Name______________ Date______________ Period______________ DNA Extraction Lab Overview: In this lab, you will observe the hysical ro erties

o! DNA" #ou will brea$ o en strawberry cells, re are a !iltered extract containin% strawberry DNA, and se arate out molecules o! DNA in a test tube" &hat does DNA loo$ li$e in a test tube' &hat ro erties can be observed with the unaided eye' Introduction (trawberries are a %ood source o! DNA because they have ) co ies o! each ty e o! chromosome" #ou will brea$ o en the cells o! the strawberry, and then se arate the DNA !rom the remainin% cell arts" #ou will never be able to eat a strawberry a%ain without thin$in% about how much DNA is in it* Pre+,uestions: -" &hat are chromosomes made o!' ." &here are chromosomes !ound in a cell' /" 0ow many chromosomes do humans have' 1" &hy would a strawberry have DNA'

Observations &hat did the DNA loo$ li$e' Ex lain in detail" Is this what it loo$s li$e in boo$s' Draw a icture o! what the DNA loo$s li$e 2with the na$ed eye3 while still in the test tube" 2/ oints3

,uestions 2answer in 4O5PLE6E sentences3 &hy did you smash u the strawberry' &hat was ha

enin% to the cells' 2. oints3

DNA is soluble 2can dissolve3 in water, but not ethanol" &hy did you use ethanol' Ex lain what ha ened when the ethanol came in contact with the strawberry extract" 2/ oints3

A erson can not see a sin%le cotton thread -77 !eet away, but i! thousands o! threads are wound to%ether on a ro e, the ro e can be seen !ar away" 0ow is this statement an analo%y to the DNA extraction you did' 20int: 0ow could we see the DNA i! it is soooooooo small'3 2. oints3

In order to study human %enes, scientists must !irst extract human DNA" &ould you %et DNA !rom a human the same way you %ot DNA !rom a strawberry' &hy or why not' Ex lain 21 oints3

&hy mi%ht a scientist want to study strawberry DNA' List . ossible reasons" 2. oints3

Extra credit Draw what the DNA loo$s li$e under a microsco e slide" 8se color and a detailed drawin%"

6ry to extract DNA !rom another !ruit" Is the DNA the same' Is it di!!erent' Ex lain your answer" Draw what it loo$s li$e with the na$ed eye"

Strawberry DNA Extraction Lab Directions Materials: 9i loc :a% (trawberry -7 ml DNA extraction bu!!er 2soa y, salty, water3 6est tube Piece o! cheesecloth 6est tube rac$ 2- er table3 ;lass stirrin% rod (a!ety ;lasses Ethanol 2or iso ro yl alcohol3 ;loves ;raduated cylinder <unnel

Procedure -" Put on your %o%%les and %loves ." Place - strawberry in a 9i loc ba%" Press the air out o! the ba% and seal it" ;ently mash the ba%%ed strawberry with your hand !or at least . minutes until it is a ul " /" Add the -7 ml o! DNA extraction bu!!er to the ba% 2- i ette=.mL3" Press out all o! the air and seal the 9i loc" 4are!ully mash the ba% !or at least another minute 2>you do not want to ma$e a lot o! bubbles3" 1" Place your test tube in a test tube rac$" Place the !unnel inside the test tube" (tretch a iece o! cheesecloth on to o! the !unnel" ?" 4ut the bottom corner o!! your 9i loc ba%" 4are!ully s@ueeAe the strawberry ul on to o! the cheesecloth 2>you may need to have someone hold the cheesecloth in lace3" 6he strawberry Buice should drain into the test tube" &hen the test tube is about C !ull o! Buice, throw the ul and cheesecloth away" D" (LO&L# driAAle 4OLD ethanol 2or iso ro yl alcohol3 down the (IDE o! the test tube, until the test tube is about E !ull" F" Di the stirrin% rod into the test tube until it reaches the oint where the ethanol and strawberry Buice meet" Loo$ at the test tube at eye level" ;ently twirl the stirrin% rod and watch what ha ens" )" Loo$ at the DNA and observe the characteristics" #ou may scra e the DNA o!! and ut it onto a slide and loo$ at it under the microsco e"