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Our Innovation, Your Research — Shaping the Future of Life Science

Prestige Antibodies are the Most Highly ®
Human Protein Resource (HPR) Expression Profiles
Characterized Antibodies in the Industry Human Protein Atlas Image Gallery Location

Go to the Sigma product detail page, and click the Image Gallery to view the remarkable collection of protein expression images.

Validated by the Human Protein Resource (HPR) program for immunohistochemical

staining on 48 normal human tissues, 20 human cancer types, 59 cell lines and cells

■ Covering 3,600 protein targets
■ Validated by the Human Proteome
Resource (HPR)
■ Standardized in universal protocols
■ Over 700 IHC, IF and WB images
per antibody
■ All data conveniently searchable online
Prestige Antibodies 48 Normal Tissues, Immunohistochemistry 20 Cancer Tissues, Immunohistochemistry 59 Cell Lines, Immunocytochemistry
The Human Protein Atlas (HPA, proteinatlas.org) is a database that provides protein Protein expression profiles for each antibody are viewed in triplicate samples Protein expression profiles for each antibody are viewed in duplicated samples The HPA displays images of a selection of widely used and well characterized
expression profiles for human proteins, presented as immunohistochemical (IHC) images in normal tissues. Normal tissues are sampled from 144 (48 x 3) different in cancer tissues. Cancer tissues are derived from 216 (216 x 2) unique human cell lines as well as cell samples from normal individuals and
from most human tissues. The database not only delivers visible proof of antibody individuals annotated and curated by certified pathologist or by specially tumors individuals annotated and curated by certified pathologist or by leukemia/lymphoma patients. Duplicates from 47 cell lines and 12 samples of
performance, but also provides a knowledge base with regard to the structural and educated personnel. specially educated personnel. primary blood cells render a total of 118 images per antibody.
temporal expression proteins in various cells and tissues.
Prostate tissue Malignant glioma tissue Area based view representing
stained with Prestige stained with Prestige immunostained areas (%)
A systematic exploration of the human proteome is done by antibody-based proteomics,
Antibody anti-HSPA9 Antibody anti-MTHFD1 within the cells. Color
combining high-throughput generation of mono-specific antibodies with protein profiling (Cat. No. HPA000898) (Cat. No. HPA000704) code represents a range of
in a standard set of tissue microarrays (TMA) and cell microarrays (CMA). All tissue and cell immunoreactivity, yellow
samples are formalin-fixed and paraffin-embedded. (weak/moderate), green
(moderate/strong), and
Each antibody is used to explore the protein profile by IHC in 48 normal human tissues in red (strong).
triplicates, 20 cancer types from 4-12 individuals in duplicates, 47 cell lines and 12 primary
cell types. In total, more than 700 IHC images are generated for each antibody.

Placenta tissue Renal cancer tissue Object based view

To find out how you can search for Prestige Antibodies and view data on the HPA website, stained with Prestige stained with Prestige representing fraction
visit sigma.com/prestigedemo Antibody anti-HSPA9 Antibody anti-MTHFD1 (%) of immunostained
(Cat. No. HPA000898) (Cat. No. HPA000704) cells. Color code for
each represents range of
immunoreactivity, blue
Protein Class Coverage by Prestige Antibodies
(negative/very weak), yellow
(weak/moderate), green
(moderate/strong) and red
Kinases 34% (strong), Negative/very weak
areas are transparent.
Blood group
antigen proteins 31%
Colon tissue stained with Prestige Antibody anti-HSPA9 Breast Cancer tissue stained with Prestige Antibody Peripheral Blood Mononuclear Cells (PBMC)
SH2-domain
31% (Cat. No. HPA000898) anti-MTHFD1 (Cat. No. HPA000704) stained cells with Prestige Antibody anti-PDIA3
containing proteins (Cat. No. HPA003230)
CD markers 30%

SH3-domain
28% Immunofluorescent Analysis A B Western Blot
Protein Class

containing proteins

Enzymes 26% A large number of the Prestige Antibodies have been successfully used in Western Blot analysis of total protein extracts are presented for a large number
Immunofluorescence applications. Confocal microscopy images from three of the Prestige Antibodies. Western Blots are performed using a standard
Peptidase 24%
human cell lines, A-431, U-2 OS and U-251MG, are available on the Human sample setup composed of five different human protein lysates; cell-line RT-4,
GPCRs excl
22%
Protein Atlas. cell line A-431, plasma, liver and tonsil.
olfactory receptors

Transporters 21% Staining of the human cell line U-2 OS using the nucleoli specific Prestige Antibody anti-HMGB2
Image A. Vasodilator-stimulated phosphoprotein, (Cat. No. HPA005724)
(Cat. No. HPA003506). The four-color image is acquired in four separate channels that can be
Transcription factors 18% viewed separately or in different combinations. Image B. Four and a half LIM domains protein 2, (Cat. No. HPA006028)
Image A. Staining of nucleoli in green. Image C. Flotillin-1, (Cat. No. HPA001393)
Ribosomal proteins 16%
Image B. Staining of nuclei (blue) and the anti-HMBG2 Prestige Antibody (green). C D Image D. Cleavage Stimulation Factor 64 kDa Subunit, (Cat. No. HPA000427)
0% 5% 10% 15% 20% 25% 30% 35% 40%
Image C. A third channel is turned on for a three-color overlay view of cytoskeleton (red),
Percent Coverage nuclei (blue) and the anti-HMBG2 Prestige Antibody (green).
Image D. The fourth channel is turned on for a four-color overlay view of endoplasmic reticulum
(yellow), cytoskeleton (red), nuclei (blue) and the anti-HMBG2 Prestige Antibody (green).

Our Innovation, Your Research — Shaping the Future of Life Science 7

sigma.com/lifescience ORDER: 800-325-3010 TECHNICAL SERVICE: 800-325-5832 Our Innovation, Your Research — Shaping the Future of Life Science