Code No: R050212305 Set No.

1
II B.Tech I Semester Supplementary Examinations, February 2007
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Discuss the discoveries of Redi, Needham, Spallanzani, and Ferdinand in disproving

the theory of spontaneous generation. [16]

2. Briefly describe the endospore formation and germination in Bacteria. [16]

3. Describe the viruses of eucaryotic organisms. [16]

4. How does a bacteriophage introduce its nucleic acid into host cell. [16]

5. Briefly describe in general terms the morphology and nucleic acids of the major
phage types. [16]

6. Write short notes on:

(a) Growth factors.

(b) Aerobes.
(c) Anaerobes.
(d) Nutrients. [4+4+4+4]

7. Write short notes on:

(a) Spore preparation.

(b) Lyophilisation.
(c) Cryopreservation.
(d) Stock cultures. [4+4+4+4]

8. What are the chemotherapeutic agents? Discuss the effectiveness of chemothera-

peutic agents. [16]

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Code No: R050212305 Set No. 2
II B.Tech I Semester Supplementary Examinations, February 2007
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Describe the contributions of the following people in the development of microbi-

ology a) John Tyndall b) Alexander Fleming c) John Needham d) Francisco Redi.
[4+4+4+4]

2. How PCR technique is useful in classifying bacteria. [16]

3. Mention the difference between animal and plant viruses. [16]

4. Describe the replication and transcription in RNA viruses with example. [16]

5. What is a virus? Discuss various types of viral infections. [16]

6. Write short notes on:

(a) Growth factors.

(b) Aerobes.
(c) Anaerobes.
(d) Nutrients. [4+4+4+4]

7. Define water activity and briefly discuss how it can be determined. Why is it
diffcult for microorganisms to grow at low water activity (aw) values. [16]

8. Describe surfactants, alkylating agents and heavy metals in terms of their chemical
nature as disinfectants. [16]

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Code No: R050212305 Set No. 3
II B.Tech I Semester Supplementary Examinations, February 2007
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. What are the different serological methods of identification of microorganisms?[16]

2. Write notes on:

(a) FAME
(b) Ribotyping
(c) Bacterial Speciation. [6+4+6]

3. Describe the structural properties of virus. [16]

4. Discuss general steps in viral replication. [16]

5. What is a virus? Discuss various types of viral infections. [16]

6. What are nutrients? Describe major nutritional types of microorganisms. [16]

7. Explain the influence of environmental factors on growth of microorganisms. [16]

8. Explain various methods to control microorganisms growth. [16]

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Code No: R050212305 Set No. 4
II B.Tech I Semester Supplementary Examinations, February 2007
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Describe the contributions of the following people in the development of microbi-

ology a) John Tyndall b) Alexander Fleming c) John Needham d) Francisco Redi.
[4+4+4+4]

2. Describe the eucaryotic diversity with suitable examples. [16]

3. What is ‘hepatitis’ ? Explain about hepatitis viruses. [16]

4. Explain the entry of viruses into host cells. [16]

5. How is a one step growth experiment carried out ? Summarize what occurs in each
phase of the resulting growth curve. Define latent period, eclipse period, rise period
and burst size. [16]

6. What are nutrients? Describe major nutritional types of microorganisms. [16]

7. Write the effect of the following environmental factors on the growth of microor-
ganisms.

(a) Solutes.
(b) PH
(c) Oxygen
(d) Osmotic pressure. [4+4+4+4]

8. Explain the use of physical methods in control of microorganisms growth. [16]

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