Eco-Culture a New concept for Clonal Multiplication of Plants

By John Paily A clone is a duplicate of living system and its information. The normal process by which life sustains itself is through mixing of information from male and female. The traits and characteristic of the plants obtained from seed changes because of mixing of information. This is a serious disadvantage in planning a quantitative economy of agro industry. Humankind has always struggled to predict, plan and reap. A clone that over steps the mixing of the information is thus assumed a solution. Plants are known to be toti-potent. In other words, they have retained the capacity to develop complete plants from parts. It was noted from time immemorial that many plant part in appropriate condition, could produce a complete plants. This observation gave rise to techniques of rooting, grafting and such technique to get cloned plants. Developments in Plant science showed that the plant growth and its differentiation of root and shoot are controlled by ratio of hormones; auxins and cytokinins. This in turn led to experiments to manipulate plant parts in test tubes in the laboratory, in agar mediums, containing artificial nutrients and light. This research led to inducing and controlling the shoot and root developments and even creation of artificial embryos [embryoids] from plant parts in test tubes. This then paved way for a branch of biotechnology called Tissue Culture Industry. The industry called tissue culture and biotechnology

in general has failed to grow up to the potentials it proposed in spite of consuming huge resources in terms of research and developments. The reason are many We do not know why life mixes its information? Why plant are toti-potent? Why plants being toti-potent still prefers mixing of information? It is built on assumption that information is a static entity and it exist in Genes and DNA. It fails to comprehend spiritual, qualitative and dynamic reality of information In short the industry called bio-technology is not based on full understanding of life. Thus it is back firing.

The problem faced by the Tissue Culture industry 1] There is no single rule and method applicable to all plants. This makes it mandatory that one has to develop a method for every species. This involved huge research input into developing appropriate media, choosing or inventing appropriate hormones [chemical conditions], fixing physical conditions etc. My own experience of observing thousands of cultures in vitro and life in nature tells me that within the species the plants and animals have individuality. They are unique in space and time. They are the product of energy interaction of the system with energy of the surrounding, which is changing with time. This makes the control of genetic information further difficult. A process developed for one species and variety have tendency to fail when applied to another variety in the same species. Even the physico-chemical factors to which Mother Plant are exposed also plays an important role. This means a developing a stable technology that maintains the stability of information not only is a arduous task, but also is not compatible with life and its Natural expression. 3] Plants obtained by callus culture [where cells are dedifferentiated and differentiated to form embryoids, which then give rise to plants] showed higher diversity than the seed derived plants thus defeating the objective of

industry oriented research. It exposed the lack of our basic understanding of information and its expression in biological system. There is some stability in plants obtained from axillary bud multiplication. Commercial multiplication through axillary bud multiplication in plants has been achieved in banana, cardamom and many other plants. Here too reports exist of unexplained failure and uncertainty. This peaks life's information is dynamic and it is not quantified one. These problems are further amplified by the arduous task of acclimatizing the fragile plants grown in test tube in artificial sterile medium to natural conditions. At the practical level, it included educating and supporting the farmers to handle these delicate plants to its ultimate end. Many famous genetic engineering scientist like Dr. David Zusuki have openly come out opposing genetic engineering and biotechnology However, the research industry and concepts have survived by hiding the negative aspect over exaggerates and advertises to create a vortex to lure more investment to keep it alive. This is done against once own consciousness making it more and more fragile. Yet I must admit that within this unreal growth a space also develops for real growth, which from time to time emerges to support the system. The concept I speak here emerges from my back drop of tissue culture and biotechnology and a mind that always seeks simple and natural solution The Logic of Eco-Culture concept The tissue culture research and industry is built on the realization that the ratio of auxin /cytokinin [hormones] controls the growth and differentiation of plants. Auxin favoring root formation and cytokinins favoring shoot growth. A tissue culturist trying to clone plants tries to develop by trial and error a physico-chemical condition appropriate to the species he selected then uses a set of known chemicals hormones called cytokinins and auxins

[some of which are known carcinogens] to control the growth and differentiation. Observation of the plant system in nature tells us a fact that the plant produces its own hormones to cause the growth in response to nature and its cycle of energy change. Leafs seem to produce auxins and roots cytokinins -Two observations gave way to eco-culture 1] It was noted that most young branches with few leafs when kept in highly humid conditions has potentials to develop roots. 2] The axillary buds are induced when the climatic conditions change. They seemed to react to energy changes in nature. This opened up a physical means of energy management of environment to producing cloned plants by simply controlling the internal mechanism of the plants that produce the auxin and cytokinins. In the previous article "Seed that Changed My life" and I did speak "Physical vision of Life". This actually goes to support it, The Technique was experimented with cashew and coffee plants. The following are the broad steps involved in it 1] Rooted plants of cashew and coffee were grown in controlled environment to promote vegetative growth, when the plants are gown 4 to 10 steps. The apical meristem along with two leaves were removed and was transferred to another pot for rooting.

2] The decapitated plant is subjected to high energy state [light, high humidity and nutrients] to initiate the axillary buds 3] The plants were then shifted back to lesser light and over cast humid conditions; this promotes the growth of the axillary shoot. It is returned to optimal condition for growth. As the axillary shoot grows by two-step its apical meristems along with two leaves and a short stem [one-step] is removed and potted for rooting. The rooting conditions were achieved by increased moisture by covering the plant with a plastic bag see fig. 4] The mother plant is subjected to increased energy conditions [light and nutrients] to produce more axillary shoots.

Note - The substratum used for rooting was ordinary sand that was heated to reduce the risk of infection. Even without heating it gave fairly good results. Important point is that what we used here is the potentials of the green leaf to produce its own hormones to produce the root growth and then the roots to support the shoot growth to form complete plants. It was noted that when leaf axil tissue culture was attempted in laboratory conditions, the axil would show some initial growth then seizes to grow, clearly suggesting some factor specific to species and its growth is lacking in the media. The same experiment with part leaf attached to the explant showed better growth of shoots See Fig. Most interesting fact is that the same experiment conducted outside in glass house with natural light produced very good results. See Fig

These works were repeated for coffee plants with similar results.

Conclusion
In conclusion I have only two point to say 1] There is more to life than we think and understand in modern science. Modern biology is built by studying a dead body. Life is not genes or DNA, it is a spiritual entity, a space-time field and a universe by itself. What we deal as information [Gene and DNA] and body is something the spiritual entity builds in order to survive in this world. This means we can change traits and information of whole system by working on whole system much more successfully than breaking the system down to its parts. Einstein said " Any

intelligent fool can make things bigger, more complex, and more violent. It takes a touch of genius -- and a lot of courage -- to move in the opposite direction."
We need people like Fr. Gregor Mendel who became Father of modern genetics working from his Kitchen Garden than people working in high tech labs like Monsanto. My experience says that every old farmer is store house of living information gained by experience. We are actually losing them under modernization. We call Fr. Gregor Mendel the Father of Genetic yet we failed to understand the "ratios" 1:2:1. 2] Nature has simple answers to much our problems provided we break beyond our "self" and seek her. We then evolve from complexity to simplicity, from bondage to liberation. Beyond self free from "Plato's Chair" if we care to observe and meditate on a single life it can open the gate to secret of whole kingdom of life and beyond it, much the same mediating truly on single "word of God" can open the secret of whole scripture. suggested read - Seed that Changed My Life -

http://www.scribd.com/doc/162644145/Seed-That-Changed-My-Life