Filters Frits

Filters & Frits
Using a scale to count Upchurch Scientific frits in Oak Harbor, WA.
IDEX Health & Science introduces Upchurch Scientific precolumn and inline very high pressure (VHP) filters on page 159
156
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We are pleased to present our 2011/2012 catalog, which includes Innovadyne, Ismatec, Isolation Technologies, Rheodyne, Systec, and Upchurch Scientific products. In this catalog, you will find all the critical fluidic products and high-quality components youve come to expect from IDEX Health & Science. This catalog has a new look and feel, with a new organization to all of our products. Now, you will be able to find all of our fittings in one chapter, with separate chapters for products like tubing, valves, pumps, etc. And, as always, we have many new products including some very innovative UHPLC fittings and accessories scattered throughout the catalog that we hope will prove useful to you. We appreciate your business and continued loyalty to our family of products and services and thank you for trusting IDEX Health & Science with your liquid handling needs. Your feedback is valuable, so please let us know if there are ways we can be of greater service.
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Inlet Solvent Filters

General Use Inlet Solvent Filters
Large surface areas prevent pump cavitation Disposable 2 m, 10 m, and 20 m pore sizes available General use and prep filters for higher flow applications
Filters & Frits
Application Note
Why Use An Inlet Solvent Filter?
To filter out particulate matter from the solvent that may otherwise damage expensive hardware. (Use a 10 m or 20 m version for this purpose. The A-309 and A-230A filters have an added Bottom of the Bottle feature to help draw solvent to within 1/8 of the bottom of your solvent bottle.) To prevent particulates originating from the sparging system from entering the mobile phase reservoir and to help disperse the sparging gas efficiently. (Use a 2 m filter for this purpose.) To hold your tubing in place at the bottom of the bottle. (Most stainless steel filter options work best for this purpose.) Important Note: It is usually a good idea to change the inlet filter as part of your semi-annual or annual preventative maintenance program.
It is good practice to filter your solvents to prevent pump damage. Upchurch Scientific 316 stainless steel filters provide that protection. Because filters should be changed periodically, we make it easy to replace them without tools. For those filters using a plastic fitting, the tubing can be reconnected by finger tightening the fitting into the new filter. The filters with stems allow easy insertion into the inlet tubing.
1.27 (3.23 cm)
1.12 (2.84 cm)
A-242 2 m with P-100 Fingertight Nut
A-302 10 m
0.50 (1.27 cm)
for 1/16 ID tubing 0.50 (1.27 cm) 2.3 (5.84 cm)
for 1/8 OD tubing
1.12 (2.84 cm)
1.12 (2.84 cm)
0.88 (2.24 cm)
A-302A 10 m with XP-315 Flangeless Fittings

for 1/8 OD tubing
A-310 10 m
0.50 (1.27 cm)
for 1/8 ID tubing 0.50 (1.27 cm)
A-225A 20 m with P-315/P-300N Flangeless Fittings

for 1/8 OD tubing
0.68 (1.73 cm)
A-230A 20 m with XU-655 Flangeless Fittings

for 1/4 OD tubing
1.00 (2.54 cm)
Part No.
Description
Porosity
Material
For Tubing Size
Includes
Max. Suggested Flow Rate*
Price
GENERAL USE INLET SOLVENT FILTERS For Analytical HPLC

A-240 A-241 A-242 A-243 A-228 A-302 A-302A A-309 A-231A A-310 A-225 A-225A A-226A A-227A A-230A A-311 A-311A Inlet Solvent Filter with One-Piece Fitting A-240, 5-pack Inlet Solvent Filter with One-Piece Fitting A-242, 5-pack Inlet Solvent Filter with stem Inlet Solvent Filter with stem Inlet Solvent Filter with Flangeless Fittings Inlet Solvent Filter with stem Inlet Solvent Filter with Flangeless Fittings Inlet Solvent Filter with stem Inlet Solvent Filter with stem Inlet Solvent Filter with Flangeless Fittings Inlet Solvent Filter with Flangeless Fittings Inlet Solvent Filter with Flangeless Fittings Inlet Solvent Filter with Flangeless Fittings Inlet Solvent Filter with stem Inlet Solvent Filter with Flangeless Fittings 10 m 10 m 2 m 2 m 2 m 10 m 10 m 10 m 20 m 10 m 20 m 20 m 10 m 10 m 20 m 10 m 10 m PCTFE, SST PCTFE, SST PCTFE, SST PCTFE, SST SST SST PCTFE, SST SST PCTFE, SST SST SST PCTFE, SST PCTFE, SST PCTFE, SST PCTFE, SST SST PCTFE, SST PCTFE, SST PCTFE, SST 1/8 OD 1/8 OD 1/8 OD 1/8 OD 1/8 ID 1/16 ID 1/8 OD 1/16 ID 3/16 OD 1/8 ID 1/16 ID 1/8 OD 5/16 OD 1/4 OD 1/4 OD 1/16 ID 1/8 OD 1/8 OD 1/8 OD (1) P-100 (5) P-100 (1) P-100 (5) P-100 (1) XP-315 (1) XP-132 (1) P-315, (1) P-300N (1) XU-662 (1) XU-655 (1) XU-655 (1) XP-315 40 mL/min 40 mL/min 10 mL/min 10 mL/min 80 mL/min 40 mL/min 40 mL/min 40 mL/min 100 mL/min 40 mL/min 100 mL/min 100 mL/min 100 mL/min 100 mL/min 100 mL/min 100 mL/min 100 mL/min $ 23.97 120.36 22.64 114.24 54.15 17.26 21.77 22.60 $ 30.37 21.77 $ 20.63 30.37 63.20 63.20 65.90 52.52 58.70 $ 90.76 90.76
For Waters Analytical HPLC Systems
For Preparative HPLC Systems
INLET SOLVENT FILTER KITS

A-200 Inlet Solvent Filter Kit 10 m A-201 Inlet Solvent Filter Kit 2 m *Maximum suggested flow rates are determined by porosity and surface area. FEP Tubing (1/8 OD x 1/16 ID x 3 length), (5) P-100, (5) A-220 FEP Tubing (1/8 OD x 1/16 ID x 3 length), (5) P-100, (5) A-222
157
Filters & Frits
Inlet Solvent Filters

UHMWPE Bottom-of-the-Bottle Solvent Filters
Replaceable filter cup Economical Materials of construction: UHMWPE, ETFE Versions for 1/16 and 1/8 OD tubing
Stainless Steel Bottom-of-the-Bottle Solvent Filters

Draws solvent from within 1/8 of the bottom of the bottle Replaceable stainless steel filter cups Versions for 1/8 and 3/16 OD tubing Materials of construction: PEEK, ETFE and 316 Stainless Steel
Patented Stainless Steel Bottom-of-the-Bottle Solvent Filter Assemblies feature a 2 m or 10 m replaceable stainless steel filter cup and a design that allows solvent to be drawn from within 1/8 of the bottom of your solvent bottle. The filter cups are inexpensive and easy to replace, making this an economical, trouble-free choice.
The design of the UHMWPE solvent filters allows tubing to pass through to the bottom of the filter cup, enabling the filter to draw solvent from within 0.10 (2.5 mm) of the bottom of your solvent bottle. Please Note: UHMWPE is a hydrophobic material. To establish proper surface wetting, you may need to prime the filter with methanol or acetonitrile.
0.55 (1.40 cm)
1.70 (4.32 cm)
1.50 (3.81 cm)
A-550 Bottom-of-the-Bottle Inlet Solvent Filter Maximum Flow Rates: 2 m up to 10 mL/min. 10 m up to 40 mL/min.
0.50 (1.27 cm)
A-446 Solvent Filter Maximum flow up to 5 mL/min

0.44 (1.12 cm)
PEEK Bottom-of-the-Bottle Solvent Filters

Most recommended filtering unit 100% PEEK polymer construction Easy operation no fittings required
Top Seller SEE STARRED PRODUCTS

Part No. Description A-550 A-551 A-552 A-553 A-520x A-522x SST Filter Assembly, with A-520 filter cup SST Filter Assembly, with A-522 filter cup SST Filter Assembly with A-520 filter cup SST Filter Assembly with A-522 filter cup SST Replacement Solvent Filter Cups, 10-pk SST Replacement Solvent Filter Cups, 10-pk Porosity 10 m 2 m 10 m 2 m 10 m 2 m For Tubing Size 1/8 OD 1/8 OD 3/16 OD 3/16 OD Includes (1) XP-130 (1) XP-130 (1) XP-132 (1) XP-132 Price $ 60.50 60.50 60.50 60.50 26.37 26.37
These biocompatible filters are made from 100% PEEK polymer, including the two built-in PEEK frits. The bottom frit (2 m or 10 m) will draw solvents from within 0.080 (2.0 mm) of the bottom of the solvent bottle. The 2 m frit on the side may be used for a 1/8 OD helium sparging line. To use, simply press fit your appropriately sized fluoropolymer tubing firmly into the top holes. Thats it!
Port for solvent intake line Port for your sparging line (optional use) Filters and disperses incoming sparging gas (optional use)
BOTTOM-OF-THE-BOTTLE SOLVENT FILTERS
Filters solvent from the bottom of your solvent bottle
BIOCOMPATIBLE BOTTOM-OF-THE-BOTTLE SOLVENT FILTERS

A-445 A-446 A-427 UHMWPE Filter Assembly 10 m UHMWPE Filter Assembly 10 m UHMWPE Replacement 10 m Solvent Filter Cups, 5-pk PEEK Filter PEEK Filter PEEK Filter, for small-neck (GL-38) bottles PEEK Filter, for small-neck (GL-38) bottles PEEK Filter PEEK Filter PEEK Filter PEEK Filter PEEK Filter, for small-neck (GL-38) bottles PEEK Filter, for small-neck (GL-38) bottles 2 m 2 m 2 m 10 m 10 m 10 m 2 m 10 m 2 m 10 m 1/16 OD 1/8 OD (1) XP-245 (1) XP-345 $ 17.99 17.99 22.49
0.79 (2.01 cm)
0.79 (2.01 cm)
BIOCOMPATIBLE BOTTOM-OF-THE-BOTTLE SOLVENT FILTERS

A-435 A-436 A-437 A-438 1/8 OD 3/16 OD 1/8 OD 1/8 OD 1/8 OD 3/16 OD 1/16 OD 1/16 OD 1/16 OD 1/16 OD $ 23.66 23.66 23.66 23.66 23.66 23.66 21.93 21.93 21.93 21.93
1.10 (2.79 cm)
0.91 (2.31 cm)
A-435 Bottom-of the-Bottle Filter
A-438 Bottom-of the-Bottle Filter

(for small neck bottles)
A-440 A-441 A-450 A-451 A-452 A-453
Maximum Flow Rate up to 30 mL/min
158
Precolumn and Inline Filters

Very High Pressure Filters
Inline and precolumn configurations available Pressure rated to 15,000 psi (1,035 bar) Low internal volume
Filters & Frits
The two new VHP Filters are available for UHPLC applications, where filtering particulates from the system is extremely important to prolonging the life of sub-2 m particle columns. Place the Inline VHP Filter between the pump and injection valve to prolong the life of the injection valve rotor seal by removing any particulates generated by wear of the pump seal. The small volume of the Inline Filter minimizes the introduction of dispersion in the system. Select from 0.2 or 0.5 m porosity rating for both filter styles. The unique Precolumn VHP Filter offers additional column protection designed to connect directly into the 10-32 coned port of your column or other piece of hardware. Incorporating a spring loaded, self-adjusting tube, the Precolumn Filter adjusts to any port depth, eliminating any possibility of dead volume. The Precolumn Filter can be reused in multiple ports and a good connection is ensured every time. The replacement product for the Precolumn Filter is easy to use and includes a fresh piece of stainless steel tubing, which extends the lifetime of the assembly. Connect 1/16 OD and 10-32 coned fittings from pages 17, 19 and 20. The VHP Inline and Precolumn Filters include five replaceable frit assemblies, which can also be purchased separately in packs of ten.
0.485 (1.23 cm)
0.94 (2.39 cm)
0.375 (0.95 cm) 1.34 (3.40 cm)
VHP-500 Inline VHP Filter
VHP-550 Precolumn VHP Filter
Part No.
Description Inline VHP Filter Inline VHP Filter Replacement Inline VHP Frit Replacement Inline VHP Frit Precolumn VHP Filter Precolumn VHP Filter Replacement Precolumn VHP Frit Assembly Replacement Precolumn VHP Frit Assembly
Porosity 0.5 m 0.2 m 0.5 m 0.2 m 0.5 m 0.2 m 0.5 m 0.2 m
Material SST SST SST SST SST/PK SST/PK SST SST
Includes (5) VHP-501 (5) VHP-506 N/A N/A (5) VHP-551 (5) VHP-556 N/A N/A
Threads 10-32 Coned 10-32 Coned N/A N/A 10-32 Coned 10-32 Coned N/A N/A
Volume 1.2 L 1.1 L 0.60 L 0.54 L 1.9 L 1.8 L 1.9 L 1.8 L
Qty. ea. ea. 10-pk 10-pk ea. ea. 10-pk 10-pk
Price $ 282.00 282.00 214.72 214.72 332.00 332.00 322.08 322.08
VHP FILTERS
VHP-500 VHP-505 VHP-501x VHP-506x VHP-550 VHP-555 VHP-551x VHP-556x
159
Filters & Frits
ColumnSaver Precolumn Filter

Economical protection for your analytical column Finger tight to 6,000 psi (414 bar) without wrenches or other tools Lower dead volume than conventional filters with holders Universal connection compatible with all column manufacturers fittings Compatibility with stainless steel or PEEK tubing
The PEEK ColumnSaver Precolumn Filters offer all the protection of conventional precolumn filters at a third of the cost. With its convenient direct connect design, changeover time is measured in seconds and no tools or wrenches are needed. The ColumnSaver PEEK body contains a HiFlo stainless steel or titanium filter element and is designed for maximum filtration of particulate matter with minimal dead volume or backpressure. The stainless steel ColumnSaver Precolumn Filter offers similar benefits as the PEEK version, but it is especially designed for use in UHPLC applications. Manufactured with an all-stainless steel body and featuring a 2 m stainless steel frit, the stainless steel ColumnSaver offers precolumn filtration protection in those applications where inline pressures can reach as high as 20,000 psi (1,379 bar). (Please Note: Because of the design of this product, it needs to be wrenchtightened in place.)
0.30 (0.76 cm) 1.06 (2.69 cm) 0.30 (0.76 cm) 1.02 (2.59 cm)
9085-20-10 ColumnSaver Precolumn Filter
9085-20-SS ColumnSaver Precolumn Filter
Related Products
For micro-scale chromatographic applications where flow rates and sample sizes are significantly reduced from traditional analytical scale chromatography try our M-550 or M-560 Precolumn MicroFilters on page 164.
Application Note
The ColumnSaver can also be used as a convenient, fingertight inline filter when used with a 1/16 union. As soon as an increase in backpressure is detected, simply remove and dispose of the ColumnSaver unit and replace it with a new one. The direct-connect design is compatible with 1/16 10-32 internal fitting ports featured on many standard, high pressure stainless steel unions.
Threads 10-32 Coned 10-32 Coned 10-32 Coned 10-32 Coned Material PEEK PEEK SST PEEK Swept Volume 3.1 L 3.1 L 2.04 L 3.1 L Pressure Rating 6,000 psi (414 bar) 6,000 psi (414 bar) 20,000 psi (1,379 bar) 6,000 psi (414 bar) Qty. 10-pk 10-pk ea. ea. Price $ 150.00 125.30 41.93 19.53
Part No. 9085-05-10 9085-20-10 9085-20-SS 9085-20T
Description ColumnSaver Precolumn Filter, with SST frit ColumnSaver Precolumn Filter, with SST frit ColumnSaver Precolumn Filter, with SST frit ColumnSaver Precolumn Filter, with Titanium frit
Porosity 0.5 m 2 m 2 m 2 m
COLUMNSAVER PRECOLUMN FILTERS
SST = Stainless Steel
160

Precolumn Filters
0.5 m or 2 m porosity frits available Great column protection Feature stainless steel bodies and polymer-encased stainless steel frits
Filters & Frits
Inline Solvent Filters

Excellent for general purpose inline use 2 m and 20 m porosity frits available
Upchurch Scientific Standard Precolumn Filters have 0.020 (0.50 mm) diameter thru-holes and 8 distribution cones for minimal band spreading and mixing. They feature stainless steel bodies, and pressure rated to 6,000 psi (414 bar) and both accept 1/16 OD tubing and standard 10-32 threaded high pressure fittings. With a 0.010 (0.25 mm) diameter thru-hole, our A-318 Filter has one of the lowest swept volumes (0.61 L*) of any analytical HPLC precolumn filter available, ensuring maximum protection with very little band broadening. Pressure rated to 6,000 psi (414 bar).
0.49 (1.24 cm)
Placed between the pump and sample injection valve, Inline Solvent Filters trap particles released through normal piston seal wear. Without an inline filter, particles can be flushed through your systems tubing to the sample injection valve, resulting in valve damage and further system contamination. These filters are pressure rated to 6,000 psi (414 bar) and use a 2 m or 20 m stainless steel frit. An 8 distribution cone helps spread the flow of the mobile phase over the entire surface of the frit, while the 0.050 (1.3 mm) diameter thru-hole allows virtually unrestricted solvent flow rates.
0.49 (1.24 cm)
0.94 (2.39 cm)
0.94 (2.39 cm)
A-315 Precolumn Filter

0.49 (1.24 cm)
A-101 2 m SST frit
A-313 Inline Solvent Filter
A-224 20 m SemiPrep SST frit
0.94 (2.39 cm)
0.49 (1.24 cm)
0.94 (2.39 cm)

0.48 (1.22 cm)
A-102 0.5 m SST frit
A-314 Inline Solvent Filter
A-100 2 m SST frit
1.00 (2.54 cm)
Related Products
Fittings
A-102 0.5 m SST frit
All filters on these two pages have 10-32 internal threads for 1/16 OD tubing, conveniently allowing the use of Fingertight Fittings. Use any of the allpolymer 10-32 Fingertight Fittings found on pages 11, 13 and 15 with these filters. For the stainless steel filters, you may also use the 10-32 stainless steel fittings on page 10.
Frits

Part No. A-315 A-316 A-318 A-101x A-102x Description Solvent Filter Assembly Solvent Filter Assembly Solvent Filter Assembly Replacement Frits, Stainless Steel, 10-pk Replacement Frits, Stainless Steel, 10-pk Porosity 2 m 0.5 m 0.5 m 2 m 0.5 m 20 m 2 m 2 m 20 m Threads
Any of the polymer-ring encased frits from pages 167 171 with an overall diameter of 0.247 to 0.254 can be used with the filters on this page and the biocompatible precolumn filters.
Includes (1) A-101 (1) A-102 (1) A-102 (1) A-224 (1) A-100 Swept Volume* 1.4 L 1.3 L 0.84 L 0.74 L 0.61 L 12.3 L 4 L 1.4 L 9.7 L Pressure Rating 6,000 psi (414 bar) 6,000 psi (414 bar) 6,000 psi (414 bar) N/A N/A 6,000 psi (414 bar) 6,000 psi (414 bar) N/A N/A Price $ 52.86 52.86 67.97 31.99 31.99 $ 52.86 52.86 31.99 3.70
PRECOLUMN FILTERS
10-32 Coned 10-32 Coned 10-32 Coned 10-32 Coned 10-32 Coned
INLINE SOLVENT FILTERS

A-313 Solvent Filter Assembly A-314 Solvent Filter Assembly A-100x Replacement Frits, Stainless Steel, 10-pk A-224 Replacement Frits, Stainless Steel, ea. *Swept volumes include/reflect theoretical frit volume values.
161
Filters & Frits

Biocompatible Inline Filters
0.5 m and 2 m versions available Features 100% PEEK flow path
Biocompatible Precolumn Filters

Pre-assembled with either 0.5 m or 2 m porosity frits Great column protection Feature PEEK bodies and PCTFE-surrounded PEEK frits
Upchurch Scientific Biocompatible Precolumn Filters have 0.020 (0.50 mm) diameter thru-holes and 8 distribution cones for minimal band spreading and mixing. The bodies of these filters are manufactured from biocompatible PEEK polymer and are pressure rated to 5,000 psi (345 bar). These filters are designed for use with 1/16 OD tubing, which can be connected to these filters using standard Fingertight fittings.
0.62 (1.57 cm)
0.94 (2.39 cm)
Upchurch Scientific A-430 and A-431 Inline Filters consist of a stainless steel body and two PEEK end fittings, one with a built-in 2 m or 0.5 m PEEK frit. Both filters are pressure rated to 6,000 psi (414 bar), with maximum recommended flow rates of 25 mL/min for the A-430 Filter and 10 mL/min for the A-431 Filter. And, you get the added benefit of biocompatibility since all wetted surfaces are PEEK. When you need to replace the frit, simply dispose of the end fitting that contains the frit and replace it with a new one. The end fittings have 10-32 female ports to connect 1/16 OD or smaller tubing using standard Fingertight fittings.
A-430 Biocompatible Analytical Inline Filter
A-700 2 m PEEK frit A-355 PEEK Precolumn Filter
End fitting with 100% PEEK frit
Stainless steel body

0.99 (2.51 cm)
0.62 (1.57 cm)
End fitting
0.94 (2.39 cm)
0.56 (1.42 cm)
A-701 0.5 m PEEK frit A-356 PEEK Precolumn Filter
Application Note
Increase the Life of Your Column
Why use a Precolumn Filter when there is a frit at the head of the column itself? Changing the column frit is extremely difficult to do without disturbing the column packing. A precolumn filter provides relatively inexpensive insurance against column damage, and changing its frit is easy. A Precolumn Filter placed between the sample injection valve and the HPLC column protects the column from particles originating in the sample and from pump and valve seal wear.
Note
Whats the Difference Between Precolumn & Inline Filters?
You may have noticed that the bodies of Precolumn and Inline Filters look similar, and as such, you may have wondered what the differences are. Because Precolumn Filters, by definition, are typically placed in a volumesensitive area immediately preceding the column, these filters usually feature smaller thru-holes, smaller frit diameters, and lower overall internal volumes. In contrast, Inline Filters are often placed where the internal volume is not as critical and where longer life and less fluid restriction is more important.

Part No. A-355 A-356 A-700 A-701 A-430 A-431 A-428x A-429x Description Solvent Filter Assembly, Biocompatible Solvent Filter Assembly, Biocompatible Replacement Frit, PEEK Polymer, ea. Replacement Frit, PEEK Polymer, ea. Biocompatible Filter Assembly Biocompatible Filter Assembly PEEK Filter End Fittings, Black PEEK body, 10-pk PEEK Filter End Fittings, Natural PEEK body, 10-pk Porosity 2 m 0.5 m 2 m 0.5 m 2 m 0.5 m 0.5 m 2 m Threads 10-32 Coned 10-32 Coned 10-32 Coned 10-32 Coned 10-32 Coned 10-32 Coned Includes (1) A-700 (1) A-701 (1) A-429 (1) A-428 Swept Volume* 1.4 L 1.3 L 0.74 L 0.61 L 7.1 L 5.9 L 5.7 L 6.9 L Pressure Rating 5,000 psi (345 bar) 5,000 psi (345 bar) N/A N/A 6,000 psi (414 bar) 6,000 psi (414 bar) N/A N/A Price $ 54.77 54.77 7.77 7.77 $ 23.61 23.61 56.43 56.43
BIOCOMPATIBLE PRECOLUMN FILTERS
BIOCOMPATIBLE INLINE FILTERS
* Swept volumes include/reflect theoretical frit volume values.
162

Semi-Prep Inline Filters
Designed for high flow applications
Filters & Frits
Biocompatible Semi-Prep Inline Filters

Versions for 1/16, 1/8, 3/16, 1/4 and 5/16 OD tubing 100% PEEK flow path
The A-330 High Pressure Semi-Prep Filter has a swept volume of 223 L and has been tested with water at flow rates up to 100 mL/min. At this rate the filter adds only 50 psi (3 bar) additional back pressure.
1.14 (2.90 cm)
1.05 (2.67 cm)
Biocompatible Semi-Prep Filters consist of a stainless steel body, two PEEK end fittings, and a separate PEEK frit. The A-410 and A-411 are pressure rated to 6,000 psi (414 bar), while the A-510 and A-511 are for lower pressure applications and have threaded ports designed to connect large bore tubing. These filters are ideal for many higher flow analytical, semi-prep and preparative applications. Best of all, if the filter becomes clogged, simply unscrew the assembly, remove the frit and replace it. As an added benefit and for greater flexibility, the frits used with each of these assemblies are interchangeable.
0.050 (0.13 cm) thru-hole 0.50 (1.27 cm) 10 m Stainless Steel Frit
A-330 High Pressure Semi-Prep Inline Filter
Shown with standard 10-32 stainless steel nuts and ferrules (not included)
Iso-Prep Filters
Economical protection for preparative HPLC columns and injectors SFC & HPLC compatible Ideal for columns with inner diameters from 21.2 to 50 mm ID
This preparative inline filter holder with replaceable frit will help protect your semi-prep column against contamination.
1.61 (4.09 cm)
1.34 (3.40 cm)
1.00 (2.54 cm)
A-410 Biocompatible Semi-Prep Inline Filter
0.88 (2.24 cm)
1020-20 Iso-Prep Filter
0.56 (1.42 cm) Can use either 0.5 m or 2 m Stainless Steel Frit (included) Description Semi-Prep Filter Assembly Semi-Prep Filter Assembly Stainless Steel Frits, Natural ETFE ring Stainless Steel Frits, Natural ETFE ring Stainless Steel Frits, Natural ETFE ring 21.2 mm Filter Holder 21.2 mm Filter Holder 21.2 mm Replacement Filter 21.2 mm Replacement Filter Biocompatible Filter Assembly Biocompatible Filter Assembly Biocompatible Filter Assembly Biocompatible Filter Assembly PEEK Frit, Green PCTFE ring PEEK Frit, Natural PCTFE ring PEEK Frit, Natural PCTFE ring Porosity 10 m 10 m 10 m 2 m 20 m 0.5 m 2 m 0.5 m 2 m 2 m 10 m 5 m 5 m 2 m 10 m 5 m Threads 10-32 Coned 5/16-24 Flat Bottom N/A N/A N/A 10-32 Coned 10-32 Coned N/A N/A 10-32 Coned 10-32 Coned 5/16-24 Flat Bottom 1/2-20 Flat Bottom N/A N/A N/A Includes (1) A-331 (1) A-331 N/A N/A N/A (1) 7031-05 (1) 7031-20 N/A N/A (1) OC-802 (1) OC-803 (1) OC-805 (1) OC-805 N/A N/A N/A

Swept Volume* 223 L 235 L 142 L 122 L 152 L 203 uL 196 uL 122 uL 115 uL 89 L 103 L 89 L 141 L 46 L 57 L 50 L Pressure Rating 7,500 psi (517 bar) 3,500 psi (207 bar) N/A N/A N/A 8,000 psi (552 bar) 8,000 psi (552 bar) 8,000 psi (552 bar) 8,000 psi (552 bar) 6,000 psi (414 bar) 6,000 psi (414 bar) 500 psi (34 bar) 250 psi (17 bar) N/A N/A N/A Qty. ea. ea. 10-pk 10-pk 10-pk ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. Price $ 81.47 81.47 118.47 118.47 118.47 $ 279.93 279.93 20.65 17.85 $ 96.66 96.66 96.66 96.66 15.24 15.24 10.54
Part No. A-330 A-360 A-331x A-332x A-337x 1020-05 1020-20 7031-05 7031-20 A-410 A-411 A-510 A-511 OC-802 OC-803 OC-805
SEMI-PREP INLINE FILTERS
ISO-PREP FILTERS
BIOCOMPATIBLE SEMI-PREP INLINE FILTERS
*Swept volumes include/reflect theoretical frit volume values.
163
Filters & Frits
MicroFilters
Precolumn MicroFilters
Inline MicroFilters
100% biocompatible PEEK polymer option available As low as 109 nL void volume Two versions: direct connect 1/32 OD tubing or use MicroTight tubing sleeves for 70 520 m OD capillary tubing
Direct connects to columns with 10-32 threads Total void volume of 0.5 L Two versions: direct connect 1/16 OD tubing or use MicroTight tubing sleeves for 70 520 m OD capillary tubing
Upchurch Scientific Inline MicroFilters protect your column from particles originating in the mobile phase or sample, or from pump seal and sample injection valve wear. These filters have a 0.006 (150 m) thru-hole. Choose the M-520 with a 0.5 m 100% PEEK frit or the M-135 1 m stainless steel filtering screen version, to connect to capillary tubing using the MicroTight tubing sleeves (page 19). You may also directly connect 1/32 OD tubing using the M-525 which contains a 0.5 m PEEK frit.
The Precolumn MicroFilters directly connect into your microbore or analytical column. Total theoretical void volume is only 0.5 L (includes frit volume) and the PEEK tubing used in the assembly of these units has a 0.005 (125 m) ID, virtually eliminating any mixing of the sample with the mobile phase.
Adjusts to port depth F-125 Micro Fingertight
Tubing Seal Ferrule Assembly
PEEK Spacer
P-416 Nut
M-520 Inline MicroFilter Assembly
PEEK Frit
M-120 Filter End Fitting
MicroTight Tubing Sleeve

(not included)
Filter Body Replaceable Frit Holder M-500 Precolumn MicroFilter Assembly

(Includes indicated products)
F-172 Ferrule
MicroTight Tubing Sleeve

(not included)
M-525 Inline MicroFilter Assembly
Application Note
The Mini MicroFilter (page 165) and Inline MicroFilter can be used to pack capillary tubing. Simply place one of these filters on the effluent side of the capillary tubing, then slurry pack. Once packed, place a filter at the head of the tubing. This creates a reliable capillary column without fusing the silica to make frits or pressing filter paper inside the capillary tubing.
For Tubing Size MicroTight Tubing Sleeve MicroTight Tubing Sleeve 1/32" OD MicroTight Tubing Sleeve MicroTight Tubing Sleeve 1/32" OD MicroTight Tubing Sleeve MicroTight Tubing Sleeve 1/16" OD 1/16" OD N/A N/A N/A Includes (5) M-130, (2) F-125 (5) M-120, (2) F-125 (5) M-140, (2) F-126 N/A N/A N/A (5) C-425, (1) F-172, (1) P-416 (5) A-735, (1) F-172, (1) P-416 (5) C-425, (1) F-132, (1) P-416 (5) A-735, (1) F-132, (1) P-416 N/A N/A N/A Swept Volume* Pressure Rating 109 nL 240 nL 240 nL 216 nL 85 nL 216 nL 0.5 L 0.5 L 0.5 L 0.5 L 216 nL 101 nL 101 nL 4,000 psi (276 bar) 4,000 psi (276 bar) 4,000 psi (276 bar) N/A N/A N/A 4,000 psi (276 bar) 4,000 psi (276 bar) 4,000 psi (276 bar) 4,000 psi (276 bar) N/A N/A N/A Qty. ea. ea. ea. 10-pk 10-pk 10-pk ea. ea. ea. ea. 10-pk 10-pk 10-pk Price $ 81.24 88.84 93.84 $ 111.73 83.16 173.20 $ 98.56 98.56 98.56 98.56 $ 76.29 64.81 64.81

Part No. M-135 M-520 M-525 M-120x M-130x M-140x M-500 M-510 M-550 M-560 Description Inline MicroFilter Assembly, SST Screen Inline MicroFilter Assembly, PEEK Frit Inline MicroFilter Assembly, PEEK Frit End-Fittings, Black, with PEEK Frit End-Fittings, Natural, with SST Filter Screen End-Fittings, Natural, with PEEK Frit Precolumn MicroFilter Assembly, SST Frit Precolumn MicroFilter Assembly, PEEK Frit Precolumn MicroFilter Assembly, SST Frit Precolumn MicroFilter Assembly, PEEK Frit Porosity 1 m 0.5 m 0.5 m 0.5 m 1 m 0.5 m 0.5 m 0.5 m 0.5 m 0.5 m 0.5 m 2 m 0.5 m
INLINE MICROFILTERS
REPLACEMENT INLINE MICROFILTER END-FITTINGS
PRECOLUMN MICROFILTER ASSEMBLIES
REPLACEMENT PRECOLUMN MICROFILTER FRITS

A-735x PEEK Frits, 0.045 x 0.031 x 0.192 C-420x SST Frits, 0.038 x 0.028 x 0.192 C-425x SST Frits, 0.038 x 0.028 x 0.192 SST = Stainless Steel * Swept volumes include/reflect theoretical frit volume values.
164
MicroFilters
Mini MicroFilters
Total volume as low as 10 nL Conductive version for CEC and mass spectrometry applications Three versions available: direct connect 1/32 OD or 360 m OD tubing and a variety of capillary sizes using MicroTight tubing sleeves (70 520 m)
Filters & Frits
Upchurch Scientific Inline Mini MicroFilter Assemblies filter effectively with internal volumes low enough to ensure reliable chromatographic results even at nanoliter flow rates! Internal volumes of these encapsulated filters are as low as 85 nL with the micro-screen and 10 nL to 22 nL with the frit disc option. Apply voltage to the stainless steel filter holder body of the Conductive Mini MicroFilter for applications such as mass spectrometry and CEC analysis. The voltage is conducted through to the stainless steel portion of the 1 m NanoFilter Capsule and on to the fluid stream. Try our Insulating Mounting Bracket on page 39 to apply voltage easily and more safely.
M-530 Mini MicroFilter Assembly
M-534 Conductive Mini MicroFilter Assembly
Replaceable Filter Capsule Filter Capsule Holder
F-112 Ferrule
Tubing P-416 Nut M-542 Mini MicroFilter Assembly Stainless Steel Filter Screen
(Not included)
Note
Because of the size-specific nature of the ferrules included with each Mini MicroFilter assembly, please note that these ferrules are not interchangeable with other MicroFerrules for different tubing sizes.
Filter Capsule Color Identification
M-121
M-122
M-123
M-124
M-125
M-126

Part No. M-530 M-531 M-532 M-533 M-537 M-538 M-542 M-543 M-547 M-548 M-534 Description Mini MicroFilter Assembly Mini MicroFilter Assembly Mini MicroFilter Assembly Mini MicroFilter Assembly Mini MicroFilter Assembly Mini MicroFilter Assembly Mini MicroFilter Assembly Mini MicroFilter Assembly Mini MicroFilter Assembly Mini MicroFilter Assembly Conductive Mini MicroFilter Assembly Porosity 2 m 1 m 2 m 1 m 1 m 1 m 2 m 1 m 1 m 1 m 1 m Porosity 1 m 2 m 1 m 2 m 1 m 1 m 1 m 2 m 1 m 1 m 1 m Frit Type SST Screen SST Screen SST Screen SST Screen SST Frit Ti Frit SST Screen SST Screen SST Frit Ti Frit SST Frit Frit Type SST Screen SST Screen SST Screen SST Screen SST Frit Ti Frit SST Screen SST Screen SST Frit Ti Frit SST Frit For use with Tubing MicroTight tubing sleeves MicroTight tubing sleeves 360 m OD 360 m OD 360 m OD 360 m OD 1/32 (790 m) OD 1/32 (790 m) OD 1/32 (790 m) OD 1/32 (790 m) OD 360 m OD For Use With M-530 and M-531 M-530 and M-531 M-532 and M-533 M-532 and M-533 M-537 and M-538 M-537 and M-538 M-542 and M-543 M-542 and M-543 M-547 and M-548 M-548 M-534
M-128
M-131
M-132
M-133
M-134
Includes (5) M-122, (2) F-172, (2) P-416 (5) M-121, (2) F-172, (2) P-416 (5) M-124, (2) F-152, (2) P-416BLK (5) M-123, (2) F-152, (2) P-416BLK (5) M-125, (2) F-152, (2) P-416BLK (5) M-126, (2) F-152, (2) P-416BLK (5) M-132, (2) F-112, (2) P-416 (5) M-131, (2) F-112, (2) P-416 (5) M-133, (2) F-112, (2) P-416 (5) M-134, (2) F-112, (2) P-416 (5) M-128, (2) F-152, (2) P-416BLK Material PEEK PEEK PEEK PEEK PEEK PEEK PEEK PEEK PEEK PEEK SST/PEEK
Swept Volume 85 nL 85 nL 85 nL 85 nL 10 nL 10 nL 97 nL 97 nL 22 nL 22 nL 10 nL Swept Volume 85 nL 85 nL 85 nL 85 nL 10 nL 10 nL 85 nL 85 nL 10 nL 10 nL 10 nL
Pressure Rating 4,000 psi (276 bar) 4,000 psi (276 bar) 4,000 psi (276 bar) 4,000 psi (276 bar) 4,000 psi (276 bar) 4,000 psi (276 bar) 4,000 psi (276 bar) 4,000 psi (276 bar) 4,000 psi (276 bar) 4,000 psi (276 bar) 4,000 psi (276 bar) Qty. 2-pk 2-pk 2-pk 2-pk 2-pk 2-pk 2-pk 2-pk 2-pk 2-pk 2-pk
Price $ 155.47 155.47 155.47 155.47 198.59 223.45 155.47 155.47 198.59 223.45 254.32 Price $ 33.12 33.12 33.12 33.12 54.48 66.97 33.12 33.12 54.48 66.97 80.35
MINI MICROFILTER ASSEMBLY
REPLACEMENT MINI MICROFILTER CAPSULES

Part No. Description M-121 Filter Capsule M-122 Filter Capsule M-123 Filter Capsule M-124 Filter Capsule M-125 NanoFilter Capsule M-126 NanoFilter Capsule M-131 Filter Capsule M-132 Filter Capsule M-133 NanoFilter Capsule M-134 NanoFilter Capsule M-128 Conductive NanoFilter Capsule SST = Stainless Steel; Ti = Titanium
165
Filters & Frits
Specialty Filters
Frit-In-A-Ferrule
Seals and filters simultaneously Less expensive and more convenient than traditional inline filter systems Available in both Flangeless and Super Flangeless versions
P-373
Now you can filter at any point in your system where 1/16 or 1/8 OD tubing is used in a flat-bottom 1/4-28, M6 or 5/16-24 connection. The Upchurch Scientific Frit-In-A-Ferrule product line is designed to seal and filter simultaneously by incorporating a frit into the body of a flat-bottom ferrule. This simple design allows you to eliminate traditional inline filters and reduce the number of additional connections in your system.
0.19 (0.48 cm) 0.19 (0.48 cm) 0.16 (0.41 cm) 0.19 (0.48 cm)
P-270
P-275 P-372
P-372 Flangeless Frit-In-A-Ferrule

for 1/8 OD tubing
P-276 Super Flangeless Frit-In-A-Ferrule

for 1/16 OD tubing
Disposable Sample Filters

Excellent system protection Ultra-low hold-up volume 0.5 m and 2 m porosity
Related Products
Nuts that can be used with the Frit-In-A-Ferrule line:
Super Flangeless, pages 21 23 and 30 31 Flangeless, pages 24 27 and 30
B-100 and B-101 Disposable Sample Filters
These Disposable Sample Filters are designed to remove particles from analytical HPLC samples. The polypropylene holder incorporates a 1/32 thick, 1/8 diameter stainless steel frit, which causes very little back pressure. To use, just attach one of these filters onto the end of any standard luer syringe, such as our B-310 found on page 155.

Part No. P-270x P-271x P-272x P-273x P-274x P-275x P-276x P-370x P-371x P-372x P-373x P-374x P-375x Description Super Flangeless, Natural PEEK, SST lock ring Super Flangeless, Black PEEK, SST lock ring Flangeless, Green PCTFE Flangeless, Blue PCTFE Super Flangeless, Natural PEEK, SST lock ring Super Flangeless, Black PEEK, SST lock ring Super Flangeless, Red ETFE, SST lock ring Super Flangeless*, Natural PEEK, SST lock ring Super Flangeless*, Black PEEK, SST lock ring Flangeless, Green PCTFE Flangeless, Blue PCTFE Super Flangeless*, Natural PEEK, SST lock ring Super Flangeless*, Black PEEK, SST lock ring Porosity 2 m 0.5 m 2 m 0.5 m 2 m 0.5 m 10 m 2 m 0.5 m 2 m 0.5 m 2 m 0.5 m 2 m 0.5 m Frit Material SST SST SST SST PEEK PEEK SST SST SST SST SST PEEK PEEK SST SST Frit Diameter 0.062 0.062 0.062 0.062 0.046 0.046 0.062 0.094 0.094 0.094 0.094 0.094 0.094 Frit Thickness 0.062 0.062 0.062 0.062 0.030 0.030 0.062 0.062 0.062 0.062 0.062 0.042 0.042 Swept Volume 0.74 L 0.61 L 0.74 L 0.61 L 0.20 L 0.16 L 0.90 L 1.69 L 1.41 L 1.69 L 1.41 L 1.15 L 0.96 L Maximum Pressure 2,500 psi (172 bar) 2,500 psi (172 bar) 2,000 psi (138 bar) 2,000 psi (138 bar) 2,500 psi (172 bar) 2,500 psi (172 bar) 2,500 psi (172 bar) 2,500 psi (172 bar) 2,500 psi (172 bar) 500 psi (34 bar) 500 psi (34 bar) 2,500 psi (172 bar) 2,500 psi (172 bar) Qty. 10-pk 10-pk 10-pk 10-pk 10-pk 10-pk 10-pk 10-pk 10-pk 10-pk 10-pk 10-pk 10-pk 100-pk 100-pk Price $ 79.00 79.00 64.43 64.43 92.44 92.44 77.95 $ 79.00 79.00 64.43 64.43 92.44 92.44 $ 168.58 168.58
FRIT-IN-A-FERRULE FOR 1/16" OD TUBING
FRIT-IN-A-FERRULE FOR 1/8" OD TUBING
DISPOSABLE HPLC SAMPLE FILTERS

B-100 Disposable Filters B-101 Disposable Filters *The 1/8 Super Flangeless versions cannot be used in M6 ports.
166
Frits
PEEK Frits
Inert, biocompatible and metal-free Uniform porosity, longer filtration life Sealing rings manufactured from PCTFE
Filters & Frits
2 m Semi-Prep PEEK Frits

A-346 A-712-02
0.620 (1.57 cm) diameter 0.070 (0.18 cm) thick
Patented Upchurch Scientific PEEK Frits offer exceptionally uniform porosity. This property ensures longer filtration life and consistent frit-to-frit swept volumes. The PEEK polymer frit discs are biocompatible and inert to most solvents, making them well-suited for bioanalytical applications. PEEKs robust properties make these products suitable for low and high pressure applications. Disc rings, included on most PEEK frits, are made of PCTFE and are slightly thicker than the frit disc, providing enhanced sealing and excellent chemical resistance. PCTFE surrounded PEEK frits can be used up to 80 C, and PEEK frits alone are a good choice for applications up to 100 C.
0.750 (1.91 cm)
0.740 (1.88 cm) 0.070 (0.18 cm)
OC-802
0.560 (1.42 cm)
0.5 m PEEK Frits

A-701
0.250 (0.64 cm) 0.062 (0.16 cm) diameter 0.062 (0.16 cm) thick
A-703
0.250 (0.64 cm)
A-705
0.092 (0.23 cm) diameter 0.062 (0.16 cm) 0.250 (0.64 cm) thick 0.125 (0.32 cm) diameter 0.062 (0.16 cm) thick
Application Note
Frit Volume
The term frit volume refers to the volume of the various fluid pathways that comprise the matrix of a frit. A standard frit is a mass of small particles fused together through a controlled process of compression and heat. Because of their shape, there are gaps between the fused particles. Fluid makes its way through these gaps, creating a pathway from one side of the frit to the other. (See the diagram, below, where the white circles represent frit particles, and the black area represents the void between the particles.)
A-707
A-709
0.200 (0.51 cm)
A-711
0.062 (0.16 cm) diameter 0.062 (0.16 cm) 0.200 thick (0.51 cm) 0.125 (0.32 cm) diameter 0.062 (0.16 cm) thick
A-713-02
A-735
0.740 (1.88 cm) 0.062 (0.16 cm)
2 m PEEK Frits
A-700
A-702
0.250 (0.64 cm)
A-704
Generally, when the frit particles increase in size, the frits porosity increases as well. The larger the particles, the larger the gaps between particles. Cumulatively, these gaps comprise what is known as frit volume. Using gravimetric determination, it has been experimentally shown that the total volume of any given frit may range from 18%30%, depending upon the porosity of the frit. Frit volume is calculated by determining what the mass of the frit would be if it were a solid block of material of equal size. Then the solid mass of the frit is multiplied by the percentage assigned to the porosity to determine the theoretical frit volume. 18% for 0.2 m frits 20% for 0.5 m frits 24% for 2 m frits 26% for 5 m frits 28% for 10 m frits 30% for 20 m frits From a chromatographic perspective, its important to know the volume of the frit used in your system. It is possible for a frit to negatively impact your chromatography if the total frit volume is too large and if it is placed in an area through which the sample will pass. To avoid frit-related problems like band broadening and loss of resolution, most inline filters placed after the sample introduction point (e.g., between the injection valve and the column) are smaller in size and porosity than inline filters that are placed in areas before the sample is introduced into the flow path (e.g., between the pump and the injection valve).
A-706
A-708
0.200 (0.51 cm)
A-710
Order Online
WWW. IDEX-HS .COM
167
Filters & Frits
Frits
PEEK Frits (cont.)

5 m and 10 m PEEK Frits
A-715-02 A-720
0.250 (0.64 cm) 0.740 (1.88 cm) 0.072 (0.18 cm) 0.197 (0.5 cm) diameter 0.070 (0.18 cm) thick 0.560 (1.42 cm) 0.125 (0.32 cm) diameter 0.070 (0.18 cm) thick
Note
The thickness dimension in the part drawings and the pricing tables represents the thickness of the frit disc not the frit ring. Frit rings are often slightly thicker to ensure a proper seal. When tightened into a filter holder the ring compresses to the thickness of the frit disc. The manufacturing process may cause some slight color variance in our PEEK frits. This does not affect their quality or performance. Frit dimensions are approximate. Actual batch-to-batch frit dimensions may vary slightly.
A-722
0.250 (0.64 cm)
OC-803
Related Products
Any 0.247 to 0.254 diameter frit (including polymer ring) can be used with the filters on page 161.
OC-804
OC-805
0.460 (1.17 cm) diameter 0.058 (0.15 cm) thick 0.060 (0.15 cm)
0.305 (0.77 cm)
0.560 (1.42 cm)
PAT and PEEK Frit Equivalents

PAT (PEEK alloyed with PTFE) frits are no longer available for purchase. PEEK frits are an excellent alternative to PAT frits. The table below shows the PEEK frit equivalents for the now obsoleted PAT frit line. Still unsure of what size to order? Please contact us for more information.
Obsolete PAT Frits 5 m 9000-0432 9000-0435 9000-0451 6145-3500 PEEK Frit Equivalent 5 m OC-813 OC-813 OC-815 N/A PEEK Frit Equivalent 10 m A-720 A-722 A-720 A-722 N/A OC-803
OC-813
0.250 (0.64 cm)
OC-815
0.375 (0.95 cm)
OC-820
0.250 (0.64 cm)

Part No. Porosity 2 m 0.5 m 2 m 0.5 m 2 m 0.5 m 2 m 0.5 m 2 m 0.5 m 2 m 0.5 m 0.5 m 0.5 m 2 m 2 m 10 m 10 m 10 m 2 m 10 m 10 m 5 m 5 m 5 m 10 m Disc Diameter 0.062 (0.16 cm) 0.062 (0.16 cm) 0.091 (0.23 cm) 0.092 (0.23 cm) 0.125 (0.32 cm) 0.125 (0.32 cm) 0.188 (0.48 cm) 0.195 (0.5 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.125 (0.32 cm) 0.125 (0.32 cm) 0.740 (1.88 cm) 0.045 (0.11 cm) 0.620 (0.16 cm) 0.740 (1.88 cm) 0.740 (1.88 cm) 0.125 (0.32 cm) 0.197 (0.5 cm) 0.460 (1.17 cm) 0.460 (1.17 cm) 0.194 (0.49 cm) 0.460 (1.17 cm) 0.183 (0.46 cm) 0.293 (0.74 cm) 0.183 (0.46 cm) Disc Thickness 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.031 (0.08 cm) 0.070 (0.18 cm) 0.070 (0.18 cm) 0.072 (0.18 cm) 0.070 (0.18 cm) 0.070 (0.18 cm) 0.070 (0.18 cm) 0.072 (0.18 cm) 0.059 (0.15 cm) 0.058 (0.15 cm) 0.064 (0.16 cm) 0.062 (0.16 cm) 0.065 (0.17 cm) Ring OD 0.250 (0.64 cm) 0.250 (0.64 cm) 0.250 (0.64 cm) 0.250 (0.64 cm) 0.250 (0.64 cm) 0.250 (0.64 cm) 0.250 (0.64 cm) 0.250 (0.64 cm) 0.200 (0.51 cm) 0.200 (0.51 cm) 0.200 (0.51 cm) 0.200 (0.51 cm) N/A 0.192 (0.23 cm) 0.750" (1.91 cm) N/A N/A 0.250 (0.64 cm) 0.250 (0.64 cm) 0.560 (1.42 cm) 0.560 (1.42 cm) 0.305 (0.77 cm) 0.560 (1.42 cm) 0.250 (0.64 cm) 0.375 (0.95 cm) 0.250 (0.64 cm) Ring Material PCTFE PCTFE PCTFE PCTFE PCTFE PCTFE PCTFE PCTFE PCTFE PCTFE PCTFE PCTFE N/A PCTFE PCTFE N/A N/A PCTFE PCTFE PCTFE PCTFE PCTFE PCTFE PCTFE PCTFE PCTFE Frit Volume 0.7 L 0.6 L 1.7 L 1.4 L 3.0 L 2.4 L 7.1 L 6.1 L 0.7 L 0.6 L 3.0 L 2.5 L 87.4 L 0.2 L 85.5 L 115.2 L 142.1 L 4.2 L 9.9 L 46.4 L 57.2 L 8.4 L 41.1 L 7.2 L 17.8 L 7.8 L Qty. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. Price $ 7.77 7.77 7.77 7.77 7.77 7.77 7.77 7.77 7.77 7.77 7.77 7.77 12.74 7.77 $ 10.59 12.74 12.74 7.77 7.77 15.24 15.24 7.89 10.54 7.77 12.76 7.41
PEEK FRITS
A-700 A-701 A-702 A-703 A-704 A-705 A-706 A-707 A-708 A-709 A-710 A-711 A-713-02 A-735 A-346 A-712-02 A-715-02 A-720 A-722 OC-802 OC-803 OC-804 OC-805 OC-813 OC-815 OC-820
SEMI-PREP PEEK FRITS
168
Frits
Titanium Frits
Excellent alternative to stainless steel PEEK or PCTFE polymer rings
Filters & Frits
Note
While all of the frits listed in this chapter are capable of withstanding high pressures (>5,000 psi or 345 bar), the actual pressure holding capability of each frit is usually dependent on the filter body in which it is placed.
Titanium is a biocompatible alternative to stainless steel. Our 0.2 m, 0.5 m and 2 m porosity titanium frits are surrounded by PEEK or PCTFE polymer rings for enhanced sealing. The dimensions of most of these frits make them suitable replacement frits for Upchurch Scientific filters and guard columns.
0.2 m Titanium Frits

A-501
2 m Titanium Frits
A-503
0.062 (0.16 cm) diameter 0.094 (0.24 cm) thick 0.247 (0.63 cm) 0.188 (0.48 cm) diameter 0.062 (0.16 cm) thick
A-502
A-108
0.250 (0.64 cm)
A-109
0.062 (0.16 cm) diameter 0.062 (0.16 cm) 0.250 (0.64 cm) thick 0.094 (0.24 cm) diameter 0.062 (0.16 cm) thick
A-110
0.248 (0.63 cm)
0.248 (0.63 cm)
0.254 (0.65 cm)
A-504
A-505
A-506
A-111
A-342-02
A-419
0.250 (0.64 cm)
0.250 (0.64 cm)
0.730 (1.85 cm)
A-507
A-421
0.062 (0.16 cm)
0.200 (0.51 cm)
0.5 m Titanium Frit

A-131
0.250 (0.64 cm)
Part No.
Porosity 2 m 2 m 2 m 2 m 0.5 m 2 m 2 m 2 m 0.2 m 0.2 m 0.2 m 0.2 m 0.2 m 0.2 m 0.2 m
Disc Diameter 0.062 (0.16 cm) 0.094 (0.24 cm) 0.188 (0.48 cm) 0.125 (0.32 cm) 0.188 (0.48 cm) 0.730 (1.85 cm) 0.062 (0.16 cm) 0.125 (0.32 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.188 (0.48 cm) 0.118 (0.30 cm 0.038 (0.10 cm) 0.038 (0.10 cm) 0.038 (0.10 cm)
Disc Thickness 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.094 (0.24 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.028 (0.07 cm) 0.028 (0.07 cm) 0.028 (0.07 cm)
Ring OD 0.250 (0.64 cm) 0.250 (0.64 cm) 0.254 (0.65 cm) 0.250 (0.64 cm) 0.250 (0.64 cm) N/A 0.200 (0.51 cm) 0.200 (0.51 cm) 0.248 (0.63 cm) 0.248 (0.63 cm) 0.247 (0.63 cm) 0.250 (0.64 cm) 0.060 (0.15 cm) 0.192 (0.49 cm) 0.125 (0.32 cm)
Ring Material PCTFE PCTFE PCTFE PCTFE PCTFE N/A PCTFE PCTFE PEEK PEEK PCTFE PCTFE PCTFE PCTFE PCTFE
Frit Volume 0.7 L 1.6 L 7.1 L 2.7 L 5.6 L 93.6 L 0.7 L 2.7 L 0.6 L 0.8 L 5.1 L 2 L 0.1 L 0.1 L 0.1 L
Qty. 10-pk 10-pk 10-pk ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea. ea.
Price $ 62.11 62.11 62.11 7.50 8.00 9.10 9.98 7.50 8.00 8.00 8.00 8.00 8.00 8.00 8.00
TITANIUM FRITS
A-108x A-109x A-110x A-111 A-131 A-342-02 A-419 A-421 A-501 A-502 A-503 A-504 A-505 A-506 A-507
169
Filters & Frits
Frits
Stainless Steel Frits

Upchurch Scientific 316 Analytical-scale Stainless Steel Frits are available in 0.5 m or 2 m porositythe most common HPLC filtration ratings. Each frit includes a PCTFE or PEEK polymer sealing ring. Many of the frits shown have the common 0.250 (0.64 cm) and 0.254 (0.64 cm) ODs, which allow them to be used in the precolumn and inline filters found starting on page 160. Choose the larger diameter faces and/or larger porosity frits for faster flow rates. Choose frits with a smaller diameter face and/or smaller porosity for applications sensitive to extra flow path volume.
Application Note
To Clean Or Not To Clean?
It is rarely worth the time and effort to clean frits, given the relatively low cost of replacements. Furthermore, cleaning may leave some debris embedded in the frit pores. If the washed frit is accidently returned to your instrument in a reverse orientation, any remaining debris could be flushed out and deposited further down the fluid path. If this frit is being used as a column head frit, the debris may be washed directly onto the column bed.
0.5 m Stainless Steel Frits

A-102
0.250 (0.64 cm)
2 m Stainless Steel Frits

A-418
0.094 (0.24 cm) diameter 0.062 (0.16 cm) thick 0.200 (0.51 cm) 0.062 (0.16 cm) diameter 0.062 (0.16 cm) thick
A-103
0.062 (0.16 cm) diameter 0.062 0.250 (0.16 cm) (0.64 cm) thick
A-100
A-101
0.094 (0.24 cm) diameter 0.062 0.250 (0.16 cm) thick (0.64 cm) 0.062 (0.16 cm) diameter 0.062 (0.16 cm) thick
A-417
0.250 (0.64 cm)
C-128-31
0.125 (0.32 cm)
C-140-30
C-409
0.062 (0.16 cm)
A-420
0.200 (0.51 cm)
C-401
C-402
0.254 (0.65 cm)
C-411
0.250 (0.64 cm)
C-425
C-407
0.062 (0.16 cm)
C-408
C-420
0.062 (0.16 cm)
0.192 (0.49 cm)

Part No. A-100x A-101x A-102x A-103x A-417 A-418 A-420 C-128-31 C-140-30x C-401x C-402x C-407x C-408x C-409x C-411 C-420x C-425x Porosity 2 m 2 m 0.5 m 0.5 m 2 m 0.5 m 2 m 0.5 m 0.5 m 2 m 2 m 2 m 2 m 0.5 m 0.5 m 2 m 0.5 m Disc Diameter 0.094 (0.24 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.094 (0.24 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.125 (0.32 cm) 0.038 (0.10 cm) 0.188 (0.48 cm) 0.125 (0.32 cm) 0.188 (0.48 cm) 0.038 (0.10 cm) 0.038 (0.10 cm) 0.038 (0.10 cm) 0.250 (0.64 cm) 0.038 (0.10 cm) 0.038 (0.10 cm) Disc Thickness 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.028 (0.07 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.028 (0.07 cm) 0.028 (0.07 cm) 0.028 (0.07 cm) 0.062 (0.16 cm) 0.028 (0.07 cm) 0.028 (0.07 cm) Ring OD 0.250 (0.64 cm) 0.250 (0.64 cm) 0.250 (0.64 cm) 0.250 (0.64 cm) 0.200 (0.51 cm) 0.200 (0.51 cm) 0.200 (0.51 cm) 0.125 (0.32 cm) 0.254 (0.65 cm) 0.250 (0.64 cm) 0.254 (0.65 cm) 0.062 (0.16 cm) 0.125 (0.32 cm) 0.062 (0.16 cm) N/A 0.192 (0.49 cm) 0.192 (0.49 cm) Ring Material PEEK PEEK PEEK PEEK PCTFE PCTFE PCTFE PEEK PCTFE PEEK PEEK PCTFE PEEK PCTFE N/A PCTFE PCTFE Frit Volume 1.7 L 0.7 L 0.6 L 1.4 L 0.7 L 0.6 L 3.0 L 0.1 L 6.5 L 3.0 L 7.8 L 0.1 L 0.1 L 0.1 L 10.0 L 0.1 L 0.1 L Qty. 10-pk 10-pk 10-pk 10-pk ea. ea. ea. ea. 10-pk 10-pk 10-pk 10-pk 10-pk 10-pk ea. 10-pk 10-pk Price $ 31.99 31.99 31.99 31.99 5.68 5.68 5.68 5.07 31.99 31.99 31.99 54.71 54.71 54.71 2.54 64.81 64.81
STAINLESS STEEL FRITS
170
Frits
Stainless Steel Semi-Prep Frits
Many of these frits come complete with a PCTFE, ETFE or PTFE resin sealing ring. Choose from 2 m, 5 m, 10 m and 20 m filtration porosities and a range of diameters to match your intended flow rate and filtration requirements.
Filters & Frits
20 m Semi-Prep Stainless Steel Frits

A-120
A-122
A-224

A-332 A-343
0.250 (0.64 cm)
0.254 (0.65 cm)
0.254 (0.65 cm)
A-337
A-345
0.880 (2.24 cm)
0.750 (1.91 cm)
0.880 (2.24 cm)
0.750 (1.91 cm)

A-105 A-106
Stainless Steel Frit Discs

A-107
0.189 (0.48 cm) diameter 0.062 (0.16 cm) thick 0.750 (1.91 cm) 0.062 (0.16 cm)
A-337-02
0.250 (0.64 cm)
0.254 (0.65 cm)
A-331
0.880 (2.24 cm)
C-413
0.250 (0.64 cm)
C-414
C-415
0.062 (0.16 cm)
0.375 (0.95 cm)
0.062 (0.16 cm)
0.500 (1.27 cm)
0.062 (0.16 cm)
Note

Part No. A-105x A-106x A-107x A-120x A-122x A-224 A-331x A-332x A-337x A-343 A-345 A-335-02 A-336-02 A-337-02 C-400x C-412 C-413 C-414 C-415 Porosity 10 m 10 m 10 m 20 m 20 m 20 m 10 m 2 m 20 m 2 m 20 m 2 m 10 m 20 m 2 m 5 m 10 m 2 m 2 m Disc Diameter 0.062 (0.16 cm) 0.094 (0.24 cm) 0.189 (0.48 cm) 0.125 (0.32 cm) 0.188 (0.48 cm 0.188 (0.48 cm 0.750 (1.91 cm) 0.750 (1.91 cm) 0.750 (1.91 cm) 0.625 (1.59 cm) 0.625 (1.59 cm) 0.750 (1.91 cm) 0.750 (1.91 cm) 0.750 (1.91 cm) 0.250 (0.64 cm) 0.250 (0.64 cm) 0.250 (0.64 cm) 0.375 (0.95 cm) 0.500 (1.27 cm Disc Thickness 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm) 0.062 (0.16 cm)
Frits without the polymer rings cannot be used with our standard Precolumn and Inline Filter assemblies.
Ring OD 0.250 (0.64 cm) 0.250 (0.64 cm) 0.254 (0.65 cm) 0.250 (0.64 cm) 0.254 (0.65 cm) 0.254 (0.65 cm) 0.880 (2.24 cm) 0.880 (2.24 cm) 0.880 (2.24 cm) 0.750 (1.91 cm) 0.750 (1.91 cm) N/A N/A N/A N/A N/A N/A N/A N/A Ring Material PCTFE PCTFE PCTFE PCTFE PCTFE PTFE ETFE ETFE ETFE PCTFE PCTFE N/A N/A N/A N/A N/A N/A N/A N/A Frit Volume 0.9 L 2.0 L 9.1 L 3.7 L 9.7 L 9.7 L 141.9 L 141.9 L 152 L 112.6 L 93.5 L 107.7 L 125.7 L 134.7 L 12 L 13 L 14 L 26.9 L 47.9 L Qty. 10-pk 10-pk 10-pk 10-pk 10-pk ea. 10-pk 10-pk 10-pk ea. ea. ea. ea. ea. 10-pk ea. ea. ea. ea. Price $ 57.03 57.03 57.03 32.55 32.55 3.70 118.47 118.47 118.47 8.55 10.05 $ 2.87 2.87 2.87 24.49 2.54 2.54 3.19 3.19
SEMI-PREP STAINLESS STEEL FRITS
STAINLESS STEEL FRIT DISCS (NO POLYMER RINGS)
171
Filters & Frits
Bottle Caps and Plugs
Bottle Caps
Extremely simple no threaded ports or fittings Manufactured from ETFE and Polypropylene
Application Note
A self-regulating sparging system can help reduce helium consumption and improve pump performance. Set this up by pressing your tubing through the appropriate holes in your bottle cap and attaching each line to a filter. Sparge your mobile phase with an inert gas (preferably helium) for 1520 minutes. Then reduce the outlet pressure of the sparging gas to a maximum of 5 psi (0.34 bar) and insert a plug (A-626 or A-628) into the remaining port of the cap. The sparging gas will shut off once the incoming pressure equals the pressure inside the reservoir. As the mobile phase is consumed and the internal pressure lowers, sparging gas will enter to keep the system pressurized and degassed. Please Note: If gas leaks while pressurizing the bottle, try removing the sealing ring from the bottle, as it sometimes interferes with the sealing of Upchurch Scientific bottle caps. One concern with sparging systems is the possibility of solvent backing up the sparging inlet line. This can occur if the gas tank completely evacuates with the regulating valves open, creating a vacuum in the tubing. Solvent backup may damage sparging system components and cause crosscontamination of mobile phase reservoirs. To help prevent solvent backup, install the CV-3010 Inline Check Valve (page 151) along the tubing line that runs between the gas supply and the solvent bottle. For a more efficient degassing system, please see the Systec HPLC Vacuum Degassing Systems on pages 177 178. Please see the Quick-Stop Luer Check Valve on page 151 for another solvent inlet Application Note.
If you are looking for a bottle cap that is quick and easy to use, but still allows many connection options, we have just what you need! The Bottle Caps fit standard GL-45 (1 L) or smaller-neck GL-38 (4 L) glass bottles. Each cap has three holes. With two of the holes you simply push your tubing straight through. The third hole, with a luer taper, can be used for a number of options. Any male luer (such as a luer-lock syringe) will fit snugly in this hole, or you can use the A-626 or A-627 Plug. Exceptions are the A-610 and A-610B Bottle Caps. Please see the note below right.
Holes for 1/8 OD tubing Additional hole for luer A-620 GL-45 Bottle Cap
for 1/8 tubing
Holes for 1/8 OD tubing Additional hole for luer A-622 GL-38 Bottle Cap
for 1/8 tubing
Note
The A-610 and A-610B Bottle Caps have a slightly different configuration than the other caps. One hole accepts 3/16 OD tubing, the typical size used with a Waters system. The remaining two holes accept 1/8 OD tubing. Unlike the other caps, the A-610 does not have a tapered luer hole. If desired, use our A-628 Plug or A-629 Filter Plug for one of the 1/8 holes.
Bottle Cap Plugs and Adapters

Use the A-626 Bottle Cap Plug to seal the third tapered luer hole found in most Upchurch Scientific Bottle Caps. Or, use the A-628 Plug to seal any unused 1/16 or 1/8 bottle cap holes. Alternatively, try the A-627 or A-629 Filter Bottle Cap Plug to cap an unused hole in your bottle cap. The 20 m stainless steel frit in these products prevents foreign matter from contaminating your solvent while leaving the bottle open to the atmosphere, thus allowing fluid to be pulled out without creating a vacuum (generally not used with sparging applications). All plug bodies are manufactured from ultrahigh molecular weight polyethylene (UHMWPE). Our P-600 Bottle Cap Adapter is designed to allow the use of 1/32 OD tubing with our caps. Just insert the Adapter into a tubing cap hole, then connect your 1/32 OD tubing with our F-126S or F-126H MicroTight Fitting (page 18).
Related Products
To ensure a tight seal, use Upchurch Scientific fluoropolymer tubing with these bottle caps (pages 70 73).

Part No. A-610 A-610B A-620 A-620B A-630 A-630B A-622 A-632 Description for 3/16 OD tubing, Red for 3/16 OD tubing, Blue for 1/8 OD tubing, Red for 1/8 OD tubing, Blue for 1/16 OD tubing, Red for 1/16 OD tubing, Blue for 1/8 OD tubing, Black for 1/16 OD tubing, Black Bottle Cap Plug for luer hole, UHMWPE Filter Bottle Cap Plug for luer hole, UHMWPE with 20 m stainless steel frit Bottle Cap Plug for 1/16, 1/8 or 3/16 hole, UHMWPE Filter Bottle Cap Plug for 1/16, 1/8 or 3/16 hole, UHMWPE with 20 m stainless steel frit Bottle Cap Adapter for 1/8 hole, PEEK to connect 1/32 OD tubing using F-126 or F-126H fitting (page 18) Price $ 22.12 22.12 22.12 22.12 22.12 22.12 $ 22.12 22.12 $ 4.34 6.24 4.34 6.24 11.46
BOTTLE CAPS FOR GL-45, 1 L BOTTLES
20 m stainless steel frit A-626 Bottle Cap Plug A-629 Filter Bottle Cap Plug P-600 Bottle Cap Adapter
BOTTLE CAPS FOR GL-38, 4 L BOTTLES
BOTTLE CAP PLUGS AND ADAPTER

A-626 A-627 A-628 A-629 P-600
to connect 1/32 OD tubing
172

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Filters & Frits

Using a scale to count Upchurch Scientific frits in Oak Harbor, WA.

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Inlet Solvent Filters

Filters & Frits

1.27 (3.23 cm)

1.12 (2.84 cm)

A-242 2 m with P-100 Fingertight Nut

for 1/16 ID tubing 0.50 (1.27 cm) 2.3 (5.84 cm)

for 1/8 OD tubing

1.12 (2.84 cm)

1.12 (2.84 cm)

0.88 (2.24 cm)

A-302A 10 m with XP-315 Flangeless Fittings

for 1/8 ID tubing 0.50 (1.27 cm)

A-225A 20 m with P-315/P-300N Flangeless Fittings

0.68 (1.73 cm)

A-230A 20 m with XU-655 Flangeless Fittings

1.00 (2.54 cm)

For Tubing Size

Max. Suggested Flow Rate*

GENERAL USE INLET SOLVENT FILTERS For Analytical HPLC

For Waters Analytical HPLC Systems

For Preparative HPLC Systems

INLET SOLVENT FILTER KITS

Filters & Frits

Inlet Solvent Filters

Stainless Steel Bottom-of-the-Bottle Solvent Filters

1.70 (4.32 cm)

1.50 (3.81 cm)

A-446 Solvent Filter Maximum flow up to 5 mL/min

PEEK Bottom-of-the-Bottle Solvent Filters

Top Seller SEE STARRED PRODUCTS

BOTTOM-OF-THE-BOTTLE SOLVENT FILTERS

Filters solvent from the bottom of your solvent bottle

BIOCOMPATIBLE BOTTOM-OF-THE-BOTTLE SOLVENT FILTERS

0.79 (2.01 cm)

0.79 (2.01 cm)

BIOCOMPATIBLE BOTTOM-OF-THE-BOTTLE SOLVENT FILTERS

1.10 (2.79 cm)

0.91 (2.31 cm)

A-435 Bottom-of the-Bottle Filter

A-438 Bottom-of the-Bottle Filter

A-440 A-441 A-450 A-451 A-452 A-453

Maximum Flow Rate up to 30 mL/min

Precolumn and Inline Filters

Filters & Frits

0.485 (1.23 cm)

0.94 (2.39 cm)

0.375 (0.95 cm) 1.34 (3.40 cm)

VHP-500 Inline VHP Filter

VHP-550 Precolumn VHP Filter

Porosity 0.5 m 0.2 m 0.5 m 0.2 m 0.5 m 0.2 m 0.5 m 0.2 m

Material SST SST SST SST SST/PK SST/PK SST SST

Volume 1.2 L 1.1 L 0.60 L 0.54 L 1.9 L 1.8 L 1.9 L 1.8 L

Qty. ea. ea. 10-pk 10-pk ea. ea. 10-pk 10-pk

Price $ 282.00 282.00 214.72 214.72 332.00 332.00 322.08 322.08