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Micropropagation of kiwifruit using non-axenic shoot tips - Springer

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Micropropagation of kiwifruit using non-axenic shoot tips

Paul L. Monette Look Inside Get Access

Abstract
Kiwifruit (Actinidia chinensis Planch.) shoot tips were subjected to a standard surface sterilization procedure
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Micropropagation of kiwifruit using non-axenic shoot tips - Springer

and cultured on a Murashige and Skoog basal medium in the presence of two surviving bacterial contaminants. The fresh weight increase of the cultures and the number of shoots produced were greater in liquid medium than in medium solidified with 0.4 or 0.8% agar. A greater number of shoots was obtained with 125 ml than with 50, 250, or 500 ml Erlenmeyer flasks. A concentration of 2 mgl-1 N6-benzylaminopurine (BAP) gave a greater increase in fresh weight than either 0 or 4 mgl-1. Shoots cut from proliferating cultures were dipped in 0.05% indolebutyric acid (IBA) and rooted directly in a peat: vermiculite: perlite mix. Over 93 % of 907 plantlets produced were successfully acclimatized. The productivity of the method was comparable to that reported for the axenic culture of meristems. The contaminants which survived the initial surface sterilization procedure thus presented no major obstacle to the in vitro propagation of kiwifruit. Page %P Close Plain text

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Micropropagation of kiwifruit using non-axenic shoot tips - Springer

References (23) About this Article

Title Micropropagation of kiwifruit using non-axenic shoot tips Journal Plant Cell, Tissue and Organ Culture Volume 6, Issue 1 , pp 73-82 Cover Date 1986-01-01 DOI 10.1007/BF00037760 Print ISSN 0167-6857 Online ISSN 1573-5044 Publisher Martinus Nijhoff, The Hague/Kluwer Academic Publishers Additional Links Register for Journal Updates Editorial Board About This Journal Manuscript Submission Topics Plant Sciences Plant Physiology Keywords Actinidia chinensis Aerococcus Bacillus fastidiosus liquid medium size of flask Industry Sectors Chemical Manufacturing Consumer Packaged Goods Energy, Utilities & Environment Authors
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Micropropagation of kiwifruit using non-axenic shoot tips - Springer

Paul L. Monette (1) Author Affiliations 1. Saanichton Research and Plant Quarantine Station, Agriculture Canada, 8801, East Saanich Road, V8L 1H3, Sidney, British Columbia, Canada

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