PRODUCT DATASHEET

Illumination Units
LEDs for Life
CoolLEDs pE-200 & pE-300 series LED illumination units are designed to be a direct replacement to the high pressure mercury lamp previously used on fluorescence microscopes. The pE-200 can be specified with two LED wavelength combinations. The pE-300 is available with three standard LED wavelengths. With a large range of microscope adaptors, the pE-200 & pE-300 can be fitted to most current and older microscopes. The result is a safe, convenient light source which should last for many years without any additional operating costs.

KEY FEATURES
Instant on/off Long Lifetime Controllable No replacement bulbs required No hazardous materials for disposal Reduced photobleaching No unwanted UV Better contrast sharper images See deeper into your cells Individual intensity control: 0-100% in 1% steps

LIFETIME GRAPH

LED / FLUOROPHORE SELECTION

365

470

530

Typical Fluorophores:

Typical Fluorophores:

Typical Fluorophores:

DAPI Calcofluor White Hoechst

and other like fluorophores

GFP FITC Auramine Alexa 488 Acridine

and other like fluorophores

TRITC Rhodamine Spectrum Green Cy3 RFP

and other like fluorophores

APPLICATIONS

OPERATION
Manual control from remote Control Pod Instant On/Off Independent 0-100% intensity control for each LED wavelength Single mode for one LED wavelength Group mode for multiple wavelengths

TECHNICAL DATA
Operating life: >10,000 hours On time Typical output powers: 365nm : 200 mW 470nm : 660 mW 530nm : 610 mW
*Performance values may increase in future. Check CoolLEDs website for latest data.

Routine Screening Tissue Culture Clinical Diagnostics Confocal

Input: 100-240V AC, 50/60 Hz, 2 Amps

FILTER RECOMMENDATIONS
In most cases your filter cubes will be suitable for LED illumination. CoolLED can assist in the selection of the optimal filters when requested. Existing Microscopes: To ensure the best possible match between LED illumination and the filter set in the microscope, CoolLED has identified some possible filter sets available from popular filter manufacturers. These are shown below. Wavelength Chroma Semrock Omega
365 470 530 11000v3 11001v2 11007v2 DAPI-5060C-000 FITC-LP01-Clinical-000 Cy3-4040C-000 XF02-2 XF100-2 XF101-2

New Microscopes: CoolLED can advise on selection of suitable new filter cubes provided by leading microscope manufacturers such as AccuScope, Leica, Leitz, Nikon, Olympus, Motic and Zeiss. Please visit our website for further details at: http://www.coolled.com/Life-Sciences-Analytical/Clinical-Products/Filter-Recommendations/

ORDER/OPTIONS
The pE-200 and pE-300 can be fitted directly to the existing epi-fluorescent port of a microscope. You can specify the wavelengths, adaptor and power cable using the codes below.
Fluorophore pE-200 DAPI/FITC FITC/TRITC DAPI/TRITC pE-300 DAPI/FITC/TRITC Wavelength 365/470 470/530 365/530 365/470/530 Product Code pE-200D-CON1-YYY-ZZ pE-200D-CON2-YYY-ZZ pE-200D-CON3-YYY-ZZ pE-300D-CON1-YYY-ZZ

DIMENSIONS

162mm 146mm 186mm POD

37mm

Direct-fit to microscope

68mm 77mm

Specify microscope adaptor YYY from CoolLEDs extensive list. See website: http://www.coolled.com/Life-Sciences-Analytical/Clinical/Microscope-Adaptors/ Specify local power cable ZZ. 10 Australia, 20 Europe, 30 UK, 40 USA

Indirect-fit to microscope
Options will be available in the future with light guides and fibers.
88 m m

Pod unit: Weight = 315g pE-300 unit: Weight = 1.40Kg

m 5m 12

CONTACT
Online: www.coolled.com Phone: +44 (0) 1264 323040 (Worldwide) 1 800 877 0128 (USA + Canada) Email: info@coolled.com Office: Westmarch Business Centre, River Way, Andover, SP10 1NS, UK

www.CoolLED.com
pE-200 & pE-300 Product Datasheet - September 2012

68mm