LCQ Deca/Duo Hardware Troubleshooting

Ed Gonzalez Product Support

Presentation Topics
Overview of the LCQ Ion Optic System Tour of the LCQ Ion Optic System
Making Ions Transferring Ions Filtering Ions (Quadrupole Ion Trap) Detecting Ions

LCQ Maintenance LCQ Diagnostic Overview Front Panel LEDs Indications

Mass Spectrometer
Simplified Schematic

Sample In

Make Ions

Transfer Ions

Filter Ions

Detect Ions

Ion Optics System

Data Out

Choice in Making Ions

Polarity vs. Molecular Weight ESI
15,000 1,000 200,000

APCI
Molecular Weight

GC

TSP PBI

FAB

Non Polar

Polar

Simplified LCQ Schematic

Transfer Ions
Sheath Liquid ESI Needle Assembly Heated Capillary Sheath Gas Tube Lens Octapole Endcap Electron Multiplier

Detect Ions
Mechanical Pump Auxiliary Gas Turbo Pump Turbo Pump +15 kV Ring Conversion Dynode Electrode

Skimmer APCI Probe Assembly

Make Ions

Desolvate Ions

Filter Ions

LCQ Probes used in Making Ions

API-1 / ESI Probe (LCQ Classic) API-2 / ESI Probe (LCQ Deca / LCQ Duo) API-2 / Off Axis ESI Probe (Classic/Deca/Duo) APCI Probe (Same for API-1 and API-2)

ESI Ionization Process

Simplified Schematic

ESI Spray Cross Section

Gas Sheath 5kV

Nozzle
Needle Ion Plume Liquid Sheath

Aerosol Formation
Relative velocity between the sheath gas flow and the liquid flow causes a shearing effect on the emanating large droplet, and results in rapid droplet size reduction (spray)

Sheath Gas

Sample Tube

Heated Capillary

Field lines
Applied high voltage activates the coulombic explosion process

API-1 / ESI Probe Assembly

(LCQ Classic)

Cal Mix Tune Parameters

API-1 on LCQ Classic

Infusion Flow Rate (L/min.): 3-5 Spray Voltage (kV): Spray Current (A): Sheath Gas Flow Rate (arb): Aux. Gas Flow Rate (arb): Capillary Temperature: Probe Position : 4-6 0.1-0.75 30-60 0 200-250C

Pulled all the way back

LCQ Deca Tune Plus Window

ESI Source dialog and Status Panel

Troubleshooting the API1 Probe

Spray Current too high: Erratic Spray Voltage: Erratic spray: Contamination:
High Voltage (HV) shorting effects: Leaks within ESI Probe Problematic mixture of solvents/sample/etc. Shorted HV cable Spay current at maximum Bad HV supply Sample tube ESI needle Sheath gas flow Wipe spray shield around heated capillary Wipe out ESI probe flange Flush the ESI probe Clean ESI probe interior parts good maintenance (especially with the sample tube) use of divert valve

Overall solution for 90% of source related Problems:

API-1 / ESI Probe Cross Section

Sample Tube Elongation

ESI Needle
Sheath Liquid

ESI Needle Polyimide ESI Needle Fused Silica

Sample
Polyimide Sheath Liquid
Fused Silica

Elongation of polyimide occurs when specific solvents are adsorbed into the sample tube. The sample tube must be cut to ensure good spray.

ESI Needle

ESI Needle
Sheath Liquid

ESI Needle Polyimide ESI Needle Fused Silica

The sample tube must be cut square to ensure good spray.

Sample
Polyimide Sheath Liquid
Fused Silica

Best results can be achieved by making the sample tube flush with the ESI Needle.

ESI Needle

Sample Tube Elongation Resolution

ESI Needle
Sheath Liquid ESI Needle Polyimide ESI Needle
Fused Silica

Sample
Polyimide Sheath Liquid
Fused Silica

The polyimide can be flamed to avoid elongation; however, make sure the end of the fused silica is cut square.

ESI Needle

ESI Needle
Sheath Liquid ESI Needle Polyimide
Fused Silica

Sample
Polyimide Sheath Liquid
Fused Silica

Again, make sure the end of the fused silica is cut square. In this case, the fused silica is cut on an angle. This will produce poor spray.

ESI Needle

Divert Valve Configuration

Front Panel Injections
GROUNDED TRANSFER LINE FITTING FITTING HOLDER SAMPLE TUBE

L oa d De t e ct or

I nj e ct W ast e

TRANSFER LINE FROM DIVERT/INJECT VALVE

2

1 5
S HEATH G AS

SYRINGE PORT
4

6
A U X IL I A RY GAS SAMPLE HIG H V O LTA G E

TRANSFER LINE FROM LC PUMP

DIVERT/ INJECT VALVE SAMPLE LOOP ESI SOURCE

S HEATH L I Q U ID

WASTE LINE

Divert Valve Configuration

Normal Applications
TRANSFER LINE FITTING
L o a d D ete ctor Inje ct W aste

GROUNDED FITTING HOLDER SAMPLE TUBE

TRANSFER LINE FROM DIVERT/INJECT VALVE

2 3 4 5 1
SHEAT H GAS AUXILIARY SAMPLE HIGH GAS VOLT AGE SHEAT H LIQUID

TRANSFER LINE FROM LC PUMP

DIVERT/ INJECT VALVE

ESI SOURCE WASTE LINE

FROM LC PUMP FROM LC

2 1

PUMP

2 1

TO ION SOURCE

3 5

TO ION SOURCE

3 5

6
WASTE

6
WASTE

TO MS

TO WASTE

Xcalibur Instrument Setup

Divert Valve Dialog

API-2 / ESI Probe Assembly

(LCQ Deca / LCQ Duo)

API-2 / ESI Probe Positions

321

Probe Positions

API-2 / ESI Guidelines

Operational Parameters
Heated Capillary Temperature
Typical setting: 150 to 275 C Typical setting: 350 C

LC Flow Rate
Infusion or LC at flow rates of <10 L/min LC at flow rates from 50 to 100 L/min

Suggested Probe Column Position Size (1 to 4)

Capillary 2

Sheath Gas
Required Typical setting: 20 to 40 units Required Typical setting: 80+ units

Auxiliary Gas
Not required Typical setting: 0 units Not required, but might help depending on conditions Typical setting: 0 units

1 mm ID

LC at flow rates from 200 to 500 L/min

2 to 3 mm ID

Typical setting: 350 C

Required

Not required, but usually helps to reduce solvent Typical setting: background ions 80+ units Typical setting: 20 units Required Typical setting: 80 to 100 units Required Typical setting: 20 units

LC at flow rates from 0.5 (probe position 3) to 1 mL/min (probe position 4)

4.6 mm ID

3-4

Typical setting: 350 C

API-2 / ESI Probe

Exploded Pictorial

Cal Mix Tune Parameters

API-2 on LCQ Deca / LCQ Duo

Infusion Flow Rate (L/min.): 3-5 Spray Voltage (kV): Spray Current (A): Sheath Gas Flow Rate (arb): Aux. Gas Flow Rate (arb): Capillary Temperature: Probe Position : 4-6 0.1-0.75 20-40 0 200-250C 2

LCQ Deca Tune Plus Window

(ESI Source dialog and Status Panel)

Troubleshooting the API2 Probe

Contamination: Erratic spray: Spray Current too high: Erratic Spray Voltage:
Wipe spray shield around heated capillary Wipe out ESI probe flange Flush the ESI probe Sample tube Sheath gas flow

Problematic mixture of solvents/sample/etc.

Spray current at maximum

Overall solution for 90% of source related Problems:

Good maintenance (especially with the sample tube) Use of divert valve

API-2 / ESI Probe Assembly

Cross Section

API-2 / Off Axis ESI Probe

(LCQ Deca / LCQ Duo / LCQ Classic)

API-2 / Off Axis ESI

Probe Geometry
ESI probe on a 25 degree slide

Ion flow

Heated Capillary

Tangential Movement

matrix flow

API-2 / Off Axis ESI Guidelines

Operational Parameters
Probe Position (1 to 7)
4

LC Flow Rates

Slide Plate Position

Top

Heated Capillary Temperature

Typical setting: 250 C

Sheath Gas
Required Typical setting: 10 to 30 units Required Typical setting: 80+ units Required Typical setting: 80+ units

Auxiliary Gas
Not required Typical setting: 0 units Required Typical setting: 10 to 20 units Required Typical setting: 10 to 20 units

Infusion or LC at flow rates of <10 L/min

LC at flow rates from 50 to 100 L/min

Top

Typical setting: 350 C

LC at flow rates of >200 L/min

Top

Typical setting: 350 C

APCI Ionization Process

LCQ Classic APCI Probe

LCQ Deca / LCQ Duo APCI Probe

Typical APCI Tune Parameters

Conditions: Reserpine at 1ml/min

Vaporizer Temp (C): Discharge Current (A): Discharge Voltage (kV): Sheath Gas Flow Rate (arb): Aux. Gas Flow Rate (arb): Capillary Temp (C): Capillary Voltage (V): Tube Lens Offset (V):

400-550 (600 max.) 5 (10A max.) 4-6kV (read back) 50-80 0-20 125-250 10-40 30-60 50L/min. - 2mL/min.

Flow Rate capability:

LCQ Deca Tune Plus Window

APCI Source dialog and Status Panel

Troubleshooting the APCI Probe

Spray voltage erratic:

Spray current should not exceed 20A Once 20A level is reached, the spray voltage will be lowered to compensate for the 20A threshold

Spray current too high: Contamination: Lack of sensitivity:

Problematic mixture of solvents/sample/etc. Bake out the probe for 10-60 minutes at 50C above desired Vaporizer Temperature Make sure the corona needle is seated and is not deformed

API-1 / API-2 APCI Probe

Cross Section

Ion Transfer / Desolvation Process

Overview
+ + + + +

Solvent/Buffer

+ ++ + +

ESI Nozzle
( 8 kV)

Heated Capillary

Ion Stream

LCQ API Stack Gas Flow

Tube lens skimmer

1 Torr

Peek Holder

10-3 Torr

LCQ API Stack Assembly

Cross Section

Capillary must be flush with the Tube lens and Skimmer Mount. Kalrez OO- Ring

Manifold

Manifold
Peek Bushing Capillary Sleeve

Heated Capillary Specifications:

Heater Resistance: Platinum Sensor: 14 110

Cal Mix Tune Parameters

LCQ API Stack

Capillary Temp (C)*: Capillary Voltage (V): Tube Lens Offset (V):

200-250 10-40 30-60

* Heated Capillary I.D.: LCQ Classic / LCQ Duo: LCQ Deca: 400m 500m

LCQ Deca Tune Plus Window

Vacuum dialog and Status Panel

Troubleshooting the API Stack

Loss of Sensitivity: Clean skimmer and tube lens Low Convectron Gauge Pressure: Heated capillary plugged Cal Mix Contamination: Clean API probe and heated capillary area Spiky noise: Bent capillary tip or dirty heated capillary Constant Background: Possible heated capillary contamination Overall solution for 90% of API Stack related Problems:
Good maintenance: Clean/Rinse the heated capillary region and spray shield daily. Use of the divert valve Use of Spray Cap and Orthogonal Sampling Adapter

Caution:
Periodically empty the waste bottle to avoid potential back streaming of waste solvent into the source region.

Orthogonal Spray Adapter

Configuration
Orthogonal ion flow Heated capillary ESI Probe

Buffer deposition Focusing ring Liquid drains

Orthogonal Spray Adapter Guidelines

Operational Parameters

LC Flow Rate
Infusion or LC at flow rates of <10 L/min LC at flow rates from 50 to 200 L/min

Suggested Probe Heated Column Position Capillary Sheath Gas Auxiliary Gas Size (2 to 4) Temperature
Capillary 3 Typical setting: 300 C Typical setting: 350 C Required Typical setting: 10 to 30 units Required Typical setting: 80 units Not required Typical setting: 0 units Required Typical setting: 10 to 20 units

1 to 2 mm ID

Spray Cap
Configuration
Spray Shield

O-Ring

ESI Probe

Capillary Sleeve

Heated capillary

Spray Cap

Peek Bushing

Reduces Cal Mix contamination

Spray Shield

Spiky Noise Characteristics

Single Noise Spike
330.5 S#: 4 RT: 0.27 AV: 1 T: + p Full ms 100 90 80 70 e c n a 60 d n u b A 50 e v i t a l e R 40 30 20 10 326.6 0 326 327 327.6 328.0 328.6 329.0 328 329 330 330.9 331 m/z 331.8 332 333 334.3 334 334.6 335 NL: 2185325

335.6

Spiky Noise Characteristics

Particle Noise Spectrum of Cal mix
S#: 4 RT: 0.27 AV: 1 T: + p Full ms 100 90 80 1322.1 70 1222.1 e c n a 60 d n u b A 50 e v i t a l e R 40 30 20 10 0 200 400 600 800 1000 m/z 1200 1400 1600 1800 195.2 262.7 413.3 553.7 812.2 330.5 536.3 308.5 872.7 690.9 1022.3 959.6 1721.9 NL: 7692800 1421.9 1522.0 1621.8

Spiky Noise at 10 uscans

524.4

1122.1 1821.8

Heated Capillary Cross Section

Bent Capillary Tip
With time, compound will neutralize out on the skimmer. This will spot will eventually need to be cleaned; otherwise, field affects can reduce sensitivity.

+ + + ++ + + +

++ + + + + + + + +

Heated Capillary
Bent Capillary Tip

Tube Lens

Skimmer

Avoid bending the tip of the heated capillary. The tip of the capillary must remain off axis to the skimmer; otherwise, spiky noise or reduced sensitivity can occur. The distance between the end of the capillary and the skimmer opening must remain fixed.
Fixed distance

Ion Transfer Overview

Deca Ion Optic System

Ions will be trapped in stable trajectories

Fundamental RF on Ring

Ion Stream from skimmer Transfer Array

Ion Trap

Ion Optics
Gating Ions into the Ion Trap

Octapole 1 Offset: LCQ / LCQ Duo Quadrupole 1 Offset: LCQ Deca

Multipole RF

Octapole 2 Offset: LCQ / LCQ Duo / LCQ Deca

LCQ Deca Tune Plus Window

Ion Optics dialog and Status Panel

Cal Mix Tune Parameters

LCQ Ion Optics
Octapole 1 Offset (V) for Classic/Duo: Octapole 1 Offset (V) for Deca: Lens Voltage (v): Octapole 2 Offset (V) for Classic / Duo: Octapole 2 Offset (V) for Deca: Octapole RF Amplitude (V p-p): Entrance Lens (V) for the Deca only: -1 to -5 -4 to -9 -16 to -50 -5.5 to -10 -7 to -15 400 -35 to -60 V

Troubleshooting:
Loss of Sensitivity: Octapole Diagnostic errors: * Clean octapoles(multipoles) and lens * Tune multipole RF prior to running diagnostics.
* Multipole RF tune now performed in calibration for all LCQs run with Xcalibur.

LCQ Classic Optics

Typical Operating Pressures
1.7x10-3 torr 760 torr 1.0 torr 2.0 x10-5 torr (1.0x10-5 torr He)

3.5x10-3 torr He

30 m3/hr 100 L/sec

220 L/sec

Typical Operating Pressures/Comparison to LCQ Classic

760 torr 1.0 torr 1.7x10-3 torr 2.0 x10-5 torr (1.0x10-5 torr He)

LCQ Duo Optics

3.5x10-3 torr He

30 m3/hr 100 L/sec

220 L/sec

Typical Operating Pressures/Comparison to LCQ Classic

760 torr 1.3 torr 1.7x10-3 torr 2.0 x10-5 torr (1.0x10-5 torr He)

LCQ Deca Optics

3.5x10-3 torr He

60 m3/hr 100 L/sec

220 L/sec

Potential Energy Diagram

LCQ and LCQ Duo

4000 50

Potential

20 0

0 -3 -20 -23 -40 -27 -7 -10 -30 -15000

Source CID = 20%

Potential Energy Diagram

LCQ Deca

4000 50

Potential

20 0

0 -5 -20 -50 -15000 -7 -10

RF Tune Diagnostic Dialog

LCQ Deca

Tune Multipole RF
LCQ Deca

Multipole RF Tune
in Calibration Process

Multipole RF Tune verification performed prior to calibration.

LCQ Deca Ion Optics

Specifics Features
New Endcap Electrodes New Entrance Lens

Square Quadrupole

New Inter-Octapole Lens

Split Multipole

Deca / Duo Configurations

Split Square Quadrupole LCQ Deca

Split Octopole LCQ Duo

Deflection - set to +132 Deflection - set to -132

Transmit - both set to Offset Value

Effect of Split Multipole

100 90 80 Relative Abundance 70 60 50 40 30 20 10 0 200 300 400 500 m/z 600 700 800 900

No Split Multipole

MRFA m/z 524

(with isotopes)

100 90 80 Relative Abundance 70 60 50 40 30 20 10 0 200 300 400 500

With Split Multipole

MRFA m/z 524

(with isotopes)

600

700

800

900

LCQ Deca Noise Reduction

Split DC on Scan out -DC +DC Pulsed lens

DECA

No Split LCQ

Overview of Ion Separation

Quadrupole Ion Trap

Ion Stream from Transfer Array

Ion Trap
Fundamental (Ring Electrode) and Resonance Ejection (End Caps) RF potential are ramped to sequentially eject ions from the Ion Trap

Mass Selective Instability

Simplified Overview
az
0.2 0.1
0.2

qz= k v
z
0.3 1.0 0 0.8 0.1 0.7 0.6 0.2 0.4 0.5 0.3 0.4 0.5 0.6

(m/z)

0 - 0.1 - 0.2 - 0.3 - 0.4 - 0.5 - 0.6 - 0.7 0.2 0.4 0.6 0.8 1.0

r
0.7 0.8 0.9 1.0

q z-edge = 0.908
1.2 1.4 1.6

qz

Ion Trap Mass Analyzer

Filter Ions

Quadrupole Ion Trap

Operational Parameters
Main RF: (760kHz) Resonance Ejection RF: (frequency Varies) Waveform RF: (Arbitrary) Trap Offset: Exit Lens: Helium gas consumption: vacuum 16.2 kV p-p max. 80 V p-p max. 160 V p-p max. 10 V fixed at Ground potential 1 cc/min. under

LCQ Deca Tune Plus Window

Injection Control dialog and Status Panel

Troubleshooting the Ion Trap

Loss of Sensitivity: Clean End caps and ring electrode High Mass Noise: Clean spacer rings and endcaps. Lack of sensitivity/resolution/mass stability:
Lack of helium or Air leak

Notes:
Full scan target:
-Set to 5X10e7 for best sensitivity in the positive ion mode. -For best mass stability results, set to 2X10e7. -Set (2-3) times less in the negative ion mode. A typical value of 1X10e7 should be used. Positive/Negative switching: -Xcalibur allows for separate tune files. Pos/Neg scan segments can be used. ZoomScan target: -Set to (1-3)X10e6 for compounds with multiple charge. -Singly charged compounds will exhibit a slightly higher target. -For calibration with Cal Mix, set to 1X10e7.

Tune RF Frequency
LCQ Deca

Measure RF Frequency Detected RF RF Frequency Well Standing Wave Ratio Switch Status

Tune RF Modulation
LCQ Deca
Standing Wave Ratio Switch Status Detected RF Highest Mass
RF Modulation Upper and Lower Ranges

RF Modulation

High Mass Noise

S#: 34 RT: 1.11 AV: 1 T: + p ms 100 90 80 e c n a d n u b A e v i t a l e R 70 60 50 40 30 20 10 0 200 400 600 800 1000 m/z 1200 1400 1600 951.5 1196.3 901.9 1504.0 1229.1 1336.0 1641.4 1628.6 17 NL: 10610

High Mass noise due to a RF electrical discharge from inside the manifold.

Affects of Helium on Spectra

Helium flowing into trap
S#:1 RT:0.00 AV:1 SM:7G NL: 2.50E7 T: + p Full ms 100 Relative Abundance 80 60 40 525.3 20 0 514 516 518 520 522 m/z 524 526 528 524.3 S#:23-32 RT:0.71-1.00 AV:10 SM:7G NL: 5.61E7 T: + p Full ms 100 Relative Abundance 80 60 524.26 195.15 40 1122.21 1022.09 500 1000 m/z 1500 2000 1921.88 20 0 1322.06 1721.89 1222.14 1821.95 1522.04 1621.97

Helium shut off and not flowing into trap

S#:1 RT:0.02 AV:1 SM:7G NL: 9.70E6 T: + p Full ms 100 Relative Abundance 80 521.8 521.2 520.7 523.9 60 40 20 0 514 516 518 520 522 m/z 524 526 528 522.6 523.0 S#:23-32 RT:0.39-0.54 AV:10 SM:7G NL: 2.80E7 T: + p Full ms 100 Relative Abundance 80 1320.95 1220.75 523.01 1120.90 192.17 500 1000 m/z 1500 2000 1919.96 60 40 20 0 1620.79 1520.26 1720.44

Ion Detection

Electron Multiplier
Detection System
2-Particles enter the multiplier

Two particles formed when an ion ejected from the Ion Trap hits the dynode. Dynode particles enter the multiplier. Each particle hits the surface of the multiplier resulting in the ejection of two more particles.
Cathode Applied High Voltage

The cascading effect of this process will produce a charge on the anode cup. This charge represents the signal produced by the ion.

Anode cup

Signal to Data system.

LCQ Deca Tune Plus Window

Ion Detection System dialog and Status Panel

Detection System Parameters

Dynode Voltage: Electron Multiplier: 15kV (fixed) 2500 Volts max.

Note:
Multiplier voltage is a calibrated parameter. For best results, the multiplier should not be set manually.

Troubleshooting:
No peaks on Classic:
Check multiplier voltage Switch dynode polarity Check for ions

No peaks on Deca/Duo: Check Multiplier and Dynode Voltages. Noisy spectra with heated capillary capped off:
Potential Dynode noise Clean Dynode Cup

Dynode Noise
Dynode noise occurs over time. A noisy baseline with the heated capillary capped off is the symptom. This is due to an accumulation of material that can build up in the dynode cup over time. The cup should be cleaned when this occurs.

Life Time of the Electron Multiplier

Voltage

Time
The voltage needed to produce gain on the multiplier should show a linear increase with time.

LCQ Recommended Maintenance Schedule

Daily:

Morning Recommendations:

Check the convectron and ion gauge pressures. Make sure the vacuum system is operational. Check the fused silica sample tube. Make sure the fused silica has not elongated. Remove the septum cap. Check the convectron and ion gauges pressure again. Make sure the vacuum pressures are still OK. Start your analysis.

LCQ Recommended Maintenance Schedule

Daily:

Evening Recommendations:

Put the system in the stand-by mode. Turn off the Ion Gauge and rinse the Heated Capillary with methanol or an appropriate solvent. When finished, turn Ion Gauge back on. Cap off the heated capillary with the septum cap. Secure the API probe to the API stack. Make sure the solvent waste bottle is empty. Open the mechanical pump ballast for roughly 0.5 hour. While the pump is ballasting, maintain (move, delete, and/or copy) files at the LCQ computer. If bottled nitrogen gas is being used, check the nitrogen gas. After 0.5 hour, close the ballast valve.

LCQ Recommended Maintenance Schedule

Weekly:

For normal operation:

Check the mech. pump oil level. Fill the mech. pump as needed. If through put is high (running 24 hours a day), schedule one day a week to: - Change the mech. pump oil.
- Clean the skimmer and tube lens.

LCQ Recommended Maintenance Schedule

Monthly: Every Month:
Check the helium gas tank pressure. Replace as needed. Check the nitrogen gas tank (Dewer) pressure. Replace as needed. Nitrogen gas consumption:
Typical: 3-6 L/min Worse case scenario associated with choice of Nitrogen Generator: 15L/min (28SCFH at 100psi and 99% purity) for LCQ Classic 30L/min (56SCFH at 100psi and 99% purity) for Duo / Deca

Check the LCQ calibration. Check the air filter. Clean if necessary. Possibly, change the mech. pump oil.

LCQ Recommended Maintenance Schedule

Every 3 months:
Change the mech. pump oil.

6 months to a year:
Change the turbo pump oil.

Notes
- The API stack and analyzer should be clean as needed. - If the sensitivity starts to drop off and can not be restored, clean the API stack (and analyzer) as needed.

Overview of Diagnostics
Tests and Tools provided:
Static Tests Dynamic Tests Diagnostic Tools Research Tools

Limitations:
Provides status of electronics components Does not provide readbacks from the actual source element Can not detect a broken or loose connection.

Prior to running the dynamic diagnostics:

Tune all RF components (Octapole/Multipole and Main RF). Make sure the system in the ON mode. Make sure the API Probe is bolted to the API stack. This allows the high voltage to be activated.

Run All Diagnostics

Dynamic and Static Tests

Static Results from Status Test

+15 V - Pass +150 V - Pass +205 V - Pass +24 V - Pass +28 V - Pass +35 V - Pass +36 V - Pass +5 V - Pass -15 V - Pass -150 V - Pass -205 V - Pass -28 V - Pass 8 kV PS voltage - Pass Ambient temp. - Pass Analyzer temp. - Pass Aux gas flow - Pass Capillary temp. - Pass Capillary voltage - Pass Convectron - Pass Detected RF - Pass Dynode voltage - Pass Entrance lens - Pass Intermultipole lens - Pass Ion gauge - Pass Main RF DAC - Pass Multiplier setting - Pass Multiplier voltage - Pass Multipole 1 offset - Pass Multipole 2 offset - Pass Multipole RF mod. - Pass Multipole det. RF - Pass Multipole RF amp. out - Pass RF amp. output - Pass RF det. temp. - Pass RF gen. temp. - Pass RF modulation - Pass Sheath gas flow - Pass Trap DC Offset - Pass Tube/gate lens - Pass

Dynamic Results
RF Test
Starting All Diagnostics Scan Tests 16:18:30: 16:18:35: 16:18:37: 16:18:37: 16:18:42: 16:18:44: 16:18:44: 16:18:48: 16:18:50: 16:18:50: 16:18:55: 16:18:57: 16:18:57: 16:19:02: 16:19:04: Start scan readback test on device Auxiliary amplitude (V) -- 0 to 83.2 Scan readback test ended Result: PASSED Start scan readback test on device Main RF DAC (16-bit) -- 0 to 65535 Scan readback test ended Result: PASSED Start scan readback test on device Vernier det. RF amp. (V) -- 0 to 65535 Scan readback test ended Result: PASSED Start scan readback test on device Vernier RF DAC (16-bit) -- 0 to 65535 Scan readback test ended Result: PASSED Start scan readback test on device Multipole RF DAC (V) -- 0 to 1000 Scan readback test ended Result: PASSED

Dynamic Test
Graphical Output

Dynamic Results
Lenses Test
Starting All Diagnostics Scan Tests 16:19:04: 16:19:09: 16:19:11: 16:19:11: 16:19:16: 16:19:17: 16:19:17: 16:19:22: 16:19:24: 16:19:24: 16:19:29: 16:19:31: Start scan readback test on device Multipole 1 offset (V) -- -132 to 132 Scan readback test ended Result: PASSED Start scan readback test on device Multipole 2 offset (V) -- -132 to 132 Scan readback test ended Result: PASSED Start scan readback test on device Multipole lens (V) -- -132 to 132 Scan readback test ended Result: PASSED Start scan readback test on device Multipole det. RF amp. (Vp-p) -- 0 to 1000 Scan readback test ended Result: PASSED

Dynamic Results
Ion Detection Test
Starting All Diagnostics Scan Tests 16:19:31: 16:19:36: 16:19:38: 16:19:38: 16:19:43: 16:19:45: 16:19:45: 16:19:58: 16:20:00: Start scan readback test on device Trap Offset (V) -- -132 to 132 Scan readback test ended Result: PASSED Start scan readback test on device Tube gate (V) -- -200 to 198.01 Scan readback test ended Result: PASSED Start scan readback test on device Multiplier (V) -- 0 to -2200 Scan readback test ended Result: PASSED

Dynamic Results
API Source Test
Starting All Diagnostics Scan Tests 16:20:00: 16:21:35: 16:21:37: 16:21:37: 16:23:20: 16:23:22: 16:23:22: 16:23:27: 16:23:28: 16:23:28: Start scan readback test on device Auxiliary gas flow (arb) -- 0 to 60 Scan readback test ended Result: PASSED Start scan readback test on device Sheath gas flow (arb) -- 20 to 100 Scan readback test ended Result: PASSED Start scan readback test on device Capillary Voltage (V) -- -132 to 132 Scan readback test ended Result: PASSED Final result: PASSED

Power Supplies
Static Diagnostic

API and Temperature

Static Diagnostic

Static Diagnostic

Lenses

Static Diagnostic

RF-1

Static Diagnostic

RF-2

Calibration
Diagnostic Tool

Toggles/Detector
Diagnostic Tool

Instrument Settings
Diagnostic Tool

Plotting Conversion Dynode Voltage

Graphs

Plot Tube Lens Calibration

Graphs

Triggers

Research Tool

Front Panel LEDs

Classic/Deca

Front Panel LEDs

Definition of Classic/Deca
Power: Indication of the digital power
Should be green unless the power to the LCQ is off or there has been a failure.

Vacuum: Indicates that the vacuum is OK.

(Convectron gauge, ion gauge, and external switch are all in the correct state.) Should be green. If any one of the inter-locks is not logically correct, the LED will be off.

Communication:

Indicates communication between the on-board AT CPU and the NT

computer. Will be green if the two computers are communicating. Will be yellow if the on-board AT is active but not communicating with the NT computer. Will be off if the LCQ is off or if a failure has occurred.

System: Indicates the status of the LCQ.

Will be green if the LCQ is in the On mode. High voltage is applied. Will be yellow if the LCQ is in the standby mode. High voltages are off. Will be off if the LCQ is in the Off mode. Most of the power supplies are off.

Scan: Indicates that the LCQ is in the On mode and Scanning.

Will be blue and flashing when the LCQ is collecting data. Will be off if the LCQ is either in the On or Standby mode. Also will be off if the LCQ is off.

Front Panel LEDs

LCQ Duo

LCQ Duo Front Panel LEDs

Definition
Power: Indication of the digital power
Should be green unless the power to the LCQ is off or there has been a failure. Will flash yellow indicating a Warning condition for the on-board CPU temperature . Will be solid yellow indicating a Fatal condition. The MS will be held in a Reset mode until the temperature problem has been resolved.

Vacuum: Indicates that the vacuum is OK.

(Convectron gauge, ion gauge, and external switch are all in the correct state.) Should be green. If any one of the inter-locks is not logically correct, the LED will be off.

Communication: Indicates communication between the LCQ AT CPU and the NT computer.
Will be green if the two computers are communicating. Will be yellow if the on-board AT is active but not communicating with the NT computer. Will be off if the LCQ is off or if a failure has occurred.

LCQ Duo Front Panel LEDs

Definition
System: Indicates the status of the LCQ.
Will be green if the LCQ is in the On mode. High voltage is applied. Will be yellow if the LCQ is in the standby mode. High voltages are off. Will be off if the LCQ is in the Off mode. Most of the power supplies are off.

Scan: Indicates that the LCQ is in the On mode and Scanning.

Will be blue and flashing when the LCQ is collecting data. Will be off if the LCQ is either in the On or Standby mode. Also will be off if the LCQ is off.

Syringe Pump: Indicates the status of the syringe pump.

Will be green if the syringe pump on. Will be yellow when the pump has reached it end of travel.