The effect of pH on enzymatic activity:

Table: Optical density over time for pH 3, 5, and 7.

Time pH
3 5 7
0 0.207 -0.100 0.056
15 0.226 -0.100 0.091
30 0.245 -0.890 0.135
45 0.263 -0.060 0.172
60 0.281 -0.035 0.202
75 0.298 -0.014 0.228
90 0.313 0.007 0.255
105 0.328 0.022 0.272
120 0.342 0.036 0.287
135 0.355 0.048 0.302
150 0.367 0.058 0.315
165 0.379 0.068 0.327
180 0.389 0.074 0.337
195 0.400 0.080 0.347
210 0.410 0.085 0.355
225 0.419 0.090 0.364
240 0.427 0.093 0.371
255 0.435 0.095 0.377
270 0.442 0.098 0.383
285 0.449 0.100 0.389
300 0.455 0.102 0.393
Observation: Color of mixture in cuvette after Spectrophotometric measurement
pH 3: caramel color
pH 5: light orange color
pH 7: light orange-brown