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Simply better membrane technology, simply better Stericup lters.

Dont take risks with your media. If your sterile ltration membranes are clogging and/or binding serum proteins in your media, your cells risk: Cellular damage Exposure to toxins Suboptimal cell growth Trust Stericup lters with NEW improved Millipore Express PLUS membrane. Our most popular Stericup membrane, Millipore Express PLUS polyethersulfone (PES), now delivers even faster ltration rates while reducing overall protein and reagent loss.

Learn more at: www.emdmillipore.com/sterileltration

Membrane design makes a difference.


Membrane Millipore Express PLUS PES (polyethersulfone) Asymmetric Pore Structure Membrane Top Membrane Bottom Main Features High ow rate Low protein binding Low volume retention

Protein Binding by Membrane Type


Durapore (PVDF) Membrane Millipore Express PLUS (PES) Membrane Cellulose acetate Nylon 0 100 200 300 400

Low Protein Binding: Minimizes Protein Loss


Total Protein Loss (g/cm2) 200 150 100 50 0

T tor eti 2 mp ) Co cm (69 CT tor eti 2 mp ) Co 4 cm M (6 W tor eti 2 mp ) Co cm (64 CN tor eti 2 mp ) Co 5 cm F R (5 NL tor eti 2 mp ) Co cm (64 ilter F p cu 2 ) eri m St 42 c (

IgG Protein Bound to Membrane (g/cm2)

13 mm disks from were cut out from different ltration devices (n=3). Protein binding was quantied by incubating discs in 1 mL tracer (1 mg/mL goat IgG in PBS containing 125I-goat IgG at 0.1 Ci/mL). After 2 h of shaking at RT, the tracer solution was aspirated. Disks were washed 3X in PBS then assayed for bound radioactivity.

EMD Millipore is a division of Merck KGaA, Darmstadt, Germany

Simply better Stericup lters.


Faster ltration.
Flow Rate (mL/sec) Flow Rate (mL/sec) 30 20 10 0 30 20 10 0 Co Ste C C Co Co Co S C C Co Co m ri (4 ter(i6 omp (6 omp m m m (55 omp (55 o(m (6p 6p mpe (64 m 4 t pet(64 c (6p4etc pet(64 c (4p 2etci cup 2 c c9 eti 9 c eti up cm 2etito cm 42ectm c i ito2 tito cm 2ec im i i m m m m t m t t t t t t m m 2 2 F)2il or T )2 or T ) r C ) )r C r C ) or)2 C or N )2 or)2 N or N )2 or) NL Filt ) N N T T F ter F LR RF er F

Lower retention volume.


10 Volume (mL) Volume (mL) 8 6 4 2 0 10

Membrane Membrane Filtration Filtration Rates Rates (DMEM/10% (DMEM/10% FBS) FBS)

Media Media Retention Retention Volume Volume (DMEM/10% (DMEM/10% FBS) FBS)

8 6 4 2 0 S S C C C C C C C C (4 teric (4 teric(64 omp (64 omp(55 omp (55 omp (69 omp (69 omp (64 omp (64 omp 2 c up 2 c up c eti c eti c eti c eti c eti c eti c eti c eti m m m m m m m t t t t t t t m 2 F m 2 F )2 or )2 or )2 or )2 or )2 or )2 or )2 or m )2 tor ) ilt ) ilt NL NL T T C CT CN CN er er T RF RF

500 mL of DMEM+10%FBS was ltered to completion through various 0.22 m hydrophilic PES membrane ltration devices. Filtration rates and retention volumes were calculated (n=3).

Stericup and Steritop Filter Units


Description Membrane Pore Size (m) Funnel Capacity (mL) Receiver Bottle (mL) Qty/Pk Catalogue No.

Stericup-GP Filter Unit

Millipore Express PLUS (PES)

0.22 0.22 0.22 0.22 0.22 0.22 0.22 0.22 0.22 0.22 0.22

150 250 500 1000 150 150 250 250 500 500 1000

150 250 500 1000 150 150 250 250 500 500 1000

12 12 12 12 12 12 12 12 12 12 12

SCGPU01RE SCGPU02RE SCGPU05RE SCGPU11RE SCGPS01RE SCGPT01RE SCGPS02RE SCGPT02RE SCGPS05RE SCGPT05RE SCGPT10RE

Steritop-GP Filter Unit

Millipore Express PLUS (PES)

Steriip Filter Units


Description Membrane Volume (mL) Pore Size (m) Qty/Pk Catalogue No.

Steriip-GP Filter Unit Steriip-GV Filter Unit Steriip-HV Filter Unit

Millipore Express PLUS (PES) Durapore (PVDF) Durapore (PVDF)

50 50 50

0.22 0.22 0.45

25 25 25

SCGP00525 SE1M179M6 SE1M003M00

Sterile 33 mm Millex Syringe Filters


Description Membrane Pore Size (m) Qty/Pk Catalogue No.

Millex-GP Filter Unit

Millipore Express PLUS (PES)

Millex-GV Filter Unit

Durapore (PVDF)

0.22 0.22 0.22 0.45 0.22 0.45

1000 250 50 50 50 50

SLGP033RK SLGP033RB SLGP033RS SLHP033RB SLGV033RS SLHV033RS

To Place an Order or Receive Technical Assistance


In the U.S. and Canada, call toll-free 1-800-645-5476 For other countries across Europe and the world, please visit: www.emdmillipore.com/ofces For Technical Service, please visit: www.emdmillipore.com/techservice

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www.emdmillipore.com/offices
EMD Millipore and the M mark are trademarks and Stericup, Millipore Express, Durapore, Millex, Steritop, and Steriip are registered trademarks of of Merck KGaA, Darmstadt, Germany. Lit No. PR2244EN00 BS-GEN-13-08974 Printed in the USA. 08/2013 2013 EMD Millipore Corporation, Billerica, MA USA. All rights reserved.

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