Research Title

Anti-angiogenic and Antibacterial Properties of Orange Mud Crab ( Scylla orivacea) Hemolymph

RESEARCH COMPETITION

Name of School
General Santos Hope Christian School

C-2
Life Science Category Do not fill this up.

FORM

School Address
Block 8, Dadiangas Heights, General Santos City

Region

XII

Contact Number

(083)552-6468

Subcategory (Check all that applies in the category) 1.) Agricultural Sciences 2.) Botany 3.) Environmental Sciences 4.) Health Sciences Name of Student Participants (Lastname, Firstname, MI) 1.) Billena, Salve Rachelle Y. 2.) 3.) Name of Adviser/Coach Nickname
Salve

5.) Microbiology 6.) Zoology Others:

Cellphone Number (9xx xxx xxxx)

E-mail Address

Note: Fill out this form completely. Do not leave any blank spaces. Pass this form on or before November 23, 2013

University of the Philippines

Academic League of Chemical Engineering Students

Research Title

Anti-angiogenic and Antibacterial Properties of Orange Mud Crab ( Scylla orivacea) Hemolymph
Subcategory (Check all that applies in the category) 1.) Agricultural Sciences 2.) Botany 3.) Environmental Sciences 4.) Health Sciences 5.) Microbiology 6.) Zoology Others:

RESEARCH COMPETITION

C-2
Life Science Category Do not fill this up.

FORM

Abstract

(Should not exceeed 250 words)

This study uses Orange Mud Crab because crab is a common food source in the Philippines influence by the seas and oceans surrounding it. The researcher wondered whether the crabs blood could help by being an anti-angiogenic and/or antibacterial agent. . In conducting the study, materials were used from the natural resources in the seas and from the laboratory. The researcher was curious is the hemolymph of Orange Mud Crab can be used as an anti-angiogenic and antibacterial. Before conducting experimentation, the researcher sterilized materials in the autoclave to prevent contamination. Then, the researcher tested five treatments, specifically, water (negative control), 100% hemolymph, 50% hemolymph, 25% hemolymph, and Mercapto purine (Positive control for Anti-angiogenesis) and Amoxicillin (Positive control for Antibacterial). For Anti-angiogenesis, the treatment was replicated three times on three day old duck eggs, and the results were recorded after 72 hours. For the antibacterial, the treatment was also replicated three times but on Staphylococcus aureus. The results were then recorded after 24 hours. Based on the results of the anti-angiogenesis and antibacterial experimentation, the 100% hemolymph has almost the same effect as the positive control. This means that the hemolymph of Orange Mud crab can be used as an anti-angiogenic and antibacterial. It is recommended that the future researchers could conduct an antibacterial against bacteria aside from Staphylococcus aureus. The researchers could also research what components in the crab;s hemolymph allow for it to be used as an anti-angiogenic and antibacterial.

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University of the Philippines

Academic League of Chemical Engineering Students