University of Miyazaki is a National university in Miyazaki, Japan.

University of Miyazaki have some faculty is faculty of Education, faculty of Agriculture and faculty of Engineering. And Prof. Ryo Akashi from Faculty of Agriculture, Department of Biological Production and Environmental Science. Plant Biotechnology Tree is basic of Science. It is estabilish of gene transformation and the final is useful of gene. Sustainable energy like sugarcane. All plant has selulose can product bioethanol with low consentration. For genetic transformation can use agrobacterium or particle bombardment. Plant Biotechnology tree is tell about: 1. Breeding Breeding is reproduction, that is, producing of offspring, usually animals or plants. 2. Micropropagation Micropropagation is the practice of rapidly multiplying stock plant material to produce a large number of progeny plants, using modern plant tissue culture methods.

Micropropagation is used to multiply novel plants, such as those that have been genetically modified or bred through conventional plant breedingmethods. It is also used to provide a sufficient number of plantlets for planting from a stock plant which does not produce seeds, or does not respond well to vegetative reproduction. 3. Conversation of genetic resource Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics through the rapid publication of technical papers and reviews on methodological innovations and improvements, computer programs and genomic resources. 4. Sustainable energy Sustainable energy is the sustainable provision of energy that meets the needs of the present without compromising the ability of future generations to meet their needs. 5. Productions of useful livestock Livestock production is likely to be increasingly affected by carbon constraints and environmental and animal welfare legislation. 6. Bioremediation

Bioremediation is a waste management technique that involves the use of organisms to remove or neutralize pollutants from a contaminated site. 7. Productions of useful metabolities Example is plant tissue culture, callus and pollen. Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. 8. Genetic transformation Transformation in genetic alteration of a cell resulting from the direct uptake,

incorporation and expression of exogenousgenetic material (exogenous DNA) from its surroundings and taken up through the cell membranes.

Particle bombardment is a technique which can be used to introduce foreign DNA to a cell culture. Its use gene gun to deliver particle into a cell culture. This technique ussually usein laboratory for research. Its use specialized equipment. Particle bombardment, particle shoot to cell with helium gas. Particle bombardment use for callus and usually use for monocotil plant. Agrobacterium monokotil and dicotil, 5 gain for fumctions, 4 normal and 1 mutan. Monocotil gen (rh gen) are 6. Normal gen 5 andmutan gen 1. Dicotil with agrobacterium. Example is protoplast culture system. Functional analysis of genes following fox hunting system. Advantage of agrobacterium is useless of other equipment, adaptable for various tissue. But limitation plant species also difficult to monocot. Particle bombardment is adaptable with plant species. But high cost of the equipment. Particle bombardment need high pressure to shoot because use helium gas. Also can use direct transfer of DNA. The advantage is adaptable plant species but necessary to estabilish protoplast culture system. To analysis loss of function gene in plant, use microarray. It is do by computer. Its use for analysis loss of function and gain of function. FOX (Fulllenght Over Xpressor gen) hunting system for gain of function. Its has advantage possible to search function and time saving.