BIOGRAPHICAL SKETCH

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NAME POSITION TITLE

Igor A. Prudovsky Scientist II
eRA COMMONS USER NAME
IgorPrudovsky
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
DEGREE
INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY
(if applicable)
M.S. (Summa
Moscow University (Summa Cum Laude) 1975 Cellular Biology
Cum Laude)
Engelhardt Inst. Molecular Biology, Moscow Ph.D. 1979 Cellular & Molecular Biology
Engelhardt Inst. Molecular Biology, Moscow Dr. Biol. Sci 1991 Cellular & Molecular Biology

A. Positions and Honors

Positions and Employment
1975-1978 Postgraduate Student, Engelhardt Institute of Molecular Biology, Moscow, Russia
1978-1985 Junior Scientist (equiv. to Postdoctoral) Engelhardt Inst. of Molecular Biology, Moscow, Russia
1985-1992 Senior Scientist (-1990 equiv. to Assist. Professor; 1990- equiv. to Assoc. Professor) Engelhardt
Institute of Molecular Biology, Moscow, Russia
1992-1998 Leading Scientist (equiv. to Professor), Engelhardt Inst. of Molecular Biology, Moscow, Russia
1993-1995 Visiting Scientist, Holland Laboratory of American Red Cross, Rockville, MD
1998-Present Scientist II, Ctr. for Molecular Medicine, Maine Med. Ctr. Res. Inst., Maine Medical Center,
Scarborough, ME
1999-Present Director of So. Maine Confocal Microscopy Facility, Maine Med. Ctr. Res. Inst., Maine Med. Ctr.,
Scarborough, ME
1999-Present Member of the Graduate Faculty, Cooperative Graduate Program in Molecular Genetics and
Cell Biology, University of Maine, Orono, ME

B. Selected Peer-Reviewed Publications (selected from 80 peer-reviewed publications)

1. Prudovsky I, Gumeniuk RR, Yegorov Y E, Poletayev AI, Zelenin AV. “Immortalized phenotype and the
presence of active oncogenes correlate with the capacity of culture cells to induce reactivation of DNA
synthesis in macrophage nuclei in heterokaryons”. Cell Differ Dev 1989; 26:221-228.
2. Zelenin AV and Prudovsky IA. Regulation of DNA synthesis investigated on heterokaryons of dividing and
nondividing cells. Int Rev Cytol 1989; 117:179-213.
3. Prudovsky IA, Gumeniuk RR, Yegorov Ye E, Zelenin AV. Resident macrophages specifically inhibit DNA
synthesis in the nuclei of transformed cells in heterokaryons. Int J Cancer 1989; 44:1005-08.
4. Prudovsky IA and Tsong TY. Fusion of fibroblasts with differentiated and nondifferentiated leukaemia cells
resulting in blockage of DNA synthesis. Dev Biol 1991; 144:232-239.
5. Fedorov YV, Prudovsky I, Yegorov YE, Zelenin AV. The comparison of DNA synthesis inducibility by high
potassium treatment in various non-proliferating cells. Exper Cell Res 1993; 209:156-59.
6. Prudovsky I, Kosimov RB, Prasolo, VS, Pospelova TV, Sukharev SI, Zelenin AV. The control of DNA
replication in hybrids between neutrophiles and fibroblasts. Cell Prolif 1993; 26:221-33.
7. Prudovsky I, Savion N, Friesel R, Xu J, Hou J, McKeehan W, Maciag T. Intact and functional FGFR-1
trafficks near the nucleus in response to FGF-1. J Biol Chem 1994; 269:13720-24.
8. Garfinkel S, Hu X, Prudovsky I, McMahon G, Kapnik E, McDowell S, Maciag T. FGF-1-dependent
proliferative and migratory responses are impaired in senescent human umbilical vein endothelial cells and
correlate with the inability to signal tyrosine phosphorylation of fibroblast growth factor receptor-1
substrates. J Cell Biol 1996; 134:783-91.
 9. Prudovsky IA, Savion N, LaVallee TM, Maciag T. The nuclear trafficking of extracellular fibroblast growth
factor (FGF)-1 correlates with the perinuclear association of the FGF receptor-1α isoforms but not the FGF
receptor-1β isoforms. J Biol Chem 1996; 271:14198-205.
10. Yegorov Y, Akimov S, Zelenin A, Hass R, Prudovsky IA. Endogenous beta-galactosidase activity in
continuously non-proliferating cells. Exper Cell Res 1998; 243:207-11.
11. LaVallee T, Prudovsky I, McMahon G, Hu X, Maciag T. The activation of MAP kinase pathway by FGF-1
correlates with proliferation while activation of the Src pathway correlates with migration. J Cell Biol 1998;
141:1647-58.
12. Tarantini F, Micucci I, Bellum S, Landriscina M, Garfinkel S, Prudovsky I, Maciag T. The Precursor but not
the mature form of IL1α blocks the release of FGF1 in response to heat shock. J Biol Chem 2000;
276:5147-51.
13. Landriscina M, Prudovsky I, Mouta Carreira C, Tarantini F, Maciag T. Amlexanox reversibly inhibits cell
migration and proliferation and induces the Src-dependent disassembly of actin stress fibers in vitro. J Biol
Chem 2000; 275:32753-62.
14. Wong MKK, Prudovsky I, Vary C, Booth C, Liaw L, Mousa S, Small D, Maciag T. A non-transmembrane
form of Jagged-1 regulates the formation of matrix-dependent cord-like structures. Biochem Biophys Res
Comm 2000; 268:853-59.
15. Landriscina M, Bagalá C, Mandinova A, Soldi R, Micucci I, Bellum S, Prudovsky I, Maciag T. Copper
induces the assembly of a multiprotein aggregate implicated in the release of fibroblast growth factor 1 in
response to stress. J Biol Chem 2001; 276:25549-57.
16. Landriscina M, Soldi R, Bagalá C, Bellum S, Tarantini F, Prudovsky I, Maciag T. S100A13 participates in
the release of FGF1 in response to heat shock in vitro. J Biol Chem 2001; 276:22544-52.
17. Carreira CM, Landriscina M, Bellum S, Prudovsky I, Maciag T. The comparative release of FGF1 by
hypoxia and temperature stress. Growth Factors 2001; 18:277-85.
18. Lindner V, Booth C, Prudovsky I, Small D, Maciag T, Liaw, L. Members of the Jagged/Notch gene families
are expressed in injured arteries and regulate cell phenotype via alterations in cell matrix and cell-cell
interaction. Am J Pathol 2001; 159:875-83.
19. Landriscina M, Bagalá C, Mandinova A, Soldi R, Micucci I, Bellum S, Prudovsky I, Maciag T. Copper
induces the assembly of a multiprotein aggregate implicated in the release of fibroblast growth factor 1 in
response to stress. J Biol Chem 2001; 276:25549-57.
20. Lindner V, Booth C, Prudovsky I, Small D, Maciag T, Liaw L. Members of the Jagged/Notch gene families
are expressed in injured arteries and regulate cell phenotype via alterations in cell-matrix and cell-cell
interactions. Am J Pathol 2001; 159:875-83.
21. Small D, Kovalenko D, Kacer D, Liaw L, Landriscina M, Di Serio C, Prudovsky I, Maciag T. Soluble Jagged
1 represses the function of its transmembrane form to induce the formation of the Src-dependent chord-like
phenotype. J Biol Chem 2001; 276:32022-30.
22. Prudovsky I, Popov K, Akimov S, Serov S, Zelenin A, Meinhardt G, Baier P, Sohn C, Hass R. Antisense
CD11b integrin inhibits the development of a differentiated monocyte/macrophage phenotype in human
leukemia cells. Eur J Cell Biol 2002; 81: 36-42.
23. Prudovsky I, Bagalá C, Tarantini F, Mandinova A, Soldi R, Bellum S, Maciag T. The intracellular
translocation of the components of the FGFR1 release complex precedes their assembly prior to export. J
Cell Biol 2002; 158:201-08.
24. Small D, Kovalenko D, Soldi R, Mandinova A, Kolev V, Trifonova R, Bagalá C, Kacer D, Battelli C, Liaw L,
Prudovsky I, Maciag T. Notch activation suppresses fibroblast growth factor-dependent cellular
transformation. J Biol Chem 2003; 278:16405-13.
25. Mandinova A, Soldi R, Graziani I, Bagalá C, Bellum S, Landriscina, M., Tarantini, F., Prudovsky, I. and
Maciag, T. S100A13 mediates the copper-dependent stress-induced release of IL1α from both human
U937 and murine NIH 3T3 cells. J Cell Sci 2003; 116:2687-696.
26. Mandinov L, Mandinova A, Kyurkchiev S, Kyurkchiev D, Kehayov I, Kolev V, Soldi R, Bagalá C, deMuinck
ED, Lindner V, Post MJ, Simons M, Bellum S, Prudovsky I, Maciag T. Copper chelation represses the
vascular response to injury. Proc Natl Acad Sci USA 2003; 100:6700-05.
27. Bagalá C, Kolev V, Mandinova A, Soldi R, Mouta C, Graziani I, Prudovsky I, Maciag T. The alternative
translation of synaptotagmin 1 mediates the non-classical release of FGF1. Biochem Biophys Res
Commun, 2003; 310:1041-47.
28. Prudovsky I, Mandinova A, Soldi R, Bagalá C, Graziani I, Landriscina M, Tarantini F, Duarte M, Bellum S,
Doherty H, Maciag T. The non-classical export routes: IL1α and FGF1 point the way. J Cell Sci 2003;
116:4871-81.
29. Trifonova R, Small D, Kacer D, Kovalenko D, Kolev V, Mandinova A, Soldi R, Liaw L, Prudovsky I, Maciag
T. The non-transmembrane form of Delta1 but not of Jagged1 induces normal migratory behavior
accompanied by FGF receptor 1-dependent transformation. J Biol Chem Acc Publ 2004; 279:13285-88.
30. Andreeva V, Prudovsky I, Maciag T. Stimulation of quiescent cells by individual polypeptide growth factors
is limited to one cell cycle. Euro J Cell Biol 2004; 83:327-35.
31. Doyle A, Fick J, Himmelhaus M, Eck W, Graziani I, Prudovsky I, Grunze M, Maciag T, Neivandt D. Protein
deformation of lipid hybrid bilayer membranes studied by Sum Frequency Generation Vibrational
Spectroscopy (SFS). Langmuir 2004, 20:8961-65.
32. Sivaraja V, Kumar KS, Prudovsky I, Yu, C. Three-Dimensional Solution Structure of Mouse S100A13, a
Component of the Fibroblast Growth Factor 1 Non-Classical Release Pathway, Biochem Biophys Res
Comm 2005; 335:1140-48.
33. Kolev V, Kacer D, Trifonova R, Small D, Duarte M, Soldi R, Graziani I, Sideleva O, Larman B, Maciag T,
Prudovsky I. The Intracellular Domain of Notch Ligand Delta1 Induces Cell Growth Arrest, FEBS Letters,
2005; 579:5798-802.
34. Rajalingam D, Kumar TKS, Soldi R, Graziani I, Prudovsky I, Yu C. Molecular Mechanism of Inhibition of
Non-Classical FGF-1 Export. Biochemistry 2005; 44:15472-79.
35. Omoto A, Kawahito Y, Prudovsky I, Tubouchi Y, Kimura M, Ishino H, Wada M, Yoshida M, Yoshimura R,
Yoshikawa T, Sano H. Copper chelation with Tetrathiomolybdate suppresses adjuvant-induced arthritis and
inflammation-associated cachexia in rats. Arthritis Research and Therapy. Arthritis Research and Therapy
2006; 7:R1174-82.
36. Sivaraja V, Kumar STK, Rajalingam D, Graziani I, Prudovsky I, Yu C. Copper binding affinity of S100A13,
a key component of FGF1 non-classical copper-dependent release complex. Biophys J 2006;91:1832-43.
37. Graziani I, Bagala C. Duarte M, Soldi R, Kolev V, Tarantini F, Kumar TK, Doyle A, Neivandt D, Yu V, Maciag
T, Prudovsky I. Release of FGF1 and p40 synapttotagmin 1 correlates with their membrane destabilizing
ability. Biochem Biophys Res Commun 2006; 349:192-99.
36. Landriscina M, Scinzari G, Di Leonardo G, Quirino M, Cassano A, D'Argento E, Lauriola L, Scerrati M,
Prudovsky I, Barone C. S100A13, a new marker of angiogenesis in human astrocytic glyomas. J
Neurooncol 2006; 80:251-259.
37. Duarte M, Kolev V, Soldi R, Kirov A, Graziani I, Marta Oliveira S, Kacer D, Friesel R, Maciag T, Prudovsky I.
Thrombin induces rapid PAR1-mediated non-classical FGF1 release. Biochem Biophys Res Comm 2006;
350:604-609
38. Soldi, R., Mandinova, A., Venkataraman, K., Hla, T., Vadas, M., Pitson, S., Duarte, M., Graziani, I., Kolev,
V., Kacer, D., Kirov, A., Maciag, T. Prudovsky, I., "Sphingosine Kinase 1 is a critical component of the
copper-dependent FGF1 export pathway". Exper. Cell Res. 2007; 313: 3308-3318.
39. Rajalingam, D., Graziani, I., Prudovsky, I., Yu, C., Kumar, TK.., "Relevance of partically structured states in
the non-classical secretion of acidic fibroblast growth factor". Biochemistry, 2007; 46: 9225-9238..
40. Rajalingam, D., Kacer, D., Prudovsky, I., Thallapuranam, S.K.K.T., "Molecular cloning, overexpression and
characterization of interleukin 1 alpha". Biochem. Biophys. Res. Commun. 2007; 360: 604-608.
41. Di Serio, C., Pellerito, S., Duarte, M., Massi, D., Naldini, A., Cirino, G., Prudovsky, I., Santucci, M.,
Geppetti, P., Marchionni, N., Masotti, G., Tarantini, F. "Protease-activated receptor (PAR) 1 selective
antagonist SCH7997 inhibits cell proliferation and induces apoptosis, by a PAR-1 independent
mechanism". Basic and Clin. Pharmacology and Toxicology 2007; 101: 63-69.
42. Kathir, K, Ibrahim, K., Rajalingam, D., Prudovsky, I., Yu, C., Kumar TKS. "S100A13-lipid interactions role in
the non-classical release of the acidic fibroblast growth factor". Biochim. Biophys. Acta 2007; 1768: 3080-
3089.
43. Nikopoulos, G., Duarte, M., Kubu, C., Bellum, S., Friesel, R., Maciag, T., Prudovsky, I., Verdi, J., "Soluble
Jagged1 attenuates lateral inhibition, allowing for expansion of neural crest stem cells". Stem Cells 2007;
25: 3133-3142.
44. Prudovsky, I., Tarantini, F., Landriscina, M., Neivandt, D., Soldi, R., Kirov, A., Small, D., Kathir, KM,
Rajalingam D., and Kumar, TKS. "Secretion without Golgi". J Cellular Biochemistry 2008; 3: 1327-1343.
45. Leclair, RJ, Wang, Q, Benson, MA, Prudovsky, I, Lindner, V. "Intracellular localization of Cthrc1
characterizes differentiated smooth muscle cells". Arterioscler Thromb Vasc Biol. 2008; 28:1332-1338.
46. Di Serio, C, Doria, L, Pellerito, S, Prudovsky, I, Micucci, I, Massi , D, Landriscina, M, Marchionni, N,
Masotti,, G., M, Tarantini, F. "The release of fibroblast growth factor-1 from melanoma cells requires copper
ions and is mediated by phosphatidylinositol 3-kinase/Akt intracellular signaling pathway". Cancer Letters.
2008. Epub ahead of print.
47. Duarte, M., Kolev V., Kacer, D., Mouta-Bellum, C., Soldi, R., Graziani, I., Kirov, A., Friesel, R., Liaw, L.,
Small, D., Verdi, J., Maciag, T., Prudovsky, I. “Novel cross-talk between three cardiovascular regulators:
thrombin cleavage fragment of Jagged 1 induces FGF1 expression and release”. Molecular Bio0logy of the
Cell 2008, accepted.

C. Research Support
Ongoing Research Support

R01 HL 32348 Prudovsky (PI) -03/31/04 11/30/01-11/30/08 (no cost extension)

“Growth Factor Signaling and Traffick in Angiogenesis”
The major goal of this project is to define the mechanism of cross-talk between the FGF and Notch signaling
pathways.
Role: PI

Maine Cancer Foundation, 07/01/08-06/30/09, FGF-Mediated Tumor Growth Induced by Notch Signaling
Inhibition.
The major goal of this project is to assess the role of FGF release and signaling in the transformed phenotype
of cells with inhibited Notch signaling.
Role: PI

P20RR15555 Friesel (PI) 09/15/05-04/30/10

NIH/NCRR
COBRE in Vascular Biology
Project 4: "Mechanism of Non-classical Release of the Angiogenesis Regulator, Interleukin 1α"
09/15/05-04/30/10
The major goal of this project is to define the specific characteristics of the non-classical release of Interleukin
1 alpha that distinguish it from the export of FGF1.
Role: PI

Core B: Protein, Nucleic Acid Analysis and Cell Imaging Core Vary (PI) 09/15/05-04/30/10
The Aim of this Core is to provide resources and reagents for gene expression, DNA sequence and protein
structure analysis, and cell imaging capabilities.
Role: Co-Investigator

Completed Research Support

P2015555 Friesel (PI) 09/01/00-06/30/05
NIH/NCRR
COBRE in Angiogenesis
Project 1: “Cooperativity Between FGF1 and Jagged1 Signaling in Angiogenesis”
The goal or aim of this project was to understand the interplay between two major signaling systems regulating
angiogenesis: FGF/FGFR and Jagged/Notch.
Role:PI

R01 HL35627 Prudovsky (PI) -03/31/04 06/30/01-06/30/07

NIH/NHLBI
“FGF-1 Secretion and the Pathology of Angiogenesis”
The major goal of this project was to define proteins participating in FGF export.
Role: PI