Beruflich Dokumente
Kultur Dokumente
N
1 2
Sridhar1,
Tandon1,
Nirmala
Rao2
Department of Pedodontics and Preventive Dentistry, Manipal College of Dental Sciences, Manipal, India Department of Oral Pathology and Microbiology, Manipal College of Dental Sciences, Manipal, India
Date of Web Publication 30-Oct-2009 Sridhar N, Tandon S, Rao N. A comparative evaluation of DIAGNOdent with visual and radiography for detection of occlusal caries: An in vitro study. Indian J Dent Res 2009;20:326-31
Abstract
Background: The diagnosis of dental caries is fundamental to the practice of dentistry. Despite the fact that dental caries has declined considerably, it is still a problem of great importance. The reduction in caries prevalence has not occurred uniformly for all the surfaces. The greatest reduction was observed at lesions located on smooth surfaces, so that occlusal caries are most common in children. Aim: The purpose of this study is to determine the clinical efficacy of DIAGNOdent in detecting occlusal caries. Materials and Methods: A total number of 50 teeth were subjected for visual, radiographic, and DIAGNOdent examinations. All the three methods were compared to histology which is a gold standard. Results: The result obtained showed that DIAGNOdent is superior to visual and radiographic methods in diagnosing occlusal caries. Diagnosis is becoming more important as methods of treatment also evolve and expand to include a wider range of options. There is now a requirement for a higher quality of diagnosis than was needed before. When should fissure sealants be applied for preventive or therapeutic reasons? One school of thought maintains that it is more cost-effective to target sealants and place them only on teeth which are likely to become carious, rather than adopting a simpler but more expensive blanket policy which includes low risk teeth and individuals. The important point to stress, however, is that the success or failure of these selective approaches relies heavily on the quality of the original diagnosis of the presence and extent of caries. Accurate sensitive diagnosis is imperative if the patients are to be categorized into risk groups on the basis of their past disease status and present disease activity. It is also becoming financially important that practitioners are able to identify high risk individuals, since the amount and complexity of the preventive care invested in these patients will largely depend upon the results of the practitioners' diagnostic assessments. In recent years, however, clinicians have reported difficulties in diagnosing occlusal caries by visual examination alone. It was shown that visual examination performed worst in comparison to radiography, fiberoptic transillumination, and electric resistance. Current clinical diagnostic techniques exhibit many disadvantages, e.g. in case of the explorer, transfer of cariogenic microorganisms from one site to another, and damage to the integrity of the enamel surface promoting conditions for caries development. [1] Bitewing radiographs are a help in the detection of the noncavitated occlusal dental caries and combined with
clinical inspection, achieve in permanent teeth a higher accuracy than visual inspection, visual inspection with magnification and visual inspection with light pressure probing. [2] But they do not enhance detection of initial occlusal caries lesions in enamel of either permanent or deciduous teeth and they increase the risk of x-ray overexposure. Laser fluorescence seems to be promising for the detection and rectification of carious lesions on occlusal surfaces [3] and free smooth surfaces. [4] The laser fluorescence device DIAGNOdent has demonstrated promising results for the detection of occlusal caries in studies undertaken in vivo and in vitro in permanent teeth. [5],[6] One study dealt with diagnostic performance for deciduous teeth comparing two conventional methods with DIAGNOdent. [7] The present study was performed to evaluate the specificity and sensitivity of laser0 fluorescence using DIAGNOdent in detection of occlusal caries in comparison to visual and radiographic methods.
caries detection. In this study, probe A was used to examine the teeth [Figure 1]. Once the teeth were prophylactically cleaned and rinsed thoroughly, occlusal surfaces of test sites were examined using DIAGNOdent according to manufacturer's instructions. Probe A was used under cotton roll isolation and after air drying with an air syringe. The instrument was calibrated using ceramic mounting that was provided by manufacturer. Probe A was place perpendicular to the test site and rotated along the fissure to completely scan the area. Base line reading for each tooth was taken by placing the probe on sound tooth surface. Three measurements were taken and mean of them was considered as a final base line value. Moment reading that indicates amount of demineralization at specific area was checked at mesial pit, central pit, and distal pit. Peak value that gives maximum amount of demineralization was checked by scanning the entire occlusal surface. Three measurements were performed for each tooth and mean of them was taken. This value was then subtracted from base line value to attain the final value. Then scoring was given according to the cutoff limits given by the manufacturer [Table 3]. Histologic examination was performed after the teeth were examined by all the three methods. Teeth were mounted on methyl metha acrylate and were hemisectioned using 0.1 mm thick diamond saw mounted on microtome (Leica, Germany) in a mesio distal direction with a thickness of approximately 100 . The cut sections were mounted on glass slides with Canada balsam as a mounting medium. Sections were viewed under light microscope (Leica Dental Corp, Germany) with 40X magnification by a single examiner. Presence of caries in enamel or dentin was evaluated using Ekstrand criteria (1998) [6] [Figure 2],[Figure 3],[Figure 4],[Figure 5],[Figure 6],[Table 4].
When all the test teeth were examined in the present study with laser device DIAGNOdent, out of 50 teeth 44 (88%) were diagnosed as carious [Figure 10]. In in vitro studies done by Attrill and Ashley, [7] Antonnen et al., [15] Francescut and Lussi [16] found 100% clinical success of DIAGNOdent. But the conditions of the teeth selected were not explained clearly. Probably, the variation could also be due to the difference in sample size and selection criteria of teeth. In in vitro group in the present study out of 50, 22 teeth (84.6%) showed DIAGNOdent reading as D3 (dentin demineralization) and histology score as H2 (demineralization extended between 50% of enamel and one-third of dentin) [Figure 10]. However, in case of 13 teeth DIAGNOdent has over scored the caries where histology revealed no caries in four teeth and outer enamel caries in nine teeth. There are various causes for over scoring of teeth by DIAGNOdent. One prerequisite for DIAGNOdent is that the tooth surface to be examined should be carefully cleaned because the instrument is very sensitive to the presence of stains, deposits, and calculus, which may be registered as a change in enamel or dentin. Therefore, careful inspection of the tooth surface is recommended. The fundamental basis for detection and quantification of carious lesion by DIAGNOdent method is registration of altered physical characteristics of carious hard tissues relative to surrounding sound surfaces. The principal limitation of the method is that an increased reading could reveal any change in the physical properties of the tooth structure, such as caries, disturbed tooth development, or mineralization as well as deposits of calculus or organic material. Therefore, clinical experience is a fundamental prerequisite for using the instrument as an aid to detection of clinical caries. As mentioned before in this study DIAGNOdent has over scored 13 teeth for which histology revealed no visible caries. According to Ekstrand's [8] visual scoring system, the presence of discoloration, visible without air drying is an indicator of demineralization involving between 50% of the enamel and the outer third of dentin which is considered a 'sound site' at the dentine caries level. When discolored sites were evaluated by DIAGNOdent there was high tendency for these sites to be over scored, resulting in high rate of false positives. Nevertheless, great care was taken to clean the sites beforehand; it is unlikely that this is the reason. Recent evidence of studies conducted by Sheehy et al., [5] Francescut and Lussi, [16] Heinrich-weltzien et al. [17] also indicate that DIAGNOdent tends to over score discolored sites. At similar lesion depths, discolored fissures resulted in DIAGNOdent median values about 5-7 units higher than in opaque or nondiscolored fissures. Apart from this when DIAGNOdent was compared to visual and radiographic methods it has shown much superior results in diagnosing the caries. The comparisons obtained statistically in visual and DIAGNOdent were found to be significantly comparable. A total of 48 teeth, which were diagnosed as carious by visual method, also showed increased values of laser fluorescence. This reveals that there is a significant correlation between the visual and laser fluorescence in detection of occlusal caries. But in cases of stained occlusal surfaces if the treatment decision is solely based on DIAGNOdent reading there will be a tendency to overestimate the lesion stage and a higher risk of overtreatment. In such cases, visual examination is more appropriate to the clinician or at least the dentist using the laser fluorescence system must be aware of its limitations before reaching a treatment decision. Burin et al. [12] are also of the opinion that visual inspection alone is significantly better than DIAGNOdent with respect to specificity. Similarly DIAGNOdent has shown high sensitivity (100%) and acceptable specificity (44%) in detection of occlusal caries when compared to visual and radiographic methods. It has shown a positive predictive value of 76.2%. This high sensitivity and acceptable specificity was obtained also in some other studies conducted by Rocha et al. [15] and Bengston et al.[10] In contrast, Yaziki et al. [18] reported that DIAGNOdent has shown higher specificity values than sensitivity.
Conclusions
Caries prevention is critical in children, especially in developing countries where younger generations are acquiring more westernized dietary habits, which is expected to contribute to an increase in dental caries. Despite there is a reduction in prevalence of dental caries it is still a problem of great importance. The reduction of caries prevalence has not occurred uniformly for all the dental surfaces. Several studies have been undertaken with the objective of determining the most accurate and precise method for detection of
carious lesions among conventional and new methods. In this study, the efficacy of laser device (DIAGNOdent) in detection of caries was evaluated with histological gold standard. The following conclusions were made from this study:
1. 2. 3. 4. 5.
The laser fluorescence device DIAGNOdent has shown more superior results in diagnosing the caries which is almost comparable to histological gold standard. DIAGNOdent has shown very good sensitivity and specificity in diagnosing the caries. Visual and laser fluorescence (DIAGNOdent) both have shown similar performance in terms of sensitivity and specificity. Radiography performed poorly in diagnosing occlusal caries compared to other three methods. However, it has shown accurate results in reference to dentinal caries. The laser device DIAGNOdent can be used as a valuable and preventive diagnostic tool as an adjunct to visual examination.
1. 2. 3. 4.
Even though DIAGNOdent has excellent sensitivity and specificity, in some cases it has over scored the teeth. The cutoff limits that were given by the manufacturer are often confusing. So more precise values are required to exactly locate the carious lesion. The laser device DIAGNOdent is not able to detect the amount of demineralization. An adequate learning and experience is required for its precise use before considering it as a reliable diagnostic method.