RESEARCH-SPECIFIC STANDARD OPERATING PROCEDURE (SOP)

PROCEDURE FOR WORK WITH ISOFLURANE GAS ( CAS # 26675467A) Using inhalation anesthesia system HAZARD CLASS: HALOGENATED ANESTHETIC GASES INHALATION EXPOSURE RISK INVESTIGATORS NAME: PHONE NUMBER: EMERGENCY PHONE #: 726-7273 OR 911 WASTE DISPOSAL #: 726-6764 1. Describe experiment/Research protocol: Be dressed with proper personal protective equipment: gloves, lab coat, eye protection Prior to using the anesthesia apparatus, tubing attachments and box integrity must be checked starting from the air flow source to the scavenging hood. If isoflurane reservoir is low or empty, position scavenging exhaust above anesthesia unit and carefully pour isoflurane from the stock bottle into the reservoir. Tighten stock bottle and reservoir lids. Return stock bottle to the chemical cabinet. Open tank valves for compressed air and oxygen tanks to provide carrier gas flow for anesthetic. Place animal, mouse or rat, into clear lucite flow through box. Introduce isofluane gas by opening flow to setting number 3. When animal is unconscious and toe pinch negative turn isoflurane flow off, move tubing from flow through box to nose cone, and transfer animal to procedure table with nose in nose cone. Turn isoflurane flow back on to setting number 2. Throughout procedure, monitor animal for response to toe pinch and eye touch, skin tone, respirations, and motion. Adjust isoflurane slightlly up/down as needed to maintain appropriate level of anesthesia. At end of procedure (animal has been euthanized or is ready to regain consciousness depending on IACUC protocol) turn isoflurane flow off, shut valves on compressed air and oxygen. 2. List substances to be used, including hazard class: Isoflurane CAS # 26675467A SOP APPROVAL DATE: EFFECTIVE DATE: IACUC PROTOCOL # IBC PROTOCOL #

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RESEARCH-SPECIFIC STANDARD OPERATING PROCEDURE (SOP)

Isoflurane is a halogenated anesthetic which is a clear, colorless, and highly volatile liquid at ordinary temperature and pressureThe National Institute for Occupational Safety and Health (NIOSH) has recommended that the average concentration of halogenated agents should not exceed 2ppm (15mg/m3) during any 1 hour period. A permissible exposure limit has not been established for anesthetic gases. This SOP addresses laboratory use and proper handling of isoflurane under the assumption that all laboratory staff have had the required safety training, and are in compliance with minimum laboratory safety requirements, as detailed in the latest version of the UMD Medical School-Specific Laboratory Safety Plan.

3. Describe potential hazards for each step, including physical and health hazards: The potential or exposure to isoflurane may occur if vapors escape into the work environment during the anesthetic administration process. Isoflurane stock bottles and anesthesia apparatus must be used only with a scavanging exhaust and only as described in the above protocol. Signs and symptoms of exposure: Acute exposure: nausea, vomiting, skin irritation, nose/throat/respiratory irritation, headache, dizziness, drowsiness, unconsciousness. Chronic exposure: hypotension, tachycardia, respiratory depressin, and elevated blood glucose level. 4. Identify and list personal protective equipment required and hygiene practices needed to prevent exposure and to conduct the experiment safely: Gloves, lab coats and eye protection such as chemical goggles or a face shield must be worn when handling stock bottles of isoflurane. When working with the isoflurane anesthesia for an animal protocol an N95 rated partical mask should be worn. 5. Describe engineering controls that will be used to prevent or reduce employee exposure to hazardous chemicals. This includes ventilation devices such as fume hoods: Isoflurane must be used with a waste anesthetic gas scavenging system. Upon request DEHS will evaluate scavenging systems used to control exposures to anesthetic gases and will evaluate personal exposure monitoring for laboratory workers. 6. Describe any special handling and storage requirements: Isoflurane must be stored in a cool well-ventilated location away from direct sunlight. A ventilated chemical storage cabinet is preferred. Laboratory door must be locked when authorized personnel are not present lab.

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RESEARCH-SPECIFIC STANDARD OPERATING PROCEDURE (SOP)

Isoflurane should be dated at receipt, entered into the laboratory chemical inventory, and should not be used beyond the expiration date on the label. Isoflurane stock bottle should be opened only in fume hood or under scavenging exhaust using PPE described in this SOP. 7. Describe how spills or accidental releases will be handled and by whom. List the location of appropriate emergency equipment (spill kit, eye washes, showers, and fire equipment). Any special requirements for personnel exposure should be identified here such as respiratory protection:
Personnel are advised of proper procedures for handling spills found at: http://www.d.umn.edu/ehso/emergencies, including contacting the lab PI or their designate to report spills. Locations of the eye wash and shower spill kits are described in the initial and annual lab refresher training. Spilled material will be collected and disposed of properly according to the Chemical Spill Clean up Guide: http://www.d.umn.edu/ehso/Spill_Cleanup_Guide.doc

DO NOT attempt to clean up large spills of isofluane. In the event of a spill, immediately evacuate the spill area and close all doors. From a safe place dial 9-1-1 to report the spill. Call the UMD Environmental Health and Safety Office (7273), describe the incident and request assistance. Alert all personnel in the immediate area to evacuate. If personal Injury occurs, call 911 immediately and request immediate assistance. 8. Specify decontamination procedures to be used: Anesthesia/animal procedure workplace in the lab should remain uncluttered. Before removal of PPE workplace should be wiped clean with mild soap and water. Wash hands thoroughly after glove removal. 9. Waste disposal Procedures. Explain how waste will be disposed and type of waste including contaminated lab-ware: Any unused stock isoflurane which reaches the expiration date will be labeled with the hazardous waste label, boxed, and disposed of through the UMD chemical waste program. 10. Additional Information: None

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RESEARCH-SPECIFIC STANDARD OPERATING PROCEDURE (SOP)

11. EMPLOYEE SIGNATURES This is to certify the employees named below have been trained on this SOP, have read and know the location of the department Laboratory Safety Plan and this SOP and understand the hazards and safe work practices as detailed in this SOP: Prior to starting work with isoflurane, the PI or his/her associate must provide training to his/her laboratory personnel specific to the hazards associated with working with isoflurane. The Principal Investigator or his/her associate must provide his/her laboratory personnel a copy of this SOP as well as a copy of the MSDS for isoflurane. The Principal Investigator or his/her associate must ensure that his/her laboratory personnel have completed initial laboratory safety training or refresher safety training within the last year. Note: The UMD Environmental Health and Safety Office staff is available to assist in the education and/or training of personnel concerning safe work practices. Also training may be taken online at: http://www.d.umn.edu/ehso/safety/lsptrain.html The Principal Investigator or his/her associate must ensure that his/her laboratory personnel have training in proper anesthesia system setup, operation, and maintenance

Name

Employee Id #

Initials

Principal Investigator Name: (Print): __ Principal Investigator (Signature):________________________________________ Date: _______________________________________________________________

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