Amplification Consumble

Amplication Reagents and Plastics
Amplication: Consumables
Factors Impacting Gene Expression Analysis
RNA Isolation

RNA integrity, purity, and yield

Genomic DNA contamination

Inhibitors of cDNA synthesis and qPCR

RNase and DNase contamination
Reagents Reverse Transcription

cDNA synthesis efciency

RNA protection

Input RNA capacity

Accurate representation of mRNA
Reagents Real-Time qPCR

Detection sensitivity

Assay specicity

Inhibitors in sample

Reproducibility of thermal cycling conditions and instrument compatibility
PCR Plastic Consumables

Instrument compatibility

Optimum performance

Automation friendly

Potential source of contamination and inhibition
RNA Isolation

Kits are designed and formulated to assist
in the isolation of highly pure and intact RNA
from different starting materials

RNA is compatible with a variety of
downstream applications
Real-time qPCR
Northern blotting
Microarray analysis
cDNA library construction

DNase treatment ensures genomic DNA removal
2012 Bio-Rad Laboratories, Inc. Amplification Reagents and Plastics 5
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732-6800, 2 x 96-well preps
Aurum Total RNA 96 Kit
Aurum
Total RNA Fatty and Fibrous Tissue Kit

PCR-ready RNA in less than 60 min

PureZOL
efciently lyses cells and tissues, deproteinates RNA, and

inactivates endogenous nucleases in a single step

High yield of intact total RNA from difcult-to-disrupt samples, including plant
and animal tissues

Well suited for fungal samples that are rich in RNases

RNase-free reagents and plastic consumables ensure the integrity of
isolated RNA

Kit includes DNase I for removal of genomic DNA contamination

Easy-to-use spin or vacuum protocol
For more information, request bulletin 5282.
Aurum Total RNA Mini Kit

PCR-ready RNA in less than 60 min

Guanidine isothiocyanate and -mercaptoethanol efciently lyse samples
and quickly inactivate RNases

High yield of intact total RNA from a wide range of starting materials, including
cultured cells, bacteria, and yeast, as well as plant and animal tissues

isolated RNA


Easy-to-use spin or vacuum protocol
Aurum Total RNA 96 Kit

High-throughput total RNA isolation in less than 60 min

High yield of intact total RNA from a wide range of starting materials,
including cultured cells, bacteria, and yeast, as well as plant and animal tissues

Guanidine isothiocyanate and -mercaptoethanol efciently lyse samples
and quickly inactivate RNases

isolated RNA


Compatible with Aurum vacuum manifold
RNA Isolation
732-6820, 50 preps
Aurum Total RNA Mini Kit
732-6830, 50 preps
Aurum Total RNA Fatty and Fibrous Tissue Kit
www.bio-rad.com/
rna-isolation
Amplification Reagents and Plastics Visit us on the Web at www.bio-rad.com. 6
iScript RT-qPCR sample preparation reagent generates linear
results over varying input cell amounts. HeLa cells (125, 25, 5, and
1 cells/l) were treated and analyzed for GAPDH expression levels
using iScript cDNA synthesis kit and iQ
SYBR
Green supermix
on the CFX96
real-time PCR detection system. RFU, relative

uorescence units.
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iScript
RT-qPCR Sample Preparation Reagent

Reagent stabilizes RNA and removes genomic DNA in less than 10 min

Suitable for adherent or suspension animal cells

RT-qPCR is directly enabled from cells without RNA purication when
combined with an iScript reverse transcription kit and real-time supermix

Reagent allows multiplex real-time detection of up to 4 targets from as few
as 10 cells

Ideal for rapid, high-throughput gene expression analysis
PureZOL
RNA Isolation Reagent

Single-solution format permits recovery of RNA from small quantities of
tissues or cells, making it ideally suited for gene expression studies

Efcient RNA purication from cultured cells, yeast, viruses, and bacteria,
as well as plant and animal tissues

PureZOL efciently lyses cells and tissues, deproteinates RNA, and
inactivates endogenous nucleases in a single step

Scalable starting sample amount

Convenient isolation of RNA, DNA, and protein from the same sample
Aurum
Vacuum Manifold

Vacuum-mediated nucleic acid purication platform

Versatile manifold format adaptable for 96-well plate or up to
18 spin columns

Manifold ensures fast, high-quality sample preparation while
maintaining the simplicity of vacuum processing

Unique vacuum regulator design allows for complete control of
negative pressure
170-8899, 5 x 10 ml
iScript RT-qPCR Sample Preparation Reagent
732-6890, 100 ml
PureZOL RNA Isolation Reagent
RNA Isolation
732-6470, 1 unit
Aurum Vacuum Manifold
www.bio-rad.com/
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Aurum
Total RNA Kits PureZOL
RNA
Mini Fatty and Fibrous Tissue 96 Isolation Reagent
Format Mini column Mini column 96-well plate Single solution
Filtration (vacuum or spin) Filtration (vacuum or spin) Filtration (vacuum or spin) organic extraction
Maximum starting
material amounts
Cultured cells 2 x 10
6
1 x 10
7
1 x 10
6
1 x 10
7

Bacterial cells 2.4 x 10
9
2.4 x 10
9
8 x 10
8
2.4 x 10
9

Yeast cells 3 x 10
7
3 x 10
7
2 x 10
7
3 x 10
7

Hard animal tissue 20 mg 100 mg 100 mg
Soft to moderately 40 mg 100 mg 100 mg
hard animal tissue
Plant tissue 40 mg 100 mg 100 mg
Isolation method Silica membrane Lysis with PureZOL reagent, Silica membrane Organic extraction
purication on silica membrane
Number of preps 50 mini preps 50 mini preps 2 x 96-well plate 50 or 100 (1 ml/prep)
Number of washes 3 3 3
DNase I included* Yes Yes Yes No
DNase I digest time 15 min (animal tissue, 25 min) 15 min 10 min
Total preparation time** <5080 min (with DNase I digest) <5080 min (with DNase I digest) <60 min (with DNase I digest) <60 min
Binding capacity >100 g >100 g >40 g
Elution volume 2 x 40 l 2 x 40 l 80 l 30100 l
* Removal not required.
** Total preparation time will vary depending on the tissue or cell type and on which format is used (vacuum or spin).
For sample-specic yield information, please visit www.bio-rad.com/rna-isolation and click the RNA Isolation Selection Guide.
RNA Isolation
Selection Guide
Ordering Information
Catalog # Description Catalog # Description
732-6830 Aurum Total RNA Fatty and Fibrous Tissue Kit 170-8899 iScript RT-qPCR Sample Preparation Reagent, 500 reactions
732-6870* Aurum Total RNA Fatty and Fibrous Tissue Module 732-6880 PureZOL RNA Isolation Reagent, 50 ml
732-6820 Aurum Total RNA Mini Kit 732-6890 PureZOL RNA Isolation Reagent, 100 ml
732-6800 Aurum Total RNA 96 Kit 732-6470 Aurum Vacuum Manifold
170-8898 iScript RT-qPCR Sample Preparation Reagent, 100 reactions

* Not provided with PureZOL RNA isolation reagent (see catalog #732-6890 or #732-6880 to order separately).
Reagents
Reverse Transcription

Formulated for efcient reverse transcription
across a broad linear dynamic range

Potent RNase A inhibitors protect RNA during
setup and reverse transcription

Flexible input RNA capacity to suit different
experimental needs

Optimized for gene expression analysis using
real-time qPCR
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Reverse Transcription iScript
Kit Selector
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Select my
own primers
One-step
RT-qPCR with
SYBR

Green
One-step
RT-qPCR for
probes
iScript one-step
RT-PCR kit for probes
iScript
one-step
RT-PCR kit
with SYBR
Green
iScript Select
cDNA synthesis kit
1 g1 pg
total RNA
100 ng1 pg
total RNA
1 g1 pg
total RNA
2x probes RT-PCR
reaction mix (dNTPs,
iTaq DNA polymerase,
and stabilizers)
2x SYBR
Green
RT-PCR reaction mix
(dNTPs, iTaq
DNA
polymerase,
fuorescein,
SYBR
Green I dye,
and stabilizers)
5x iScript reaction mix
(dNTPs and
buffer components)
iScript reverse
transcriptase
(for one-step RT-PCR)
iScript reverse
transcriptase
(for one-step RT-PCR)
iScript reverse
transcriptase
Fast and
easy to use
iScript cDNA
synthesis kit
1 g100 fg
total RNA
5x iScript reaction mix
(dNTPs, oligo[dT],
random primers, and
buffer components)
iScript reverse
transcriptase
cDNA ready in 40 min
for qPCR
Maximize data
from single
20 l reaction
7.5 g100 fg
total RNA
5x iScript advanced
reaction mix
(dNTPs, oligo[dT],
random primers, and
buffer components)
iScript reverse
transcriptase
cDNA ready in 35 min
for qPCR
Forward and
reverse primers
and probe for
target gene
(not included)
RT-qPCR data
in 60 min
Forward and
reverse primers
for target gene
(not included)
RT-qPCR data in
6090 min
Oligo(dT),
random primers, and
gene-specifc primer
(GSP) enhancer
solution (3 vials)
cDNA ready in
4090 min for qPCR
Reduce
pipetting
variability
iScript reverse
transcription supermix
for RT-qPCR
1 g100 fg
total RNA
5x iScript
RT supermix
(dNTPs, oligo[dT],
random primers,
buffer components,
and iScript reverse
transcriptase)
cDNA ready in
40 min for qPCR
iScript advanced
cDNA synthesis kit
for RT-qPCR
iScript
Advanced cDNA Synthesis Kit for RT-qPCR

Increased qPCR data throughput and cost effectiveness from a single 20 l
reverse transcription (RT) reaction

Superior sensitivity and broad linear dynamic range for RT (7.5 g100 fg)

2-tube kit (5x iScript reaction mix and iScript reverse transcriptase) for ease
of use and reduced reaction setup time

Optimized blend of oligo(dT) and random primers ensures complete and
unbiased RNA sequence representation

RNase H+ MMLV reverse transcriptase (preblended with RNase inhibitor)
delivers high sensitivity for real-time RT-qPCR and eliminates additional
RNase H+ step

Potent blend of RNaseA inhibitor protects RNA during setup and RT

Short 35 min protocol allows fast qPCR data generation
Reagents
Two-Step Reverse Transcription Reagents
iScript Reverse Transcription Supermix
for RT-qPCR

1-tube format for simple and fast setup, and reduced pipetting variability

Liquid format at 20C offers superior stability and eliminates freeze/thaw cycle

Superior sensitivity and broad linear dynamic range for RT (1 g100 fg)


RNase H+ step


iScript reverse transcription supermix for RT-qPCR efciently
reverse transcribes RNA over a broad linear dynamic range
for reliable gene expression analysis data. Different amounts of
HeLa cell RNA (amounts shown in inset) were reverse transcribed
and one-tenth of the resulting cDNA was used as a template to
amplify -actin gene (~90 bp) in 20 l qPCR reactions with iQ

SYBR
Green supermix. Standard curve R

2
= 0.999, efciency =
99.7%, slope = 3.33. RFU, relative uorescence units.
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1 g
100 ng
10 ng
1 ng
100 pg
10 pg
1 pg
Excellent data reproducibility. PGK-1 mRNA (~160 bp), a gene that
encodes a glycolytic enzyme, was quantied using iScript reverse
transcription supermix for RT-qPCR both with 100 ng () and 100 pg ()
of input RNA. For each input RNA, 48 individual RT reactions were
performed and one-tenth of the resulting cDNA was used in the qPCR
reaction with SsoFast
probes supermix. The gene expression analysis

data show excellent reproducibility both with high and low levels of input
target mRNA. The ~10 Cq difference for the 1,000-fold dilution of RNA
(100 ng100 pg) demonstrates good reverse transcription efciencies
across different input RNAs. Cq, quantication cycle; RFU, relative
uorescence units.
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RNA Average Cq SD CV, %
100 ng 21.35 0.123 0.576
100 pg 31.56 0.147 0.465
www.bio-rad.com/
iscript
iScript advanced cDNA synthesis kit for RT-qPCR provides
superior sensitivity and a broad linear dynamic range for reverse
transcription. Total RNA (7.5 g1 pg) from HeLa cells was reverse
transcribed using the iScript advanced cDNA synthesis kit for RT-
qPCR in a 20 l reaction. A tenfold dilution of generated cDNA was
used as template to amplify -tubulin in a 10 l qPCR reaction with
iQ
SYBR
Green supermix on a CFX384
real-time PCR detection

system. Standard curve R
2
= 0.999, efciency = 90.7%, slope = 3.57.
Cq, quantication cycle; RFU, relative uorescence units.
35
30
25
20
15
C
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2 1 0 1 2 3 4
log starting quantity
103
102
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Reagents
Two-Step Reverse Transcription Reagents
iScript

cDNA Synthesis Kit

2-tube kit (5x iScript reaction mix and iScript reverse transcriptase) for
ease of use and reduced reaction setup time

Superior sensitivity and broad linear dynamic range for RT (1 g100 fg)


RNase H+ step


iScript Select cDNA Synthesis Kit

5-tube kit (random primers, oligo[dT], 5x iScript Select reaction mix, iScript
reverse transcriptase, and gene-specic primer enhancer solution)

Choice of priming strategy

Reliable synthesis of long cDNA >6 kb in length

Superior sensitivity and broad linear dynamic range for RT (1 g1 pg)
iScript reagents provide potent RNaseA inhibition. iScript
reagents for RT-qPCR include an optimum blend of RNaseA
inhibitor for protecting RNA integrity. Reverse transcription was
performed using 0.1 pg of input RNA with iScript reagent alone (),
spiked with RNaseA (), or spiked with RNaseA without the
RNaseA inhibitor included in the reaction (). 18S rRNA (~70 bp)
was amplied using iQ
SYBR
Green supermix. A signicant Cq

delay was observed when the reaction included RNaseA but no
RNaseA inhibitor, which demonstrates potent RNaseA inhibition.
RFU, relative uorescence units.
0 10 20 30 40
Cycles
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The iScript cDNA synthesis kit performs across a broad range
of concentrations. Input RNA (amounts shown in inset) was reverse
transcribed, and the resulting cDNA was amplified using iQ
SYBR

Green supermix. Standard curve r
2
= 0.998, efficiency = 96.5%.
RFU, relative fluorescence units.
0 5 10 15 20 25 30 35 40 45
Cycles
10
3
10
2
10
R
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1 g
100 ng
10 ng
1 ng
100 pg
10 pg
1 pg
iScript Select cDNA synthesis kit performs reliably over 6 orders
of magnitude using a gene-specific primer approach. Human
total RNA from 1 g to 1 pg was reverse transcribed using the iScript
Select cDNA synthesis kit. One-tenth of the resulting cDNA was
used as a template to amplify -actin gene with iQ
SYBR
Green
supermix. Standard curve r = 1.000, efficiency = 92.2%. RFU, relative
fluorescence units.
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iScript

One-Step RT-PCR Kit with SYBR
Green

For use on a broad range of real-time PCR instruments

Extremely sensitive detection (100 ng1 pg) of input RNA
iScript One-Step RT-PCR Kit for Probes

For use with all types of hybridization probes, including dual-labeled
oligonucleotide probes, FRET probes, and molecular beacons

Extremely sensitive detection (1 g1 pg) of input RNA
Benets of iScript one-step kits:

Provide powerful combination of iScript RNase H+ reverse transcriptase
and antibody-mediated hot-start iTaq
DNA polymerase

Are ideal for rapid, high-throughput gene expression analysis

Perform cDNA synthesis and qPCR in 1 tube, minimizing handling and
contamination risk
iScript
one-step RT-PCR kit with SYBR
Green provides high reproducibility and

sensitivity across a broad range of concentrations. Reactions were performed in triplicate,
along with no-template controls, using GAPDH primers and 100 ng100 fg total HeLa RNA.
Reactions were carried out on the iCycler iQ
real-time PCR detection system. Standard curve

r = 1.000, efficiency = 95%, slope = 3.47. RFU, relative fluorescence units.
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iScript one-step RT-PCR kit for probes delivers unparalleled results over an extremely
wide dynamic range. RNA (1 g100 fg) isolated from HeLa cells using the Aurum
total
RNA kit was reverse transcribed and amplified using primers to -actin and a FAM-labeled
detection probe. Each dilution was performed in triplicate and RT-PCR was carried out on the
iCycler iQ real-time PCR detection system. Standard curve r = 1.000, efficiency = 97.2%,
slope = 3.39. RFU, relative fluorescence units.
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Reagents
One-Step RT-qPCR Reagents
Catalog # Description $
Two-Step Reverse Transcription Reagents One-Step RT-qPCR Reagents
170-8842 iScript Advanced cDNA Synthesis Kit for RT-qPCR, 50 x 20 l reactions 170-8892 iScript One-Step RT-PCR Kit with SYBR Green, 50 x 50 l reactions
170-8843 iScript Advanced cDNA Synthesis Kit for RT-qPCR, 250 x 20 l reactions 170-8893 iScript One-Step RT-PCR Kit with SYBR Green, 200 x 50 l reactions
170-8890 iScript cDNA Synthesis Kit, 25 x 20 l reactions 170-8894 iScript One-Step RT-PCR Kit for Probes, 50 x 50 l reactions
170-8891 iScript cDNA Synthesis Kit, 100 x 20 l reactions 170-8895 iScript One-Step RT-PCR Kit for Probes, 200 x 50 l reactions
170-8840 iScript Reverse Transcription Supermix for RT-qPCR, 25 x 20 l reactions
170-8841 iScript Reverse Transcription Supermix for RT-qPCR, 100 x 20 l reactions
170-8896 iScript Select cDNA Synthesis Kit, 25 x 20 l reactions
170-8897 iScript Select cDNA Synthesis Kit, 100 x 20 l reactions
www.bio-rad.com/
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Reagents
Real-Time qPCR

Patented Sso7d fusion enzyme technology
delivers higher processivity and inhibitor
tolerance

Antibody-mediated hot-start technology
enables instant polymerase activation and
superior specicity

Choice of fast, standard, or universal
cycling conditions

Formulated for optimal performance on a
variety of real-time instruments
Reagents
Real-Time qPCR Supermixes
Property SsoAdvanced

Supermixes
iTaq
Universal
Supermixes
iQ
Supermixes Application-Specic
Kits and Reagents
Tolerance for PCR inhibitors

Sensitive detection of low-level target genes

High efciency even for difcult amplicons

Broad range of reaction conditions

Standard and fast cycling

Compatibility with any real-time instrument

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Reagents
Real-Time qPCR Supermixes
www.bio-rad.com/
supermixes
SsoAdvanced
Supermixes

Superior performance even from compromised samples

Tolerance for a broad range of reaction conditions and difcult amplicons

Optimal results from standard and fast PCR
iTaq
Universal Supermixes

Robust and sensitive qPCR data

Instant polymerase activation

Reliable results from standard and fast cycling conditions

Compatible with any real-time instrument
iQ
Supermixes

Reliable and reproducible qPCR performance

qPCR with increased specicity

Proven formulation for basic qPCR assays and needs

Quick activation of antibody-mediated hot-start enzyme
Application-Specic Kits and Reagents

Superior high resolution melt (HRM) data for single nucleotide
polymorphism (SNP) detection

Accurate methylation detection

Novel quantitative chromatin structure information using qPCR
Reagents
SsoAdvanced
Supermixes
SsoAdvanced
SYBR
Green Supermix*

Novel Sso7d fusion polymerase enables increased resistance to PCR
inhibitors and higher processivity for dye-based real-time qPCR

Robust formulation delivers maximum efciency, sensitivity, and
reproducibility across a broad range of standard and fast cycling conditions

Antibody-mediated hot-start technology and optimized buffer allow for instant
polymerase activation and rapid polymerization kinetics to enable fast PCR

Advanced formulation tolerates a broad range of reaction conditions,
primer concentrations, and temperature ranges
* SsoAdvanced
universal SYBR
Green supermix will be available soon and it will be compatible

with any real-time PCR instrument.
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SsoAdvanced
SYBR
Green supermix provides extreme

sensitivity in the detection of a single copy target gene.
The cyclin gene was amplied and detected from vefold serial
dilutions of 10 ng80 pg () and 3.2 pg () human genomic DNA.
Standard curve R
2
= 1 (for 10 ng80 pg), cyclin efciency = 103%.
Inset shows the standard curve for the various dilutions.
Cq, quantication cycle; RFU, relative uorescence units.
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10
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R
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Cycles
18 19 20 21 22
Cycles
R
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Exceptional reproducibility can be achieved with SsoAdvanced

SYBR
Green supermix. Efcient discrimination and reliable

quantication can be obtained from a 1.33-fold serial dilution of input
template. The GAPDH gene was amplied from varying amounts of
HeLa cDNA (1 ng136 pg). From left to right: 1 ng, 753 pg, 565 pg,
425 pg, 320 pg, 240 pg, 181 pg, and 136 pg. Standard curve R
2
=
0.999, GAPDH efciency = 96.2%. Inset is a magnied view showing
robust discrimination and reproducible amplication (six replicates for
each input amount). RFU, relative uorescence units.
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d
(R
F
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)/d
T
700
600
500
400
300
200
100
0
65 70 75 80 85 90 95
Temperature, C
SsoAdvanced
SYBR
Green supermix demonstrates superior

inhibitor tolerance. The ADAR gene was amplied from HeLa cDNA in the
presence of water alone, or in the presence of a known PCR inhibitor,
Eagles minimum essential medium (EMEM) with fetal bovine serum (FBS; 0,
2.5, 5, 10, and 20%), added to SsoAdvanced
SYBR
Green supermix ()
or a traditional Taq DNA polymerasebased qPCR master mix ().
SsoAdvanced
SYBR
Green supermix showed quality amplication

in all reactions (EMEM with 20% FBS data shown) while the Taq DNA
polymerasebased qPCR master mix failed to amplify in all EMEM with FBS
combinations (shown in the inset melt curve). RFU, relative uorescence units.
www.bio-rad.com/
supermixes
Sso7d Polymerase
The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, increases
processivity, and provides greater speed and reduced reaction times compared to
conventional DNA polymerases. Sso7d fusion polymerases are signicantly more resistant to
PCR inhibitors, making the SsoAdvanced and SsoFast
supermixes ideal choices for challenging

applications, such as direct qPCR, without the need for sample preparation.
Reagents
SsoAdvanced
Supermixes
SsoFast
Probes Supermix

Novel Sso7d fusion polymerase enables increased resistance to PCR
inhibitors and higher processivity for probe-based real-time qPCR

Robust formulation allows simultaneous detection of up to 2 different
gene targets using uorogenic probes with maximum efciency,
sensitivity, and reproducibility across a broad range of standard and fast
cycling conditions

Antibody-mediated hot-start technology and optimized buffer allow for instant
polymerase activation and rapid polymerization kinetics to enable fast PCR
www.bio-rad.com/
supermixes
R
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10
1
0 10 20 30 40
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C
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Exceptional reproducibility can be achieved on the CFX384

real-time PCR detection system with SsoFast probes supermix.
Efficient discrimination and reliable quantification can be obtained from
1.33-fold serial dilutions of input template. The GAPDH gene was
amplified from varying amounts of HeLa cDNA (1 ng102 pg). From
left to right: () 1 ng, 565 pg, 320 pg, 181 pg, and 102 pg;
() 752 pg, 425 pg, 240 pg, and 136 pg. GAPDH efficiency =
91.5%, R
2
= 0.997. Inset shows the standard curve for the various
dilutions. Total qPCR run time = 50 min. Cq, quantification cycle;
SsoFast probes supermix maintains exceptional stability on
the high-throughput CFX automation system. Tenfold serial
dilutions of 100 ng1 pg cDNA from human spleen were used in
each 20 l reaction to detect GAPDH. All reactions were assembled
and loaded onto the CFX automation system. The following cycling
conditions were used: 95C for 10 min, followed by 35 cycles
of 95C for 15 sec and 60C for 60 sec. After each plate run,
an additional hold time was introduced to prolong the total time
between plates. Results for five plates (048 hr) are shown.
SsoFast probes supermix delivers superior results for gene
expression analysis of two targets on the CFX96
real-time
PCR detection system, with no difference in detection of a low-
expressing gene in duplex or simplex. cDNA from human liver
(100 ng) was used in each 20 l reaction. () HEX-labeled GAPDH probe
duplex reaction; () Texas Redlabeled IL-2 probe duplex reaction;
() HEX-labeled GAPDH probe simplex reaction; () Texas Redlabeled
IL-2 probe simplex reaction. Total qPCR run time = 38 min. RFU, relative
fluorescence units.
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Plate # Start Time,
hr
PCR
Efficiency, %
R
2
1 0 97.0 0.999
2 14.0 97.6 0.999
3 24.5 95.9 0.999
4 39.5 97.3 0.999
5 48.0 95.9 0.999
Sso7d Polymerase
The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, increases
processivity, and provides greater speed and reduced reaction times compared to
conventional DNA polymerases. Sso7d fusion polymerases are signicantly more resistant to
PCR inhibitors, making the SsoAdvanced and SsoFast supermixes ideal choices for challenging
applications, such as direct qPCR, without the need for sample preparation.
Reagents
iTaq
iTaq
Universal SYBR
Green Supermix

Advanced 2x ready-to-use supermix, formulated to deliver robust qPCR
results with superior sensitivity, efciency, and specicity

Optimized buffer allows consistent results using both standard and fast
cycling protocols

Antibody-mediated iTaq DNA polymerase enables fast activation and
superior specicity in qPCR

Formulation developed for optimal results on any real-time PCR instrument
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Exceptional reproducibility can be achieved with iTaq

universal SYBR
Green supermix. Efcient discrimination and

reliable quantication can be obtained from a 1.33-fold serial
dilution of input template. The human -actin gene was amplied
from varying amounts of HeLa cDNA (1 ng136 pg). From left to
right: 1 ng, 753 pg, 565 pg, 425 pg, 320 pg, 240 pg, 181 pg, and
136 pg. Inset is a magnied view showing robust discrimination
and reproducible amplication (six replicates for each input
amount). RFU, relative uorescence units.
10
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3
10
2
R
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U
0 10 20 30 40
Cycles
iTaq
universal SYBR
Green supermix provides reliable gene

expression data. The human -actin gene was amplied from HeLa cDNA
(100 ng100 fg) using a CFX96
real-time PCR detection system. iTaq

universal SYBR
Green supermix produced greater than 90% efciency

over 6 orders of linear dynamic range. Standard curve R
2
= 0.999, -actin
efciency = 92.6%, slope = 3.51. RFU, relative uorescence units.
10
5
10
4
10
3
10
2
10
R
F
U
0 10 20 30 40
Cycles
iTaq
universal SYBR
Green supermix allows robust

amplication of genomic DNA. The human GAPDH gene was
amplied from human genomic DNA (50 ng5 pg) using a CFX96
real-time PCR detection system. iTaq
universal SYBR
Green
supermix produced a GAPDH efciency of 108.1% over several orders
of linear dynamic range. Standard curve R
2
= 0.993, slope = 3.14.
www.bio-rad.com/
supermixes
Reagents
iTaq
iTaq Universal Probes Supermix

Advanced 2x ready-to-use supermix, formulated to deliver robust qPCR
results with superior sensitivity, efciency, and specicity

Optimized buffer allows consistent results for simplex and duplex reactions
using both standard and fast cycling protocols

Antibody-mediated iTaq DNA polymerase enables fast activation and
superior specicity in qPCR

Formulation developed for optimal results on any real-time PCR instrument
iTaq universal probes supermix is excellent for gene expression
analysis. The human -actin gene was amplied from HeLa cDNA
(100 ng100 fg) using FAM-labeled probes on a CFX96
real-time PCR
detection system. iTaq universal probes supermix produced a -actin
efciency of 94.3% over 6 orders of linear dynamic range. Standard
curve R
2
= 0.999, slope = 3.47. RFU, relative uorescence units.
Exceptional reproducibility can be achieved with iTaq universal
probes supermix. Efcient discrimination and reliable quantication
can be obtained from a 1.33-fold serial dilution of input template. The
human -actin gene was amplied from varying amounts of HeLa
cDNA (1 ng136 pg). From left to right: 1 ng, 753 pg, 565 pg, 425 pg,
320 pg, 240 pg, 181 pg, and 136 pg. Inset is a magnied view showing
robust discrimination and reproducible amplication (six replicates for
each input amount). RFU, relative uorescence units.
iTaq universal probes supermix allows accurate detection of
low-abundance targets. The IL-1 gene was amplied from HeLa
cDNA (100, 10, 1, and 0.1 ng) using FAM-labeled probes on a CFX96
real-time PCR detection system. iTaq universal probes supermix
showed sensitive detection of the IL-1 gene even with very low cDNA
inputs. RFU, relative uorescence units.
10
4
10
3
10
2
R
F
U
0 10 20 30 40
Cycles
10
4
10
3
10
2
R
F
U
0 10 20 30 40
Cycles
10
4
10
3
10
2
R
F
U
0 10 20 30 40
Cycles
R
F
U
10
2
18 20 22 24 26
Cycles
www.bio-rad.com/
supermixes
Reagents
iQ
Supermixes
www.bio-rad.com/
supermixes
iQ
SYBR
Green supermix generates precise, quantitative results.

A vefold dilution series (50 ng80 pg) of human genomic DNA was amplied
using the supermix, primers, and a probe specic to the IL-1 gene. Triplicate
reactions at each concentration were amplied along with no-template
controls on the iCycler iQ
real-time PCR detection system. Standard curve

r = 0.999, efciency = 97.6%, slope = 3.38. RFU, relative fluorescence units.
10
3
10
2
0 5 10 15 20 25 30 35 40
Cycles
P
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iQ
SYBR
Green Supermix

Analysis of low-, medium-, and high-abundance target genes with superior
sensitivity and efciency

Formulated for maximum SYBR
Green I stability and performance in a

wide variety of real-time PCR instruments

Antibody-mediated hot-start polymerase for quick activation and
increased specicity
iQ
SYBR
Green supermix and the iScript
cDNA synthesis kit show

consistently high specicity over a broad dynamic range of cDNA.
Serial dilutions (1 g1 pg) of HeLa total RNA were reverse transcribed,
and the resulting cDNA was amplied using primers specic to the -actin
gene. Triplicate reactions at each concentration were amplied along with
no-template controls on the iCycler iQ real-time PCR detection system. The
consistent spacing of the curves reects accurate reverse transcription and
amplication. Standard curve r = 0.997, efciency = 89.1%, slope = 3.62.
10
4
10
3
0 5 10 15 20 25 30 35 40 44
Cycles
P
C
R

b
a
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lin
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-
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u
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d

c
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v
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f
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,

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Reagents
iQ
Supermixes
www.bio-rad.com/
supermixes
iQ Supermix

Maximum efciency and sensitivity for qPCR using uorogenic probes

Reliable amplication over a wide dynamic range of human genomic and
plasmid DNA concentrations

Contains antibody-mediated hot-start iTaq
DNA polymerase for quick

activation and increased specicity
10
3
10
2
0 5 10 15 20 25 30 35 40 45 50
Cycles
R
F
U
iQ supermix provides sensitive real-time detection over 8 orders
of magnitude. Tenfold dilutions of a plasmid containing 10
9
10 copies
of the -tubulin gene were amplified using iQ supermix and a FAM-
labeled hybridization probe for detection. Eight replicates at each
concentration were amplified along with no-template controls on the
MyiQ
real-time PCR detection system. Standard curve r = 0.999,

efficiency = 98.2%, slope = 3.36. RFU, relative fluorescence units.
10
3
10
2
0 5 10 15 20 25 30 35 40 45
Cycles
R
F
U
iQ multiplex powermix produces highly reliable qPCR results
for up to five targets in a single tube, with no difference in
detection of a low-expressing gene in multiplex or singleplex.
One-tenth of a 1 g cDNA synthesis reaction of human thymus total
RNA was used in each 20 l reaction. FAM-labeled -actin probe (),
Cy5-labeled -tubulin probe (), HEX-labeled GAPDH probe (),
TAMRA-labeled cyclophilin probe (), Texas Redlabeled IL-2 probe ().
iQ Multiplex Powermix

Robust supermix formulated for sensitive and efcient multiplex qPCR

Reliable quantication of up to 4 targets (expression levels can vary up
to 10
6
-fold between target genes) or up to 5 targets

Linearity over 6 orders of magnitude of input cDNA and 4 orders of
magnitude of input genomic DNA

Suitable for a wide variety of applications, including gene expression
analysis, SNP genotyping, SNP analysis, GMO detection, and viral
load detection
Precision Melt Supermix

Optimized formulation containing EvaGreen dye delivers robust PCR and
high resolution melt (HRM) performance

Sensitive and effective discrimination of all 4 SNP classes across a broad
range of amplicons

Accurate detection of CpG methylation status for epigenetic studies

Exceptional room temperature stability for high-throughput HRM studies

Reliable performance on any HRM-capable thermal cycler
Reagents
0.20
0.15
0.10
0.05
0.00
0.05
D
if
f
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c
e

R
F
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78 79 80 81 82 83
Temperature, C
A B
0.0
0.1
0.2
0.3
0.4
0.5
0.6
D
if
f
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r
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n
c
e

R
F
U
74 76 78 80 82 84
Temperature, C
0.02
0.00
0.02
0.04
0.06
0.08
D
if
f
e
r
e
n
c
e

R
F
U
75 76 77 78 79 80
Temperature, C
Precision melt supermix delivers robust HRM for SNPs. Discrimination of class I and IV SNP genotypes are shown in panels A
and B, respectively. Class I (A to G substitution) and class IV (A to T substitution) SNP genotypes from mouse genomic DNA were analyzed
using precision melt supermix. Wild type (), heterozygote (), and homozygous mutant () are shown in the difference plots normalized
to wild-type samples. HRM analysis was performed on a CFX384
real-time PCR detection system and genotypes were automatically

assigned by Precision Melt Analysis
software. Amplification was carried out for 35 cycles. Total run time including melt curve = 150 min.
Accurate methylation detection with precision melt supermix. Mixtures
of methylated and unmethylated human genomic DNA of varying ratios were
analyzed using HRM on a CFX384 real-time PCR detection system. Increasing
amounts of methylated DNA (, 0%; , 2%; , 5%; , 50%; , 75%; , 95%;
and , 100%) were analyzed for methylation of the human RARB2 gene. The
genomic region contains 7 CpG sites and is 88 base pairs in length. Total run
time including melt curve = 190 min. RFU, relative uorescence units.
www.bio-rad.com/
supermixes
R
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Reagents
www.bio-rad.com/
epiq
EpiQ
Chromatin Analysis Kit

Novel technique generates quantitative chromatin structure information
with strong correlation to gene expression levels

Quantitative assessment of chromatin structure of target genes in
cultured cells

Kit discriminates open, actively transcribed chromatin regions from closed,
transcriptionally silent regions

In situ chromatin digestion, genomic DNA purication, and real-time PCR all
combined in one workow

Short assay time assessment of chromatin structure can be
accomplished in less than 6 hr

Small sample requirement as little as 5 x 10
4
cells are required to
perform analysis
The EpiQ chromatin analysis kit utilizes nuclease accessibility to discriminate open vs. closed chromatin regions.
Amplication of proximal promoter regions for the epigenetically silenced HBB (reference) gene or the constitutively expressed
GAPDH (target) gene was carried out in HeLa cells using the EpiQ kit and EpiQ
chromatin SYBR
Green supermix on the

CFX96
real-time PCR detection system. A, closed chromatin regions were protected from nuclease digestion and remained
intact prior to amplication, resulting in minimal quantication cycle (Cq) delays (Cq = 0.58) following nuclease treatment; B, open
chromatin regions were susceptible to nuclease digestion and were unavailable for amplication, leading to signicant Cq delays
(Cq = 8.08) after nuclease treatment. A comparison of Cqs with the amplication efciencies for each gene target factored in
was used to determine the accessibility of the target gene, calculated to be >99% for GAPDH. RFU, relative uorescence units.
Chromatin consists of DNA spooled around complexes of histone
protein molecules called nucleosomes ().
R
F
U
10
4
10
3
10
2
10
1
0 10 20 30 40
Cycles
No nuclease
With nuclease
Cq target = 8.08
R
F
U
10
4
10
3
10
2
10
1
0 10 20 30 40
Cycles
No nuclease
With nuclease
Cq

ref = 0.58
A. HBB Reference Gene (epigenetically silenced) B. GAPDH Target Gene (constitutively expressed)
Open chromatin
Closed chromatin
EpiQ
Chromatin SYBR
Green Supermix

Robust formulation delivers superior sensitivity and efciency for qPCR
from genomic DNA templates

Protocol is optimized for difcult real-time qPCR reactions for high GC
amplicons

Supermix contains uorescein and ROX and is compatible with all
real-time PCR instruments except Applied Biosystems 7000, 7300,
7700, and 7900 models (additional ROX can be added by ordering
the dye separately, catalog #172-5858)
Reagents
Standard PCR Reagents
iTaq
DNA Polymerase

Antibody-mediated hot-start DNA polymerase for quick 3 min activation
at 95C

Polymerase prevents nonspecic amplication and primer-dimers in both
PCR and real-time PCR applications
dNTP Mix

Formulated for consistency and higher efciency in PCR and real-time PCR

Robust dNTP solution withstands multiple rounds of freeze-thawing and
temperature cycling
iProof
High-Fidelity DNA Polymerase

A high-delity DNA polymerase with 52-fold more accuracy than Taq
DNA polymerase

Unique Pyrococcus-like proofreading enzyme is fused to a dsDNA binding
protein, Sso7d

Long and fast PCR applications fragments up to 37 kb are amplied in
less time (1530 sec/kb) and with less enzyme (0.251 U/reaction)

Convenient 2x supermix is available for iProof polymerase and buffer for
GC-rich templates
iProof high-delity DNA polymerase demonstrates unrivaled speed, leading to dramatically
shorter overall reaction times. The reaction protocol for iProof polymerase was compared to the
recommended protocols for two competing polymerases. Each protocol was designed to amplify 1,
8, and 15 kb products in 30 cycles. Reactions with iProof polymerase used a two-step protocol with a
combined annealing and extension step, while the other reactions used three-step protocols with the
minimum recommended extension times. Overall reaction times include temperature ramping times.
iProof high-delity DNA polymerase amplies long templates with high yields. Left, various
fragments up to 37 kb in length were amplied from BAC DNA using a combined annealing/
extension step of 10 min per cycle and 30 U/ml of iProof polymerase. Right, various sequences up
to 28.8 kb were amplied directly from human genomic DNA using 30 U/ml of iProof polymerase in
GC buffer with a combined annealing/extension time of 10 min per cycle.
1kb
35 min
2 hr
2 hr
8kb
1.5 hr
9.5 hr
5.5 hr
15kb
2 hr 20 min
16.5 hr
(Failed amplification)
Total protocol time
T
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iProof high-fidelity DNA polymerase
Common high-fidelity polymerase
Taq polymerase
5
10
20 22 25 32
37
2.9
3.8
7.9
15
22 28 48 kb
23 kb
BAC DNA (37 kb) Human genomic DNA (28 kb)
www.bio-rad.com/
pcrreagents
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Reagents
Real-Time qPCR Reagents Selection Guide
SYBR
Green/ EvaGreen Supermixes Probes Supermixes One-Step Kits for RT-

qPCR
Real-Time PCR Instrument
SsoAdvanced

SYBR

Green
Supermix*
iTaq

Universal
SYBR
Green
Supermix
iQ
SYBR

Green
Supermix
SsoFast

EvaGreen

Supermix
EpiQ

Chromatin
SYBR
Green
Supermix
SsoFast
Probes
Supermix
iTaq
Universal
Probes
Supermix
iQ
Supermix
iQ
Multiplex
Powermix
iScript

One-Step
RT-PCR Kit
with SYBR

Green
iScript
One-Step
RT-PCR Kit
for Probes
Bio-Rad
CFX96
, CFX96 Touch
,
CFX384
, CFX384 Touch
,
CFX Connect

iQ
, iQ
5, MyiQ
, MyiQ
2

MiniOpticon
, DNA Engine
Opticon
I and II

Applied Biosystems
StepOne/StepOne Plus

7500, ViiA 7

7000, 7300, 7700, 7900HT

Stratagene
Mx3000P, 3005P, 4000

Eppendorf
Mastercycler ep
realplex 2 or 4

QIAGEN/Corbett
Rotor-Gene 3000, 6000, Q

Roche
LightCycler 480

LightCycler 1.0, 1.5, 2.0

Idaho Technology
LightScanner HR-1

LightScanner 32

Recommended for use as is ROX reference setting must be turned off BSA must be added according to instrument specications
* SsoAdvanced
universal SYBR
Green supermix will be available soon.

Reagents
SsoAdvanced Supermixes
172-5260 SsoAdvanced SYBR Green Supermix, 2 ml (2 x 1 ml vials), 200 x 20 l reactions
172-5261 SsoAdvanced SYBR Green Supermix, 5 ml (5 x 1 ml vials), 500 x 20 l reactions
172-5262 SsoAdvanced SYBR Green Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 l reactions
172-5230 SsoFast Probes Supermix, 2 ml (2 x 1 ml vials), 200 x 20 l reactions
172-5231 SsoFast Probes Supermix, 5 ml (5 x 1 ml vials), 500 x 20 l reactions
172-5232 SsoFast Probes Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 l reactions
172-5233 SsoFast Probes Supermix, 20 ml (20 ml bottle), 2,000 x 20 l reactions
iTaq Universal Supermixes
172-5120 iTaq Universal SYBR Green Supermix, 2 ml (2 x 1 ml vials), 200 x 20 l reactions
172-5121 iTaq Universal SYBR Green Supermix, 5 ml (5 x 1 ml vials), 500 x 20 l reactions
172-5122 iTaq Universal SYBR Green Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 l reactions
172-5130 iTaq Universal Probes Supermix, 2 ml (2 x 1 ml vials), 200 x 20 l reactions
172-5131 iTaq Universal Probes Supermix, 5 ml (5 x 1 ml vials), 500 x 20 l reactions
172-5132 iTaq Universal Probes Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 l reactions
iQ Supermixes
170-8880 iQ SYBR Green Supermix, 2.5 ml (2 x 1.25 ml vials), 100 x 50 l reactions
170-8882 iQ SYBR Green Supermix, 12.5 ml (10 x 1.25 ml vials), 500 x 50 l reactions
170-8884 iQ SYBR Green Supermix, 25 ml (20 x 1.25 ml vials), 1,000 x 50 l reactions
170-8885 iQ SYBR Green Supermix, 50 ml (50 ml bottle), 2,000 x 50 l reactions
170-8886 iQ SYBR Green Supermix, 25 ml (5 x 5 ml vials), 1,000 x 50 l reactions
170-8887 iQ SYBR Green Supermix, 50 ml (10 x 5 ml vials), 2,000 x 50 l reactions
170-8860 iQ Supermix, 2.5 ml (2 x 1.25 ml vials), 100 x 50 l reactions
170-8862 iQ Supermix, 12.5 ml (10 x 1.25 ml vials), 500 x 50 l reactions
170-8864 iQ Supermix, 25 ml (20 x 1.25 ml vials), 1,000 x 50 l reactions
172-5848 iQ Multiplex Powermix, 1.25 ml (1 x 1.25 ml vial), 50 x 50 l reactions
172-5849 iQ Multiplex Powermix, 5 ml (4 x 1.25 ml vials), 200 x 50 l reactions
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Application-Specific Kits and Reagents
172-5110 Precision Melt Supermix, 2 ml (2 x 1 ml vials), 200 x 20 l reactions
172-5112 Precision Melt Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 l reactions
172-5400 EpiQ Chromatin Analysis Kit, 50 preparations
172-5401 EpiQ Chromatin Analysis Kit, 100 preparations
172-5404 EpiQ Chromatin SYBR Green Supermix, 5 ml (5 x 1 ml vials), 500 x 20 l reactions
172-5405 EpiQ Chromatin SYBR Green Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 l reactions
Standard PCR Reagents
170-8870 iTaq DNA Polymerase, 250 U, 5 U/l
170-8875 iTaq DNA Polymerase, 5,000 U, 5 U/l
170-8874 dNTP Mix, 200 l
172-5300 iProof High-Fidelity DNA Polymerase, 20 U, 2 U/l
172-5310 iProof HF Master Mix, 0.04 U/l, 100 x 50 l reactions
172-5311 iProof HF Master Mix, 0.04 U/l, 500 x 50 l reactions
172-5320 iProof GC Master Mix, 0.04 U/l, 100 x 50 l reactions
172-5321 iProof GC Master Mix, 0.04 U/l, 500 x 50 l reactions
172-5858 ROX Passive Reference Dye, 0.5 ml
Additional Real-Time qPCR Supermixes*
172-5200 SsoFast EvaGreen Supermix, 2 ml (2 x 1 ml vials), 200 x 20 l reactions
172-5201 SsoFast EvaGreen Supermix, 5 ml (5 x 1 ml vials), 500 x 20 l reactions
172-5202 SsoFast EvaGreen Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 l reactions
172-5203 SsoFast EvaGreen Supermix, 20 ml (20 ml bottle), 2,000 x 20 l reactions
* There are additional supermixes available. For more information, go to www.biorad.com/supermixes.
Reagents

Precisely manufactured for optimal t and
cycling performance

Produced in Class 10,000 or 100,000 cleanroom
environment

Certied to be free of DNase, RNase, and human
genomic DNA

Extremely uniform wells reduce well-to-well
variability in real-time PCR

Warp-free Hard-Shell
plates are designed for

optimum performance with automation
P
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Instrument Compatibility
0.2 ml Tubes 384-Well Plates
Individual High-Prole Strips High-Prole Strips Low-Prole Hard-Shell
Standard Hard-Shell 480

Catalog # TBI-0201, TFI-0201, TWI-0201 TBS-xxxx, TBC-xxxx TLS-xxxx HSP-3xxx HSR-48xx
Thermal Cycler
Bio-Rad C1000
, C1000 Touch
, S1000
Bio-Rad DNA Engine
,
DNA Engine Tetrad
,
DNA Engine Tetrad 2,
DNA Engine Dyad
,
Dyad Disciple
, PTC-100
Bio-Rad T100
, MyCycler

Bio-Rad iCycler

Bio-Rad MJ Mini

Applied Biosystems 0.2 ml tube

cyclers (2720, 9700, Veriti)
Applied Biosystems 0.1 ml tube

cyclers (9800 fast, Veriti fast)
Applied Biosystems 384-well

cyclers (9700, Veriti)
Eppendorf Mastercycler series
Bio-Rad CFX96
, CFX96 Touch
, CFX384
,*

CFX384 Touch
,* CFX Connect
Bio-Rad iCycler iQ
, iQ
5, MyiQ
, MyiQ
2
Bio-Rad Chromo4

Bio-Rad DNA Engine Opticon
and Opticon 2
Bio-Rad MiniOpticon
*
Applied Biosystems standard

systems (7300, 7500, 7900HT)
Applied Biosystems fast systems
(7500 fast, 7900HT fast, StepOne,
StepOnePlus)
Eppendorf Mastercycler ep realplex
Stratagene (Agilent) Mx series
QIAGEN/Corbett Rotor-Gene
Roche LightCycler 480
Other Instruments
Applied Biosystems DNA

sequencers (3100, 3700, 3730)
Idaho Technology LightScanner
Recommended
Compatible
48- and 96-Well Plates
Hard-Shell
Semi-Skirted Hard-Shell Skirted Multiplate
Unskirted Multiplate Unskirted iQ
Semi-Skirted
High-Prole Low-Prole High-Prole Low-Prole High-Prole
Catalog # HSS-xxxx HSP-9xxx MLP-xxxx MLL-xxxx 223-9441
Thermal Cycler
Bio-Rad C1000
, C1000 Touch
,

S1000
Bio-Rad T100

Bio-Rad DNA Engine
,
DNA Engine Tetrad
,
DNA Engine Tetrad 2,
DNA Engine Dyad
,
Dyad Disciple
, PTC-100
Bio-Rad MyCycler

Bio-Rad iCycler

Bio-Rad MJ Mini

Applied Biosystems
0.2 ml tube cyclers

(2720, 9700, Veriti)
Applied Biosystems
0.1 ml tube cyclers
(9800 fast, Veriti fast)
Eppendorf Mastercycler series

Bio-Rad CFX96
, CFX96 Touch
,

CFX Connect

Bio-Rad iCycler iQ
, iQ
5, MyiQ
,

MyiQ
2
Bio-Rad Chromo4

Bio-Rad DNA Engine

Opticon
, Opticon 2
Bio-Rad MiniOpticon
*
Applied Biosystems standard

systems (7300, 7500, 7900HT) Except 7900HT Except 7900HT
Applied Biosystems fast systems
(7500 fast, StepOne, StepOnePlus) Except 7900 fast
Eppendorf Mastercycler ep realplex

Stratagene (Agilent) Mx series
Other Instruments
Applied Biosystems DNA

sequencers (3100, 3700, 3730)

Recommended
Compatible
* CFX384
, CFX384 Touch
, and MiniOpticon real-time PCR detection systems are factory calibrated for white tubes and white-well plates. White plastics are recommended due to their superior
signal-to-noise ratio. Using clear tubes or clear-well plates on these instruments will require user calibration.
Instrument Compatibility
P
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TLS-0801, clear
TLS-0851, white
Low-Prole Tube Strips without Caps
TBS-0201, 8-tube, clear
TBS-1201, 12-tube, clear
High-Prole Tube Strips without Caps
Individual PCR Tubes, 0.2 and 0.5 ml
These high-prole PCR tubes have double-locking caps that wont pop open
during cycling. PCR volume ranges are 5125 l for 0.2 ml tubes and 10200 l
for 0.5 ml tubes. Tubes with at, frosted caps for easy labeling are available in
both 0.2 and 0.5 ml sizes (not suitable for real-time PCR).
To help prevent accidental contamination by multiple users, the 0.5 ml individual
tubes with attached caps are available in resealable plastic bags of 100 tubes.
Use of a capping tool is recommended for proper sealing of caps on tubes.
Both tubes and caps are available in strips of 8 or 12 for use in 48-well and
96-well sample blocks.

Tight sealing and convenient handling for multiple samples

Choice of domed or at optical cap strips
TWI-0201,
0.2 ml, domed cap
TBI-0501, TBI-0502,
0.5 ml, at cap
TFI-0201,
0.2 ml, at cap
TBI-0201,
0.2 ml, without cap
High-Prole 0.2 ml PCR Tube Strips
Recommended reaction volumes are 5125 l, which are suitable for most
standard PCR and qPCR instruments.
Low-Prole 0.2 ml PCR Tube Strips
These tubes reduce the potential for condensate formation and also allow
greater light capture in uorescence assays, such as those performed in
real-time PCR. Low-prole tubes are ideal for use in fast and low-volume
PCR reactions. The tube height is 15.5 mm. Low-prole tubes are available in
opaque white for optical applications.
Cap Strips for 0.2 ml PCR Tubes and PCR Plates
These cap strips provide extremely tight sealing of all Bio-Rad 0.2 ml
PCR tubes and plates during thermal cycling and cold storage. Flat cap
strips feature ultraclear upper surfaces, which are ideal for uorescence
applications. Average light transmittance is 1.7-fold higher than with standard-
clarity domed cap strips. Flat caps are available in strips of 8 and domed
caps are available in strips of 8 or 12. Use of a capping tool is recommended
for proper sealing of caps on tubes or plates.
TCS-0803, ultraclear
Optical Flat 8-Cap Strips
TCS-0801, 8-cap, clear
TCS-1201, 12-cap, clear
Domed Cap Strips
Tubes and Strips
Individual Tubes
www.bio-rad.com/
pcrplastics
Capping Tools and Racks Multiplate 48-Well PCR Plates
The versatile, unskirted design and 48-well format make these Multiplate
unskirted PCR plates ideal for laboratories using 48-well blocks on Bio-Rad
instruments. The plates are suitable for reaction volumes of 5125 l. The
polypropylene construction of Multiplate PCR plates confers very low protein
binding and excellent preservation of sample volume. When less than a full
plate is needed, these plates can be easily cut with scissors to the required
size. Two plate styles are available:

High-prole (20.70 mm) wells, clear designed to t in most thermal
cyclers

Low-prole (15.50 mm) wells, clear or white optimized for fast PCR and
low-volume reactions
Compatible Instruments
Bio-Rad C1000
, C1000 Touch
, T100
(high-prole plates only), S1000
, DNA Engine
family,
MJ Mini
, MiniOpticon
(low-profile white plates recommended)

MLP-4801, clear
Multiplate 48-Well Unskirted PCR Plates
MLL-4801, clear
MLL-4851, white
Multiplate Low-Prole 48-Well Unskirted PCR Plates
TRC-9601, ANSI/SBS standard
PCR Tube Rack
ECT-1000
Easy Cap
Tool
ECT-2000
Strip Cap Tool
TRC-0501, with covers
96-Place Racks
Capping Tools, Racks, and Multiplate

Plates
www.bio-rad.com/
pcrplastics
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Features of Hard-Shell PCR plates include:

Uniform wells that reduce well-to-well variability in optical assays, such as
those performed in real-time PCR

White-well option, which allows increased uorescent signal strength

Color-coded skirts with clear or white wells

Low-cost, user-readable bar code option for convenient database tracking
Hard-Shell Low-Prole 96-Well Skirted PCR Plates
Features of the skirted plates include:

Reaction volumes of 5125 l (200 l maximum)

Low-prole (16.05 mm) wells optimized for low-volume reactions and fast PCR

Superior stability and atness, allowing precise positioning for automation

Full skirt for robotic handling and labeling surface

Footprint and well spacing that match ANSI/SBS standard dimensions

Black alphanumeric labeling for easy well identication
Bio-Rad C1000
, C1000 Touch
, S1000
, DNA Engine
family, PTC-100
, CFX96
,
CFX96 Touch
, CFX Connect
, Chromo4
, DNA Engine Opticon
; Eppendorf Mastercycler series;

Hard-Shell Technology
Hard-Shell PCR plates are specically designed to withstand the stresses
of heat sealing, thermal cycling, and robotic handling. The patented two-
component design features a skirt and deck molded from a rigid, thermostable
polymer. In a separate step, the thin-wall wells are molded of virgin
polypropylene selected for low DNA binding. This design prevents problems
due to the warping, shrinkage, and sticking that may occur when single-
component polypropylene PCR plates are exposed to the high temperatures
of thermal cycling or heat sealing. Thus, performance is improved in many
applications. In addition, these plates can withstand 80C storage and high
centrifugation forces, making them convenient for alcohol precipitations.
HSP-9601, white shell, clear well
HSP-9655, white shell, white well
Other colors and bar code option available
Hard-Shell Low-Prole 96-Well Skirted PCR Plates
The skirt and deck of a
Hard-Shell plate prevent
warping and shrinkage
Thin-wall polypropylene V-shaped wells
enable optimal thermal transfer and
recovery of low-volume samples
Raised rims allow for
tight sealing
Hard-Shell
Plates
www.bio-rad.com/
pcrplastics
Hard-Shell High-Prole 96-Well Semi-Skirted
PCR Plates
These Hard-Shell PCR plates t most thermal cyclers. Features of semi-
skirted plates include:


High-prole (20.75 mm) wells that t most thermal cyclers, real-time PCR
detection systems, and DNA sequencers

Warp-free half-height skirt for improved robotic handling and labeling surface

Black alphanumeric labeling for easy well identication
Bio-Rad C1000
, C1000 Touch
, S1000
, T100
, DNA Engine
family, PTC-100
, iCycler
, iQ
5,
iCycler iQ
, MyiQ
, MyiQ
2, Chromo4
; Applied Biosystems 0.2 ml tube cyclers, real-time

systems, and DNA sequencers; Eppendorf Mastercycler series; Stratagene (Agilent) Mx series
The single-component polypropylene construction of Multiplate PCR plates
confers very low protein binding and excellent retention of sample. When less
than a full plate is needed, these plates are easily cut with scissors to the required
size. Two plate styles are available.
High-prole for most standard instruments
HSS-9601, clear shell, clear well
HSS-9641, green shell, clear well
HSS-9901, clear shell, clear well, bar-coded
HSS-9665, black shell, white well
Hard-Shell High-Prole 96-Well Semi-Skirted PCR Plates
MLP-9601, clear
MLP-9651, white
MLL-9601, clear
MLL-9651, white
Multiplate Low-Prole 96-Well Unskirted PCR Plates
Low-prole for most fast instruments
Bio-Rad C1000, C1000 Touch, S1000, DNA
Engine family, PTC-100, MJ Mini, CFX96
,
CFX96 Touch
, CFX Connect
, Chromo4, DNA
Engine Opticon
; Applied Biosystems 0.1 ml

tube fast cyclers and real-time systems;
Eppendorf Mastercycler series; Idaho
Technology LightScanner
Bio-Rad C1000, C1000 Touch, S1000, T100,
DNA Engine family, PTC-100, MyCycler
,
iCycler, MJ Mini
, iQ5, iCycler iQ, MyiQ, MyiQ2,

Chromo4, MiniOpticon
; Applied Biosystems
0.2 ml tube cyclers, real-time systems, and
DNA sequencers; Eppendorf Mastercycler
series; Stratagene (Agilent) Mx series; Idaho
Technology LightScanner
Hard-Shell
and Multiplate

Plates
www.bio-rad.com/
pcrplastics
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Hard-Shell 384-Well PCR Plates
Hard-Shell PCR plates are designed to withstand the stresses of thermal
cycling and robotic handling. The patented two-component design provides
superior stability and atness, allowing precise positioning for automation.
Features include:


Extremely uniform wells that reduce well-to-well variability in optical assays,
such as those performed in real-time PCR

White-well option for increased uorescent signal strength

Color-coded skirts with clear or white wells

Warp-free skirt and deck for improved robotic handling

Footprint and well spacing that match ANSI/SBS standard dimensions

Low-cost, user-readable bar code option for convenient database tracking
Two plate styles are available.
Standard for most instruments
480 for Roche LightCycler 480
Roche LightCycler 480; Bio-Rad C1000,
C1000 Touch, S1000, DNA Engine family,
CFX384, CFX384 Touch; Applied Biosystems
cyclers and real-time systems; Eppendorf
Mastercycler series
iQ High-Prole 96-Well Semi-Skirted
Real-Time PCR Plates
These semi-skirted, high-prole PCR plates are optimized for iQ
5, iCycler iQ
,
MyiQ
2, and MyiQ
real-time PCR detection systems. The semi-skirted

design adds stiffness and a labeling surface. Plates are perforated every
three columns for easy setup of triplicate reactions.
Bio-Rad C1000
, C1000 Touch
, S1000
,

T100
, DNA Engine
family, PTC-100
, MyCycler
,
iCycler
, MyiQ, iCycler iQ, iQ5, MyiQ2, Chromo4
; Applied Biosystems 0.2 ml tube cyclers and

real-time systems; Eppendorf Mastercycler series; Stratagene (Agilent) Mx series
223-9441
iQ 96-Well PCR Plates
HSP-3801, clear shell, clear well
HSP-3805, clear shell, white well
Other colors and bar code option available
Hard-Shell 384-Well Standard PCR Plates
HSR-4805K, clear shell, white well, 100 plates and 100 seals
HSR-4801K, clear shell, clear well, 100 plates and 100 seals
Hard-Shell 384-Well 480 PCR Plates
Bio-Rad C1000, C1000 Touch, S1000, DNA
Engine family, CFX384
, CFX384 Touch
;
Applied Biosystems cyclers, real-time systems,
and DNA sequencers; Eppendorf Mastercycler
series; Idaho Technology LightScanner
IQ
and Hard-Shell
Plates
www.bio-rad.com/
pcrplastics
Microseal 'C' Optical Seals
Microseal 'C' optical seals are optically clear adhesive lms designed for
optical assays, such as those performed in real-time PCR. The seals can be
used for microplates with raised well rims. Features include:

Form a tight seal for PCR and qPCR

Pressure- and heat-sensitive adhesive sticks to the plate, not your gloves

Sealing tool for applying even pressure is included

Free from DNase, RNase, and human DNA contaminants
Microseal 'B' Adhesive Seals, Optically Clear
Microseal 'B' seals provide an adhesive-based sealing option for thermal
cycling using thin-wall PCR plates. The strong adhesive layer ensures secure
sample storage (from as low as 40C and up to 110C) or transport before or
after cycling, as well as tight sealing during thermal cycling when supplemental
pressure is applied by a heated lid. This clear polyester lm allows easy
inspection of sample wells and effective light transmission for optical assays.
The sealing surface is protected from contamination by a peel-away release
liner. Perforated end-tabs allow removal of overhanging lm for automation and
other applications. Accessories include:

Optical compression pad (96-well) enhances the seal integrity of
Microseal 'B' clear seals when used in real-time PCR detection systems
Optical lm sealing kit contains 100 Microseal 'B' clear seals and an
optical compression pad
MSB-1001
Microseal 'B' Adhesive Seals
MSA-5001
Microseal 'A' Film
MSR-0001
Sealing Roller
ADR-3296
Optical Compression Pad
Microseal 'F' Foil
These aluminized foil seals act as a barrier against evaporation from 80C to
105C. They are thin enough to pierce with a pipet tip for recovery of samples
from individual wells, and they are suitable for use with automated systems,
such as the ABI 3700 DNA analyzer. In addition to cold storage applications,
they can be used for thermal cycling for sample volumes of 25 l (96-well) or
5 l (384-well).
Microseal 'A' Film
Microseal 'A' lm quickly and effectively seals the full range of Bio-Rad PCR
plates and tubes. The pliant inner layer is designed to seal tightly during cycling
yet to release smoothly to minimize the risk of aerosol formation and cross-
contamination of samples. This lm is easily cut for use with fewer than 96
wells; a peel-away release liner protects the sealing surface from contamination
(not suitable for qPCR). An optional sealing roller provides a quick way to
rmly seal Microseal 'A' lm on an entire array of wells.
MSC-1001
Microseal 'C' Optical Seals
Microseal
Sealers
MSF-1001
Microseal 'F' Foil
www.bio-rad.com/
pcrplastics
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Catalog # Description
Individual PCR Tubes with Attached Caps (0.2 ml)
TFI-0201 PCR Tubes with Flat Caps (0.2 ml), clear, 1,000
TWI-0201 PCR Tubes with Domed Caps (0.2 ml), clear, 1,000
Individual PCR Tubes without Caps (0.2 ml)
TBI-0201 PCR Tubes without Caps (0.2 ml), clear, 1,000
Individual PCR Tubes with Attached Caps (0.5 ml)
TBI-0501 PCR Tubes with Flat Caps (0.5 ml), clear, 1,000 (2 bags of 500)
TBI-0502 PCR Tubes with Flat Caps (0.5 ml), clear, 800 (8 bags of 100)
High-Profile Tube Strips without Caps (0.2 ml)
TBS-0201 8-Tube Strips without Caps (0.2 ml), clear, 120 strips (960 PCR tubes)
TBS-1201 12-Tube Strips without Caps (0.2 ml), clear, 100 strips (1,200 PCR tubes)
Low-Profile 8-Tube Strips without Caps (0.2 ml)
TLS-0801 Low-Profile 8-Tube Strips without Caps (0.2 ml), clear, 120 (960 PCR tubes)
TLS-0851 Low-Profile 8-Tube Strips without Caps (0.2 ml), white, 120 (960 PCR tubes)
Domed Cap Strips
TCS-0801 Domed 8-Cap Strips, for 0.2 ml PCR tubes and plates, clear, 120
TCS-1201 Domed 12-Cap Strips, for 0.2 ml PCR tubes and plates, clear, 200
Optical Flat Cap Strips
TCS-0803 Optical Flat 8-Cap Strips, for 0.2 ml PCR tubes and plates, ultraclear, 120
High-Profile Tube Strips with Domed Cap Strips (0.2 ml)
TBC-0802 8-Tube Strips and Domed Cap Strips (0.2 ml), clear, 20 bags of 12 x 8-tube strips and 12 x 8-cap strips (1,920 PCR tubes and 1,920 caps)
TBC-1202 12-Tube Strips and Domed Cap Strips (0.2 ml), clear, 20 bags of 8 x 12-tube strips and 8 x 12-cap strips (1,920 PCR tubes and 1,920 caps)
Capping Tools and Racks
TRC-9601 PCR Tube Rack, ANSI/SBS standard, white, 10
TRC-0501 96-Place Racks, with covers, for PCR tubes and unskirted and semi-skirted microplates, assorted colors, 5
ECT-1000 Easy Cap Tool, ensures tight seal for 0.2 ml PCR tubes or 96-well microplates
ECT-2000 Strip Cap Tool, for sealing 8- and 12-cap strips on PCR plates or tubes
Multiplate 48-Well PCR Plates
MLP-4801 Multiplate 48-Well Unskirted PCR Plates, clear, 50 plates
MLL-4801 Multiplate Low-Profile 48-Well Unskirted PCR Plates, clear, 50 plates
MLL-4851 Multiplate Low-Profile 48-Well Unskirted PCR Plates, white, 50 plates
MLP-9601 Multiplate 96-Well Unskirted PCR Plates, clear, 25 plates
MLP-9651 Multiplate 96-Well Unskirted PCR Plates, white, 25 plates
MLP-9631 Multiplate 96-Well Unskirted PCR Plates, blue, 25 plates
Multiplate Low-Profile 96-Well Unskirted PCR Plates
MLL-9601 Multiplate Low-Profile 96-Well Unskirted PCR Plates, clear, 25 plates
MLL-9651 Multiplate Low-Profile 96-Well Unskirted PCR Plates, white, 25 plates
iQ 96-Well PCR Plates
223-9441 iQ 96-Well PCR Plates, 25 plates
Description Clear Wells White Wells Black Wells
Hard-Shell Low-Profile 96-Well Skirted PCR Plates
White shell, 50 HSP-9601 HSP-9655
Red shell, 50 HSP-9611
Yellow shell, 50 HSP-9621
Blue shell, 50 HSP-9631 HSP-9635
Green shell, 50 HSP-9641 HSP-9645
Black shell, 50 HSP-9661 HSP-9665 HSP-9666
White shell, bar-coded, 50 HSP-9901 HSP-9955
Hard-Shell High-Profile 96-Well Semi-Skirted PCR Plates
Clear shell, 25 HSS-9601
Green shell, 25 HSS-9641
Black shell, 25 HSS-9665
Clear shell, bar-coded, 25 HSS-9901
Hard-Shell 384-Well Standard PCR Plates
Clear shell, 50 HSP-3801 HSP-3805
Red shell, 50 HSP-3811
Yellow shell, 50 HSP-3821
Blue shell, 50 HSP-3831
Green shell, 50 HSP-3841
Black shell, 50 HSP-3865 HSP-3866
Clear shell, bar-coded, 50 HSP-3901 HSP-3905
50 Plates 100 Plates, 100 Microseal 'C' Seals
Hard-Shell 384-Well 480 PCR Plates with Bar Code on Row A Side
Clear shell, white well HSR-4805 HSR-4805K
Clear shell, clear well HSR-4801 HSR-4801K
Catalog # Description
PCR Plate Sealers
MSA-5001 Microseal 'A' Film, package of 50 seals
MSB-1001 Microseal 'B' Adhesive Seals, optically clear, 100 seals
MSC-1001 Microseal 'C' Optical Seals, 100 seals
MSF-1001 Microseal 'F' Foil, package of 100 seals
MSR-0001 Sealing Roller, for film seals
ADR-3296 Optical Compression Pad, for improved film sealing of 96-well plates in DNA Engine Opticon 2 and Chromo4 systems
ADR-5001 Pressure Pad, uniformly distributes lid pressure for sealing film
MSO-1001 Optical Film Sealing Kit, for 96-well plates, includes optical compression pad, 100 Microseal 'B' clear adhesive seals
223-9444 Optical Sealing Tape, package of 100 sheets
223-9442 96-Well PCR Plate Sealing Mats, 5
Life Science
Group
11-1817 0312 Sig 1211 Bulletin 6090 Rev C US/EG
Bio-Rad
Laboratories, Inc.
Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 11 5044 5699
Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00
France 01 47 95 69 65 Germany 089 31 884 0 Greece 30 210 9532 220 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300
Israel 03 963 6050 Italy 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 0318 540666
New Zealand 64 9 415 2280 Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 14 04
Singapore 65 6415 3188 South Africa 27 861 246 723 Spain 34 91 590 5200 Sweden 08 555 12700 Switzerland 061 717 95 55
Taiwan 886 2 2578 7189 Thailand 800 88 22 88 United Kingdom 020 8328 2000
Cy is a trademark of GE Healthcare group companies. Eppendorf and Mastercycler are trademarks of Eppendorf AG. EvaGreen is a trademark of Biotium, Inc. Bio-Rad Laboratories, Inc. is
licensed by Biotium, Inc. to sell reagents containing EvaGreen dye for use in real-time PCR, for research purposes only. FAM, ROX, StepOne, StepOnePlus, and Veriti are trademarks of Applera
Corporation. HRM and Rotor-Gene are trademarks of QIAGEN GmbH. LightCycler is a trademark of Roche Diagnostics GmbH. LightScanner is a trademark of Idaho Technology Inc. Mx,
Mx3000P, Mx3005P, and Mx4000 are trademarks of Stratagene Corporation. SYBR is a trademark of Molecular Probes, Inc. Bio-Rad Laboratories, Inc. is licensed by Molecular Probes, Inc. to sell
reagents containing SYBR Green I for use in real-time PCR, for research purposes only. Texas Red is a trademark of Invitrogen Corporation. ViiA is a trademark of Life Technologies Corporation.
Notice regarding Bio-Rad thermal cyclers and real-time systems:
Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchasers own internal research and development and for use in human in vitro diagnostics and all other
applied elds under U.S. Patent Number 5,475,610 (Claims 1, 44, 158, 160163, and 167 only), or corresponding claims in its non-U.S. counterpart, owned by Applera Corporation. No right is conveyed
expressly, by implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5' nuclease methods. Further information on purchasing licenses
may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Bio-Rads real-time thermal cyclers are licensed real-time thermal cyclers under Appleras U.S. Patent Number 6,814,934 B1 for use in research, human in vitro diagnostics, and all other elds except
veterinary diagnostics.
Bio-Rads thermal cyclers and real-time thermal cyclers are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 6,767,512
and 7,074,367.
Practice of the patented 5' Nuclease Process requires a license from Applied Biosystems. The purchase of these products includes an immunity from suit under patents specied in the product insert
to use only the amount purchased for the purchasers own internal research when used with the separate purchase of Licensed Probe. No other patent rights are conveyed expressly, by implication, or
by estoppel. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Hard-Shell plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.

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Amplication Reagents and Plastics

Total RNA Fatty and Fibrous Tissue Kit

efciently lyses cells and tissues, deproteinates RNA, and

real-time PCR detection system. RFU, relative

RT-qPCR Sample Preparation Reagent

RNA Isolation Reagent

Total RNA Kits PureZOL

Advanced cDNA Synthesis Kit for RT-qPCR

Green supermix. Standard curve R

probes supermix. The gene expression analysis

Green supermix on a CFX384

real-time PCR detection

Green supermix. A signicant Cq

one-step RT-PCR kit with SYBR

Green provides high reproducibility and

real-time PCR detection system. Standard curve

Green supermix will be available soon and it will be compatible

Green supermix provides extreme

Green supermix. Efcient discrimination and reliable

Green supermix demonstrates superior

Green supermix showed quality amplication

supermixes ideal choices for challenging

Green supermix. Efcient discrimination and

Green supermix provides reliable gene

real-time PCR detection system. iTaq

Green supermix produced greater than 90% efciency

Green supermix allows robust

Green supermix generates precise, quantitative results.

real-time PCR detection system. Standard curve

Green I stability and performance in a

Green supermix and the iScript

cDNA synthesis kit show

DNA polymerase for quick

real-time PCR detection system. Standard curve r = 0.999,

real-time PCR detection system and genotypes were automatically

Chromatin Analysis Kit

Green supermix on the

High-Fidelity DNA Polymerase

Green/ EvaGreen Supermixes Probes Supermixes One-Step Kits for RT-

Green supermix will be available soon.

plates are designed for

Standard Hard-Shell 480

Bio-Rad DNA Engine

Semi-Skirted Hard-Shell Skirted Multiplate

Unskirted Multiplate Unskirted iQ

(high-prole plates only), S1000

(low-profile white plates recommended)

, DNA Engine Opticon

; Eppendorf Mastercycler series;

; Applied Biosystems 0.2 ml tube cyclers, real-time

; Applied Biosystems 0.1 ml

, iQ5, iCycler iQ, MyiQ, MyiQ2,

real-time PCR detection systems. The semi-skirted

, MyiQ, iCycler iQ, iQ5, MyiQ2, Chromo4

; Applied Biosystems 0.2 ml tube cyclers and

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