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Morphology
Colonies of Saccharomyces grow rapidly and mature in three days. They are flat, smooth, moist, glistening or dull, and cream to tannish cream in color. The inability to use nitrate and ability to ferment various carbohydrates are typical characteristics ofSaccharomyces.
Cellular morphology
Generally, they have a diameter of 2-8m and length of 3-25m. Blastoconidia (cell buds) are observed. They are unicellular, globose, and ellipsoid to elongate in shape. Multilateral (multipolar) budding is typical. Pseudohyphae, if present, are rudimentary. Hyphae are absent. Saccharomyces produces ascospores, especially when grown on V-8 medium, acetate ascospor agar, or Gorodkowa medium. These ascospores are globose and located in asci. Each ascus contains 1-4 ascospores. Asci do not rupture at maturity. Ascospores are stained with Kinyoun stain and ascospore stain. When stained with Gram stain, ascospores appear Gram-negative, while vegetative cells appear Gram-positive.
Candida is a genus of yeasts. Many species are harmless commensals or endosymbionts of hosts including humans, but other species, or harmless species in the wrong location, can cause disease. Candida albicans can cause infections (candidiasis or thrush) in humans and other animals, [3] [4] especially in immunocompromised patients. In winemaking, some species of Candida can create potential faults in wines. Grown in the laboratory, Candida appears as large, round, white or cream (albicans is from Latin meaning 'whitish') colonies with a yeasty odor on agar [14] plates at room temperature. C. albicans ferments glucose and maltose to acid and gas, sucrose to acid, and does not ferment lactose, which help [15] to distinguish it from other Candida species.
Rhizopus species grow as filamentous, branching hyphae that generally lack cross-walls (i.e., they are coenocytic). They reproduce by forming asexual and sexual spores. In asexual reproduction, sporangiospores are produced inside a spherical structure, the sporangium. Sporangia are supported by a large apophysate columella atop a long stalk, the sporangiophore. Sporangiophores arise among distinctive, root-like rhizoids. In sexual reproduction, a dark zygospore is produced at the point where two compatible mycelia fuse. Upon germination, a zygospore produces colonies that are genetically different from either parent.
Mucor is a microbial genus of approximately 3000 species of moulds commonly found in soil, digestive systems, plant surfaces, and rottenvegetable matter. Colonies of this fungal genus are typically coloured white to beige or grey and are fast-growing. Colonies on culture medium may grow to several centimetres in height. Older colonies become grey to brown in colour due to the development of spores. Mucor spores or sporangiospores can be simple or branched and form apical, globular sporangia that are supported and elevated by a columnshaped columella. Mucor species can be differentiated from molds of the genera Absidia, Rhizomucor, and Rhizopus by the shape and insertion of the columella, and the lack of rhizoids. Some Mucor species produce chlamydospores.
Penicillium is a genus of ascomycetous fungi of major importance in the natural environment as well as food and drug production. Members of the genus produce penicillin, a molecule that is used as an antibiotic, which kills or stops the growth of certain kinds of bacteria inside the body. According to the Dictionary of the Fungi (10th edition, 2008), the widespread genus contains over 300 species. The thallus (mycelium) typically consists of a highly branched network of multinucleate, septate, usually colorless hyphae. Many-branched conidiophores sprout on the mycelia, bearing individually constricted conidiospores. The conidiospores are the main dispersal route of the fungi, and often are green in color. Sexual reproduction involves the production of ascospores, commencing with the fusion of an archegonium and an antheridium, with sharing of nuclei. The irregularly distributed asci contain eight unicellular ascospores each.