Beruflich Dokumente
Kultur Dokumente
F.
Place the tubing with the ferrule and the 10-32 PEEK bolt into the 10-32 port and push the tubing through the bolt until it fits snugly against the bottom of the port. Making sure that there is no gap between the end of the tubing and the bottom of the port will ensure low dead volumes and minimal band broadening. This is important for maximum system performance.
G. For the best performance, end fittings should be mated to ports. Once a ferrule has been compressed inside a particular 10-32 port, it can be removed and reinstalled in the same port without sacrificing performance. If a liquid line needs to be routed to a new port, a new ferrule should be mounted on the end of the line and compressed in the new port to form a mated ferrule/port pair. This ensures the best sealing properties. CAUTION Tighten the 10-32 PEEK bolt by hand until it is fingertight. As the bolt is tightened further, the ferrule will compress tightly around the tubing. In the case of Tefzel tubing, the compression of the ferrule will also compress the semi-rigid Tefzel tubing. Overtightening the bolts can reduce the ID of Tefzel tubing until flow through the tubing is completely restricted. Severe overtightening of the bolt in all cases can damage the mating surfaces of the 10-32 port. Fingertight torquing of the liquid line bolts provides leak free operation up to 3,000 psi. A Tefzel transition liquid line with a PEEK 10-32 fitting on one end and a 1/4-28 ThermoFlare fitting on the other end can be used in applications with or without solvent where the tubing is not subject to pressures over 2,000 psi. The following Tefzel transition liquid lines can be obtained from DIONEX:
Description Tefzel liquid line, 0.007" ID x 12" long with one 10-32 and one 1/4-28 fitting Tefzel liquid line, 0.012" ID x 12" long with one 10-32 and one 1/4-28 fitting
To minimize dead volumes, reposition the 10-32 threaded fitting bolt (P/N 043275) and a double-cone ferrule (P/N 043276) on the line to make the length of the line between components in the eluent flow path as short as possible. If you already have a Tefzel liquid line with ThermoFlare fittings on both ends for use between components with 1/4-28 ThermoFlare end fittings, you may choose to cut off one of the 1/4-28 ThermoFlare end fittings with a razor blade and replace it with a 10-32 PEEK ferrule/bolt assembly. Since the Tefzel tubing OD is larger than 0.062", it will not easily slide into the PEEK ferrule. A short section of the Tefzel tubing should be stretched slightly to reduce its diameter (see Figure 2, "Making a Tefzel Liquid Line with a 10-32 Fitting").
Beginning with a Tefzel Liquid Line with two 1/4-28 ThermoFlare/bolt assemblies:
Step 1:
Step 2: Stretch the tubing only if it does not slip through the ferrule.
Step 3: Cut the tubing at the smallest diameter in the stretched region.
Step 4:
Slip a 10-32 PEEK bolt and ferrule on the stretched tubing and then pull them PAST the stretched section of tubing.
Step 5: Cut the tubing perpendicular to its axis and approximately 1/8" from ferrule. Remove ALL of the stretched tubing!
Tighten the 10-32 ferrule/bolt assembly fingertight ONLY. Overtightening will constrict the Tefzel tubing Figure 2 Making a Tefzel Liquid Line with a 10-32 Fitting
Document No. 031432-01 July 1998