6 Photosynthesis

BIOSCI.101
This course has six labs: 1. Enzymology 2. Gene Expression 3. Genetics

- LAB

4. Evolution
5. Blood Glucose

6. Photosynthesis

Lab 6

Ph Photosynthesis h i

Plants are important!!!

Nearly everything on the planet depends on energy produced by photosynthesis

Produces the O2 in the atmosphere

Photoautotrophs
Produce energy through photosynthesis (plants, algae, bacteria)

Why leaves are green.

.is due to the interaction of light with the chloroplasts

The electromagnetic spectrum

Photosynthesis equation
Reactants Products
light

6CO2 + 6H2O

C6H12O6 + 6O2

Photosynthesis is split into two distinct reactions

Light reactions photo Dark reactions synthesis Calvin cycle

Tracking atoms through photosynthesis

The location of photosynthesis

Chloroplasts

Leaf cross section

Overview of photosynthesis (1)

H2O is oxidised

Overview of photosynthesis (2)

Overview of photosynthesis (3)

What molecule absorbs light?

Chlorophyll

Chlorophyll is located in the thylakoid membrane

Chlorophyll absorption spectrum

Evidence that chloroplast pigments are involved in photosynthesis h t th i

How a photosystem harvests light

Primary electron acceptor Electron transfer Reaction center chlorophyll Reaction Center

Pigment molecules

Photosystem

Photosystems and electrons

There are two photosystems PSI and PSII There are two types of electron flow acyclic and cyclic Acyclic involves PSII and PSI Cyclic involves PSI only

ePSII

PSI PSII

PSI

e-

How non-cyclic electron flow generates ATP and NADPH

Primary e- acceptor P im Primary e- acceptor t 1 H2O 2 O 2 2eLight Photosystem II
P680

3 Pq

Fd

2e-

NADP+ reductase
NADP+ NADPH

2e-

Cytochrome complex 4
e- flow provides energy for f synthesis of

Pc

P700

ATP

Photosystem I

Light

Mechanical analogy..

Cyclic electron flow just generates ATP

Primary e- acceptor P im Primary e- acceptor t Pq H2O O2 2e2eP680

Fd Fd 2eNADP+ reductase
NADP+ NADPH

Cytochrome complex
e- flow provides energy for f synthesis of

Pc

P700

Light Photosystem II

ATP

Photosystem I

Light

Todays lab.
Measuring the initial velocity of acyclic electron flow INSTEAD of NADP+ as an electron acceptor we are using the dye DCPIP (pg 6.1 & pg 6.2) Use class results to determine the relationship between electron flow and light intensity Table on pg 6.3 unknown vol = 120ul Sharing specs again READ INSTRUCTIONS

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Graphs
1. Abs Draw a line through steepest p part p Light exposure (mins) 2. From class averages Initial I iti l velocity of e- flow dec lamp dist REMEMBER: Us pencil Use il Think about your units

Absorbance
In spectroscopy the absorbance A is defined as: A= -log log (I/I0) I = transmitted light intensity intensity of light at a specified wavelength () that has passed through a sample I0 = incident i id t li light ht i intensity t it intensity of the light before it enters the sample EXPLAINED by the BEER-LAMBERT LAW

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Determining an absorption spectrum

Alternative learning style

youtube video video... http://nz.youtube.com/watch?v=_IVE68rh18...

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MASTER SLIDE
Copy and use to make your own slides

Assignment - name; stream number;

bench (#)

FINAL CHECKLIST:

Clean Up instructions on
whiteboard. XXX xxxxxxxxxxxxxxxxx Wash hands before leaving

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