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SAMPLE
Matrix: formulations Sample preparation: Finely powder tablets, weigh out amount equivalent to 20 mg enalapril maleate, suspend in 100 mL mobile phase, filter, inject a 5 jxL aliquot.
HPLCVARIABLES
Column: 250 X 4.6 12 fxm Hypersil C18 Mobile phase: MeCN.water 20:80 adjusted to pH 3.8 with acetic acid Flow rate: 1 Injection volume: 5 Detector: UV 215 for 3.5 min, then UV 275
CHROMATOGRAM
Simultaneous: hydrochlorothiazide
KEYWORDS
tablets
REFERENCE
el Walily, A.F.M.; Belal, S.F.; Heaba, E.A.; El Kersh, A. Simultaneous determination of enalapril maleate and hydrochlorothiazide by first-derivative ultraviolet spectrophotometry and high-performance liquid chromatography. J.Pharm.Biomed.Anal., 1995, 13, 851-856
SAMPLE
Matrix: formulations Sample preparation: Dissolve tablets in MeCN :1 mM pH 2 KH2PO4 50:50, centrifuge, inject a 50 JJLL aliquot of the supernatant.
HPLCVARIABLES
Column: 250 X 4.6 5 j u u m Spherisorb C8 Mobile phase: MeCN: buffer 35:65 (Buffer was 1 mM KH2PO4 adjusted to pH 2 with phosphoric acid.) Column temperature: 40 Flow rate: 2.5 Injection volume: 50 Detector: UV 215
CHROMATOGRAM
Retention time: 11
OTHER SUBSTANCES
tablets
HPLCVARIABLES
Column: 5 |xm Ultrasphere ODS Mobile phase: MeCN: 50 mM phosphate buffer 12:88, pH 3.2 Flow rate: 1 Detector: UV
CHROMATOGRAM Limit of detection: 50 nM KEYWORDS
rat
REFERENCE
Friedman, D.L; Amidon, G.L. Passive and carrier-mediated intestinal absorption components of two angiotensin converting enzyme (ACE) inhibitor prodrugs in rats: enalapril and fosinopril. Pharm.Res., 1989, 6, 1043-1047
Column: 250 X 4.6 Spherisorb 5 ODS-2 Mobile phase: n-Propanol:buffer 20:80 (Buffer was pH 3.0 phosphate buffer containing 0.4% triethylamine.) Flow rate: 1 Detector: UV 240
CHROMATOGRAM Retention time: 6 OTHER SUBSTANCES
Column: 300 X 3.9 jxBondapak phenyl Mobile phase: MeOH: water: 85% phosphoric acid 60:40:0.05 Column temperature: 30-40 Detector: UV 215-220
OTHER SUBSTANCES Also analyzed: lisinopril REFERENCE
Ranadive, S.A.; Chen, A.X.; Serajuddin, A.T. Relative lipophilicities and structural-pharmacological considerations of various angiotensin-converting enzyme (ACE) inhibitors. Pharm.Res., 1992, 9, 14801486