DMLT 13 Batch

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Basic Laboratory Technology

LABORATORY CALCULATIONS Tutorial

1. At 15C the density of HCl is 1.1889g/mL. Determine its specific gravity at that temperature. 2. At 19C a urine specimen has a specific gravity of 1.028.This measurement was made with a hydrometer that had been calibrated at 25C. What is the corrected specific gravity of the urine? 3. How many grams of compound are required for the specified solutions? a. CaCl2 for 500mL of 0.05mol/L solution b. HNO3 for 5 litres of 2.0mol/L solution 4. What is the molarity of a solution that contains 17.7g of KCl in 500mL? 5. How many grams of Na2SO4 are required to prepare 0.4L of a 2.0mol/L solution? 6. How many grams of Na2SO4 are required to prepare 0.4L of a 2.0mmol/L solution? 7. How many grams of NaOH are required to prepare 50mL of a 0.05mol/L solution? 8. How many grams of MgCl2.6H2) would you use to prepare 1.0decilitre of a 50mmol/L? 9. How many mL of conc HNO3 (SG 1.42: purity 70%) must be diluted to give 250mL of a 1.25mol/L solution? 10. A laboratory procedure calls foe 250mL of a 2% suspension of red cells. A 50% suspension is available. How much of the 50% suspension is needed, and how much of diluent is required? 11. 1% solution of HCl is required for a procedure. A 5% solution is available. How much of the 5% solution is needed to make up 500mL of a 1% solution? 12. A buffer is prepared by adding 2 parts of a reagent A to 7 parts of reagent B. A total of 45mLa of buffer are required. How much of reagent A and B are needed? 13. A 1L of 70% alcohol is required. How much of 95% alcohol is required to make the 70% solution? 14. 200mL of 2% acetic acid are required. How many mLs of 5% acetic acid are required to make the 2% solution? 15. How would 100mL of a 10% solution of NaCl be prepared? 16. How would a 300mL solution of 5% acetic acid be prepared from conc(glacial) acetic acid? Glacial acetic acid is 99.7% pure. 17. How many grams of NaOH are required to prepare 2.00L of a 0.500N solution? 18. Given a 4.0 normal NaOH solution, what is the molarity of the solution? 19. Determine the molarity of the following solution. a. 2.50moles NaCl in 1.00L of a solution.

b. 8.40g of NaHCO3 in 250mL of solution. 20. How to prepare 5ml of solution

Department of Biomedical Sciences/FHS/MNU

DMLT 13 Batch

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How much aliquot is required to prepare 8ml of 1:20 dilution? How much diluent is required to prepare 8ml of 1 in 20 dilution A series of dilutions that were performed as follows: Tube1: 1/2 Tube 2: 1/4 Tube 3: 1/8 Tube 4: 1/16 a. Calculate the following: b. What is the final dilution? c. What is the final dilution factor? d. What is the dilution factor of tube 3?

24. If there is a 6.00M NaOH solution but a 5.00M solution is needed, how much water should be added to the 6.00M solution to dilute it to 5.00M? 25. How is 100mL a 20.0% w/v solution of MgSO4 prepared? 26. How is a 10.% v/v solution of glacial acetic acid prepared using de ionized water as the solvent?

Department of Biomedical Sciences/FHS/MNU