Laksh Fine Chem Pvt Ltd.

(AN ISO??)

Standard operating procedure Sop no: CM 20 CALIBRATION PH METERS Issue number: 20

PROCEDURE Operate the instrument as per respective Standard Operating Procedure. Use Certified Standard pH buffer to set the instrument at pH 7.00 (with 7.00 pH buffer) and pH 4.00 (with 4.00 pH buffer) at 25o C. Take 100 ml buffer pH 4.0 & pH 7.0 from the Certified buffer bottle in a glass bottle. Discard the Standard pH buffer after one month. Prepare different pH solutions of 0.05 M Potassium biphthalate, 0.05 M equimolol phosphate, 0.01 M sodium tetraborate and Calcium Hydroxide (saturated solution at 25oC) Solution A : Preparation of 0.05 M Potassium Biphthalate (pH 4.01) Dissolve 10.12 gm. of KHC8H4O4 ( previously dried at 110 oC for 1 hour) in water to make 1000 ml. Solution B : Preparation of 0.05 M equimolol phosphate buffer( pH 6.86) Dissolve 3.53 gm. of Na2HPO4 and 3.39 gm. of KH2PO4 (each previously dried at 120oC for 2 hour) in water to make 1000 ml. Solution C : Preparation of 0.01 M Sodium tetraboarte buffer( pH 9.18) Dissolve 3.80 gm.of Na2B4O7.10 H2O in water to make 1000 ml. Protect the solution from absorption of carbon dioxide. Solution D : Preparation of Saturated solution of Calcium hydroxide at 25oC (pH 12.45) Shake an excess of calcium hydroxide with water and decant at 25oC before use. Protect the solution from absorption of carbon dioxide. (Prepare fresh solution when required) Check the pH of above buffer solutions. It should be within + 0.07. Record the value in the prescribed Annexure-II Check the pH of Certified buffer pH 4.0 & pH 7.0. It should be within +0.02 pH limit of labelled value. Record the value as per Annexure-II

Discard buffer solution A, B and C after three month. Frequency of calibration :- 15 days. Daily calibration Repeat the same procedure for daily calibration of pH meter using solution A, B and solution C and record value as per annexure-I If it is out of specified limit, follow the S.O.P. ABBREVIATIONS oC = Degree centigrade Na2B4O7.10H2O = Sodium Tetraborate Decahydrate Na2HPO4 = Disodium hydrogen orthophosphate KH2PO4 = Potassium dihydrogen orthophosphate KHC8H4O4 = Potassium Biphthalate ANNEXURE-I Q. C. DEPARTMENT DAILY CALIBRATION RECORD S.O.P.NO._________ NAME OF THE INSTRUMENT : pH METER MAKE : MODEL NO : IDENTIFICATION NO. : ______________________________________________________________ ________________ Primary standard : Certified buffer (pH 4.00 &pH 7.00) Date 0.05 M Potassium Biphthalate ( 4.01+ 0.07) 0.05 M equimolol phosphate ( 6.86+ 0.07) 0.01 M sodium Tetraborat e ( 9.18+ 0.07) Check point ( if applicable) Calibrated by Checked by

OBSERVATION CHART Q. C. DEPARTMENT CALIBRATION RECORD OF pH METER S.O.P.NO._________ NAME OF THE INSTRUMENT : pH METER MAKE : MODEL NO : IDENTIFICATION NO. : ______________________________________________________________ _________________ Primary standard : Certified buffer Primary standard : Certified buffer (pH 4.00 & pH 7.00) Batch No:___________ (pH 7.00) Batch No.:__________ (pH 4.00) OBSERVATION : Buffer Theoretical pH Observed Tolerance Remarks Value(At 25oC) value 0.05 M Potassium 4.01 + 0.07 Biphthalate 0.05 M equimolol 6.86 + 0.07 phosphate 0.01 M sodium 9.18 + 0.07 Tetraboarte Calcium hydroxide 12.45 + 0.07 (saturated) Standard Certified 6.98 + 0.02 buffer Standard Certified buffer 4.01 + 0.02

Managing director KantiBhai Madhani

Production Head