Patricia Marie A. de la Pea IV-A BSBT Semi-detailed Lesson Plan in Histology and Microtechniques I.

Objectives At the end of the discussion, the students must be able to: a. Define stratified epithelium; b. Describe the characteristics of a stratified epithelium; c. Distinguish the difference between the different types of stratified epithelia; and d. Give the importance of the functions of stratified epithelia in the human body. II. Subject Matter Topic: Stratified Epithelia Concept: a. Stratified epithelia are defined as two or more layers of cells in which only the basal layers of cells in which only the basal layer is attached to the basement membrane. b. In general, stratified epithelia are more protective in function than those of the simple variety. c. The three major types of stratified epithelia are stratified squamous epithelia, stratified cuboidal and columnar epithelia, and uroepithelium. References: http://ocw.tufts.edu/data/15/342520.pdf http://www.bio.davidson.edu/people/kabernd/BerndCV/Lab/EpithelialInfoWe b/Stratified%20Epithelium.html http://www.vetmed.vt.edu/education/curriculum/vm8304/lab_companion/his to-path/vm8054/labs/Lab4/Examples/exstrcub.htm http://microanatomy.net/epithelia/stratified_columnar.htm http://www.bio.davidson.edu/people/kabernd/BerndCV/Lab/EpithelialInfoWe b/Ciliated%20Columnar%20Epithelium.html Materials: Power point presentation Hand outs III. Procedure A. Routinary Activities 1. 2. 3. 4. 5. Greetings Prayer Attendance Review of the past lesson Motivation

Instructions for the motivational activity: Around the Body The class will be divided into two groups. The leader of each group begins by saying the name of any part of the body. The person next to him must then say another name that begins with the last letter of the word just given. Each person has a definite time limit (e.g. three seconds) and no names can be repeated. The players may use Filipino terms or scientific terms. The body parts include those that are seen or unseen inside or outside of the body.For example - First person: eyes, Second Person: siko, Third Person: olecranon. B. Lesson Proper: Stratified Epithelia- two or more layers of cells in which only the basal layers of cells in which only the basal layer is attached to the basement membrane. A. Stratified Squamous Epithelia Multilayered, stratified collection of cells undergoing constant formation, maturation and loss. Formed mitotically in the deepest or basal layer of the epithelium The cells are pushed more superficially, changing their shape from columnar or cuboidal to squamous. 1. Keratinized- found over the entire external body surface- the mucocutaneous layer such as the lips, nostrils, distal and canal, and the other surface of the tympanic membrane. 1. a. Stratum Spinosum- cells having spiny appearance. As these cells continue to divide and move, they begin to flatten and synthesize a dense, basophilic protein known as keratohylin, which fills the cells with keratin filaments. 2.b. Stratum Granulosum- synthesis and secretion of a waterproofing glycolipid. 3.b. Stratum Corneum- (non-living cells) tough, and hornlike and are continuously shed from the free surface. 2. Non-Keratinized - can be found where it may be subject to abrasion but is protected from drying. -located in the buccal cavity, oropharynx, laryngopharynx, esophagus, a part of the anal canal, the vagina, distal cervix of the uterus, distal urethra, conjunctive of the eye, cornea, vestibule of the nasal cavities.

They produce keratin, but the keratin does not fill the cell; they secrete glycolipid to a lesser extent and the nucleus is not lost until an entire cell desquamates at the free surface of the epithelium B. Stratified Cuboidal and Columnar Epithelium Characteristics of larger ducts of exocrine glands Can be found in the pancreatic, salivary gland and sweat gland ducts Function is not fully understood, but it may provide the duct with greater strength than other combinations of stratified cell types

C. Uroepithelium (Transitional Epithelium) Lines the free surfaces of the urinary tract, from the large, collecting ducts of the kidneys, through the ureters and bladders, to the initial or proximal part of the urethra Males: lines the urethra as far as the ejaculatory ducts Female: (urethra) uroepithelium extends as far as the urogenital diaphragm Appears to be six-cells thick and lines some organs capable of considerable distention and contraction When stratified: The cells flatten but their relative position in regard to adjacent cells is maintained by the presence of numerous desmosomes. When relaxed: The cells located deep (basally) are cuboidal and uninucleate and contain numerous ribosomes; hence, the cells are someone basophilic

Evaluation: I. Directions: Identify what layer of keratinized stratified squamous epithelia is being pointed in the picture.

II.

Directions: Identify what type of stratified epithelium.