Dream team

Easier to digest; Introduction and plan

Group rules and roles

Rules
Roles: Aidan: Liz: Jess: Jay:

Experiment 1 quick description and guaiacol safety

observing the effects of different conditions such as pH, temperature concentration etc; on the rate of catabolic breakdown of peroxide by the enzyme peroxidase from turnips. RH2 + H2O2 -----------> R+ 2 H2O reducing agent = guaiacol

guaiacol Analysis: guaiacol is a colourless solution in it's normal state, but changes colour when it is oxidised. colour can measured using a spectrophotometer. Safety: eyes; guaiacol is an irritant and will cause redness and pain. [wear goggles] skin; causes irritation to skin. [ensure sleeves are completely down, wear gloves and wear suitable shoes] ingestion; Guaiacol is harmful if ingested. [do not drink, make sure to not touch your mouth after handling guaiacol and wash hands if necessary] inhalation; can be dangerous if inhaled. [presumably use a fume cupboard]
references: http://www.ch.ntu.edu.tw/~genchem99/msds/exp23/guaiacol.pdf http://www.sciencelab.com/msds.php?msdsId=9924208

guaiacol

H2O2 safety

Much like guaiacol Hydrogen peroxide is an irritant, so protective eyegear, handgear and clothing must be warn, and care must be taken not to ingest or spill during handling.

Experiment 1 method (finding the enzyme baseline)

Equipment required: fill 3 test tubes with the reagents shown above and label them, these will be Pipettes (10ml & 5ml& stock reagents, place them in a test tube rack. Fill a small beaker with distilled 1ml ) water, and then label a fourth test tube reaction mix. Pipette tips (10ml & 5ml& 1ml ) label 3 test tubes 1 2 and 3 and and fill them with the reagents shown in the 7 test tubes table in the handout. 1 small beaker A test tube rack transfer some solution from tube 1 into a cuvette and zero the bottle of distilled water spectrophotometer. ( we use a reagent blank as, although the reagent may cuvettes look clear it may be slightly tinted and will therefore have a different absorption to clear water, i.e. it prevents zero error of the spectrophotometer. reagents required: mix tubes 2 and 3 to make the sample mixture and quickly add some to a clean H202 0.1% concentration (3mls) cuvette this will be t = 0 then record the absorption at 500 nm every 20 seconds until 2 minutes is reached. guaiacol (3mls) turnip extract (5mls) then plot a graph of absorption against time. Absorption on the Y axis and time on the X

Results table and graph

Does the amount of enzyme influence reaction rate?

Prediction:

Procedure: Follow the procedure for experiment 1, but this time change the turnip extract concentration and measure the effects. (double and half it)

reference tables in handout

Our independently planned experiment. Does _____________ influence reaction rate?

prediction:

procedure:

Data: