LabAid

PageRuler Plus Prestained Protein Ladder (10 250 kDa)

Migration patterns of PageRuler Plus Prestained Protein Ladder in different electrophoresis conditions
Gel type Gel concentration 4-20% Running buffer Tris-Glycine 8-16% 10-20% 8% Tris-Glycine 250 130 100 70 10% 12% 15% Tris-Acetate* 3-8% 7% 4-12% MOPS MES Tris-Acetate Bis-Tris* 10% MOPS MES 12% MOPS MES

LabAid
PageRuler Plus Prestained Protein Ladder (10 250 kDa)
PageRuler Plus Prestained Protein Ladder, #SM1811
kDa ~250 ~130 185 115 80 65 50 30 50 30 25 15 10 15

10 20 30 % lenght of gel 40 50 60 70 80 90 100 10 250 130 100 70 55 35 25 15 35 25 15 10 250 130 100 70 55

250 130 100 70 55 35 25

250 130 100 70 55

250 130 100 70 55 35 25

Apparent Molecular Weights, kDa 250 130 100 205 70 205 55 55 120 35 25 35 25 120 85 65 15 15 50 85 65

190 115 80 70 50

185 115 80 65 50

190 115 80 70 50 30

185 115 80 65 50

190 115 80 70 50

~100 ~70 ~55 ~35

Features Sharp bands Stable colors Bright reference bands Broad range Ready-to-use supplied in a loading buffer for direct loading on gels Applications Monitoring protein migration during SDS-polyacrylamide gel electrophoresis Monitoring protein transfer onto membranes after Western blotting Sizing of proteins on SDS-polyacrylamide gels and Western blots

Recommendations for Loading:

Step1: Thaw the Ladder either at room temperature or at 37C for a few minutes to dissolve precipitated solids. Step2: Mix gently, but throughly, to ensure that the solution is homogeneous. Step3: Load on SDS-polyacrylamide gel for mini-gels with a thickness of 0.75-1 mm: 5 l/well* for large gels with a thickness of 0.75-1 mm: 10 l/well* * For thicker gels, the loading volume should be increased.

30 25 30 25 15 Gel

~25 ~15 ~10 Blot 4-20% Tris-glycine SDS-PAGE

30 25

35 15 25

25 15 10 15

25

15 15 10 10

10

10

30 25

30 25

15 10

Applications Product PageRuler Plus Prestained Protein Ladder Catalog # SM1811 Amount 2x250 l mini-gels 5 l/well 100 large gels 10 l/well 50

* Migration patterns were determined using respective NuPAGE precast gels. Note. The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis conditions.

MW range, PageBlue staining PageSilver staining kDa (#R0571) (#K0681) 10-250 compatible compatible

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L6P

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