Version 2.

0, valid from 2012-01-01

EUCAST recommended strains for internal quality control

Version 2.0, valid from 2012-01-01

Routine quality control strains

Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus Enterococcus faecalis Streptococcus pneumoniae Haemophilus influenzae ATCC 25922 ATCC 27853 ATCC 29213 ATCC 29212 ATCC 49619 NCTC 84681

Version number
2.0, 2012-01-01

Strain
ATCC 25922 ATCC 29213 ATCC 29212 ATCC 49619 NCTC 8468

Changes
Cells containing a change or an addition are marked yellow. QC ranges added: Cefalexin (MIC) and cefoxitin. QC ranges added: Ampicillin (zone), benzylpenicillin (MIC) and telavancin (MIC). QC ranges removed3: Tetracycline. QC ranges updated2: Benzylpenicillin (zone). QC ranges added: Amoxicillin (MIC) and nitrofurantoin (MIC and zone). QC ranges updated2: Amoxicillin-clavulanic acid (zone) and ceftibuten (zone). QC ranges added: Benzylpenicillin (zone). QC ranges removed3: Amoxicillin, ampicillin-sulbactam and phenoxymethylpenicillin.

NCTC 8468 is susceptible to beta-lactam antibiotics and easier to read on MH-F than the commonly recommended ATCC 49247 which is a BLNAR with variable inhibition zones for beta-lactam antibiotics. 2 Updated by EUCAST and/or CLSI. 3 Antibiotics not in EUCAST Clinical Breakpoint Table or no data for MIC ranges.

Version 2.0, valid from 2012-01-01

Escherichia coli ATCC 25922

(NCTC 12241, CIP 76.24, DSM 1103, CCUG 17620, CECT 434) Mueller-Hinton agar, McFarland 0.5, air, 351C, 182h. Read zone edges as the point showing no growth viewed from the back of the plate against a black background illuminated with reflected light. MIC (mg/L) Target1 1-2 8 4/2 4 4/2 0.12 8 0.03-0.06 0.5 0.06 4 0.5 0.12-0.25 0.25 0.06 4 4 0.008 1 0.03 0.008 1 0.5 0.12 0.015-0.03 0.06-0.12 0.015-0.03 0.015-0.03 2 8 0.06 0.03-0.06 2 2/4 8 8/2 0.12 0.5 1 0.5/9.52 Range2 0.5-4 4-16 2/1-8/4 2-8 2/1-8/4 0.06-0.25 4-16 0.015-0.12 0.25-1 0.03-0.12 2-8 0.25-1 0.06-0.5 0.12-0.5 0.03-0.12 2-8 2-8 0.004-0.015 0.5-23 0.015-0.06 0.004-0.015 0.5-23 0.25-1 0.06-0.25 0.008-0.06 0.03-0.25 0.008-0.06 0.008-0.06 1-4 0.5-1 4-16 0.03-0.12 0.015-0.12 1-4 1/4-4/4 4-16 4/2-16/2 0.03-0.25 0.25-1 0.5-2 Disk content (g) 30 20-10 10 10-10 30 30 30 30 5 5 30 10 10 30 30 30 30 5 10 10 10 10 5 10 10 5 30 10 100 10 5 30 30-6 75 75-10 15 10 5 1.25-23.75 Inhibition zone size (mm) 1 Target Range3 23 19-26 21 18-244 19 16-224 22 19-244 32 28-36 17 14-20 17 14-20 34 31-37 25 23-27 28 25-31 26 23-29 26 23-28 26 23-29 31 27-35 32 29-35 23 20-26 24 21-27 35 30-40 31 27-35 33 29-36 23 19-26 29 26-32 33 29-37 27 24-30 31 28-34 32 28-35 25 22-28 21 18-24 20 17-23 32 28-35 31 29-33 24 21-27 24 21-27 27 24-30 27 24-30 24 20-27 22 18-26 25 21-28 26 23-29

Antimicrobial agent Amikacin Amoxicillin Amoxicillin-clavulanic acid Ampicillin Ampicillin-sulbactam Aztreonam Cefadroxil Cefalexin Cefepime Cefixime Cefotaxime Cefoxitin Cefpodoxime Ceftazidime Ceftibuten Ceftriaxone Cefuroxime Chloramphenicol Ciprofloxacin Colistin Doripenem Ertapenem Fosfomycin5 Gentamicin Imipenem Levofloxacin Mecillinam Meropenem Moxifloxacin Nalidixic acid Netilmicin Nitrofurantoin Norfloxacin Ofloxacin Piperacillin Piperacillin-tazobactam Ticarcillin Ticarcillin-clavulanic acid Tigecycline5 Tobramycin Trimethoprim Trimethoprim-sulfamethoxazole
1 2

Calculated by EUCAST International Standard ISO 20776-1: 2006. 3 Clinical and Laboratory Standards Institute, M100-S21: 31:1, 2011. (Data in bold/italics from repeated testing by EUCAST. Inhibition zone diameters were measured on at least 20 different occasions on more than five batches of Mueller-Hinton agar from several manufacturers.) 4 Ignore growth that may appear as a thin inner zone on some batches of Mueller-Hinton agars. 5 For MIC test conditions, see Clinical and Laboratory Standards Institute, M100-S21: 31:1, 2011. IP = In Preparation

Version 2.0, valid from 2012-01-01

Pseudomonas aeruginosa ATCC 27853

(NCTC 12903, CIP 76.110, DSM 1117, CCUG 17619, CECT 108) Mueller-Hinton agar, McFarland 0.5, air, 351C, 182h. Read zone edges as the point showing no growth viewed from the back of the plate against a black background illuminated with reflected light. MIC (mg/L) Target1 2 4 2-4 2 0.5 1-2 0.25 4 1 2 1-2 0.5 2 2-4 2-4/4 16 16/2 0.5 Range2 1-4 2-8 1-8 1-4 0.25-1 0.5-43 0.12-0.5 2-83 0.5-2 1-4 0.5-4 0.25-1 0.5-8 1-8 1/4-8/4 8-32 8/2-32/2 0.25-1 Disk content (g) 30 30 30 10 5 10 10 10 5 10 10 30-6 75-10 10 Inhibition zone size (mm) 1 Target Range3 22 18-26 26 23-29 27 24-30 24 21-27 29 25-33 32 28-35 19 16-21 24 20-28 23 19-26 30 27-33 18 15-21 26 23-29 24 20-28 22 19-25

Antimicrobial agent Amikacin Aztreonam Cefepime Ceftazidime Ciprofloxacin Colistin Doripenem Fosfomycin4 Gentamicin Imipenem Levofloxacin Meropenem Netilmicin Piperacillin Piperacillin-tazobactam Ticarcillin Ticarcillin-clavulanic acid Tobramycin
1 2

Calculated by EUCAST International Standard ISO 20776-1: 2006. 3 Clinical and Laboratory Standards Institute, M100-S21: 31:1, 2011. (Data in bold/italics from repeated testing by EUCAST. Inhibition zone diameters were measured on at least 20 different occasions on more than five batches of Mueller-Hinton agar from several manufacturers.) 4 For MIC test conditions, see Clinical and Laboratory Standards Institute, M100-S21: 31:1, 2011.

Version 2.0, valid from 2012-01-01

Staphylococcus aureus ATCC 29213*

(NCTC 12973, CIP 103429, DSM 2569, CCUG 15915, CECT 794) * -lactamase-producing strain (weak) Mueller-Hinton agar, McFarland 0.5, air, 351C, 182h. Read zone edges as the point showing no growth viewed from the back of the plate against a black background illuminated with reflected light. MIC (mg/L) Target1 2 1 0.5-1 2 4-8 0.25 0.25 0.12 0.25-0.5 0.25 0.5 1-2 0.12 0.25-0.5 0.12-0.25 2 0.12-0.25 0.03-0.06 0.12 0.252 16 1 0.25-0.5 0.5 0.008 0.5 0.25-0.5 0.25-0.5 0.06-0.12 0.25-0.5 2 0.5/9.52 1 Range2 1-4 0.5-2 0.25-2 1-4 2-16 0.12-0.5 0.12-0.5 0.06-0.25 0.12-1 0.12-0.5 0.25-1 0.5-43 0.06-0.25 0.12-1 0.06-0.5 1-4 0.06-0.5 0.015-0.12 0.06-0.25 8-32 0.5-2 0.12-1 0.25-1 0.004-0.015 0.25-1 0.12-1 0.12-1 0.03-0.25 0.12-1 1-4 0.5-2 Disk content (g) 30 2 1 unit 30 30 5 2 15 10 10 5 10 30 5 200 10 100 10 5 15 5 30 15 10 5 1.25-23.75 Inhibition zone size (mm) Target1 Range3 21 18-24 18 15-21 15 12-18 27 24-30 24 20-28 24 21-27 26 23-29 26 23-29 29 26-32 22 19-25 26 23-29 24 21-27 26 23-29 28 25-31 34 31-37 23 20-26 20 17-23 21 18-24 24 21-27 24 21-27 33 30-36 27 23-31 22 19-25 23 20-26 25 22-28 29 26-32 -

Antimicrobial agent Amikacin Ampicillin Azithromycin Benzylpenicillin Cefoxitin Chloramphenicol Ciprofloxacin Clarithromycin Clindamycin Daptomycin4 Doxycycline Erythromycin Fosfomycin4 Fusidic acid Gentamicin Levofloxacin Linezolid Minocycline Moxifloxacin Mupirocin Netilmicin Nitrofurantoin Norfloxacin Ofloxacin Quinupristin-dalfopristin Rifampicin Teicoplanin Telavancin Tetracycline Tigecycline4 Tobramycin Trimethoprim Trimethoprim-sulfamethoxazole Vancomycin
1 2

Calculated by EUCAST International Standard ISO 20776-1: 2006. 3 Data in bold/italics from repeated testing by EUCAST. Inhibition zone diameters were measured on at least 20 different occasions on more than five batches of Mueller-Hinton agar from several manufacturers. 4 For MIC test conditions, see Clinical and Laboratory Standards Institute, M100-S21: 31:1, 2011. IP = In Preparation

Version 2.0, valid from 2012-01-01

Enterococcus faecalis ATCC 29212

(NCTC 12697, CIP 103214, DSM 2570, CCUG 9997, CECT 795) Mueller-Hinton agar, McFarland 0.5, air, 351C, 182h. Read zone edges as the point showing no growth viewed from the back of the plate against a black background illuminated with reflected light. MIC (mg/L) Target1 1 8 1 2 8 4 0.12 0.06 12 0.5/9.52 2 Range2 0.5-2 4-16 0.5-2 1-4 4-16 2-8 0.06-0.25 0.03-0.12 1-4 Disk content (g) 2 304 10 10 100 15 30 15 5 1.25-23.75 5 Inhibition zone size (mm) 1 Target Range3 18 15-21 15 12-18 27 24-30 22 19-25 21 18-24 14 11-17 18 15-21 23 20-26 28 24-32 30 26-34 13 10-16

Antimicrobial agent Ampicillin Gentamicin Imipenem Linezolid Nitrofurantoin Quinupristin-dalfopristin Teicoplanin Tigecycline5 Trimethoprim Trimethoprim-sulfamethoxazole Vancomycin
1 2

Calculated by EUCAST International Standard ISO 20776-1: 2006. 3 Data in bold/italics from repeated testing by EUCAST. Inhibition zone diameters were measured on at least 20 different occasions on more than five batches of Mueller-Hinton agar from several manufacturers. 4 Screening disk for high-level aminoglycoside-resistance in enterococci. 5 For MIC test conditions, see Clinical and Laboratory Standards Institute, M100-S21: 31:1, 2011.

Version 2.0, valid from 2012-01-01

Streptococcus pneumoniae ATCC 49619*/**

(NCTC 12977, CIP 104340, DSM 11967, CCUG 33638) * Penicillin-intermediate strain ** Zone edges for S. pneumoniae on MH-F may be accompanied by -haemolysis extending approximately 1 mm into the zone. Read growth, not haemolysis. With some batches of agar and some antibiotics the colonies at the zone edge lyse and the apparent width of haemolysis is considerably greater. The zone edge should be read as the edge of the lysed colonies indicated by a haze just inside the -haemolysis. Mueller-Hinton agar + 5% horse blood and 20 mg/L -NAD, McFarland 0.5, 5% CO 2, 351C, 182h. Read zone edges as the point showing no growth viewed from the front of the plate with the lid removed and with reflected light. MIC (mg/L) Target1 0.06 0.12 0.12 0.5 2 0.06-0.12 0.06 0.06 0.06 0.5 4 0.06 0.06 0.12-0.25 0.06 0.03-0.06 0.06-0.12 0.06 0.06 1 0.5-1 0.12 0.12 8 4 2 0.03 0.008-0.015 0.25 0.03-0.06 0.25/4.75-0.5/9.5 0.25 Range2 0.03-0.12 0.06-0.25 0.06-0.25 0.25-1 1-4 0.03-0.25 0.03-0.12 0.03-0.12 0.03-0.12 0.25-1 2-8 0.03-0.12 0.03-0.12 0.06-0.5 0.03-0.12 0.015-0.12 0.03-0.25 0.03-0.12 0.03-0.12 0.5-2 0.25-2 0.06-0.25 0.06-0.25 4-16 2-8 1-4 0.015-0.06 0.004-0.03 0.12-0.5 0.015-0.12 0.12/2.4-1/19 0.12-0.5 Disk content (g) 2 1 unit 30 30 5 10 30 30 30 5 2 10 10 15 10 5 10 10 30 5 100 10 5 1 5 30 15 30 15 1.25-23.75 5 Inhibition zone size (mm) 1 Target Range3 28 25-31 19 16-22 28 25-31 34 31-37 31 28-34 32 29-35 35 32-38 31 28-34 27 24-30 25 22-28 25 22-28 34 31-37 31 28-34 29 26-32 38 34-42 24 21-27 26 23-29 34 30-38 28 25-31 27 24-30 28 25-31 21 18-24 21 18-24 11 8-14 29 26-32 21 18-24 30 27-33 31 28-34 27 24-30 23 20-26 20 17-23

Antimicrobial agent Amoxicillin Ampicillin Azithromycin Benzylpenicillin Cefaclor Cefepime Cefotaxime Cefpodoxime Ceftriaxone Cefuroxime Chloramphenicol Ciprofloxacin Clarithromycin Clindamycin Daptomycin4 Doripenem Doxycycline Ertapenem Erythromycin Imipenem Levofloxacin Linezolid Meropenem Minocycline Moxifloxacin Nitrofurantoin Norfloxacin Ofloxacin Oxacillin Rifampicin Teicoplanin Telithromycin Tetracycline Tigecycline5 Trimethoprim-sulfamethoxazole Vancomycin
1 2

Calculated by EUCAST International Standard ISO 20776-1: 2006. 3 Data in bold/italics from repeated testing by EUCAST. Inhibition zone diameters were measured on at least 20 different occasions on more than five batches of Mueller-Hinton agar from several manufacturers. 4 For MIC test conditions, see Clinical and Laboratory Standards Institute, M100-S21: 31:1, 2011. IP = In Preparation

Version 2.0, valid from 2012-01-01

Haemophilus influenzae NCTC 8468

(CIP 54.94, CCUG 23946) Mueller-Hinton agar + 5% horse blood and 20 mg/L -NAD, McFarland 0.5, 5% CO 2, 351C, 182h. Read zone edges as the point showing no growth viewed from the front of the plate with the lid removed and with reflected light. MIC (mg/L) Target IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP Range IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP IP Disk content (g) 2-1 2 1 unit 30 30 5 5 10 30 30 30 30 5 10 10 15 10 5 10 30 5 30 5 5 15 30 1.25-23.75 Inhibition zone size (mm) 1 Target Range1 20 17-23 22 19-25 16 13-19 27 24-30 32 29-35 30 27-33 32 29-35 31 28-34 33 30-36 37 33-41 28 25-31 34 30-38 35 31-39 29 26-32 30 27-33 15 12-18 28 25-31 35 32-38 31 28-34 30 27-33 32 29-35 30 27-33 33 30-36 23 20-26 18 15-21 31 28-34 30 26-34

Antimicrobial agent Amoxicillin-clavulanic acid Ampicillin Benzylpenicillin Cefaclor Cefepime Cefixime Cefotaxime Cefpodoxime Ceftibuten Ceftriaxone Cefuroxime Chloramphenicol Ciprofloxacin Doripenem Ertapenem Erythromycin Imipenem Levofloxacin Meropenem Minocycline Moxifloxacin Nalidixic acid Ofloxacin Rifampicin Telithromycin Tetracycline Trimethoprim-sulfamethoxazole
1 2

Calculated by EUCAST Data in bold/italics from repeated testing by EUCAST. Inhibition zone diameters were measured on at least 20 different occasions on more than five batches of Mueller-Hinton agar from several manufacturers. IP = In Preparation