European Cells and Materials Vol. 26. Suppl.

6, 2013 (page 60)

ISSN 1473-2262

Study of the solid-liquid-solid mechanism through of the growth of nanowires as neuroprotection and excitotoxity model in vitro
JJ de S. Melo1, LF Gomes1, FHS Sales1, RSB Melo2
1

Maranho Federal Institute, So Luis, MA, Brazil. 2 Federal University of Maranho, So Luis, MA, Brazil

INTRODUCTION: The solid-liquid-solid (SLS) model is growth mechanism of nanowires having solid material as catalyst [1]. In this work, the growth nanowires was investigated as effect SLS mechanism caused by dewetting transition phenomenon, where gold thin film on silicon substrate were used as study samples in order to analyze the influence of the catalytic particle gold in the viability and cellular adhesion in vitro of silicon surfaces coated with SiO2 nanowires. METHODS: Gold thin films are vacuum thermally evaporated over (100) Si substrates precleaned in HF solution for oxide removal, and submitted to a thermal treatment in Ar atmosphere in the sequence. The nanostructures are characterized via SEM/EDS and TEM images. The formation of the nanowires is observed at a heating temperature around 1050 C for at least 30 minutes, with a large range of diameter (~10 nm to ~1 m) and length sizes (to ~100 m). How the metal layer deposited on the outside surface of balance "hydrophobic" semiconductor, when heated, and there are craters formed (which grow along the intake Si atomic planes with Miller indices h = 1, k = 1 l = 1) geometry triangle evolves into hexagon, similar to what occurs in the anisotropic corrosion of Si (100). RESULTS: It was observed that the SLS mechanism occurs when the surface layer is submitted to thermal treatment high temperatures, but near the melting point of the solid material which constitutes it, resulting of square shaped holes, on gold surface, revealing the substrate surface, instead of isolated islands of Au [Fig 1]. The islands are not compact, featuring intricacies with straight edges, indicating the crystallization of Au along [111], as evidenced by X-ray diffraction. The Fig.2 shows that on samples treated at 1050 C was observed the formation of nanowires [2].

Fig 1: SEM images of the evolution of SLS mechanism on the film 50 nm gold deposited on Si after annealing at a temperature of 1050 C under argon atmosphere: a)30 b)60 c)90 d)120 minutes.

Fig 2: a) SEM image of nanowires with lengths exceeding 2m and containing high number of nanospheres. b) TEM images of gold particles within the nanowire. DISCUSSION & CONCLUSIONS: These nanowires can be used as a model system to study effects such as excitotoxicity or as study model of neuroprotection, in vitro, due the parameters its crystalline structure similarities to real systems. In this study we used the test by direct contact. The release of the toxic material can damage the cells or reduce the rate of cell growth in culture [3]. REFERENCES: 1E. Jiran and C.V. Thompson (1992) Thin Solid Films 208:23. 2D.P. Yu; Y.J. Xing; Q.L. Hang; H.F. Yan, J. Xu; Z.H. Xi; S.Q. Feng (2001). Physic E 9:305-309. 3Robinson J.T, Jorgolli M, Shalek A.K, Yoon M.H, Gertner R.S, Park H. (2012). Nat Nanotechnol. 10,7(3):180-4 ACKNOWLEDGEMENTS: The authors would acknowledge FAPEMA, CAPES and CNPq for financial support. http://www.ecmjournal.org