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Approvals
Many of the commercial antibiotic residue screening test kits have been formally approved or certified by regulatory bodies or internationally recognised organisations that undertake evaluations, validations and/or performance testing of test kits. Note that DFSV does not perform approvals or certifications of test kits. When choosing a commercial antibiotic residue screening test kit, it is important to check that it has appropriate approval or certification. For each of the antibiotic residue screening test kits shown in Table 1, their relevant approval or certification is listed.
Sensitivities
Sensitivities (also referred to as detection limits) vary between each of the commercial screening test kits for the different antibiotic residues. These different sensitivities should be considered carefully when deciding which test kit to use. For further details on the sensitivities of each of the kits listed in the table, refer to the technical information note Detection limits of antibiotic residue screening test kits.
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Other factors
Other important factors to consider when choosing which commercial test kit to use are: The time taken to yield a result ease of use the cost of set-up equipment and consumables. It is suggested that the Australian test kit distributor be consulted to provide this information.
Further information
Other technical information notes on antibiotics and dairy food safety topics are available at www.dairysafe.vic.gov.au Or contact Dairy Food Safety Victoria on (03) 9810 5900 or info@dairysafe.vic.gov.au
Copyright Dairy Food Safety Victoria 2013
This document is intended to be used as a general guide only and is not a comprehensive statement of all the relevant considerations with respect to your particular circumstances, nor does it comprise, or substitute for, legal or professional advice. DFSV does not guarantee the accuracy, reliability, currency or completeness of the information. DFSV accepts no legal liability arising from, or connected to, any reliance on this document.
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Table 1: Antibiotic residue screening test methods available for use in Australia
Test method BetaStar Charm II 1 Australian distributor CHR Hansen Pty Ltd Merck Pty Ltd Antibiotic classes detected Beta-lactams and cephalosporins Beta-lactams, cephalosporins, sulfa drugs, tetracyclines, macrolides, Aminoglycosides, others (novobiocin, chloramphenicol, thiamphenicol and dapsone) Sulfonamide, Sulfadimethoxine, Sulfamethazine Current certifications/approvals AFNOR, USFDA and AOAC Argentina, AOAC, NSW Food Authority and USFDA4
Suitable matrices* Raw cow/sheep/goat milk Raw cows milk and liquid milk. Goat/ sheep milk, cream, condensed milk, whey and dairy powders Raw cows milk, liquid milk and permeate. Goat/sheep milk, colostrum, cream and condensed milk
Merck Pty Ltd Charm ROSA MRL Sulfonamide, Sulfamethoxine (SDSM), Sulfamethazine (SMZ)2 Cowside II Merck Pty Ltd
AOAC, USFDA
Beta-lactams, cephalosporins, sulfa drugs, tetracyclines, macrolides, aminoglycosides, others (chloramphenicol, thiampheniicol, trimethoprim and dapsone) Beta-lactams, Cephalosporins, sulfa drugs, tetracyclines, macrolides, Aminoglycosides, others (chloramphenicol, thiamphenicol, trimethoprim and dapsone) Beta-lactams, cephalosporins, sulfa drugs, tetracyclines, macrolides, Aminoglycosides, others (chloramphenicol, trimethoprim and dapsone) Beta-lactams and cephalosporins Beta-lactams and cephalosporins
AOAC, USFDA
Raw cows milk, liquid milk and permeate. Goat/sheep milk, colostrum, cream and condensed milk Raw cows milk and liquid milk. Goat/ sheep milk and milk powders
Copan
Delvotest SPNT
AOAC, MPI NZ, NSW Food Authority, Germany, UK, Argentina, Denmark and Switzerland
Raw cows milk Raw cows, sheep and goat milk Raw cows, sheep and goat milk Raw cows milk. Goat/sheep milk and other dairy products
Ballya SensorBT
None
Disc Assay3
Not applicable Penicillin and non-specific for other beta-lactams and non-beta-lactams
Not applicable
All sensitivities are based on those declared by kit manufacturers/distributors Australian information brochures and are current as of the date of this publication. These sensitivities correspond to when the kits are used according to the kit manufacturer/distributor instructions to give maximum sensitivities (e.g. for Delvotest SP-NT- running the kit at control time). KEY * Suitable matrices listed are obtained from manufacturers written materials only. Manufacturers are advised to contact distributors for suitability of other matrices of interest that are not listed. Matrices other than raw cows milk and liquid milks generally require pre-treatment of samples prior to testing. Refer to the test kit distributor for further details. Charm II systems also allow for semi-quantitative analysis. The SMZ test detects sulfamethazine only. Can be used as qualitative and quantitative methods for penicillin G. Approval applicable for particular Charm II test kits only.
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Abbreviations: AOAC AOAC International AFNOR Association Francaise de Normalisation, MPI NZ Ministry for Primary Industries New Zealand, NSW New South Wales, USFDA The United States Food & Drug Administration. Note: All of the information provided in this table for antibiotic classes detected, current certifications/approvals and suitable matrices is based on the information provided by kit manufacturer/distributors Australian information brochures.