Single factor experiments

1-Completely randomized design (CRD)

1- Randomization and Layout:
Experiment had 5 treatment and 3 replicate
Total number of plots = t x r = 15
Treatments = A, B, C, D, F
1A 2D 3C
4D 5F 6B
7B 8A 9F
10 F 11 C 12 A
13 C 14 B 15 D

Treatment R1 R2 R3 Treatment Treatment

Total mean
1 1 2 3 6 2
2 5 6 7 18 6
3 9 10 11 30 10
4 13 14 15 42 14
5 17 18 19 54 18
Grand Total (G) 150
Grand mean 10

Correction factor(C.F)= G2/n = 1500

Total SS = ∑ X -C.F
2

Treatment SS = ∑ T / r
2

Error SS = Total SS- Treatment SS

Total SS = [ (1)2 + (2)2+ ………………..(19)2]- 1500
Treatment SS = [ (6)2 + (18)+………(54)] - C.F
Treatment MS = Treatment SS/ (t-1)
Error MS = Error SS / t(r-1)

F= Treatment MS / Error MS
Table : Analysis Of Variance (CRD)
Source of Degree of Sum of Mean Computed Tabular F
Variations freedom Squares Square F
Treatments t-1
Experimental t(r-1)
error
Total t.r -1
2- Randomized Complete Block Design (RCB)

1-Randomization and Layout:

Experiment had 5 treatment and 3 replicate
Treatments = A, B, C, D, F

R1 R2 R3
A C D
C F A
B B C
D D F
F A B

Treatment R1 R2 R3 Treatment Treatment

Total mean
1 1 2 3 6 2
2 5 6 7 18 6
3 9 10 11 30 10
4 13 14 15 42 14
5 17 18 19 54 18
Rep. Total(R) 45 50 55
Grand Total (G) 150
Grand mean 10

Correction factor(C.F)= G2/n = 1500

Total SS = ∑ X -C.F
2

Replication SS = ∑ R / t -C.F
2

Treatment SS = ∑ T / r -C.F
2

Error SS = Total SS- Treatment SS – Replication SS

Total SS = [ (1)2 + (2)2+ ………………..(19)2]- 1500
Replication SS = [(45)2+ (50)2+(55)2]/t -1500

Treatment SS = [ (6)2 + (18)+………(54)] /r -1500

Treatment MS = Treatment SS/ (t-1)
Error MS = Error SS / (t-1)(r-1)

F= Treatment MS / Error MS
Table : Analysis Of Variance (CRD)
Source of Degree of Sum of Mean Computed Tabular F
Variations freedom Squares Square F
Replication r-1
Treatments t-1
Experimental (t-1)(r-1)
error
Total t.r -1
3- Latin Square Design
1-Randomization and Layout:
Experiment had 5 treatment and 5 replicate
Treatments = A, B, C, D, F
E C B A D
A D C B E
C B D E A
B E A D C
d A E C B
2-Analysis of variances
Experiment had 4 treatment and 4 replicate
Treatments = A, B, C, D

Table : grain yield of three Maize Hybrids and check from an experiment with
Latin square design.
Row Number Col1 Col2 Col3 Col4 Row total
1 B6 D 17 C 13 A4 40
2 C 11 A2 D 18 B9 40
3 A1 C 12 B8 D 19 40
4 D 16 B7 A3 C 14 40
Column total (C) 34 38 42 46
Grand total 160

Treatment Total Mean

A 10 2.5
B 30 7.5
C 50 12.5
D 70 17.5
2 2
Correction factor(C.F)= G /t = 1600
Total SS = ∑ X -C.F
2

Row SS = ∑ R / t -C.F
2

Column SS = ∑ C / t -C.F
2

Treatment SS = ∑ T / r -C.F
2

Error SS = Total SS- Row SS – Column SS – Treatment SS

Total SS = [ (1)2 + (2)2+ ………………..(19)2]- 1600

Row SS = [(40)2+ (40)2+(40)2]/t –1600
Column SS = [(34)2+(38)2+(42)2+(46)2]/t -1600
Treatment SS = [ (10)2 + (30)2+………(70)2] /r –1600

Row MS = Row SS/(t-1)

Column MS = Column MS / (t-1)
Treatment MS = Treatment SS/ (t-1)
Error MS = Error SS / (t-1)(t-2)
 Table : Analysis Of Variance Latin square
Source of Degree of Sum of Mean Compute Tabular F
Variations freedom Squares Square dF
Row t-1
Column t-1
Treatments t-1
Experimental error (t-1)(t-2)
Total t2 -1

Two factor experiments

1-Factorial Experiment:
Complete block design
1-Randomization and Layout:
Experiment had 2 factor 3 level of N and 2 level of P and 3 replicate
N level = N1, N2, N3
P level = P1, P2
Table combination treatments of 2 factor 3 level of N and 2 level of P
P treatment
Nitrogen Level P1 P2
N1 N1P1 N1P2
N2 N2P1 N2P2
N3 N3P1 N3P2

R1 R2 R3
N1P2 N3P1 N3P2
N2P1 N2P1 N1P1
N3P1 N1P2 N1P2
N1P1 N3P2 N3P1
N3P2 N1P1 N2P1
N2P2 N2P2 N2P2
Table grain yield of Corn fertilized with 3 Level of nitrogen and 2 level of P
Nitrogen level R1 R2 R3 Treatment
total
P1
N1 1 2 3 6
N2 5 6 7 18
N3 9 10 11 30
P2
N1 21 22 23 66
N2 25 26 27 78
N3 28 29 30 97
Rep. total 86 92 98
Grand total (G) 295

C.F = G2/rab
Total SS = ∑ X -C.F
2
Replication SS = ∑ R / ab -C.F
2

Treatment SS = ∑ T / r -C.F
2

Error SS = Total SS- Replication SS –Treatment SS

Table: Preliminary analysis of Variance for data
Source of Degree of Sum of Mean Computed Tabular F
Variations freedom Squares Square F
Replication r-1
Treatments ab-1
Experimental (r-1)(ab-1)
error
Total rab -1

Compute the three factorial components of the treatment sum of square as:
ASS = ∑ A / rb -C.F
2

BSS = ∑ B / ra -C.F
2

Table The Nitrogen x Phosphorus table of totals from

P Levels Nitrogen total (A)
Nitrogen levels P1 P2
N1
N2
N3
Phosphorus Total (B)

A x B SS = Treatments SS – ASS – BSS

A MS = A SS / a-1
B MS = B SS/b-1
A x B SS = A x B SS/(a-1)(b-1)
Error MS = Error SS / (r-1)(ab-1)
F (A) = A MS / Error MS
F (B) = B MS / Error MS
F (A x B) = A x B MS / Error MS
Source of Degree of Sum of Mean Computed Tabular F
Variations freedom Squares Square F
Replication r-1
Treatments ab-1
A (Nitrogen a-1
levels)
B (phosphor b-1
levels)
AxB (a-1)(b-1)
Experimental (r-1)(ab-
error 1)
Total rab -1
 2-Split Plot Design
1-Randomization and Layout:
Experiment had 2 factor 3 methods of irrigation system and 2 level of N and 3
replicate
Irrigation System = I1, I2, I3
N level = N1, N2

R1 R2 R3
Main plot I1 I2 I3
I3 I3 I2
I2 I1 I1

R1 R2 R3
I1 P1 I1 P2 I2 P2 I2 P1 I3 P1 I3 P2
I3 P2 I3 P1 I3 P2 I3 P1 I2 P1 I2 P2
I2 P1 I2 P2 I1 P2 I1 P1 I1 P2 I1 P1

Table grain yield of Corn irrigated with 3 irrigation system and fertilized with 2
level of N
Irrigated R1 R2 R3 Treatment
method total
N1
I1 1 2 3 6
I2 5 6 7 18
I3 9 10 11 30
N2
I1 21 22 23 66
I2 25 26 27 78
I3 28 29 30 97
Grand total (G) 285

Table : The replication X Irrigation method

Irrigated R1 R2 R3 Treatment
method total
I1 22 24 26 72
I2 30 32 34 96
I3 37 39 41 117
Rep total (R) 89 95 101
Grand total (g) 285
C.F = G2/rab
Table : The irrigation x Nitrogen level
Irrigated method N1 N2
I1 6 66
I2 18 78
I3 30 97
Nitrogen total (N) 54 241
Total SS = ∑ X -C.F
2
Replication SS = ∑R 2
/ab -C.F
A SS = ∑A 2
/rb -C.F
Error(A) SS = ∑ RA 2
/b -C.F
B SS = ∑ B /ra -C.F
2

A x B SS = ∑ AB /r -C.F –BSS – ASS

2

Error(b) SS = Total SS – (sum of all otherSS)

Source of Degree of Sum of Mean Computed Tabular F

Variations freedom Squares Square F
Replication r-1
Main plot a-1
factor (A)
Error (A) (r-1) (a-1)
Sub plot b-1
factor (B)
AxB (a-1)(b-1)
Error (b) a(r-1)(ab-1)
Total rab -1