40th COSPAR Scientic Assembly 2014

Life Sciences as Related to Space (F) Molecular, Cellular and Synthetic Biology Research in Space and Ground-based Analogues (F5.5)

ANTIRADIATION UV VACCINE: UV RADIATION, BIOLOGICAL EFFECTS, LESIONS AND MEDICAL MANAGEMENT - IMMUNE-THERAPY AND IMMUNEPROTECTION.

Dmitri Popov, dlpopov@rogers.com Advanced Medical Technologies and Systems, Richmond Hill, Canada Jerey Jones, jajones@bcm.edu Baylor College of Medicine, Houston, United States Slava Maliev, niobiot@mail.ru Russian Academy of Science, L4E 0R3, Canada

Keywords: Ultraviolet radiation,Standard Erythema Dose(SED), Minimal Erythema Dose(MED), Sun Burns, Solar Dermatitis, Sun Burned Disease, DNA Damage,Cell Damage, Antiradiation UV Vaccine, Immune-Prophylaxis of Sun Burned Diseases, Immune-Prophylaxis of Sun Burns, Immune-Therapy of Sun-Burned Disease and Sun Burns,Basal Cell Carcinoma (BCC), Squamous Cell Carcinoma (SCC), Toxic Epidermal Necrolysis(TEN). Introduction: Sun and high doses of UV generated by articial sources create an exposure of mammals and biological objects by ultraviolet(UV)radiation. UV radiation belongs to the non-ionizing part of the electromagnetic spectrum and ranges between 100 nm and 400 nm;100 nm has been chosen arbitrarily as the boundary between nonionizing and ionizing radiation. UV radiation is conventionally categorized into 3 areas: UVA (>315400 nm),UVB (>280315 nm)and UVC (>100280 nm)[IARC,Working Group Reports,2005] Stratospheric ozone levels dramatically lowering for past decades so current ultraviolet-B (UV-B) radiation levels are thought to be close to their maximum. An important consequence of stratospheric ozone depletion is the increased transmission of solar ultraviolet (UV)radiation to the Earths lower atmosphere and surface [S.Madronich et al.1998] Overexposure of ultraviolet radiation a major cause of skin cancer including basal cell carcinoma (BCC), squamous cell carcinoma (SCC) collectively referred to as non-melanoma skin cancer (NMSC) and melanoma is the most common cancer in North America. [Armstrong et al. 1993, Gallagher et al. 2005] Methods and Experimental Design: Our experiments and testing of UV Antiradiation Vaccine have employed a wide variety of laboratory animals which include : Chinchilla rabbits, 11-12 months old, live weight 3.5-3.7 (n=11), Balb mice, 2-3 months old, live weight 20-22 g (n=33), Wistar rats, 3-4 months old, live weight 180-220 g(n=33).Seven rabbits, ten mice,eleven Wistar rats were vaccinated with UV Antiradiation Vaccine.The second group of animals was used as biological control without UV Radiation and other, third group of animals was used as control without any interventions

before and after UV Radiation. Vaccination with UV Antiradiation Vaccine were provided in 17 days before UV irradiation.The animals were irradiated in DRT-1 UV generator lump. Dose of irradiation for laboratory, experimental animals was 10-12 * Standard Erythema Dose (SED) at L=283,7 . Laboratory animals were placed in to the box with ventilation. Results: Ultraviolet Irradiation of the skin was performed with high doses and causes an inammation or erythema in all experimental animals. However grade of skin damage and inammation was different between protected by vaccination and non-protected, non-vaccinated animals. Animals without UV Antiradiation Vaccine protection suered from extensive sunburns of second-third degree of sun burns. However, animals protected with UV Antiradiation Vaccine demonstrated much mild forms of cell injury - rst degree ans small putches with second degree of skin injury. Discussion: The severity of skin damage depended on area of exposed skin, time of UV irradiation and doses of UV irradiation. Skin damage could be divided for 4 major grades: 1. Faint erythema with dry desquamation. 2.Moderate to severe erythema. 3. Severe erythema with blistering, moist desquamation. 4. Toxic epidermal necrolysis. Mild doses of UV radiation induce cell death by apoptosis and moderate and high doses of UV radiation induce cell death by necrosis and generate systemic inammatory response syndrome (SIRS), toxic multiple organ injury (TMOI), toxic multiple organ dysfunction syndromes (TMODS),and nally, toxic multiple organ failure (TMOF).[D.Popov et al.2012,Fliedner T.et al. 2005,T. Azizova et al. 2004] UVB is a complete carcinogen that is absorbed by DNA and directly damage DNA. DNA damage induced by UVB irradiation typically includes the formation of cyclobutane pyrimidine dimers(CPD) and 6-4 photoproducts (6-4P)[IARC, Working Group Reports, M.Saraiya et al. 2004]. Conclusion: Antiradiation UV Vaccine most eective agent for prophylaxis and treatment of acute sun burns and sun burn toxicity. Antiradiation UV Vaccine could be used in cooperation with antioxidants and prevent all forms of UV radiation toxicity. Authors of this experiments assume that Antiradiation UV Vaccine could be eective technology for prophylaxis and prevention of dierent forms skin cancer.