Code: 9A23304

1
MICROBIOLOGY
(Biotechnology)

B.Tech II Year I Semester (R09) Regular & Supplementary Examinations December/January 2013/14

Time: 3 hours Answer any FIVE questions All questions carry equal marks
***** 1 2 3 4 5

Max Marks: 70

Describe the scope of microbiology in genetic engineering & biotechnology. Explain the various classical characteristics used in classification of microorganism. Describe the composition and properties of different types of media. Explain the effect of temperature, pH and osmotic pressure on microbial growth. What are the disease causing pathogens? Explain about pathogenicity, laboratory diagnosis and virulence factor of any one pathogen. Describe with suitable examples viruses of arthropods. Write an essay on transmission of viruses Discuss the different ways in which viruses may be cultivated.

6 7 8

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Code: 9A23304

2
MICROBIOLOGY
(Biotechnology)

B.Tech II Year I Semester (R09) Regular & Supplementary Examinations December/January 2013/14

Time: 3 hours Answer any FIVE questions All questions carry equal marks
***** 1

Max Marks: 70

Explain the experiments carried out by various scientists led to formation of Theory of spontaneous generation and how it was finally disproved. Describe the structure and functions of the bacterial cell wall with special reference to gram negative bacteria. Explain about the nutritional classes of bacteria in detail with suitable example. Explain about isolation, identification and preservation of bacteria. Discuss pathogenesis, epidemiology, and laboratory diagnosis of influenza virus infection. Describe in detail the various steps involved in replication of lysogenic / temperate phage with illustration. Explain the classification of animal and plant viruses. Describe applications of bacterial viruses in biotech industry. Briefly describe the replication process in ds DNA bacteriophage.

3 4 5 6

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Code: 9A23304

3
MICROBIOLOGY
(Biotechnology)

B.Tech II Year I Semester (R09) Regular & Supplementary Examinations December/January 2013/14

Time: 3 hours Answer any FIVE questions All questions carry equal marks
***** 1 2 Describe the important contributions made by Louis Pasteur.

Max Marks: 70

What are the three domains of Carl Woese classification? Compare the characteristics of these domains. Write an account on different anaerobic culturing techniques. Explain the methods for maintenance and preservation of microbial cultures. Discuss etiology, pathogenesis and laboratory diagnosis of influenza. Explain how a typical retrovirus replicates. Define eclipse period. Compare the lytic cycle with Iysogenic cycle. (a) (b) Distinguish viroids and prions. How do you determine the purity of viruses? *****

3 4 5 6 7 8

Code: 9A23304

4
MICROBIOLOGY
(Biotechnology)

B.Tech II Year I Semester (R09) Regular & Supplementary Examinations December/January 2013/14

Time: 3 hours Answer any FIVE questions All questions carry equal marks
***** 1 2 3 (a) (b) (c) (d) 4 5 6 7 8

Max Marks: 70

Write about the contribution of Robert Koch with respect to germ theory of diseases. Compare and contrast Carl Woeses and Whittakers classification of living organisms. Write notes on: Enriched media. Spread plate technique. Streak plate technique. Pour plate method. Explain various biochemical tests for microorganisms and colony characterization. Discuss etiology, pathogenesis and laboratory diagnosis of tuberculosis. Define prions. Classify prion diseases and discuss their pathogenesis and diagnosis. Describe the chemical structure of viruses. Explain the assays for bacterial and animal viruses.

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