Sie sind auf Seite 1von 8

BACTERIAL GENETICS

All the properties of the cell, including its virulence, pathogenicity and antibiotic
resistance, are determined by it genome. This is encoded by the specific sequences
of nucleotide bases in the DNA. Recall that the bacterial DNA is a single, circular ds-
DNA, which does NOT have a nuclear membrane and has NO histones. E.coli is an
exception since its DNA is an irregular coiled bundle lying freely in the cytoplasm.

In addition to the main ds-DNA, some bacterial cells may also carry one or more
extra-chromosomal elements, which appear circular, and are termed PLAMIDS.

Plasmids replicate INDEPENDENTLY of the main chromosome in the cell. They carry
supplemental genetic information coding for certain properties such as antibiotic
resistance or the ability to survive harsh environments.

A third source of genetic information in the bacterial cells is provided by


BACTERIOPHAGES. These are also termed bacterial viruses. They consists of a viral
genome enclosed by a protein coat/ They need a bacterial host in order to multiply,
and when they do so, they cause death of the bacterial cell. In some instances
though, they can undergo LYSOGENY-they unergo controlled, long term replication
within the bacterial cell without causing the bacterial cell’s lysis. In this way, it may
become a temporary part of the cell, and change the bacterial cell’s properties.

Table 1. Comparison between the 3 types of genetic information sources in the


bacterial cell

Genetic element TYPE Configuration Size in kilobases

Bacterial chromosome DNA ds circular 2.0 to 4.0 x10

Plasmids DNA ds circular 9 to 60

Bacteriophages RNA/DNA ss/ds 3 to 40

Linear/circular

3 Principal mechanisms of genetic material transfer into a HOST organism:

1. TRANSFORMATION

2. TRANSDUCTION

3. CONJUGATION
TRANSFORMATION

Transformation is the uptake and integration of naked DNA from the environment
into the host cell. Once inside the cell, there must be homologous recombination
with the chromosome of the host.

Most species of bacteria are unable to take up exogenous DNA from their
environment; in fact, they produce nucleases to recognize and breakdown these
foreign DNA. Haemophilus and certain Bacillus however, can take up DNA through
transformation. They are able to do this only in LOG PHASE or in the period of
exponential growth. The Bacillus species will take it up in their SPORE phase.

Transformation can be induced artificially in the lab. Incubation of E.coli at 4C, in the
presence of calcium ions, then a short exposure to 42C will allow structural
alterations in the cell wall, allowing passage of the DNA into the E.coli cell.

In general, ANY gene may be transferred by transformation, however, this fragment


is usually small and will contain a limited number of genes.

Once inside the bacterial cell, the DNa has to be incorporated into the existing
chromosome through RECOMBINATION in order to survive. The pieces of DNA can
only recombine with the chromosome if there is a high degree of nucleic acid
similarity, also known as HOMOLOGY.

Recall that the bases in the DNA are paired in such a way: adenine always pairs with
thymine, and guanine always pairs with cytosine.

An example of this was a study conducted by Dr Frederick Griffith in the 1920’s


using S.pneumoniae. After injecting heat-killed S colonies with R colonies into mice,
he was able to isolate live S colonies. He concluded that the heat released the gene
encoding for the capsule, and was taken up into the R colonies, thus transforming
them into S colonies.

TRANSDUCTION

This is the PHAGE-MEDIATED transfer of bacterial DNA.

PHAGES are bacterial viruses with a DNA molecule enclosed in a protein shell. The
structure of the protein shell imposes a limitation on the amount of DNA that can be
enclosed by the virus, usually about 50 kilobases.

Most phages carry ds-DNA coiled up inside a protein coat. There may be species of
phages which have a ss-DNA or ss-RNA as well.
There are two kinds:

1. Generalized transduction-

When phages enter a bacterial cell and replicate, each phage head usually
has a copy of the replicated phage genome. Sometimes, an empty phage
head is produced. Bacterial DNA may be mistakenly packaged into that
empty phage head, then transferred to another bacterial cell. Thus, the DNA
that enters the second bacterial cell is a short segment of the chromosome of
the first bacterial cell. As with transformation, recombination must occur for
transduction to be successful.

It is termed generalized since the phage will pick up any portion of the
bacterial chromosome at random (is is not specific for a particular
chromosomal segment).

Phages usually attack a limited range of bacteria, so there is a close relation


between these specias, so the genes transferred are within a certain species
only.

Phages can also pick up and transfer plasmid DNA. For example, the
penicillinase gene of Staphlococci is located in a plasmid, and the phages can
pick up that plasmid and through transduction, pass it on to another cell, thus
transferring the penicillanase gene to other staphylococci species.

2. Specialized transduction-

Bacteriophages that lyse the host cell are known as VIRULENT PHAGES, and
cause a LYTIC cycle of infection. In contrast, phages that can infect a
bacterial cell without causing its death are called TEMPERATE PHAGES.

The bacterial cell which survives after phage infection is called a LYSOGEN,
and the integrated temperate phage inside of it is termed a PROPHAGE.

The phage DNA is then inserted in to the bacterial cell DNA,and is also
replicated as part of the host cell chromosome.

Can more that 1 phage infect a bacterial cell?

No. Once the phage is integrated into the bacterial cell DNA, it imparts
immunity against superinfection by other phages.
The lysogen phase is stable but not permanent. If the prophage is excised
during the process of replication, the bacterial cell may eventually be lysed.
This can be replicated in the lab by exposure of bacteria to UV light. (e.g.
hospital sterilization of rooms with UV).

In contrast to generalized transduction, temperate phages normally have a


specific insertion site on the chromosome and can pick up only a short length
of DNA containing a few genes on either side of this site. Thus, it is termed
SPECIALIZED OR RESTRICTIVE TRANSDUCTION.

Defective phages can occur. As it attaches to the bacterial DNA chromosome


and picks up DNA adjacent to the phage integration site, the amount of DNA
may be too much, thus the phage will lack a few phage genes. When that
defective phage is transduced to a second bacterial cell, the phage can still
integrates into its phage specific site, but it cannot replicate normally nor can
it lyse the bacterial cell. The final product is the integration of the 1 st bacterial
cell’s DNA into the 2nd bacteria’s DNA.

How does this influence the virulence of the bacteria for humans?

Since the prophage carries genes, its presence in a bacterial cell may cause the
expression of certain proteins. This is called LYSOGENIC CONVERSION. For example,
the diphtheria toxin of Corynebacterium diptheriae will only be produced if the Beta
Phage infects the bacteria.

Another common use for the phages are the LAMBDA PHAGES of E.coli. They have a
genome of 50 kilobases, but one the nonessential genes are removed, they are a
reduced to 30 kilobases, making room for the insertion ofr foreign DNA. The phage
then infects E.coli, which in turn replicates along with the phage (phage-
synthesizing factory), then the E.coli cell is lyzed and the phages infect other cells.

Why E.coli? it is the ideal host since it replicates every 20 minutes. Also, E.coli has
all the distinct phases in the bacterial growth curve, so its growth can be monitored
in the lab.

CONJUGATION
This is the most important mechanism for widespread transfer of genetic
information between bacteria. It is the direct transfer of bacterial DNA between
organisms and requires CELL-TO-CELL CONTACT.

Most conjugation is PLASMID- MEDIATED.

PLASMIDS are small, circular, ds-DNA molecules, measuring about 2-10 kilobases.
They all have the ability to replicate in bacteria. Multiple copies of plasmids can be
found in a host cell, however, not all plasmids can transfer themselves.

A CONJUGATIVE plasmid has the codes for the genes allowing transfer between
cells.

A NONCONJUGATIVE plasmid requires the help of a conjugative plasmid to transmit


itself to another bacteria.

NARROW-HOST RANGE PLASMIDS exist only within a single species.

BROAD-HOST RANGE PLASMIDS exist in different genera of organisms.

Plasmids contain the following:

1. And ORI or origin of replication that allows autonomous replication

2. One or more genes that confer specific antibiotic resistance

3. Sites for restriction endonucleases which are used for inserting specific DNA
fragments.

4. Encode for virulence factors such as toxins or adhesins

Plasmids capable of mediating conjugation carry genes coding for the production of
a PILUS, a protein appendage which is found on the surface of the donor cells.
Bacteria which carry these plasmids are called F(+) cells, making them the donor
cells The tip of the pilus attaches to the surface of the recipient cell, and DNA
transfer occurs. It is unsure whether the DNA is transferred through the pilus, or if
the pilus serves as the mechanism for holding the two cells together. In either way,
this is the reason why it has also been termed BACTERIAL SEX.

Once the recipient cell acquires the F plasmid, it changes from a F(-) to a F (+) cell,
thus allowing it to become a donor cell now.

In a very small proportion of cells, the F plasmid is incorporated into the bacterial
chromosome. Once inserted, the entire bacterial chromosome acts like a F plasmid.
These are then called high frequency recombination strains (Hfr strains). This leads
to 2 processes:

1. The F plasmid and the entire bacterial DNA undergoes conjugation with an
F(-) cell.
2. The F plasmid is excised along with a portion of the bacterial DNA, hence
the F plasmid is termed F prime (F’)

For instance, the F-lac plasmid is an F’ plasmid carrying carrying the genes for E.coli
lactose operon. When this plasmid is transferred to non-lactose formers, they are
converted to lactose fermenters.

Note that the F plasmid is confined to the Escherichia genus and other closely
related enteric bacteria.

THE GENETIC BASIS OF ANTIBIOTIC RESISTANCE

Thus, all of the properties of a micro-organism are determined by the genes located
either on the chromosome, on the plasmid or on the bacteriophage.

There are 2 types of resistance:

1. Intrinsic resistance

Organisms that are a naturally insensitive to a particular drug will always exit.
The most obvious determinant of bacterial response to an antibiotic is the
presence or absence of the target for the action of the drug.

For example: Antifungals like amphotericin B bind to the sterols on the fungal
cell wall, altering it permeability, thus killing it. However, bacterial cells do
not have sterols, hence they have an intrinsic resistance to the drug.

2. Acquired resistance.

Three main factors affect the frequency of acquired resistance:

A. The amount of antibiotic being used


B. The frequency with which bacteria can undergo spontaneous mutations to
reistance
C. The prevalence of plasmids able to transfer resistance from one bacterium
to another.

How does it happen?

1. Chromosomal mutations

Random spontaneous mutations occur continuously at a low frequency in


all bacterial populations. These mutations may allow the resistant mutant
to survive, grow and eventually become the predominant or only member
of the population.
Mutation can occur by base substitution, that is, one base is inserted in
place of another. It takes place at the time of DNA replication. When the
base substitution results in a codon that simply causes a different amino
acid to be inserted, the mutation is called a MISSENSE mutation. When the
base substitution generates a termination codon that stops protein
synthesis prematurely, this is called a NONSENSE mutation.

Mutation may also occur by FRAMESHIFT; this occurs when one or more
base pairs are added or deleted, which in turn shifts the reading frame on
the ribosome, resulting in a wrong amino acid, and the production of a n
inactive protein.

In SINGLE LARGE –STEP MUTATIONS, the cell is able to carry out its
biological functions but the drug target site is altered. If some affinity is
still present to the drug, increasing the minimum inhibitory concentration
may cause an effect on the bacteria.

In MULTISTEP PATTERN OF RESISTANCE, the development of resistance


starts slightly, then with each new mutant formed , the resistance
increase, eventually leading to an organism which is highly resistant. This
is the principle behind the multidrug therapy for pulmonary tuberculosis.
Each drug kills the few mutants that are resistant to the other.

Another example of chromosomal mutation leading to antibiotic resistance


are those involving Beta-lactamases. These are enzymes produced by
Staphylococcus organisms, act on the Beta-lactam ring in penicillins and
cephalosporins. Mutations in the genes that encode for these enzymes
can cause an overproduction, leading to drug resistance.

Another is the resistance gene mecA which coded for a unique penicillin-
binding protein found in methicillin-resistant S.aureus. It renders the
bacteria resistant to B-lactam drugs, as well as aminoglycoside and
quinolones. Thus, vancomycin is used, acting on cell wall synthesis.

2. Plasmid- mediated conjugation

Of the 3 modes of gene transfer, this is of greatest importance with


regards to drug resistance.

R PLASMIDS are plasmids which confer resistance to one or more


unrelated groups of antibiotics.

They were first demonstrated in Japan in the 1950’s, where resistance to


several antibiotics could be transferred by conjugation between strains of
Shigella and E.coli.
This is further accelerated by the presence of TRANSPOSONS. A
transposon consists of two insertion sequences flanking another gene or
set of genes. The Insertion sequences are small pieces of DNA that code
for the enzyme TRANSPOSASE, which allow them to jmpin and out of DNA.
Meaning, the transposons can insert themselves into a donor chromosome
WITHOUT having DNA homology. They do not replicate independently, but
are copied as their host’s DNA is transcribed.

The ability of the transposon to confer resistance is by the presence of


INTEGRON, the building block of all transposons, and it allows the rapid
formation and expression of new combinations of antibiotic resistance
genes.

The ability to move between chromosomes, plasmids or bacteriophages is


an efficient method for moving genes in a bacterial population. Thus, they
are frequently associated with the formation of multiple-drug resistant
plasmids.

This is the most common cause of drug resistance in Gram(-) bacteria.

Self study: LANDMARKS IN GENETICS, DNA STRUCTURE AND CHROMOSOME


FUNCTION chapter 11, pages 75 -78