximum
Comments
Flag Group Fields Table 4.11 identifies the field names, test types, and applicable format of Flag Group transmissions.
Table 4.11 Flag -- Group 6 Field Name Variable Test Type C, CD, CDR, CR, R 1G.1 + Retics X
Note: This group will always be transmitted and will only be sent once per sample transmission. The Flag Group can have a variable number of fields that consist of a variable number of bytes. There are no Tags associated with a Flag field. Each Flag field is made up of an ASCII string identifying the appropriate flag. Data Format
X X X X X X X X X X X X X X X
Data Length
Variable
Comments
Table 4.12 lists all the possible flags generated on the LH 750 Workstation and how they translate to the STKS 1G.1 + Retics format. For example, a Low Event # suspect flag message in LH 750 format is a Review message and an Abnormal WBC Pop message in STKS 1G.1 + Retics format.
Table 4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages LH 750 Workstation Message STKS 1G.1 + Retics Suspect Flags Low Event # Verify Diff Ne Blasts Ly Blasts Mo Blasts Imm. NE 1 Imm. NE 2 Variant LY Review Slide Review Slide Blasts Blasts Blasts Imm Grans/Bands 1 Imm Grans/Bands 2 Variant Lymphs Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop
4-14
P/N 4277303B
Table 4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages (Continued) LH 750 Workstation Message NRBC WBC Interference RBC Interference Dimorphic Reds Red Cell Agglutination Verify Retic Abnormal Retic Pattern Platelet Clumps Giant Platelets Inconsistent Diff CD Abnormal Subsets WBC Exceeds Linearity STKS 1G.1 + Retics NRBCs (N/A) (N/A) Dimorphic RBC Pop RBC Agglutination (N/A) (N/A) Platelet Clumps Giant Platelets (N/A) Abnormal Subsets (N/A) Definitive Flags Leukopenia Leukocytosis Neutropenia % Neutropenia # Neutrophilia % Neutrophilia # Lymphopenia % Lymphopenia # Lymphocytosis % Lymphocytosis # Monocytosis % Monocytosis # Eosinophilia % Eosinophilia # Basophilia % Basophilia # Anemia Anisocytosis 1+ Anisocytosis 2+ Anisocytosis 3+ Anisocytosis Microcytosis Leukopenia Leukocytosis Neutropenia % Neutropenia # Neutrophilia % Neutrophilia # Lymphopenia % Lymphopenia # Lymphocytosis % Lymphocytosis # Monocytosis % Monocytosis # Eosinophilia % Eosinophilia # Basophilia % Basophilia # Anemia Anisocytosis 1+ Anisocytosis 2+ Anisocytosis 3+ Anisocytosis Microcytosis Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal WBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop (N/A) (N/A) Abnormal RBC Pop Abnormal RBC Pop Verify Retic (N/A) Abnormal PLT Pop Abnormal PLT Pop (N/A) (N/A) (N/A)
P/N 4277303B
4-15
Table 4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages (Continued) LH 750 Workstation Message 1+ Microcytosis 2+ Microcytosis 3+ Microcytosis Macrocytosis 1+ Macrocytosis 2+ Macrocytosis 3+ Macrocytosis Hypochromia 1+ Hypochromia 2+ Hypochromia 3+ Hypochromia (N/A) (N/A) (N/A) Erythrocytosis Pancytopenia STKS 1G.1 + Retics 1+ Microcytosis 2+ Microcytosis 3+ Microcytosis Macrocytosis 1+ Macrocytosis 2+ Macrocytosis 3+ Macrocytosis Hypochromia 1+ Hypochromia 2+ Hypochromia 3+ Hypochromia 1+ Poikilocytosis 2+ Poikilocytosis 3+ Poikilocytosis Erythrocytosis Pancytopenia Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal RBC Pop Abnormal WBC Pop and Abnormal RBC Pop and Abnormal PLT Pop (N/A) Abnormal PLT Pop Abnormal PLT Pop Abnormal PLT Pop Abnormal PLT Pop (N/A) Parameter Flags
Reticulocytosis Thrombocytopenia Thrombocytosis Small Platelets Large Platelets H&H Check Failed
(N/A) Thrombocytopenia Thrombocytosis Small Platelets Large Platelets (N/A)
.....
.....
Abnormal WBC Pop and/or Abnormal RBC Pop and/or Abnormal PLT Pop and/or Verify Retic Abnormal WBC Pop and/or Abnormal RBC Pop and/or Abnormal PLT Pop Abnormal WBC Pop and/or Abnormal RBC Pop and/or Abnormal PLT Pop and/or Verify Retic
+++++
+++++
-----
-----
4-16
P/N 4277303B
Table 4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages (Continued) LH 750 Workstation Message ????? STKS 1G.1 + Retics ????? Abnormal WBC Pop and/or Abnormal RBC Pop and/or Abnormal PLT Pop and/or Verify Retic Abnormal WBC Pop and/or Abnormal RBC Pop and/or Abnormal PLT Pop and/or Verify Retic (N/A) (N/A) (N/A) Edited Data Edited Data (N/A) Abnormal WBC Pop and/or Abnormal RBC Pop and/or Abnormal PLT Pop and/or Verify Retic Other Flags Edited Data Preliminary Report Additional Test Report Incomplete Collation SYSTEM ALARM Completed Report Blood Detector Disabled Sample validated Sample not validated AutoValidated Edited Data Preliminary Report (N/A) Collate Failed (N/A) (N/A) (N/A) (N/A) (N/A) (N/A) STKS Messages PCT IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES PDW IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES (N/A) (N/A) (N/A) (N/A) (N/A) (N/A) (N/A) (N/A) (N/A) (N/A)
:::::
:::::
R H L E e D +
R H L E e (N/A) +++++
Note: These flags will be produced only for the research parameters that are transmitted. Demographics Group Fields Table 4.13 identifies the field names, test types, and applicable format of Demographics Group transmissions.
P/N 4277303B
4-17
Table 4.13 Demographics -- Group 7 Field Name Date of Birth User Field #1 User Field #2 User Field #3 Sex Location Physician User Date User Time Test Type C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R 1G.1 + Retics X X X X X X X X X
Note: This group will be transmitted only once per sample transmission. Date of Birth Field: Tag
B I R T H
Data Format
N N / N N / N N N N
Data Length
11 bytes
Comments
Date of birth output is month/day/year, regardless of the system date configuration. The Date Of Birth Year is Always 4 Characters.
User Field #1: Tag

U F 1
Data Format
X X X X X
Data Length
17 bytes
Comments
User Field #2: Tag

U F 2
Data Format
X X X X X
Data Length
17 bytes
Comments
4-18
P/N 4277303B
User Field #3: Tag

U F 3
Data Format
X X X X X
Data Length
17 bytes
Comments
Sex Field: Tag

S E X
Data Format
A
Data Length
2 bytes
Comments
Data Value: M Male F Female (20H) Unknown
Location Field: Tag

L O C A T I O N
Data Format
X X X X X X
Data Length
17 bytes
Comments
Physician Field: Tag

P H Y S I C I A N
Data Format
X X X X X X
Data Length
22 bytes
Comments
Concatenation of Physician last and first name separated by a comma and a space. Only the first 22 characters of the concatenated string are transmitted. Example: Smith, Peter.
User Date Field: Tag

U D A T E
Data Format
N N / N N / N N
Data Length
9 bytes
Comments
Sample Drawn Date. Output format is month/day/year, regardless of the system date configuration.
P/N 4277303B
4-19
User Time Field: Tag

U T I M E
Data Format
N N : N N : N N
Data Length
9 bytes
Comments
Sample Drawn Time. Output is on 24 hours, regardless of the system time configuration. Time output is hours:minutes: seconds.
DF1 DataPlot Group Fields The 1G.1 + Retics format does not support transmission of this group. This group will be transmitted with field count of 00. DF2 DataPlot Group Fields The 1G.1 + Retics format does not support transmission of this group. This group will be transmitted with field count of 00. DIFF (VCS) Histogram Group Fields The 1G.1 + Retics format does not support transmission of this group. This group will be transmitted with field count of 00. RBC Histogram Group Fields Table 4.14 identifies the field names, test types, and applicable format of RBC Histogram Group Field transmissions.
Table 4.14 RBC Histogram -- Group 11 Field Name RBC Histogram Test Type C, CD, CDR, CR 1G.1 + Retics X
If RBC histogram graphic transmission is disabled, this group is transmitted with a field of 00. RBC Histogram Field: Tag
R B C H
Data Format
H H H H H H
Data Length
512 bytes
Comments
See Chapter 8 for graphic reconstruction.
4-20
P/N 4277303B
PLT Histogram Group Fields Table 4.15 identifies the field names, test types, and applicable format of PLT Histogram Group Field transmissions.
Table 4.15 PLT Histogram -- Group 12 Field Name PLT Histogram PLT Fit Histogram Test Type C, CD, CDR, CR C, CD, CDR, CR 1G.1 + Retics X X
If PLT histogram graphic transmission is disabled, this group is transmitted with a field of 00. Note: When the PLT histogram graphic transmission is enabled the data in the PLTF field is always sent. Ignore the data sent in the PLTF field if an R flag is present for the PLT parameter sent in the CBC Parameter Group. PLT Histogram Field: Tag
P L T H
Data Format
H H H H H H
Data Length
256 bytes
Comments
PLT Fit Histogram Field: Tag

P L T F
Data Format
H H H H H H
Data Length
512 bytes
Comments
RETIC Parameters Group Fields Table 4.16 identifies the field names, test types, and applicable format of RETIC Parameters Group Field transmissions.
Table 4.16 RETIC Parameters -- Group 13 Field Name Retic Run Date Retic Run Time Retic ID Retic Cassette Position RET% RET# MRV Test Type CDR, CR, R CDR, CR, R CDR, CR, R CDR, CR, R CDR, CR, R, Edited CDR, CR, R, Edited CDR, CR, R, Edited 1G.1 + Retics X X X X X X X
P/N 4277303B
4-21
Table 4.16 RETIC Parameters -- Group 13 (Continued) Field Name MI (now called IRF) Test Type CDR, CR, R, Edited 1G.1 + Retics X
Retic Run Date Field: Tag

R E T D A T E
Data Format
N N / N N / N N
Data Length
9 bytes
Comments
Retic Run Date. Output is month/day/year, regardless of the system time configuration. Note: In the case of a CBC/Retic or CBC/Diff/Retic sample run, the Date and Time of the General Information group are duplicated in the RETDATE and RETTIME fields.
Retic Run Time Field: Tag

R E T T I M E
Data Format
N N : N N : N N
Data Length
9 bytes
Comments
Retic Run Time. Output is on 24 hours, regardless of the system time configuration. Time output is hours:minutes:seconds. Note: In the case of a CBC/Retic or CBC/Diff/Retic sample run, the Date and Time of the General Information group are duplicated in the RETDATE and RETTIME fields.
Retic ID Field: Tag

R I D
Data Format
X X X X X
Data Length
17 bytes
Comments
See Note**
**Note: In the case of a CBC/Retic or CBC/DIFF/Retic sample run, the Sample ID transmitted in the ID1 field is duplicated in this field.
4-22
P/N 4277303B
Retic Cassette/Position Field: Tag Data Format

X X X X / X X
Data Length
8 bytes
Comments
This field only applies to the 1G.1+ Retics host transmission protocol.
R C A S S / P O S
Retic Parameter Results Field: Tag

A A A A
Data Format
X X X X X X A A A
Data Length
Comments
See Below.**
6 char numeric 3 char flag data max. data max.
Result Format:** The parameter result will be transmitted in the Reporting Units with the extra digit criteria selected on the LH 750 Workstation. Numeric Data: ** If the numeric data of each format (for example, xx.xx) does not contain a decimal number, then it contains one of the following:
Result Comments Incomplete computation Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2) Invalid data: could not calculate RET#. Retic parameter result overrange RET# > 999.9 x 106cells/L RET% > 100%
..... :::::
????? +++++
Flag Data: ** The flag data characters for the Retic Parameter Group fields will be as follows in Table 4.17:
Table 4.17 Flag Data for Retic Parameter Group Fields LH 750 Workstation Comments Review parameter Exceeds reference interval high limit Exceeds reference interval low limit Exceeds action high limit STKS 1G.1 + Retics R H L H
P/N 4277303B
4-23
Table 4.17 Flag Data for Retic Parameter Group Fields (Continued) Exceeds action low limit Exceeds critical high limit Exceeds critical low limit Operator edited result Recalculated result from edited result Delta check failed Partial aspiration/blood detector disabled Result exceeds linearity R L H L E e
If a Retic parameter is disabled, results for that parameter will NOT be transmitted. The IRF parameter result is transmitted with the label MI for backward compatibility reasons. DF5 LS DataPlot Group Fields 1G.1 + Retics format does not support transmission of this group. This group will be transmitted with field count of 00. DF6 OP DataPlot Group Fields 1G.1 + Retics format does not support transmission of this group. This group will be transmitted with field count of 00. RETIC Histogram Group 1G.1 + Retics format does not support transmission of this group. This group will be transmitted with field count of 00.
4-24
P/N 4277303B
LH 750 WORKSTATION TO HOST COMMUNICATIONS LH 750 WORKSTATION FORMAT
4.3
LH 750 WORKSTATION FORMAT

Message Structure
The Presentation deals with the high level format of the message. The data bytes of the transmission blocks, when collected together, exhibit the following high level format.
PREAMBLE TRANSMISSION IDENTIFICATION TEST a IDENTIFICATION GROUP x1 GROUP xn
TESTz IDENTIFICATION
GROUP x1
GROUP xn
POSTAMBLE
Preamble: The preamble marks the beginning of a message.

C L R F C L C L C L C L C L R F R F R F R F R F C L R F
Transmission Identification:
S TRANSMISSION C L TRANSMISSION C L TEST R F TEST R F COUNT 1 TRANSMISSION C L TEST R F N
OOO
The ASCII character "S" marks the beginning of the transmission. TRANSMISSION TEST COUNT defines the number of tests that will be transmitted in this particular transmission. Not all Test Types are sent in all transmissions. Each Transmission Test is identified by the order in which it appears after the preamble. The transmission test will never be transmitted out of order. The Transmission Tests are up to 32 characters long. Table 4.18 lists the available Transmission Tests and Test IDs by Test Type.
Table 4.18 Transmission Tests and Test IDs by Test Type Test Type C CD R CR CDR C & CD Control R Control CR Control CDR Control CD Latex CDR Latex Retic Latex Transmission Tests CBC CBC, DIFF RETIC CBC, RETIC CBC, DIFF, RETIC 5CC RETICC RETICC RETICC DLATEX LATEXC RLATEX Test IDs CBC CBC, DIFF RETIC CBC, RETIC CBC, DIFF, RETIC CBC, DIFF RETIC CBC, RETIC CBC, DIFF, RETIC CBC, DIFF CBC, DIFF, RETIC RETIC
Currently only manually entered data is transmitted.
P/N 4277303B
4-25
Test Identification:
T TEST ID C L R F GROUP COUNT C L R F
The ASCII character "T" marks the beginning of a test identification. TEST ID, see Table 4.18. GROUP COUNT defines the number of groups of data in this particular TEST Identification. Group:
G GROUP NUMBER C L R F FIELD COUNT C L R F FIELD 1 C L R F FIELD N C L R F
The ASCII character "G" marks the beginning of a group. A group follows the test identification fields. GROUP NUMBER defines the group number. See Group Definition. FIELD COUNT defines the number of fields in a particular group. See Field Count. FIELD defines a field within a group. See Fields. Note: When there is no data for a Group, the Group will not be sent. Test Type Count: The transmission identification section has a Test Type Count that identifies the number of tests contained within the transmission. Valid test type count can be from 0-255. The test type count uses two characters to give the ASCII representation of the hexadecimal value.
Group Count: Each test identification has a Group Count that identifies the number of groups contained within the test. Valid group counts can be from 0-255. The group count uses two characters to give the ASCII representation of the hexadecimal value.
Group Number: Each group identifier has a Group Number that identifies the group number contained within the group identifier. Valid group number can be from 0-255. The group number uses two characters to give the ASCII representation of the hexadecimal value.
4-26
P/N 4277303B
Field Count: Each Group has a Field Count that identifies the number of fields contained within the Group. Valid field counts can be from 0-255. The field count uses two characters to give the ASCII representation of the hexadecimal value.
Fields: Each Group is made up of variable length fields which may or may not be padded with SP (20H), or HT (09H) characters. A field may have a variable length tag preceding the data, separated by one or more SP (20H), or HT (09H) characters. Please note that all field transmissions will include a SP between the tag and data format (this will be implied in the following sections), as illustrated below: Tag
T I M E
Sep
Data Format
N N : N N : N N
The data portion of a field will only contain ASCII characters in the range of 20H to 7EH inclusive. Heading 6.2, VALID HOST COMMUNICATIONS ASCII CODES identifies the valid ASCII characters. Fields are separated with a CR (0DH) and an LF (0AH) character. Note: A field's position within a Group must not be used to identify it. Fields within a Group may appear in any order or may be omitted. The CR/LF should be used to find fields and the Tag used to identify it. ALL FIELDS ARE OF MAXIMUM 32 CHARACTERS LONG UNLESS OTHERWISE NOTED. The following abbreviations are used to describe the fields: SEP - one or more SP (20H) or HT (09H) characters. The syntax for the Data Format of a field is: A - Alpha characters N - Numeric characters X - Printable characters H - Hexadecimal characters O - Other characters Postamble:
C L R F C L R F C L R F
(a..z and A..Z and space) or (61H..7AH and 41H..5AH and 20H) (0..9 and + - .) or (30H..39H and 2BH 2DH 2EH) (20H..7EH) (0..9 and A..F) (CR and LF) or (0DH and 0AH)
The postamble marks the end of the current message.
P/N 4277303B
4-27
There are 24 groups defined in the current implementation of the LH 750 Workstation. Future revisions of LH 750 Workstation may include additional groups. Any future additions will not disrupt the order of the groups as currently defined.
Group Definition
The abbreviations used to identify tests the group applies to are:
C D R CBC DIFF RETICS
Table 4-19 lists the groups transmitted under 2A, 3A, GENS, and LH 750 Workstation formats.
Table 4.19 Transmitted Groups Group Number
1 2 3 4 5 6 7 8 9 10 14 15 16 26 27 31 32 34 35 36 37 38 50 51
Group Name
General Information CBC Parameters DIFF Count Parameters DIFF Percent Parameters Comments Definitive Flags Suspect Flags Conditional Flags Other Flags Demographics RBC Histograms PLT Histograms RETIC Parameters WBC Threshold WBC Threshold Full Histogram DIFF Latex Parameters Retic Latex Parameters Control Information Control CBC Parameters Control DIFF Count Parameters Control DIFF Percent Parameters Control Retics Parameters DIFF Graphics Retic Graphics C C C C C C C C C C C C C C
Tests
D R
LH 750 Workstation
X, * X
D D D D D D D D R R R R R R
X X X, * X, * X, * X, * X, * X, * X X R X X X
D R D R
X X X X
D D R D R
X X X X X
X = Supported by the LH 750, Workstation format. * = Indicates that the group information will not be repeated with every test.
4-28
P/N 4277303B
Details of Group fields are defined in the following sections. General Information Group Fields Table 4.20 identifies the field names, test types, and applicable format of General Information transmissions.
Table 4.20 General Information -- Group 1 Field Name Run Date Run Time Sample Age Sample ID Pre Assigned Sample ID Cassette / Position Pre Assigned Cassette / Position Sample ID Status Cassette / Position Status Sample Status Instrument ID Operator ID Dilution Factor Test Type C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C LH 750 Workstation X X X X X X X X X X X X ~
X = Supported by the LH 750, Workstation format. ~ = New for the LH 750 Workstation format.
P/N 4277303B
4-29
Run Date Field: Tag

D A T E
Data Format
N N / N N / N N
Data Length
8 bytes
Comments
Run Date. Output is month/day/year, regardless of the system date configuration.
Run Time Field: Tag

T I M E
Data Format
N N : N N : N N
Data Length
8 bytes
Comments
Run Time. Output is on 24 hours, regardless of the system time configuration. Time output is hours:minutes:seconds.
Sample Age Field: Tag

S A
Data Format
N N N N : N N : N N
Data Length
11 bytes
Comments
Age of sample at run time. Format is DD HH:MM:SS. Computed from Draw Date and Draw Time. This field will not appear if draw date and time are invalid or after run date and time.
Sample ID Field: Tag

I D 1
Data Format
X X X X X X X X
Data Length
16 bytes
Comments
Bar code read on tube by instrument at aspiration time, entered on the Diluter keypad for Manual mode aspiration, or ID generated by Auto-numbering feature.
4-30
P/N 4277303B
Pre Assigned Sample ID Field: Tag

P I D 1
Data Format
X X X X X X
Data Length
16 bytes
Comments
PRE ASSIGNED SAMPLE ID

C A S S P O S
Data Format
A C C C C P P
Data Length
7 bytes
Comments
CASS/POS output is cassette number (C)/ cassette position (P). Data values for 'A' are: A for Automatic. M for Manual.
Pre-Assigned Cassette/Position Field: Tag

P C A S S P O S
Data Format
C C C C P P
Data Length
6 bytes
Comments
CASS/POS entered through ToDo list. Format is cassette number (C)/ cassette position (P). The sample mode is not specified at preassignment time.
Sample ID Status Field: Tag

I D 1 S T A T U S
Data Format
A
Data Length
1 byte
Comments
Data Values: P - positive ID E - edited ID N - Auto-numbered ID (20H) - not edited, not auto-numbered and not part of positive identification scheme. Precedence order is: E, N, P and (20H).
P/N 4277303B
4-31
Cassette/Position Status Field: Tag

C / P S T A T U S
Data Format
A
Data Length
1 byte
Comments
Data Values: P - positive ID E - edited ID (20H) - not edited and not part of positive identification scheme. Precedence order is: E, P and (20H).
Sample Status: Tag

S A S T A T U S
Data Format
X X X X X
Data Length
8 bytes
Comments
See Table 4.21.
Table 4.21 Possible Values for Sample Status Possible Value PART ASP NO READ NO MATCH MISMATCH INC COL COMPLETE LH 750 Workstation X X X X X X Comments Partial aspiration Bar code not read Match not found in ToDo list Non positive ID mismatch Incomplete collation No ToDo List errors
X in the LH 750 Workstation column = Supported by the LH 750 Workstation format.
Instrument ID: Tag

I N S T
Data Format
A A A A A
Data Length
16 bytes
Comments
Identifies the LH 750 instrument this sample was run on.
Operator ID: Tag

O P R
Data Format
A A A A A
Data Length
14 bytes
Comments
Identifies the user logged on the LH 750 Workstation at the time this sample run was analyzed.
4-32
P/N 4277303B
Dilution Factor: Tag Data Format

N N N N N
Data Length
5 bytes
Comments
Identifies amount by volume by which sample is diluted.This field is always transmitted. ll have the default value of 1.0 when used. It is only used in CBC Only transmissions. The parameters affected are WBC, RBC, PLT and HGB.
D F A C T
CBC Parameters Group Fields Table 4.22 identifies the field names, test types, and applicable format of CBC Parameter transmissions.
Table 4.22 CBC Parameters -- Group 2 Field Name WBC RBC HGB HCT MCV MCH MCHC RDW PLT PCT MPV PDW Test Type C, CD, CDR, CR, Edited C, CD, CDR, CR, R, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited LH 750 Workstation X X X X X X X X X X X X
X = Supported by the LH 750 Workstation format.
Each field in the CBC Parameters Group will have the following format: Tag
A A A A
Data Format
X X X X A A A A
Data Length
Comments
See Below.**
1 char separator min.
P/N 4277303B
4-33
Result Comments Incomplete computation Parameter overrange Parameter voteout
.....
+++++
----Flag Data: **
The flag data characters for the CBC Parameter Group Fields are as follows in Table 4.23:
Table 4.23 Flag Data for CBC Parameter Group Fields LH 750 Workstation Flags 4 (characters) R H L a a c c E e * D P + H L H L Review parameter Exceeds reference interval high limit Exceeds reference interval low limit Exceeds action high limit Exceeds action low limit Exceeds critical high limit Exceeds critical low limit Operator edited result Recalculated result from edited result MCV threshold failure Delta check failed Partial aspiration/blood detector disabled Result exceeds linearity
Comments
DIFF Count Parameters Group Fields Table 4.24 identifies the field names, test types, and applicable format of DIFF Count Parameter transmissions.
Table 4.24 DIFF Count Parameters -- Group 3 Field Name LY# MO# Test Type CD, CDR, Edited CD, CDR, Edited LH 750 Workstation X X
4-34
P/N 4277303B
Table 4.24 DIFF Count Parameters -- Group 3 (Continued) NE# EO# BA# NRBC# CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited X X X X
Each field in the DIFF Count Parameters Group will have the following format: Tag
A A A A
Data Format
X X X X A A A A
Data Length
Comments
See Below.**
Result Comments Incomplete computation Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2) Invalid data: could not calculate DIFF#. WBC parameter overrange or sum of DIFF# parameters is greater than 500.0 x 106 cells/L (whole blood) or sum of DIFF# parameters is greater than 600.0 x 106 cells/L (diluted).
..... :::::
????? +++++
Flag Data: ** The flag data characters for the DIFF Count Parameter Group fields are as follows in Table 4.25:
P/N 4277303B
4-35
Table 4.25 Flag Data for DIFF Count Parameter Group Fields LH 750 Workstation Flags (4 characters) R H L a a c c E e D P + H L H L
Comments Review parameter: WBC threshold failure or WBC exceeds linearity or sum of DIFF% results exceeds 100. Exceeds reference interval high limit Exceeds reference interval low limit Exceeds action high limit Exceeds action low limit Exceeds critical high limit Exceeds critical low limit Operator edited result Recalculated result from edited result Parameter value exceeds Delta check limits Partial aspiration/blood detector disabled Result exceeds linearity
DIFF Percent Parameters Group Fields Table 4.26 identifies the field names, test types, and applicable format of DIFF Percent Parameter transmissions.
Table 4.26 DIFF Percent Parameters -- Group 4 Field Name LY% MO% NE% EO% BA% NRBC% Test Type CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited GENS Workstation X X X X X X
Each field in the DIFF Percent Parameters Group will have the following format: Tag
A A A A
Data Format
X X X X A A A A
Data Length
Comments
See Below.**
4-36
P/N 4277303B
Result Comments Incomplete computation Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2) Sum of DIFF% parameter results is not equal to 100% +0.1, or DIFF# parameters is greater than 600.0 x 106 cells/L (predilute).
..... :::::
+++++
Flag Data: ** The flag data characters for the DIFF Percent Parameter Group fields will be as follows in Table 4.27:
Table 4.27 Flag Data for DIFF Percent Parameter Group Fields LH 750 Workstation Flags (4 characters) R H L a a c c E e D P + H L H L Review parameter Exceeds reference interval high limit Exceeds reference interval low limit Exceeds action high limit Exceeds action low limit Exceeds critical high limit Exceeds critical low limit Operator edited result Recalculated result from edited result Delta check failed Partial aspiration/blood detector disabled Result exceeds linearity
Comments
P/N 4277303B
4-37
Comment Group Fields Table 4.28 identifies the field names, test types, and applicable format of Comment Group Field transmissions.
Table 4.28 Comment -- Group 5 Field Name Comment Test Type C, CD, CDR, CR, R LH 750 Workstation X
Note: This group will be transmitted only once per sample transmission. The Comment Group can have a variable number of fields which consist of a variable number of bytes. Each Comment field is made up of an ASCII string identifying the appropriate flag. Tag
C O M M E N T
Data Format
X X X X X X
Data Length
254 bytes
Comments
STKS format is only 64 characters long.
Definitive Flags Group Fields Table 4.29 identifies the field names, test types, and applicable format of Definitive Flag transmissions.
Table 4.29 Definitive Flags -- Group 6 Field Name Definitive Flag Test Type C, CD, CDR, CR, R LH 750 Workstation X
Note: This group will always be transmitted and will only be sent once per sample transmission. Tag
D E F I N I T
Data Format
X X X X X X
Data Length
Up to 128 bytes
Comments
See Below.**
** The possible definitive flags generated on the LH 750 Workstation are:

LH 750 Workstation Messages Leukopenia Leukocytosis Neutropenia % Neutropenia # Neutrophilia % Neutrophilia # Lymphopenia % Lymphopenia # Lymphocytosis %
4-38
P/N 4277303B
LH 750 Workstation Messages (Continued) Lymphocytosis # Monocytosis % Monocytosis # Eosinophilia % Eosinophilia # Basophilia % Basophilia # Anemia Anisocytosis 1+ Anisocytosis 2+ Anisocytosis 3+ Anisocytosis Microcytosis 1+ Microcytosis 2+ Microcytosis 3+ Microcytosis Macrocytosis 1+ Macrocytosis 2+ Macrocytosis 3+ Macrocytosis Hypochromia 1+ Hypochromia 2+ Hypochromia 3+ Hypochromia Erythrocytosis Pancytopenia Reticulocytosis Thrombocytopenia Thrombocytosis Small Platelets Large Platelets H & H Check Failed
Suspect Flags Group Fields Table 4.30 identifies the field names, test types, and applicable format of Suspect Flag transmissions.
Table 4.30 Suspect Flags -- Group 7 Field Name Suspect Flag Test Type C, CD, CDR, CR, R LH 750 Workstation X
Note: This group will always be transmitted and will only be sent once per sample transmission.
P/N 4277303B
4-39
Tag
S U S P E C T
Data Format
X X X X X X
Data Length
Up to 128 bytes
Comments
See Below.**
** The possible Suspect flags generated on the LH 750 Workstation are:

LH 750 Workstation Messages Low Event # Verify Diff Ne Blasts Ly Blasts Mo Blasts Imm NE 1 Imm NE 2 Variant LY NRBC Dimorphic Reds Red Cell Agglutination Verify Retic Abnormal Retic Pattern Platelet Clumps Giant Platelets WBC Interference RBC Interference Inconsistent Diff CD WBC Exceeds Linearity
Conditional Flags Group Fields Table 4.31 identifies the field names, test types, and applicable format of Conditional Flag transmissions.
Table 4.31 Conditional Flags -- Group 8 Field Name Conditional Flag Test Type C, CD, CDR, CR, R LH 750 Workstation X
Note: This group will be transmitted with the first test only in each sample transmission. Tag
C O N D I T I O N
Data Format
X X X X X X
Data Length
Up to 128 bytes
Comments
See Below.**
4-40
P/N 4277303B
**The possible Conditional flags generated on the LH 750 Workstation are:

LH 750 Workstation Messages Edited Data Preliminary Report Additional Test Report Incomplete Collation SYSTEM ALARM Completed Report Blood Detector Disabled Sample validated Sample not validated AutoValidated
AutoValidation: The auto validation flags show the validation status of test requests for a sample on printouts and within the results transmission. A functional example of the validation codes is described below. A specimen is run in CDR mode with a CDR report format requested. Suppose results are acceptable for CBC and Retic, but not for the Diff (e.g., a Decision Rule fails, such as If Blast [suspect flag], Perform Manual Diff). Note: The configuration of the Workstation affects the validation messages and codes. The CBC and Retic test groups are AUTOVALIDATED, but the Diff group is NOT VALIDATED. There are two ways to indicate this. A conditional text message SAMPLE NOT VALIDATED would be transmitted. Additionally, validation codes C AV, R AV, D NV indicate the status of the individual test groups. The Diff can later be validated manually, resulting in SAMPLE VALIDATED, C AV, R AV, D V. It is possible through use of the report format to have validation messages based on the report profile (report format). For example, using the scenario above, if a CBC report profile was requested, validation would be based solely on the results of the CBC group. For a complete explanation, refer to the LH 750 Workstation on-line help.
P/N 4277303B
4-41
Validation Flags AV NV V AutoValidated Not Validated Validated (manually)
Validation Codes C CBC parameter block D Diff parameter block H H&H parameter block R Retic parameter block W WBC/Hgb/Plt parameter block
One of the conditional text messages below will always be transmitted. r r r Sample not validated Sample validated AutoValidated
If Enable Validation Codes in Sytem Setup - [Communications] is checked (as described in Heading 3.16, ENABLE VALIDATION CODES), the individual test group validation status will be transmitted based on the profile selected and test mode run. The following columns display the possible codes transmitted for the individual groups:
Sample Not Validated C NV D NV R NV H NV W NV Sample Validated CV DV RV HV WV AutoValidated C AV D AV R AV H AV W AV
Other Flags Group Fields Table 4.32 identifies the field names, test types, and applicable format of Other Flag transmissions.
Table 4.32 Other Flags -- Group 9 Field Name Other Flag Test Type C, CD, CDR, CR, R LH 750 Workstation X
Note: This group will be transmitted only once in each sample transmission. Tag
O T H E R
Data Format
X X X X X X
Data Length
Up to 128 bytes
Comments
See Below.**
4-42
P/N 4277303B
**The other possible flags generated on the LH 750 Workstation are:

LH 750 Workstation Messages HLR% IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES HLR# IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES PCT IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES PDW IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES MSCV IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES
Note: These flags will be produced only for the research parameters that are transmitted. Demographics Table 4.33 identifies the field names, test types, and applicable format of Demographics Group Field transmissions.
Table 4.33 Demographics -- Group 10 Field Name Date of Birth User Field #1 User Field #2 User Field #3 Sex Location Physician Sample Drawn Date Sample Drawn Time Patient Identification Patient Last Name Patient First Name Sequence Number Profile Number of slides made Number of slides requested Report Profile Test Type C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R LH 750 Workstation X X X X X X X X X X X X X X X X X
Note: This group will be transmitted only once per sample transmission.
P/N 4277303B
4-43
Date of Birth Field: Tag

B I R T H
Data Format
N N / N N / N N N N
Data Length
10 bytes
Comments
Date of Birth Output is month/day/year, regardless of the system date configuration. The Date Of Birth Year is Always 4 Characters.
User Field #1: Tag

U F 1
Data Format
X X X X X X
Data Length
16 bytes
Comments
User Field #2: Tag

U F 2
Data Format
X X X X X X
Data Length
16 bytes
Comments
User Field #3: Tag

U F 3
Data Format
X X X X X X
Data Length
16 bytes
Comments
Sex Field: Tag

S E X
Data Format
A
Data Length
1 byte
Comments
Data Value: M Male F Female U Unknown
Location Field: Tag

L O C A T I O N
Data Format
X X X X X X
Data Length
16 bytes
Comments
4-44
P/N 4277303B
Physician Field: Tag

P H Y S I C I A N
Data Format
X X X X X X
Data Length
Up to 22 bytes
Comments
Concatenation of Physicians last and first name separated by a comma and a space. Example: Smith, Peter.
Sample Drawn Date Field: Tag

D D
Data Format
N N / N N / N N
Data Length
8 bytes
Comments
Sample Drawn Date. Output is month/day/year, regardless of the system date configuration.
Sample Drawn Time Field: Tag

D T
Data Format
N N : N N : N N
Data Length
8 bytes
Comments
Sample Drawn Time. Output is on 24 hours, regardless of the system time configuration. Time output is hours:minutes :seconds.
Patient Identification: Tag

P I D
Data Format
X X X X X X
Data Length
16 bytes
Comments
Patient Last Name: Tag

P L N
Data Format
X X X X X X
Data Length
16 bytes
Comments
P/N 4277303B
4-45
Patient First Name: Tag

P F N
Data Format
X X X X X X
Data Length
16 bytes
Comments
Sequence Number: Tag

S E Q U E N C E
Data Format
X X X X X X
Data Length
6 bytes
Comments
Number of Slides Made: Tag

S M
Data Format
N
Data Length
1 byte
Comments
Indicates how many slides were made for that sample on the LH 750 SlideMaker. Possible values are: 0 or 1. If 0, this field is not sent.
Number of Slides Requested: Tag

S R
Data Format
N
Data Length
1 byte
Comments
Indicates how many slides were requested through preassignment in the ToDo list for that sample. Possible values are: 0 or 1.
Report Profile Field: Tag

R P
Data Format
N N
Data Length
2 bytes
Comments
Report Profile possible values are from 01" to "10". Always transmitted.
Profile Selection: Data transmission reflects profile selection. Parameters not in the selected profile will not be transmitted. Test blocks (e.g., CBC, DIFF , etc.) which are not in the current profile will not be transmitted. If a complete test block is omitted from the current profile, the General Information Block (for sample identification) will still be sent.
4-46
P/N 4277303B
RBC Histogram Group Fields Table 4.34 identifies the field names, test types, and applicable format of RBC Histogram Group Field transmissions.
Table 4.34 RBC Histogram -- Group 14 Field Name RBC Histogram Test Type C, CD, CDR, CR LH 750 Workstation X
If CBC histograms graphic transmission is disabled, this group will NOT be transmitted. RBC Histogram Field: Tag
R B C H
Data Format
H H H H H H
Data Length
512 bytes
Comments
See Chapter 8 for graphic construction.
PLT Histogram Group Fields Table 4.35 identifies the field names, test types, and applicable format of PLT Histogram Group Field transmissions.
Table 4.35 PLT Histogram -- Group 15 Field Name PLT Histogram PLT Fit Histogram Test Type C, CD, CDR, CR C, CD, CDR, CR LH 750 Workstation X X
If CBC histograms graphic transmission is disabled, this group will NOT be transmitted. PLT Histogram Field: Tag
P L T H
Data Format
H H H H H H
Data Length
256 bytes
Comments
Note: When the PLT histogram graphic transmission is enabled the data in the PLTF field is always sent. Ignore the data sent in the PLTF field if an R flag is present for the PLT parameter sent in the CBC Parameter Group. PLT Fit Histogram Field: Tag
P L T F
Data Format
H H H H H H
Data Length
512 bytes
Comments
P/N 4277303B
4-47
RETIC Parameters Group Fields Table 4.36 identifies the field names, test types, and applicable format of RETIC Parameters Group Field transmissions.
Table 4.36 RETIC Parameters -- Group 16 Field Name RET% RET# MRV MSCV IRF HLR% HLR# Test Type CDR, CR, R, Edited CDR, CR, R, Edited CDR, CR, R, Edited CDR, CR, R, Edited CDR, CR, R, Edited CDR, CR, R, Edited CDR, CR, R, Edited LH 750 Workstation X X X X X X X
Tag
A A A A
Data Format
X X X X A A A A
Data Length
Comments
See Below.**
Result Comments Incomplete computation Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2) Invalid data: could not calculate RET#. Retic parameter result overrange RET# > 999.9 x 106 cells/L RET% > 100%
..... :::::
????? +++++
Flag Data: ** The flag data characters for the Retic Parameter Group fields will be as follows in Table 4.37: 4-48
P/N 4277303B
Table 4.37 Flag Data for Retic Parameter Group Fields LH 750 Workstation Flags (4 characters) R H L a a c c E e D P + H L H L Review parameter Exceeds reference interval high limit Exceeds reference interval low limit Exceeds action high limit Exceeds action low limit Exceeds critical high limit Exceeds critical low limit Operator edited result Recalculated result from edited result Delta check failed Partial aspiration/blood detector disabled Result exceeds linearity
Comments
MRV, MSCV, HLR%, HLR# and IRF results will be transmitted only if they have been enabled. If a Retic parameter is disabled, results for that parameter will NOT be transmitted. The LH 750 Workstation does not support transmission of MI and OTHER fields. These fields will NOT be transmitted. WBC Threshold Group Fields Table 4.38 identifies the field names, test types, and applicable format of the WBC Threshold Monitor Group Field transmissions.
Table 4.38 WBC Threshold Monitor Group 26 Field Name WBC Threshold Monitor Test Type C, CD, CDR, CR LH 750S Workstation X
If CBC histograms graphic transmission is disabled, this group will NOT be transmitted. WBC Threshold Monitor Field: Tag
W B C T
Data Format
H H H H H H
Data Length
112 bytes
Comments
WBC Threshold Full Histogram Group Fields This group has the same description as WBC Threshold Group Fields with the exception that additional data elements are added.
P/N 4277303B
4-49
Table 4.38.1 WBC Full Histogram Monitor Group 27

Field Name WBC Threshold Monitor Test Type C, CD, CDR, CR LH 750S Workstation X
WBC Threshold Full Monitor Field: Tag

W B C F
Data Format
H H H H H H
Data Length
512 bytes
Comments
DIFF Latex Parameters Group Fields Table 4.39 identifies the field names, test types, and applicable format of DIFF Latex Parameters Group transmissions.
Table 4.39 DIFF Latex Parameters -- Group 31 Field Name PRIMER V_MN C_MN S_MN V_CV C_CV S_CV Test Type F55, F57 F55, F57 F55, F57 F55, F57 F55, F57 F55, F57 F55, F57 LH 750 Workstation X X X X X X X
Each field in the DIFF Latex Parameters Group will have the following format: Tag
A A A A
Data Format
X X X X A
Data Length
Comments
See Below.**
Result Format: ** The parameter result will be transmitted in the Reporting Units with the extra digit criteria selected on the LH 750 Workstation.
4-50
P/N 4277303B
Numeric Data: ** If the numeric data of each format (for example, xx.xx) does not contain a decimal number, then it contains one of the following:
Result Comments Incomplete computation Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
..... :::::
Flag Data: **
The flag data characters for the DIFF Latex Parameter Group fields will be as follows as in Table 4.40:
Table 4.40 Flag Data for DIFF Latex Parameters Group Fields LH 750 Workstation Flags (1 character) H L
Comments Greater than expected value range. Less than expected value range.
RETIC Latex Parameters Group Fields Table 4.41 identifies the field names, test types, and applicable format of RETIC Latex Parameters Group transmissions.
Table 4.41 RETIC Latex Parameters -- Group 32 LH 750 Workstation X X X X X X X
Field Name PRIMER V_MN C_MN S_MN V_CV C_CV S_CV
Test Type F56, F57 F56, F57 F56, F57 F56, F57 F56, F57 F56, F57, F56, F57
P/N 4277303B
4-51
Each field in the RETIC Latex Parameters Group will have the following format: Tag
A A A A
Data Format
X X X X A
Data Length
Comments
See Below.**
Result Format: ** The parameter result will be transmitted in the Reporting Units with the extra digit criteria selected on the LH 750 Workstation. Numeric Data:** If the numeric data of each format (for example, xx.xx) does not contain a decimal number, then it contains one of the following:
Result Comments Incomplete computation Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
..... :::::
Flag Data: **
The flag data characters for the RETIC Latex Parameter Group fields will be as follows as in Table 4.42:
Table 4.42 Flag Data for RETIC Latex Parameters Group Fields LH 750 Workstation Flags (1 character) H L
Comments Greater than expected value range. Less than expected value range.
Table 4.43 identifies the field names, test types, and applicable format of Control Information Group transmissions NOTE: Parameter selection does not affect control transmissions..
Table 4.43 Control Information -- Group 34 Field Name Run number Lot number User Control Name Expiration Date Test Type C, CD, CDR, CR, R, LATEX C, CD, CDR, CR, R, LATEX C, CD, CDR, CR, R, LATEX C, CD, CDR, CR, R, LATEX LH 750 Workstation * X X X
4-52
P/N 4277303B
Table 4.43 Control Information -- Group 34 (Continued) Run Date Run Time Operator IQAP ID Delete Flag Shift Cassette / Position Reference RBC Count C, CD, CDR, CR, R, LATEX C, CD, CDR, CR, R, LATEX C, CD, CDR, CR, R, LATEX C, CD, CDR, CR, R, LATEX C, CD, CDR, CR, R, LATEX C, CD, CDR, CR, R, LATEX C, CD, CDR, CR, R, LATEX R X X X X X X X X
X = Supported by the LH 750 Workstation format. * = Not supported by the LH 750 Workstation format.
Run Number Field: The LH 750 Workstation does not support transmission of this field. This field will NOT be transmitted. Lot Number Field: Tag
L O T #
Data Format
N N N N
Data Length
16 bytes
Comments
User Control Name Field: Tag

U C N A M E
Data Format
X X X X X X X
Data Length
12 bytes
Comments
Expiration Date Field: Tag

E X P D A T E
Data Format
N N / N N / N N
Data Length
8 bytes
Comments
Expiration Date. is month/day/year, regardless of the system date configuration.
P/N 4277303B
4-53
Run Date Field: Tag

D A T E
Data Format
N N / N N / N N
Data Length
8 bytes
Comments
Run Date. Output is month/day/year, regardless of the system date configuration.
Run Time Field: Tag

T I M E
Data Format
N N : N N : N N
Data Length
8 bytes
Comments
Run Time. Output is on 24 hours, regardless of the system time configuration. Time output is hours:minutes: seconds
Operator Field: Tag

O P E R A T O R
Data Format
X X X
Data Length
14 bytes
Comments
IQAP ID Field: Tag

I Q A P
Data Format
X X X X
Data Length
12 bytes
Comments
Delete Flag Field: Tag

D F L A G
Data Format
X
Data Length
1 byte
Comments
Run was excluded from statistical calculation on review screen. Y = Run excluded. SP = Run not excluded.
4-54
P/N 4277303B
Shift Field: Tag

S H I F T
Data Format
X
Data Length
1 byte
Comments
Possible values are 0, 1, 2, or 3. Reference the Online Help for more information.

C A S S P O S
Data Format
A C C C C P P
Data Length
7 bytes
Comments
CASS/POS output is cassette number (C) / cassette position (P). Data values for A are: A - Automatic sampling M - Manual sampling
Reference RBC Count Field: Tag

R R B C
Data Format
X X X X X
Data Length
5 characters numeric data max.
Comments
Reference WBC Count Field: Tag

R W B C
Data Format
X X X X X
Data Length
5 characters numeric data max.
Comments
Control CBC Parameters Group Fields Table 4.44 identifies the field names, test types, and applicable format of Control CBC Parameters Group transmissions NOTE: Parameter selection does not affect control transmissions..
Table 4.44 Control CBC Parameters -- Group 35 Field Name WBC RBC Test Type C, CD, CDR, CR C, CD, CDR, CR LH 750 Workstation X X
P/N 4277303B
4-55
Table 4.44 Control CBC Parameters -- Group 35 (Continued) HGB HCT MCV MCH MCHC RDW PLT PCT MPV PDW C, CD, CDR, CR C, CD, CDR, CR C, CD, CDR, CR C, CD, CDR, CR C, CD, CDR, CR C, CD, CDR, CR C, CD, CDR, CR C, CD, CDR, CR C, CD, CDR, CR C, CD, CDR, CR X X X X X X X X X X
Each field in the Control CBC Parameters Group will have the following format: Tag
A A A A
Data Format
X X X X A A A A 16 char numeric data max. 4 char flag data max.
Data Length
Comments
See Below.**
Result Comments Incomplete computation Total voteout Overrange
..... ----+++++
4-56
P/N 4277303B
Flag Data: ** The flag data characters for the Control CBC Parameter Group fields will be as follows as in Table 4.45:
Table 4.45 Flag Data for Control CBC Parameters Group Fields LH 750 Workstation Flags (4 characters) R H L * P + Review parameter. Exceeds expected range high limit Exceeds expected range low limit MCV threshold failure Partial aspiration/blood detector disabled Result exceeds linearity
Comments
Control DIFF Count Parameters Group Fields Table 4.46 identifies the field names, test types, and applicable format of Control DIFF Count Parameters Group transmissions. NOTE: Parameter selection does not affect control transmissions.
Table 4.46 Control DIFF Count Parameters -- Group 36 Field Name LY# MO# NE# EO# BA# NRBC# Test Type CD, CDR CD, CDR CD, CDR CD, CDR CD, CDR CD, CDR
LH 750 Workstation X X X X X
Each field in the Control DIFF count Parameters Group will have the following format: Tag
A A A A
Data Format
X X X X A A A A
Data Length
Comments
See Below.**
P/N 4277303B
4-57
Result Format: ** The parameter result will be transmitted in the Reporting Units with the extra digit criteria selected on the LH 750Workstation. Numeric Data:** If the numeric data of each format (for example, xx.xx) does not contain a decimal number, then it contains one of the following:
Result Comments Incomplete computation Invalid data: could not calculate DIFF#. Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
.....
?????
:::::
Flag Data: **
The flag data characters for the DIFF Count Parameter Group fields will be as follows in Table 4.47:
Table 4.47 Flag Data for DIFF Count Parameter Group Fields LH 750 Workstation Flags (4 characters) R H L P + Review parameter. Exceeds expected range high limit Exceeds expected range low limit Partial aspiration/blood detector disabled Result exceeds linearity
Comments
Control DIFF Percent Parameters Group Fields Table 4.48 identifies the field names, test types, and applicable format of Control DIFF Percent Parameters Group transmissions. NOTE: Parameter selection does not affect control transmissions.
Table 4.48 Control DIFF Percent Parameters -- Group 37 Field Name LY% MO% NE% EO% BA% NRBC% Test Type CD, CDR CD, CDR CD, CDR CD, CDR CD, CDR CD, CDR
LH 750 Workstation X X X X X
4-58
P/N 4277303B
Each field in the Control DIFF Percent Parameters Group will have the following format: Tag
A A A A
Data Format
X X X X A A A A
Data Length
Comments
See Below.**
Result Comments Incomplete computation Sum of DIFF% parameter results not equal to 100% +0.1 Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
.....
+++++
:::::
Flag Data: ** The flag data characters for the DIFF percent Parameter Group fields will be as follows in Table 4.49:
Table 4.49 Flag Data for Diff Percent Parameter Group Fields LH 750 Workstation Flags (4 characters) R H L P
Comments Review parameter. Exceeds expected range high limit Exceeds expected range low limit Partial aspiration/blood detector disabled
Control RETIC Parameters Group Fields Table 4.50 identifies the field names, test types, and applicable format of Control RETIC Parameters Group transmissions.
P/N 4277303B
4-59
NOTE: Parameter selection does not affect control transmissions.

Table 4.50 Control RETIC Parameters -- Group 38 Field Name RET% RET# MRV IRF MI OTHER Test Type CDR, CR, R CDR, CR, R CDR, CR, R CDR, CR, R CDR, CR, R CDR, CR, R LH 750 Workstation X X X X * *
X = Supported by the LH 750 Workstation format. * = Not supported by the LH 750 Workstation format.
Each field in the Control RETICS Parameters Group will have the following format: Tag
A A A A
Data Format
X X X X A A A A
Data Length
Comments
See Below.**
Result Comments Incomplete computation Invalid data: could not calculate RET#. Retic parameter result overrange RET# >999.9 x 106cells/L RET% >100% Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
.....
????? +++++
:::::
Flag Data: **
The flag data characters for the Control RETIC Parameter Group fields will be as follows in Table 4.51:
4-60
P/N 4277303B
Table 4.51 Flag Data for Control RETIC Parameter Group Fields LH 750 Workstation Flags (4 characters) R H L P Review parameter. Exceeds expected range high limit Exceeds expected range low limit Partial aspiration/blood detector disabled
Comments
DIFF Graphics Table 4.52 identifies the field names, test types, and applicable format of DIFF Graphics Group Field transmissions.
Table 4.52 DIFF DataPlot -- Group 50 Field Name DIFF graphics Test Type CD, CDR LH 750 Workstation X
If DIFF graphic transmission is disabled, this group will NOT be transmitted. DIFF Graphics Field: Tag
D I F F
Data Format
H H H H H H
Data Length
Variable
Comments
.
Retic Graphics Table 4.53 identifies the field names, test types, and applicable format of Retic Graphics Group Field transmissions.
Table 4.53 Retic DataPlot -- Group 51 Field Name Retic graphics Test Type CDR, CR, R LH 750 Workstation X
If Retic graphic transmission is disabled, this group will NOT be transmitted. Retic Graphics Field: Tag
R E T G
Data Format
H H H H H H
Data Length
Variable
Comments
P/N 4277303B
4-61
LH 750 WORKSTATION TO HOST COMMUNICATIONS HOST PARSING OF TRANSMITTED DATA
4.4
HOST PARSING OF TRANSMITTED DATA

Coulter reserves the right to make additions, deletions, and/or change the order of parameters in future releases. However, if the communications protocol is adhered to as specified in this document, future releases will not require reprogramming on the part of the host. We do not guarantee the appearance of any particular parameter. Neither do we guarantee any particular order to the transmitted data. This ensures that any program written to parse an existing transmission format will not have to be restructured if it is designed properly. Note that each information item is an ASCII character string. Because of delimiters, field terminators and so on, these information strings may be extracted from the transmitted data block without having to identify the strings at that time.
4-62
P/N 4277303B
5HOST TO LH 750 WORKSTATION COMMUNICATIONS (HOST ToDo LIST) 5

5.1 DATA-LINK
Protocol
The LH 750 Workstation requires full handshaking to receive data. The protocol is similar to that used for LH 750 Workstation to Host transmissions with the SYN character replaced with the ENQ character. The data is transmitted as a sequence of up to 255 blocks of data of 256 [or 128] bytes each. Generally, these blocks contain 256 data bytes each, but due to the unique needs of differing hosts, the system is configurable to allow shorter blocks with 128 data bytes. The final block must be padded with ASCII spaces (20H) if it has less than the number of data bytes necessary to complete. .
Host Computer (SENDER) LH 750 Workstation (RECEIVER) SYN indicates receiver busy, prepare to receive sample(s) record.
ENQ ENQ/SYN (NAK indicates receiver abort} X X ACK/NAK Exactly 256 or 128 data bytes 4-byte CRC ETX 2-byte data block STX 2-byte blk number
Send block and await response. Repeat data block and response until done. ACK/NAK/ENQ After each block ENQ All Done ACK/NAK/ENQ
DLE
Accept / Reject
PN 4277303B
5-1
HOST TO LH 750 WORKSTATION COMMUNICATIONS (HOST ToDo LIST) DATA-LINK
The LH 750 Workstation ACKs or NAKs each block sent by the Host. If the LH 750 Workstation NAKs a block, the Host must retransmit that block. If the Workstation NAKs the same block 3 times in a row, the Workstation aborts and is then prepared to receive the next transmission. If the LH 750 Workstation sends an ENQ after the reception of any block, the Host must retransmit all blocks starting with the first, but should not resend the initial ENQ character. Some common reasons why the LH 750 Workstation would NAK a block are: bad block sequence number, incorrect block byte count, CRC mismatch, etc. If the Data Link transmission successfully completes, the LH 750 Workstation then transmits a DLE character followed by a single ASCII character indicating whether the transmission is accepted or rejected at the presentation level. The ASCII characters following the DLE are listed below: The following demonstrates the above protocol:
Host Computer (SENDER) ENQ (ready) ENQ (go ahead) or SYN (busy) # of data blocks (2 bytes) ACK (read to receive) or NAK (receiver abort) send data blocks Data Block ACK (block received ok) or for each block NAK (retransmit this block) or ENQ (retransmit all blocks, starting with block #1) LH 750 Workstation (RECEIVER)
5-2
P/N 4277303B
HOST TO LH 750 WORKSTATION COMMUNICATIONS (HOST ToDo LIST) DATA-LINK
ENQ (all done) ACK (transmission accepted) or NAK (transmission rejected) (Presentation Level Response) DLE A (transmission accepted, ready for next) or B (transmission accepted, do NOT send more) or C (transmission rejected, retry) or D (transmission rejected, abandon) or *E (transmission rejected, at least one duplicate pending test request in sample request) F (transmission rejected, patient ID already exists in LH 750 database) *Please Note: User may select to replace DLE E with DLE C in the LIS section of the Communications Screen in the LH 750 Workstation System Setup. This option is provided for backward compatibility and is not the optimum configuration for the interface.
Return Codes For the Delete request, codes A, B, C, and D still apply. In addition to these, the following codes are returned for the Delete Function only: G Error in Patient ID field format H Error in Test field format I ToDo list Item not Deleted
P/N 4277303B
5-3
HOST TO LH 750 WORKSTATION COMMUNICATIONS (HOST ToDo LIST) PRESENTATION
Data Block Structure

Byte # 1 2 3 4 STX BLK NBR MS CHAR BLK NBR LS CHAR DATA BYTES 259 (131) 260 (132) 261 (133) 262 (134) 263 (135) 264 (136) CRC MSB MS CHAR CRC MSB LS CHAR CRC LSB MS CHAR CRC LSB LS CHAR ETX 1 byte 1 byte 1 byte 1 byte 1 byte # of bytes 1 byte 1 byte 1 byte 256 (128) bytes C R C
5.2
PRESENTATION
Message Structure
PREAMBLE INTRO FIELD 1
0 0 0
FIELD N
The data which is blocked for transmission will consist of a sequence of ASCII lines each terminated by a CR/LF pair. The block boundaries for the 256 [or 128] byte blocks would have no special significance with respect to this data. The data may be thought of as a large ASCII buffer which is being sent 256 [or 128] bytes at a time.
Preamble Field SOH 2-byte num fields 00 to FF CR LF
5-4
P/N 4277303B
Intro Field 2-byte record type For ToDo list WL AD UN RU DE 2-byte operation CR LF
Each Field ASCII Tag ASCII Data CR LF
Repeat until all fields given.
The 2-byte num fields indicate the number of fields that follow the preamble field, that is, the intro field plus all data fields. The record type indicates the target data set, for example, "WL" for ToDo list (TDL). The operation code indicates the action to be performed: The ASCII Tag is unique to each field within each record type. DLE Responses From The Workstation And Effects After A ToDo List Download INTRO FIELD: AD Add or Update 1. Conditions prior to ToDo List Download - No samples are in the ToDo list with the same Primary Identifier(s) and no samples are in the ToDo List or Completed list with the same Patient ID. a. b. 2. Workstation Response - DLE A or DLE B Effect - Add the downloaded request to the ToDo list.
Conditions prior to ToDo List Download - No sample(s) are in the ToDo list with the same Primary Identifier(s) but there are sample(s) are in the ToDo List and/or Completed list with the same Patient ID. a. b. Workstation Response - DLE A or DLE B Effect - Add the downloaded request to the ToDo list. Update the Last Name, First Name, Gender and Birth Date for all samples in the ToDo List and/or Completed list that have the same Patient ID.
3.
Conditions prior to ToDo List Download - Sample(s) are in the ToDo list with the same Primary Identifier(s) and sample(s) are in the ToDo List and/or Completed list with the same Patient ID. a. b. Workstation Response - DLE+E, or DLE+C if the workstation was configured to replace DLE+E with DLE+C Effect - Update the Last Name, First Name, Gender and Birth Date for all samples in the ToDo list and/or Completed List that have the same Patient ID.
P/N 4277303B
5-5
4.
Conditions prior to ToDo List Download - The downloaded ToDo list format is not correct. a. b. Workstation Response - DLE+C or DLE+D. Effect - No modifications are made to the ToDo list or to the Completed list.
INTRO FIELD: UN Unique 1. Conditions prior to ToDo List Download - No samples are in the ToDo list with the same Primary Identifier(s) and no samples are in the ToDo List or Completed list with the same Patient ID. If samples are deleted or edited to remove the patient ID then the Workstation will have to be rebooted before downloading a sample with the same patient ID using the Unique intro tag. Logging off and back on is not sufficient to allow the reuse of a patient ID. a. b. 2. Workstation Response - DLE A or DLE B Effect - Add the downloaded request to the ToDo list.
Conditions prior to ToDo List Download - No sample(s) are in the ToDo list with the same Primary Identifier(s) but there are sample(s) are in the ToDo List and/or Completed list with the same Patient ID. a. b. Workstation Response - DLE F Effect - No modifications are made to the ToDo list or to the Completed list.
3.
Conditions prior to ToDo List Download - Sample(s) are in the ToDo list with the same Primary Identifier(s) and sample(s) are in the ToDo List and/or Completed list with the same Patient ID. a. b. Workstation Response - DLE F Effect - No modifications are made to the ToDo list or to the Completed list.
4.
Conditions prior to ToDo List Download - The downloaded ToDo list format is not correct. a. b. Workstation Response - DLE+C or DLE+D. Effect - No modifications are made to the ToDo list or to the Completed list.
INTRO FIELD: RU Reuse 1. Conditions prior to ToDo List Download - No samples are in the ToDo list with the same Primary Identifier(s) and no samples are in the ToDo List or Completed list with the same Patient ID. a. b. 2. Workstation Response - DLE A or DLE B Effect - Add the downloaded request to the ToDo list.
Conditions prior to ToDo List Download - No sample(s) are in the ToDo list with the same Primary Identifier(s) but there are sample(s) in the ToDo List and/or Completed list with the same Patient ID. a. Workstation Response - DLE A or DLE B
P/N 4277303B
5-6
HOST TO LH 750 WORKSTATION COMMUNICATIONS (HOST ToDo LIST) MESSAGE DEFINITION
b. 3.
Effect - Add the downloaded request to the ToDo list. Remove the Last Name, First Name, Gender and Birth Date for all samples in the ToDo List and/or Completed list
Conditions prior to ToDo List Download - Sample(s) are in the ToDo list with the same Primary Identifier(s) and sample(s) are in the ToDo List and/or Completed list with the same Patient ID. a. b. Workstation Response - DLE+E, or DLE+C if the workstation was configured to replace DLE+E with DLE+C Effect - Remove the Last Name, First Name, Gender and Birth Date for all samples in the ToDo list and the Completed list that have the same Patient ID.
4.
Conditions prior to ToDo List Download -. The downloaded ToDo list format is not correct. a. b. Workstation Response - DLE+C or DLE+D. Effect - No modifications are made to the ToDo list or to the Completed list
INTRO FIELD: DE Delete DE in this field is a command by the host to delete one or more test entries in the LH 750 Workstation ToDo List. See Section 5.4, Delete Message, for a specification for this function.
5.3
MESSAGE DEFINITION
Table 5.1 lists the fields and the format to which they apply. The table also identifies fields that require data transmission in another field in order to be included in the test request. If data is not in the associated field, the LH 750 Workstation ignores the field. For example, if a Patient ID is not transmitted, the LH 750 Workstation ignores any data included in the Patient Last Name field. After the Table is the list of possible fields, their field width and the appropriate ASCII Tag to be used for transmission. A field will have a variable length TAG preceding the data separated by one or more SP (20H) or HT (09H) characters. A field tag must be transmitted with data. If a field tag is transmitted without data, the Workstation rejects the whole sample request. If an invalid or unrecognized tag is transmitted, the LH 750 Workstation will ignore the field and go to the next field provided that the field to be ignored has the correct field format (i.e. is terminated by a CRLF).
Table 5.1 Message Definition Table LH 750 Workstation X X X X X Field Requires Test Patient ID Patient ID Test Patient ID
Field Name Patient ID Patient Last Name Patient First Name Sequence Number Birth Date
P/N 4277303B
5-7
Table 5.1 Message Definition Table (Continued) Sex Location Physician Last Name Physician First Name User Field 1 User Field 2 User Field 3 Comments Report Profile Sample Drawn Date Sample Drawn Time Test Slide Request X X X X X X X X X X X X X Test
Patient ID Test Test Test Test Test Test Test Test Test Test
Only one samples demographics can be sent per transmission.
FIELDS CANNOT HAVE LEADING OR TRAILING SPACES (MESSAGE WILL BE REJECTED).
5-8
P/N 4277303B
Patient ID Field: Tag

I D
Data Format
X X X X X
Data Length
16 bytes
Comments
Patient ID is 16 characters, alphanumeric. Value in Intro field determines what is done: AD UN RU See Heading 5.2, PRESENTATION for details.
Patient Last Name Field: Tag

P L
Data Format
X X X X X
Data Length
16 bytes
Comments
Patient Last Name is 16 characters, alphanumeric. Requires Patient ID.
Patient First Name Field: Tag

P F
Data Format
X X X X X
Data Length
16 bytes
Comments
Patient First Name is 16 characters, alphanumeric. Requires Patient ID.
Sequence Number Field: Tag

S N
Data Format
N N N N N N
Data Length
6 bytes
Comments
Sequence Number
P/N 4277303B
5-9
Birth Date Field: Tag

B D
Data Format
N N / N N / N N N N
Data Length
10 bytes
Comments
Date format must be the same as the Workstation configuration. Any of the following formats can be used. DD/MM/YYYY, MM/DD/YYYY, or YYYY/MM/DD. Requires Patient ID.
Sex Field: Tag

S X
Data Format
A
Data Length
1 byte
Comments
Data Value: M - Male F - Female U - Unknown O - Other (equivalent to Unknown) Requires Patient ID.
Location Field: Tag

L N
Data Format
X X X X X
Data Length
16 bytes
Comments
Physician Last Name Field: Tag

D L
Data Format
X X X X X
Data Length
16 bytes
Comments
Physician Last Name is 16 characters, alphanumeric.
Physician First Name Field: Tag

D F
Data Format
X X X X X
Data Length
16 bytes
Comments
Physician First Name is 16 characters, alphanumeric.
5-10
P/N 4277303B
User Field 1 Field: Tag

U 1
Data Format
X X X X X
Data Length
16 bytes
Comments
Requires Test.

U 2
Data Format
X X X X X
Data Length
16 bytes
Comments
Requires Test.

U 3
Data Format
X X X X X
Data Length
16 bytes
Comments
Requires Test.
Comments Field: Tag

C M
Data Format
X X X X X
Data Length
64 bytes
Comments
Requires Test.
Report Profile Field: Tag

R P
Data Format
N N
Data Length
2 bytes
Comments
Report Profile possible values are from 01" to "10".
P/N 4277303B
5-11
Sample Drawn Date Field: Tag

E D
Data Format
N N / N N / N N
Data Length
8 bytes
Comments
Date format must be the same as the Workstation configuration. Any of the following formats can be used. DD/MM/YY, MM/DD/YY, or YY/MM/DD. Requires Test.
Sample Drawn Time Field: Tag

E T
Data Format
N N : N N
Data Length
5 bytes
Comments
Time output is on 24 hours, regardless of the system time configuration. Time output is hours:minutes. Requires Test.
Profile Selection: Data transmission reflects profile selection. Parameters not in the selected profile will not be transmitted. Test blocks (e.g., CBC, DIFF , etc.) which are not in the current profile will not be transmitted. If a complete test block is omitted from the current profile, the General Information Block (for sample identification) will still be sent.
5-12
P/N 4277303B
Test Field: Tag

T S
Data Format
X X X X X
Data Length
Up to 64 bytes
Comments
This field indicates test requests. Format for a test request is: TEST,TUBE ID,CASS/POS or TEST,TUBE ID, or TEST,CASS/POS . The commas are required as separators. Note that a comma is required after TUBE ID, even if it is not followed by a CASS/POS.See Table 5.2 for possible values. Required for transmission. The TS tag can repeat itself for as many tests as may be required. Only one test request can be sent per test tag occurrence. See Table 5.3 for sample and text request combinations.
Slide Request Field: Tag

S R
Data Format
N
Data Length
1 byte
Comments
Valid entries are 0 and 1. For a Retic Mode request, the only valid entry for the Slide Request field is 0. If a value > 1 is sent, this is converted by the LH 750 Workstation to a value of 1.
P/N 4277303B
5-13
Table 5.2 Possible Values and Examples of Test Requests Test Tag CBC LH 750 Workstation X Comment CBC only test. Example TS CBC,000101 TS CBC,123456789012, TS CBC,123456789012,000101 TS DIFF,000101 TS DIFF,123456789012, TS DIFF,123456789012,000101 TS CDR,000101 TS CDR,123456789012, TS CDR,123456789012,000101 TS RETIC,000101 TS RETIC,123456789012, TS RETIC,123456789012,000101 TS CR,000101 TS CR,123456789012, TS CR,123456789012,000101
DIFF
CBC/DIFF test
CDR
CBC/DIFF/Retic test
RETIC
Retic only test
CR
CBC/Retic test
X = Supported by the LH 750 Workstation format. *CASS POS is six characters TUBEID is no more than 16 characters
Table 5.3 Possible Combinations of Sample Requests with Multiple Test Requests Test CBC DIFF CDR RETIC * CR CBC NO NO NO YES NO DIFF NO NO NO YES NO CDR NO NO NO NO NO RETIC YES YES NO NO NO CR NO NO NO NO NO
* For a Retic Mode request, the only valid entry for the Slide Request field is 0.
5-14
P/N 4277303B
HOST TO LH 750 WORKSTATION COMMUNICATIONS (HOST ToDo LIST) Delete Message
A sample request with multiple test requests for CBC+DIFF or Retic only, would appear similar to the following:
. . . TS DIFF,123456,000101 TS RETIC,123,000203 . . .
5.4
Delete Message
When the Intro Field of a ToDo List upload from the host contains the Operation string DE (see page 5-77), the host has requested the deletion of one or more entries from the LH 750 Workstation ToDo List. The LH 750 Workstation allows deletion of preassigned test orders from the ToDo List by the host according to; Patient ID Sample ID Number Cassette Position Test Type The Sample ID, Cassette Position and Test Type are part of the Test Field. The formats for the Patient ID and Test fields are described in 5.3 Message Definitions. The Patient ID and Test field are the only fields of the message definition section used in the Deleted Function. All others are ignored and need not be sent. In the Deleted context, an ID or TS Tag followed by a CRLF will be interpreted as an empty Patient ID or Test Field. If the Patient ID field and the Test field are empty, the Workstation deletes all entries from the ToDo List. According to the above definition, these fields are empty when they have the following form:
Patient ID Field:
I T
D S
CR CR
LF LF
See Pages 5-5 & 5-9
Test Field:
See Pages 5-5 & 5-13
It is possible for a pre assignment to be made to the ToDo List that has no Patient ID. If the host requests deletion of this pre assignment, or some part of it, the transmission of the deletion request must contain no Patient ID field. Note that this is not the same as an empty Patient ID field. No field with an ID tag should be sent. See example 10. On page 5-83, the Data Format of the Test field is given as TEST,TUBE ID, CASS/POS or TEST,TUBE ID, or TEST,CASS/POS. The TUBE ID here is the SAMPLE ID. The commas
P/N 4277303B
5-15
are required as separators. Note that a comma is required after TUBE ID even if it is not followed by a CASS/POS. This Test Field Data Format has additional flexibility in the Delete Function only. NOTE: TEST,TUBE ID, represents an empty CASS/POS indicator and TEST,CASS/POS represents and empty TUBE ID indicator. Additional format flexibility in the Delete Function only is as follows. ,TUBE,ID,CASS/POSshall represent an empty TEST indicator. ,,CASS/POS shall represent empty TEST and TUBE ID indictors. ,TUBE ID, shall represent empty TEST and CASS/POS indicators. TEST,, shall represent empty TUBE ID and CASS/POS indicators. ,, shall represent empty TEST,TUBE ID, and CASS/POS indicators. NOTE: The last entry has the same effect as an empty Test field. In the Delete context, an Empty Indicator is interpreted to refer to all values of that indicator relative to the next highest indicator in the ToDo List. For example; If the TUBE ID indicator is not empty and the TEST indicator is empty, this is interpreted to mean delete all TESTs preassigned to this TUBE ID. An empty Test field refers to all preassigned tests relative to a given Patient ID (this is also true for the Test Field Data Format,,). It should be noted again, that if there is no Patient ID field, this is not the same as an empty Patient ID field. This is interpreted as referring to a preassignment with no Patient ID. The following table illustrates the variables.
5-16
P/N 4277303B
Table 5.4 Patient ID Deletions Example Patient ID Test ID Action
1 2 3 4 5 6 7 8 9 10 11
ID_<CR><LF> ID_123456 <CR><LF> ID_<CR><LF> ID_123456 <CR><LF> ID_123456 <CR><LF> ID_123456 <CR><LF> ID_123456 <CR><LF> ID_<CR><LF> ID_<CR><LF> * ID_<CR><LF>
TS_<CR>< LF> TS_<CR><LF> TS_CBC,,<CR><LF> TS_CBC,,<CR><LF> TS_CDR,987654,<CR>< LF> TS_CDR,,001001,<CR>< LF> TS_CBC,987654,001001, <CR>< LF> TS_CBC,001001 * TS_ , ,<CR><LF> TS_ ,ID12345,<CR><LF>
Delete all entries of all types in ToDo list. Delete all Patient ID 123456 entries of all test types in ToDo list. Delete all CBC tests from all Patient ID entries in ToDo list. Delete all CBC tests from Patient ID 123456. Delete CDR test from Tube ID 987654and all Cass/Pos for Patient ID 123456. Delete all Patient ID 123456 entries of CDR test type in ToDo list with Cass/Pos 001001. Delete all Patient ID 123456 entries of CBC test type in ToDo list with Tube ID 987654 and Cass/Pos 001001. Delete CBC test for all the Tube IDs with Cass/Pos 001001for all Patient IDs. Delete all entries of all types in ToDo list. Delete all entries in ToDo list without Patient IDs. Delete Tube ID from all test types.
* This field was not transmitted. NOTE: TS_<CR><LF> is to TS_, , <CR><LF>
P/N 4277303B
5-17
5-18
P/N 4277303B
6ASCII Tables 6
6.1 7-BIT ASCII CODES
) ) ) ) ) ) ) ) ) 0 0 0 0 0 1 0 1 0 0 1 1 1 0 0 1 0 1 1 1 0 1 1 1 + ) ) ) * ) ) + * + )
7 6 5 4 3 2 1 X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 0 0 0 0 1 1 1 1 0 0 0 0 1 1 1 1 0 0 1 1 0 0 1 1 0 0 1 1 0 0 1 1 0 1 0 1 0 1 0 1 0 1 0 1 0 1 0 1 NUL SOH STX ETX EOT ENQ ACK BEL BS HT LF VT FF CR SO SI DLE DC1 DC2 DC3 DC4 NAK SYN ETB CAN EM SUB ESC FS GS RS US SP ! # $ % & ( ) * + , . / 0 1 2 3 4 5 6 7 8 9 : ; < = > ? @ A B C D E F G H I J K L M N O P Q R S T U V W X Y Z [ \ ] ^ _ ` a b c d e f g h I j k l m n o p q r s t u v w x y z { | } ` DEL
6.2
VALID HOST COMMUNICATIONS ASCII CODES

) ) ) 4 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 ) ) ) 3 0 0 0 0 1 1 1 1 0 0 0 0 1 1 1 1 ) ) ) 2 0 0 1 1 0 0 1 1 0 0 1 1 0 0 1 1 ) ) ) 1 0 1 0 1 0 1 0 1 0 1 0 1 0 1 0 1 0 0 0 0 0 1 0 1 0 0 1 1 1 0 0 1 0 1 1 1 0 1 1 1
+ ) ) * ) + * + 7 6 5 X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X
SOH STX ETX ENQ ACK HT LF CR
DLE DC1
NAK SYN
SP ! # $ % & ( ) * + , . /
0 1 2 3 4 5 6 7 8 9 : ; < = > ?
@ A B C D E F G H I J K L M N O
P Q R S T U V W X Y Z [ \ ] ^ _
` a b c d e f g h I j k l m n o
p q r s t u v w x y z { | } ` DEL
Bold used only for Data-link control.
PN 4277303B
6-1
ASCII Tables VALID HOST COMMUNICATIONS ASCII CODES
6-2
P/N 4277303B
7CRC 7
7.1 CRC ALGORITHM
The CRC algorithm used is a modified CCITT CRC16 algorithm and is the same as used in the STKS analyzer. The polynomial for this algorithm is: X**16 + X**12 + X**5 + 1. Note: D = current data byte that is input to the algorithm. CRCLSB,CRCMSB = data byte. Least significant and most significant CRC accumulator bytes. x>>n means x is shifted n bits to right or is the same as x divided by 2n. x<<n means x is shifted n bits to left or is the same as x multiplied by 2n. //-----------------------------------------------------------------------------------------------------------------at beginning, CRCLSB = 0FFH (octal 377) (decimal 255) CRCMSB = 0FFH (octal 377) (decimal 255) then for each data byte in a block, X = D XOR CRCMSB X = X XOR ( X >> 4 ) CRCMSB = CRCLSB XOR ( X >> 3 ) XOR ( X << 4 ) CRCLSB = X XOR ( X << 5 ) and at end, CRCLSB = CRCLSB XOR 0FFH CRCMSB = CRCMSB XOR 0FFH //------------------------------------------------------------------------------------------------------------------
PN 4277303B
7-1
CRC CRC EXAMPLE WRITTEN IN ASM86
7.2
CRC EXAMPLE WRITTEN IN ASM86

The following is an example of CRC16 code written in assembler for 8086: ;------------------------------------------------------------------------------------------------------------------GET_CRC_BYTES PROC PUSH CX ;;This is the Algorithm used (CCITT CRC16): ; ; X = D XOR CRCMSB ; X = X XOR ( X >> 4 ) ; CRCMSB = CRCLSB XOR ( X >> 3 ) XOR ( X << 4 ) ; CRCLSB = X XOR ( X << 5 ) ; ;Data arrives in AL. ;Finished CRC is in BX ( BH = MSB, BL = LSB ) ;To use this algorithm: ; 1. Initialize BX to 0FFFFH. ; 2. At end: ; CRCLSB = CRCLSB XOR 0FFH. ; CRCMSB = CRCMSB XOR OFFH. ; XOR AL,BH ;X=D XOR CRCMSB (AL=X) <--- 1 MOV SHR XOR MOV MOV SHR XOR SHL XOR MOV MOV SHL XOR MOV AH,AL AL,4 AL,AH AH,AL CH,AL AL,3 AL,BL AH,4 AL,AH BH,AL AL,CH AL,5 AL,CH BL,AL ;Save X for later in AH ;Then AL=X >> 4 ;X=X XOR ( X >> 4 )<--- 2 ;Save X ;Save X ;Then AL= ( X >> 3 ) ;AL=CRCLSB XOR ( X >> 3 ) ;AH=( X << 4 ) ;CRCMSB=CRCLSB XOR ; ( X >> 3 ) XOR ( X << 4 )<--- 3 ;Recover X in AL ;AL=( X << 5 ) ;AL=X XOR ( X << 5 ) ;CRCLSB=X XOR ( X << 5 )<--- 4
POP CX RET GET_CRC_BYTES ENDP ;-------------------------------------------------------------------------------------------------------------------
7-2
P/N 4277303B
CRC CRC EXAMPLE WRITTEN IN C
7.3
CRC EXAMPLE WRITTEN IN C

The following is an example of CRC16 code written in C. //-----------------------------------------------------------------------------------------------------------------void calc_crc ( unsigned char DataByte ) { DataByte ^= crcmsb; DataByte ^= ( DataByte / 16 ); crcmsb = crclsb ^ ( DataByte / 8 ) ^ ( DataByte * 16 ); crclsb = DataByte ^ ( DataByte * 32 ); } //------------------------------------------------------------------------------------------------------------------
P/N 4277303B
7-3
CRC CRC EXAMPLE WRITTEN IN C
7-4
P/N 4277303B
8GRAPHIC RECONSTRUCTION 8
8.1 RBC, WBC THRESHOLD, AND PLT HISTOGRAMS
The histograms display a simple line graph which is representative of the count of events occurring in each A/D channel (or voltage level). The histograms are transmitted as 512 ASCII bytes for RBC (256 bytes for PLT, 112 bytes for WBC and 512 bytes for WBC Full Histogram) representing a 256-byte for RBC (128-byte for PLT, 56 bytes for WBC and 256 bytes for WBC Full Histogram) Hex array. It takes two ASCII bytes to represent one Hex byte. Each Hex byte represents a scaled count for each channel 0-255 (14-142 for PLT). The histograms can be constructed by plotting the count for each channel then drawing a line between plots. To display (or print) the histogram you must plot the channel vs. count. The channel represents the X-axis while the count is the Y-axis (see Figure 8.1). There are 128 channels for the platelet histogram, 256 channels for RBC, 56 channels for WBC and 256 channels for WBC Full Histogram. The value range is 14-255 (0D-FF hex) for the platelet histogram and 0-255 (00-FF hex) for the WBC and RBC histograms.
Figure 8.1 Plotting RBC, WBC Threshold and PLT Histograms
y C O U N T
<<
CHANNEL
8.2
LH 750 DATAPLOT FORMAT

The 2-dimensional dataplots communicate a greater amount of information to the viewer about the events. The following sections describe the format of the dataplot data and provide a how-to example of displaying the dataplot data on a raster device such as a video display or printer.
Dataplots
The dataplots consist of two major components: dataplot data and rendering information. Taken together, these two components allow a receiving application to display the dataplots in the same fashion as the LH 750 Workstation. The dataplot portion of the host transmission consists of a dataplot data header and one dataplot data block.
PN 4277303B
8-1
GRAPHIC RECONSTRUCTION LH 750 DATAPLOT FORMAT
Dataplot Data Header The dataplot data header tells the receiving application how large the dataplot transmission is and how many dataplot data blocks are contained in the transmission. A complete dataplot transmission, including the dataplot data header, is illustrated in Figure 8.2.
Figure 8.2 Dataplot Transmission
Dataplot data header Dataplot data block Dataplot Data Header Elements The dataplot data header contains two elements: the total length of the dataplot transmission and the number of dataplot data blocks in the transmission. The data are represented as one 16-bit integer which indicates the total size of the dataplot transmission and one 8-bit integer which indicates the total number of dataplot data blocks in the transmission (between 1 and 4). Only one block is supported currently, but up to four may be used in future software. See Figure 8.3 for details.
Figure 8.3 Dataplot Data Header
Transmission Length LSB Transmission Length MSB Total Number of Dataplot data blocks Dataplot Data Block The dataplot data block contains both the rendering information and the actual 2-dimensional dataplot data. Each dataplot data block is composed of four subsections: dataplot data block header, rendering information block, dataplot header and the dataplot data. Figure 8.4 illustrates the dataplot data block format.
Figure 8.4 Dataplot Data Block Format
Dataplot data block header Rendering information block Dataplot header Dataplot data
8-2
P/N 4277303B
Dataplot Data Block Header The dataplot data block header tells the receiving application about the type of dataplot being transmitted as well as details about any included rendering information. See Figure 8.5. The dataplot data block header has the following format:
Figure 8.5 Dataplot Data Block Header
Dataplot Type code
Dataplot Type Code is an 8-bit integer which represents the run type of the sample which produced this dataplot. It will be one of the following values: SC_TYPE_5DIFF (0x01) is 5 Part Differential Dataplot. SC_TYPE_RETIC (0x02) is Reticulocyte Dataplot. SC_TYPE_CD4 (0x04) is CD4 Subset Dataplot. SC_TYPE_CD8 (0x05) is CD8 Subset Dataplot. Dataplot Option Code is an 8-bit integer which contains option flags that pertain to the current dataplot. The following flags are currently defined and can be combined into Dataplot Option Code with a logical or when applicable: SO_COMPRESSED (0x01) indicates that the dataplot has been compressed. This option is not supported currently, but may be available in future software. SO_HAS_RENDER_INFO (0x02) indicates that a valid rendering information block follows. Dataplot width is a 16-bit integer which specifies the width of the dataplot. Dataplot height is a 16-bit integer which specifies the height of the dataplot.
This symbol indicates that at the time of this release this feature was not available.
Dataplot Option code
Dataplot width (LSB) Dataplot width (MSB) Dataplot height (LSB) Dataplot height (MSB)
Rendering Information Block The rendering information block contains information the receiver can use to reconstruct the dataplot as it was displayed by the LH 750 Workstation. The dataplots are color-coded to identify the population and dithered to code the density. The rendering information block contains color palettes and dither pattern bitmaps used by the LH 750 Workstation software when displaying or printing the dataplots. It is highly recommended that host software employ the same scheme when displaying the dataplots to maintain consistency of operator conclusions across platforms. See Figure 8.6. The structure of the rendering information block is as follows:
P/N 4277303B
8-3
Figure 8.6 Rendering Information Block
Rendering information block header Color palette Dithered display bitmap library Rendering Information Block Header The rendering information block header contains fields which indicate the size and format of the bitmaps and the color palette. It is sent on the first transmission after a user log-on and a shutdown/restart occurs. As notification is not provided when a shutdown/restart is initiated, the rendering block transmission cannot be predicted in advance. Figure 8.7 is an illustration of the rendering information block header.
Figure 8.7 Rendering Information Block Header Format
Rendering information block options
Rendering Information Block Options may be one or more of the following flags: RH_OPTIONS_NONE (0x00) indicates that no options are set.
Number of color palette table entries Number of bitmap library entries Bitmap width in pixels Bitmap height in pixels
It is an 8-bit integer. Number of Color Palette Table Entries is the number of entries in the color palette. It is an 8-bit integer. Number of Bitmap Library Entries is the number of bitmap patterns contained in the Dithered display bitmap library. It is an 8-bit integer. Bitmap Width in Pixels is the width of each bitmap in pixels. It is an 8-bit integer. Bitmap Height in Pixels is the height of each bitmap in pixels. It is an 8-bit integer.
Color Palette Each color palette entry contains three 8-bit integers which represent the red, green and blue values for that index as shown in Figure 8.8.
Figure 8.8 Palette Table Entry
Red Component Green Component Blue Component Dithered Display Bitmap Library The dithered display bitmap library contains the dithering bitmaps used by the LH 750 Workstation to display the dataplots. They indicate the density of a specific cell population. Receiving applications should use these bitmaps when generating displays to ensure that host
8-4
P/N 4277303B
displays will match LH 750 Workstation displays even when Coulter updates the dither patterns or population colors. The bitmap format is a standard raster scanned array of pixels. Each value is an 8-bit integer which corresponds to an entry in the color palette described above. Figure 8.9 describes the bitmap format.
Figure 8.9 Bitmap Format
0 Bitmap width in pixels
Bitmap height in pixels
7306002B
Each bitmap is stored as a column-major array of pixels represented as 8-bit integers. The array contains:
Bitmap width in pixels x Bitmap height in pixels 8-bit integers
Dataplot Header The dataplot header contains instructions of how the dataplot data is to be interpreted. The structure of the dataplot header is shown in Figure 8.10.
Figure 8.10 Dataplot Header
Dataplot data options
Dataplot data options may be one of the following flags: SO_OPTIONS_NONE (0x00) no option have been selected.
Dataplot datum size
Reserved Reserved
It is an 8-bit integer. Dataplot datum size is the size in bits of each point in the dataplot. It is usually 8 which represents a byte. It is an 8-bit integer. Reserved for future use and should be ignored. It is an 8-bit integer. Reserved for future use and should be ignored. It is an 8-bit integer.
P/N 4277303B
8-5
The format of the dataplot is shown in Figure 8.11.

Figure 8.11 Dataplot Data Format
0 Scatterplot width
Scatterplot height
7306003B
8-6
P/N 4277303B
TRADEMARKS
5C, AccuGate, the BECKMAN COULTER logo, BECKMAN COULTER, COULTER, COULTER CLENZ, COULTER COUNTER, GENS, GENS SM, GENS ST, LIN-C, LYSE S, ISOTON, S-CAL, ZAP-OGLOBIN and IntelliKinetics are trademarks of Beckman Coulter, Inc.
All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.
PN 4277303B
COULTER LH 750 SYSTEM HARD-COPY DOCUMENTATION
s s s
Getting Started PN 4277302 Host Transmission PN 4277303 Reference PN 4277248 Operators Guide PN 4277249
Overview of system hardware and software Specifications for transmitting to a host computer Use and Function Installation Operation Principles Specifications/Characteristics Precautions/Hazards References Glossary Index Available in hard copy by request. Controls and Indicators Startup QC Sample Analysis Data Analysis Shutdown Analyzer CRT Functions Workstation Run Samples Display To Do List Database Controls Setup Appendices Available in hard copy by request. Calibration Cleaning Procedures Replace/Adjust Procedures Troubleshooting Error Messages Available in hard copy by request. Combined index for Reference, Operators Guide, and Special Procedures and Troubleshooting manuals. Comes with hard copy of Operators Guide and Special Procedures and Troubleshooting. Use and Function Installation Operation Principles Specifications/Characteristics Operating Procedures Cleaning and Replacement Procedures Troubleshooting References Index Available in hard copy by request. Use and Function Installation Operation Principles Specifications/Characteristics Operating Procedures Cleaning and Replacement Procedures Troubleshooting References Index Available in hard copy by request.
Special Procedures and Troubleshooting PN 4277250 Master Index PN 4277301 SlideMaker Operators Guide PN 4277299 SlideStainer Operators Guide PN 4277300
The Getting Started booklet and the Host Transmission manual come with your LH 750 System. The information in the Reference manual, Operators Guide, Special Procedures and Troubleshooting manual, the SlideMaker Operators Guide and the SlideStainer Operators Guide comes from the Online Help System. Each of these hard-copy documents comes with a Master Index.
Come visit us at www.beckmancoulter.com
Copyright Beckman Coulter, Inc. 2001-2002 All Rights Reserved Printed on Recycled Paper
COULTER CORPORATION A Beckman Coulter Company Miami, Florida 33196-2500 USA

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COULTER LH 750 WORKSTATION

Host Transmission Specification

HARDWARE INTERFACE, 2-1 2.1 HARDWARE HANDSHAKE, 2-1

3.3 3.4 3.5 3.6 3.7 3.8 3.9

HARDWARE INTERFACE HARDWARE HANDSHAKE

Diagram of Hardware Handshake Mode

Low Level Protocol

High Level Protocol

DO NOT ASSUME FIXED OFFSETS

COMMUNICATION PARAMETERS TIME-OUT

3.5 3.6 3.7 3.8

COMMUNICATION PARAMETERS COMPATIBILITY

CLEARING THE SPOOLER

DISABLING HOST COMMUNICATIONS

ENABLE VALIDATION CODES

COMMUNICATION PARAMETERS Replace DLE E with DLE C

Replace DLE E with DLE C

Collate the ToDo List

4LH 750 WORKSTATION TO HOST COMMUNICATIONS 4

Full Software Handshake

Software Handshake Action

LH 750 WORKSTATION TO HOST COMMUNICATIONS DATA-LINK

Data Block Structure

# of bytes 1 byte 1 byte 1 byte 256 (128) bytes C R C

1 byte 1 byte 1 byte 1 byte 1 byte

LH 750 WORKSTATION TO HOST COMMUNICATIONS DATA-LINK

Group Definition Locator Table

LH 750 Workstation, Heading 4.3

LH 750 WORKSTATION TO HOST COMMUNICATIONS 1G.1 + RETICS FORMAT

1G.1 + RETICS FORMAT

Preamble The preamble marks the beginning of a message.

LH 750 WORKSTATION TO HOST COMMUNICATIONS 1G.1 + RETICS FORMAT

LH 750 WORKSTATION TO HOST COMMUNICATIONS 1G.1 + RETICS FORMAT

X = Supported by the LH 750, STKS 1G.1 + Retics format.

LH 750 WORKSTATION TO HOST COMMUNICATIONS 1G.1 + RETICS FORMAT

Run Date Field: Tag

Run Time Field: Tag

Cassette/Position Field: Tag

LH 750 WORKSTATION TO HOST COMMUNICATIONS 1G.1 + RETICS FORMAT

Sequence Number: Tag

1E Cassette/Position Field: Tag

ID #1 Status Field: Tag

Cassette/Position Status Field: Tag

ToDo List Status: Tag

*Data Values: GOOD = PART ASP = NO READ =

O.K. (Complete) Partial aspiration Bar code not read

LH 750 WORKSTATION TO HOST COMMUNICATIONS 1G.1 + RETICS FORMAT

6 char numeric data max.

3 char flag data max.

LH 750 WORKSTATION TO HOST COMMUNICATIONS 1G.1 + RETICS FORMAT

Comments Incomplete computation Parameter overrange Parameter voteout

..... +++++ -----

LH 750 WORKSTATION TO HOST COMMUNICATIONS 1G.1 + RETICS FORMAT

6 char numeric data max.

3 char flag data max.

LH 750 WORKSTATION TO HOST COMMUNICATIONS 1G.1 + RETICS FORMAT

6 char numeric data max.

3 char flag data max.

LH 750 WORKSTATION TO HOST COMMUNICATIONS 1G.1 + RETICS FORMAT

LH 750 WORKSTATION TO HOST COMMUNICATIONS 1G.1 + RETICS FORMAT

LH 750 WORKSTATION TO HOST COMMUNICATIONS 1G.1 + RETICS FORMAT

LH 750 WORKSTATION TO HOST COMMUNICATIONS 1G.1 + RETICS FORMAT

(N/A) Thrombocytopenia Thrombocytosis Small Platelets Large Platelets (N/A)