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Central University of Rajasthan

CENTRAL UNIVERSITY OF RAJASTHAN

Central University of Rajasthan CENTRAL UNIVERSITY OF RAJASTHAN Laboratory Safety Training Dr. Janmejay Pandey

Laboratory Safety Training

Dr. Janmejay Pandey

Central University of Rajasthan

CENTRAL UNIVERSITY OF RAJASTHAN

Outline of the presentation:

1.

Why is it important

2.

Some fundamental Concepts

3.

Safety in Chemical & Biological Labs

4.

Safety Supplies and Equipments

5.

Safe Laboratory Practices

6.

Safety from fire

7.

Your contribution towards making labs safe

CENTRAL UNIVERSITY OF RAJASTHAN Why is it important ? C U Rajasthan- Health & Environmental

CENTRAL UNIVERSITY OF RAJASTHAN

Why is it important ?

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CENTRAL UNIVERSITY OF RAJASTHAN C. U. Rajasthan- Motto Education for sustainable Development Laboratory Safety !

CENTRAL UNIVERSITY OF RAJASTHAN

C. U. Rajasthan- Motto

Education for sustainable Development

Laboratory Safety !

A critical component of sustainable development

CENTRAL UNIVERSITY OF RAJASTHAN Consequences of lack of lab safety measures ! 1. Damage or

CENTRAL UNIVERSITY OF RAJASTHAN

Consequences of lack of lab safety measures !

1. Damage or loss of institutional property/ valuables.

2. Loss of important work/ results.

3. Most importantly

disabilities.

Serious personal injuries, illness and

importantly disabilities. … Serious personal injuries, illness and C U Rajasthan- Health & Environmental Safety 5
importantly disabilities. … Serious personal injuries, illness and C U Rajasthan- Health & Environmental Safety 5

C U Rajasthan- Health & Environmental Safety

importantly disabilities. … Serious personal injuries, illness and C U Rajasthan- Health & Environmental Safety 5

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CENTRAL UNIVERSITY OF RAJASTHAN Negligence of lab safety : A horror story Sheri Sangji 1.

CENTRAL UNIVERSITY OF RAJASTHAN

Negligence of lab safety : A horror story

OF RAJASTHAN Negligence of lab safety : A horror story Sheri Sangji 1. UCLA (Dec. 18,

Sheri Sangji

Negligence of lab safety : A horror story Sheri Sangji 1. UCLA (Dec. 18, 2008). A

1. UCLA (Dec. 18, 2008). A chemistry researcher working with a pyrophoric chemical got caught in a Laboratory fire.

2. She suffered deep 3 rd degree burns over 40 % of her body & 18 days later she died from this accident.

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CENTRAL UNIVERSITY OF RAJASTHAN Lack of lab safety training : Another tragic example

1. Georgia Institute of Technology (2003). An Afro- American

Lab Technician attempted to bring down a non- labeled

‘reagent bottle’ from a high shelf.

2. The bottle slipped out of her hand, content of the bottle spilled over her. It turned out to be concentrated acid.

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CENTRAL UNIVERSITY OF RAJASTHAN

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CENTRAL UNIVERSITY OF RAJASTHAN Do we need to be in accidents to learn lessons !

CENTRAL UNIVERSITY OF RAJASTHAN

CENTRAL UNIVERSITY OF RAJASTHAN Do we need to be in accidents to learn lessons !
CENTRAL UNIVERSITY OF RAJASTHAN Do we need to be in accidents to learn lessons !

Do we need to be in accidents to learn lessons !

CENTRAL UNIVERSITY OF RAJASTHAN Most frequently reported infections in Laboratory workers during last 10 years

CENTRAL UNIVERSITY OF RAJASTHAN

Most frequently reported infections in Laboratory workers

during last 10 years

Disease or Agent

No. of Cases

Mycobacterium tuberculosis

223

Q fever

176

Hantavirus

169

Hepatitis B virus

84

Brucella sp.

81

Salmonella sp.

66

Shigella sp.

56

Hepatitis non-A, non-B

28

Cryptosporidium sp.

27

Total

1074

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CENTRAL UNIVERSITY OF RAJASTHAN Flying laboratory instruments/ objects C U Rajasthan- Health & Environmental Safety

CENTRAL UNIVERSITY OF RAJASTHAN

Flying laboratory instruments/ objects

CENTRAL UNIVERSITY OF RAJASTHAN Flying laboratory instruments/ objects C U Rajasthan- Health & Environmental Safety 11
CENTRAL UNIVERSITY OF RAJASTHAN Flying laboratory instruments/ objects C U Rajasthan- Health & Environmental Safety 11

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CENTRAL UNIVERSITY OF RAJASTHAN Injuries to Research Students/ Employee Source: Howard Hughes Medical Institute, Office

CENTRAL UNIVERSITY OF RAJASTHAN

Injuries to Research Students/ Employee

OF RAJASTHAN Injuries to Research Students/ Employee Source: Howard Hughes Medical Institute, Office of

Source: Howard Hughes Medical Institute, Office of Laboratory Safety

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CENTRAL UNIVERSITY OF RAJASTHAN Injuries to Laboratory Support Staff/ Random Visitor Source: Howard Hughes Medical

CENTRAL UNIVERSITY OF RAJASTHAN

Injuries to Laboratory Support Staff/ Random Visitor

Injuries to Laboratory Support Staff/ Random Visitor Source: Howard Hughes Medical Institute, Office of

Source: Howard Hughes Medical Institute, Office of Laboratory Safety

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CENTRAL UNIVERSITY OF RAJASTHAN Government Regulations for Lab Safety Training is an important government regulation

CENTRAL UNIVERSITY OF RAJASTHAN

Government Regulations for Lab Safety

Training is an important government regulation in western world

1. Regulatory requirement in several developed/ developing

countries and organizations e.g. (NIH, CDC, OSHA, EPA-

USA), (CSIRO- Aus), (EMBL- EU).

2. Institutional and Laboratory quality assessment & audit.

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CENTRAL UNIVERSITY OF RAJASTHAN Indian Status C U Rajasthan- Health & Environmental Safety 15

CENTRAL UNIVERSITY OF RAJASTHAN

Indian Status

CENTRAL UNIVERSITY OF RAJASTHAN Indian Status C U Rajasthan- Health & Environmental Safety 15

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CENTRAL UNIVERSITY OF RAJASTHAN • We need to learn the risks associated with labs in

CENTRAL UNIVERSITY OF RAJASTHAN

We need to learn the risks associated with labs in a holistic manner & also how to mitigate their hazardous effects.

How can we make our labs safe !

Lab Safety Measures

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CENTRAL UNIVERSITY OF RAJASTHAN Key Concepts of Laboratory Safety Laboratory safety revolves around understanding, safe

CENTRAL UNIVERSITY OF RAJASTHAN

Key Concepts of Laboratory Safety

Laboratory safety revolves around

understanding, safe storage, usage & disposal of

hazardous components of lab work

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CENTRAL UNIVERSITY OF RAJASTHAN Key Concepts of Laboratory Safety 1. Understand 2. Organize 3. Ensure

CENTRAL UNIVERSITY OF RAJASTHAN

Key Concepts of Laboratory Safety

1. Understand

2. Organize

3. Ensure containment

4. Protect

5. Be prepared

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CENTRAL UNIVERSITY OF RAJASTHAN 1. Understand Prior preparation is extremely useful in ensuring lab safety.

CENTRAL UNIVERSITY OF RAJASTHAN

1. Understand

Prior preparation is extremely useful in ensuring lab safety.

Physical:

Chemical:

Biological:

Specific:

Get training for safe use of instrument.

Use MSDS and other source about chemicals.

Read about hazardous effects of biological material.

Get necessary specific training e.g. radiological/ laser etc.

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CENTRAL UNIVERSITY OF RAJASTHAN 2. Organize • Organize your work and work place before you

CENTRAL UNIVERSITY OF RAJASTHAN

2. Organize

Organize your work and work place before you start.

Work diligently.

Be alert and attentive.

Don’t work in lab in haste.

Ensure everything is safe before leaving the lab.

(Most laboratory accidents happen when people are distracted or hurried) Clinical Microbiol. Rev. (1995). 8: 389- 405

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CENTRAL UNIVERSITY OF RAJASTHAN 3. Ensure containment • Keep hazardous material (chemicals, biological and

CENTRAL UNIVERSITY OF RAJASTHAN

3. Ensure containment

Keep hazardous material (chemicals, biological and

physical hazards) within at least 2 levels of containments.

Contain any undesired spillage/ release of any and all hazardous material.

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CENTRAL UNIVERSITY OF RAJASTHAN 4. Protect (yourself and co-workers) Use appropriate protective and safety equipments.

CENTRAL UNIVERSITY OF RAJASTHAN

4. Protect (yourself and co-workers)

Use appropriate protective and safety equipments.

1.

Protective clothing (Lab cloth, Lab glasses, Gloves, Rubber Boots etc.).

2.

Chemical Fume Hood.

3.

Laminar Air Flow and Bio-Safety Cabinets.

4.

Protective Working Shield.

CENTRAL UNIVERSITY OF RAJASTHAN 5. Be prepared (for emergencies) 1. Despite all the laboratory safety

CENTRAL UNIVERSITY OF RAJASTHAN

5. Be prepared (for emergencies)

1.

Despite all the laboratory safety trainings, you may still encounter laboratory accidents

2.

We must be adequately trained and prepared for such

events.

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CENTRAL UNIVERSITY OF RAJASTHAN Safety in Chemical & Biological Labs C U Rajasthan- Health &

CENTRAL UNIVERSITY OF RAJASTHAN

Safety in Chemical & Biological Labs

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CENTRAL UNIVERSITY OF RAJASTHAN Chemical hazards in a laboratory Several forms of chemical hazards C

CENTRAL UNIVERSITY OF RAJASTHAN

Chemical hazards in a laboratory

UNIVERSITY OF RAJASTHAN Chemical hazards in a laboratory Several forms of chemical hazards C U Rajasthan-

Several forms of chemical hazards

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CENTRAL UNIVERSITY OF RAJASTHAN Explosive Any chemical (solid, liquid or gas) that is in its

CENTRAL UNIVERSITY OF RAJASTHAN

Explosive

CENTRAL UNIVERSITY OF RAJASTHAN Explosive Any chemical (solid, liquid or gas) that is in its unstable

Any chemical (solid, liquid or gas) that is in its unstable equilibrium.CENTRAL UNIVERSITY OF RAJASTHAN Explosive When initiated by shock they undergo rapid chemical change Have the

When initiated by shock they undergo rapid chemical change(solid, liquid or gas) that is in its unstable equilibrium. Have the tendency to change into

Have the tendency to change into more stable compound (usually gas)When initiated by shock they undergo rapid chemical change In the process of change they give

In the process of change they give out energy, heat, light & soundHave the tendency to change into more stable compound (usually gas) C U Rajasthan- Health &

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CENTRAL UNIVERSITY OF RAJASTHAN Characteristics of explosive • Can expand upto 10,000 – 15,000 times

CENTRAL UNIVERSITY OF RAJASTHAN

CENTRAL UNIVERSITY OF RAJASTHAN Characteristics of explosive • Can expand upto 10,000 – 15,000 times of

Characteristics of explosive

Can expand upto 10,000 15,000 times of their original volume

Expansion may occur at a rate of 6,000 meters/ second

Exert pressure of 110 metric ton/ square centimeter

Cause temperature of 3,000- 4,000 o C within 10 seconds

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CENTRAL UNIVERSITY OF RAJASTHAN Corrosive • Corrosive chemicals (Acids, Bases, Strong Oxidizing Agent) • Corrosive

CENTRAL UNIVERSITY OF RAJASTHAN

Corrosive

CENTRAL UNIVERSITY OF RAJASTHAN Corrosive • Corrosive chemicals (Acids, Bases, Strong Oxidizing Agent) • Corrosive

Corrosive chemicals (Acids, Bases, Strong Oxidizing Agent)

Corrosive chemicals react with animal tissues & metals

Cause burn and permanent tissue damage at the point of contact

Corrosives can cause irreversible damage to eyes

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CENTRAL UNIVERSITY OF RAJASTHAN Corrosive Protection • Work in Chemical Fume Hood, Protect Eye, Face,

CENTRAL UNIVERSITY OF RAJASTHAN

Corrosive Protection

CENTRAL UNIVERSITY OF RAJASTHAN Corrosive Protection • Work in Chemical Fume Hood, Protect Eye, Face, Skin

Work in Chemical Fume Hood, Protect Eye, Face, Skin

PVC and nitrile gloves often provide appropriate protection

Use splash goggles, face shield if splashing is possible

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CENTRAL UNIVERSITY OF RAJASTHAN (A- W of dilution) : Add Acid gently to Water 1.

CENTRAL UNIVERSITY OF RAJASTHAN

(A- W of dilution) :

Add Acid gently to Water

OF RAJASTHAN (A- W of dilution) : Add Acid gently to Water 1. Make sure you

1.

Make sure you add a very small amount of the acid to the water.

2.

Never add the water to a concentrated acid solution.

3.

The Acid- Water mixing is an exothermic reaction and gives off loads of heat.

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CENTRAL UNIVERSITY OF RAJASTHAN Oxidizer • Oxidizers are not of themselves combustible, but can initiate

CENTRAL UNIVERSITY OF RAJASTHAN

Oxidizer

CENTRAL UNIVERSITY OF RAJASTHAN Oxidizer • Oxidizers are not of themselves combustible, but can initiate and

Oxidizers are not of themselves combustible, but can initiate and support combustion

These are chemicals capable of supporting a fire by giving oxygen to the fire.

Examples: Organic Peroxide with basic chemical structure R-O-O-R.

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CENTRAL UNIVERSITY OF RAJASTHAN Handling Procedures for Oxidizers • Always handle in a chemical fume

CENTRAL UNIVERSITY OF RAJASTHAN

Handling Procedures for Oxidizers

UNIVERSITY OF RAJASTHAN Handling Procedures for Oxidizers • Always handle in a chemical fume hood. •

Always handle in a chemical fume hood.

Wear safety goggles, a lab coat, and appropriate gloves.

Use fire blankets to deprive fires of oxygen

Shut sash on fume hood fires

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CENTRAL UNIVERSITY OF RAJASTHAN Flammables Flammable Liquid • Any liquid that has a flash point

CENTRAL UNIVERSITY OF RAJASTHAN

Flammables

CENTRAL UNIVERSITY OF RAJASTHAN Flammables Flammable Liquid • Any liquid that has a flash point below

Flammable Liquid

Any liquid that has a flash point below 100 o F

Differs from combustible liquid (Any liquid that has a flash point

at or above 100 o F)

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CENTRAL UNIVERSITY OF RAJASTHAN Flammables Flammable Solids • Any solid that catches fire through friction

CENTRAL UNIVERSITY OF RAJASTHAN

Flammables

CENTRAL UNIVERSITY OF RAJASTHAN Flammables Flammable Solids • Any solid that catches fire through friction or

Flammable Solids

Any solid that catches fire through friction or which could be

readily ignited.

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CENTRAL UNIVERSITY OF RAJASTHAN While working with flammables • Remove Ignition Sources (heat or spark)

CENTRAL UNIVERSITY OF RAJASTHAN

While working with flammables

CENTRAL UNIVERSITY OF RAJASTHAN While working with flammables • Remove Ignition Sources (heat or spark) •

Remove Ignition Sources (heat or spark)

Keep away from oxidizers and combustible materials

Work in a fume hood or well ventilated area

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CENTRAL UNIVERSITY OF RAJASTHAN Reactive chemicals • Reactive materials can release heat or a toxic

CENTRAL UNIVERSITY OF RAJASTHAN

Reactive chemicals

CENTRAL UNIVERSITY OF RAJASTHAN Reactive chemicals • Reactive materials can release heat or a toxic or

Reactive materials can release heat or a toxic or flammable gas upon contact with water.

Safe handling of these materials will depend on the specific materials and the conditions in which this material is handled.

Examples (Sodium metal, Lithium aluminum hydride)

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CENTRAL UNIVERSITY OF RAJASTHAN Pyrophoric materials • Pyrophoric materials can react with air and ignite

CENTRAL UNIVERSITY OF RAJASTHAN

Pyrophoric materials

CENTRAL UNIVERSITY OF RAJASTHAN Pyrophoric materials • Pyrophoric materials can react with air and ignite

Pyrophoric materials can react with air and ignite spontaneously at or below 113°F.

inert

Pyrophoric

materials

should

be

handled

and

stored

in

environments.

Examples: (Silane, White or yellow phosphorous, t-butyl lithium)

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CENTRAL UNIVERSITY OF RAJASTHAN Poison/ Toxin • Cause illness or injury by upsetting biological functions

CENTRAL UNIVERSITY OF RAJASTHAN

Poison/ Toxin

CENTRAL UNIVERSITY OF RAJASTHAN Poison/ Toxin • Cause illness or injury by upsetting biological functions or

Cause illness or injury by upsetting biological functions or

damaging biological structures

Dose-response relationship

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CENTRAL UNIVERSITY OF RAJASTHAN Working with Toxins/ Poison • Read MSDS for exposure limits and

CENTRAL UNIVERSITY OF RAJASTHAN

Working with Toxins/ Poison

CENTRAL UNIVERSITY OF RAJASTHAN Working with Toxins/ Poison • Read MSDS for exposure limits and proper

Read MSDS for exposure limits and proper handling information

Use Fume Hood

Minimize exposure by using proper protective equipments

No food or drinks in lab

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CENTRAL UNIVERSITY OF RAJASTHAN • Carcinogenic chemicals • Cause abnormal growth of tissue • May

CENTRAL UNIVERSITY OF RAJASTHAN

Carcinogenic chemicals

CENTRAL UNIVERSITY OF RAJASTHAN • Carcinogenic chemicals • Cause abnormal growth of tissue • May have
CENTRAL UNIVERSITY OF RAJASTHAN • Carcinogenic chemicals • Cause abnormal growth of tissue • May have

Cause abnormal growth of tissue

May have variety of different mechanism

Should be handled only after appropriate training

Ethidium Bromide, Aromatic Pesticides, etc.

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CENTRAL UNIVERSITY OF RAJASTHAN Radioactive 1. Radioactive atoms with unstable particles nucleus loses energy by

CENTRAL UNIVERSITY OF RAJASTHAN

Radioactive

CENTRAL UNIVERSITY OF RAJASTHAN Radioactive 1. Radioactive atoms with unstable particles nucleus loses energy by emitting
CENTRAL UNIVERSITY OF RAJASTHAN Radioactive 1. Radioactive atoms with unstable particles nucleus loses energy by emitting

1. Radioactive atoms with unstable particles

nucleus loses energy by emitting ionizing

2. Several applications in laboratory

3. Extremely harmful, know n carcinogen

4. Should be used only after detailed training

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CENTRAL UNIVERSITY OF RAJASTHAN Understand the lab chemical and its potential hazard Manufacturer Identity Hazards

CENTRAL UNIVERSITY OF RAJASTHAN

Understand the lab chemical and its potential hazard

ManufacturerUnderstand the lab chemical and its potential hazard Identity Hazards ? C U Rajasthan- Health &

Identitythe lab chemical and its potential hazard Manufacturer Hazards ? C U Rajasthan- Health & Environmental

Hazardsthe lab chemical and its potential hazard Manufacturer Identity ? C U Rajasthan- Health & Environmental

lab chemical and its potential hazard Manufacturer Identity Hazards ? C U Rajasthan- Health & Environmental
lab chemical and its potential hazard Manufacturer Identity Hazards ? C U Rajasthan- Health & Environmental

?

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CENTRAL UNIVERSITY OF RAJASTHAN National Fire Protection Association (NFPA) Explanation Guide for Chemical hazard C

CENTRAL UNIVERSITY OF RAJASTHAN

National Fire Protection Association (NFPA) Explanation Guide for Chemical hazard

Protection Association (NFPA) Explanation Guide for Chemical hazard C U Rajasthan- Health & Environmental Safety 43

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CENTRAL UNIVERSITY OF RAJASTHAN MSDS Information Material Safety Data Sheet provides comprehensive information about: •

CENTRAL UNIVERSITY OF RAJASTHAN

MSDS Information

Material Safety Data Sheet provides

comprehensive information about:

Physical Properties

Hazards

First Aid measures

Engineering controls

PPE

• First Aid measures • Engineering controls • PPE Must have a copy of MSDS for

Must have a copy of MSDS for each chemical in the laboratory for easy access in an emergency

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CENTRAL UNIVERSITY OF RAJASTHAN Why MSDS Information important ?   MSDS Contents   1. Identification

CENTRAL UNIVERSITY OF RAJASTHAN

Why MSDS Information important ?

 

MSDS Contents

 

1. Identification of substance

9. Physical and chemical properties

2. Composition/Data on components

10. Stability and reactivity

3. Hazards identification

11. Toxicological information

4. First aid measures

12. Ecological information

5. Fire fighting measures

13. Disposal considerations

6. Spill cleanup measures

14. Transport information

7. Handling and storage

15. Regulations

8. Exposure controls and personal protection

16. Other relevant information

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CENTRAL UNIVERSITY OF RAJASTHAN Guidelines for safe storage of laboratory chemical Must be followed to:

CENTRAL UNIVERSITY OF RAJASTHAN

Guidelines for safe storage of laboratory chemical

Must be followed to:

1. Prevent

unwanted

being mixed

reactions

e.g.

incompatible

chemicals

2. Such reactions may generate heat/ fume/ gases that can lead to explosion/ fire and cause damage to personnel and property.

3. Preventing small accident from turning into major disaster

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CENTRAL UNIVERSITY OF RAJASTHAN Chemical Storage Guidelines • The storage area and cabinets should be

CENTRAL UNIVERSITY OF RAJASTHAN

Chemical Storage Guidelines

The storage area and cabinets should be labeled to identify

the hazardous nature of the products stored within

identify the hazardous nature of the products stored within Ensure that all chemicals are stored according
identify the hazardous nature of the products stored within Ensure that all chemicals are stored according

Ensure that all chemicals are stored according to compatibility (e.g., oxidizers away from flammable liquids).

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CENTRAL UNIVERSITY OF RAJASTHAN Flammables storage 1. Flammables include organic liquids, combustibles, and organic acids

CENTRAL UNIVERSITY OF RAJASTHAN

Flammables storage

1. Flammables include organic liquids, combustibles,

and organic acids etc.

2. Flammable materials kept outside a cabinet should be in fire proof safety cans.

3. Flammable

inside

liquids

(>1L)

must

be

stored

flammable cabinets.

4. Never store flammable liquids in a standard cabinet. Example: i.e. acetone, hexane, acetic acid, phenol, and chloroform

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CENTRAL UNIVERSITY OF RAJASTHAN Solvent storage

1.

Solvents should be stored in well organized dedicated storage cabinet

2.

Read MSDS carefully to determine compatibility before storage

2. Read MSDS carefully to determine compatibility before storage C U Rajasthan- Health & Environmental Safety

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CENTRAL UNIVERSITY OF RAJASTHAN Acids Storage • Store by acid class in separate secondary containment

CENTRAL UNIVERSITY OF RAJASTHAN

Acids Storage

Store by acid class in separate secondary containment

Never store acids above eye level

secondary containment • Never store acids above eye level • Concentrated per-chloric acid should be stored

Concentrated per-chloric acid should be stored alone in a cabinet by itself

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CENTRAL UNIVERSITY OF RAJASTHAN Bases Storage • Store in secondary containment • Store away from

CENTRAL UNIVERSITY OF RAJASTHAN

Bases Storage

Store in secondary containment

Store away from acids and solvents

Never store at or above eye level.

Examples:

Hydroxides

Amines

Ammonia

Bleach

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CENTRAL UNIVERSITY OF RAJASTHAN • Dry shelves. non-hazardous chemicals can be stored on laboratory C

CENTRAL UNIVERSITY OF RAJASTHAN

Dry

shelves.

non-hazardous

chemicals

can

be

stored

on

laboratory

• Dry shelves. non-hazardous chemicals can be stored on laboratory C U Rajasthan- Health & Environmental

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CENTRAL UNIVERSITY OF RAJASTHAN • Chemicals should never be stored on the floor. • If

CENTRAL UNIVERSITY OF RAJASTHAN

Chemicals should never be stored on the floor.

If need be they must be stored in approved secondary containers.

• If need be they must be stored in approved secondary containers. C U Rajasthan- Health

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CENTRAL UNIVERSITY OF RAJASTHAN Compressed Gases Compressed gases may pose a physical and/or health hazard

CENTRAL UNIVERSITY OF RAJASTHAN

Compressed Gases

Compressed gases may pose a physical and/or health hazard depending on the gas being used.

Restraint:

Gas cylinders must be individually

secured with suitable regulators

Only cylinders with safety caps can be group chained.

regulators • Only cylinders with safety caps can be group chained. C U Rajasthan- Health &
regulators • Only cylinders with safety caps can be group chained. C U Rajasthan- Health &

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CENTRAL UNIVERSITY OF RAJASTHAN Storing compressed gases

Flammable gases (i.e. propane, hydrogen) must be stored away from sources of ignition and oxidizing gases (i.e. oxygen).

Poison/toxic gases (i.e. chlorine, carbon monoxide, phosgene or

ammonia) can pose serious potential hazards to personnel and therefore special storage and handling measures are required.

Poison gases must be stored as follows:

Small size cylinders (fit inside hood) Use and storage in hood Large size cylinders (regular use) Use and store in ventilated

with an air monitoring and alarm system

C U Rajasthan- Health & Environmental Safety

Use and store in ventilated with an air monitoring and alarm system C U Rajasthan- Health

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CENTRAL UNIVERSITY OF RAJASTHAN Design and working principles of Fume Hoods C U Rajasthan- Health

CENTRAL UNIVERSITY OF RAJASTHAN

Design and working principles of Fume Hoods

UNIVERSITY OF RAJASTHAN Design and working principles of Fume Hoods C U Rajasthan- Health & Environmental

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CENTRAL UNIVERSITY OF RAJASTHAN Using Fume Hoods • Use For All Operations Where Odoriferous, Volatile,

CENTRAL UNIVERSITY OF RAJASTHAN

Using Fume Hoods

Use For All Operations Where Odoriferous, Volatile, Toxic or Harmful Release Possible

When using the fume hood you first make sure that the exhaust

blower is operating and air is entering the hood.

Remember, do not put your face inside the hood

Work at least 6 inches into the hood

Minimize storage of chemicals in the hood

Clean spills immediately

Work with the sash at the proper operating level as guided by the indicator arrows

sash at the proper operating level as guided by the indicator arrows C U Rajasthan- Health
sash at the proper operating level as guided by the indicator arrows C U Rajasthan- Health

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CENTRAL UNIVERSITY OF RAJASTHAN Testing Fume Hood Functionality 1. Functional testing of the Fume Hoods

CENTRAL UNIVERSITY OF RAJASTHAN

Testing Fume Hood Functionality

UNIVERSITY OF RAJASTHAN Testing Fume Hood Functionality 1. Functional testing of the Fume Hoods is done
UNIVERSITY OF RAJASTHAN Testing Fume Hood Functionality 1. Functional testing of the Fume Hoods is done

1. Functional testing of the Fume Hoods is done with Dry Ice

2. Fume Hoods with unidirectional blow of dry ice away from the sash

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CENTRAL UNIVERSITY OF RAJASTHAN Biological Hazards in a laboratory Several forms of biological hazards C

CENTRAL UNIVERSITY OF RAJASTHAN

Biological Hazards in a laboratory

UNIVERSITY OF RAJASTHAN Biological Hazards in a laboratory Several forms of biological hazards C U Rajasthan-

Several forms of biological hazards

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CENTRAL UNIVERSITY OF RAJASTHAN Before you use any biological sample in the laboratory Learn about

CENTRAL UNIVERSITY OF RAJASTHAN

Before you use any biological sample in the laboratory

Learn about the potential risk associated with any biological sample before using it in your lab experiment

(Sources for information)

Center for Disease Control (CDC)

Food & Drug Administration (FDA)

American Type Culture Collection (ATCC)

German Culture Collection (DSMZ)

Microbial Type Culture Collection (MTCC)

C U Rajasthan- Health & Environmental Safety

60

CENTRAL UNIVERSITY OF RAJASTHAN Information about the bio-safety level of biological sample C U Rajasthan-

CENTRAL UNIVERSITY OF RAJASTHAN

Information about the bio-safety level of biological sample

C U Rajasthan- Health & Environmental Safety
C U Rajasthan- Health & Environmental
Safety

61

CENTRAL UNIVERSITY OF RAJASTHAN Aerosols and Droplets Aerosols and Droplets are the main sources of

CENTRAL UNIVERSITY OF RAJASTHAN

Aerosols and Droplets

Aerosols and Droplets are the main sources of biohazard

contamination

Aerosols and Droplets are the main sources of biohazard contamination C U Rajasthan- Health & Environmental

C U Rajasthan- Health & Environmental Safety

62

CENTRAL UNIVERSITY OF RAJASTHAN Reduce Aerosols • Gently expel fluids against the walls of tubes
CENTRAL UNIVERSITY OF RAJASTHAN Reduce Aerosols • Gently expel fluids against the walls of tubes

CENTRAL UNIVERSITY OF RAJASTHAN

CENTRAL UNIVERSITY OF RAJASTHAN Reduce Aerosols • Gently expel fluids against the walls of tubes or

Reduce Aerosols

CENTRAL UNIVERSITY OF RAJASTHAN Reduce Aerosols • Gently expel fluids against the walls of tubes or

Gently expel fluids against the walls of tubes or flasks

Perform aerosol forming experiments in Biological Safety Cabinet

C U Rajasthan- Health & Environmental Safety

63

CENTRAL UNIVERSITY OF RAJASTHAN Bio Safety Level I • Hazard group 1- P1 • Doors

CENTRAL UNIVERSITY OF RAJASTHAN

Bio Safety Level I

Hazard group 1- P1

Doors closed during work

Normal laboratory procedures

C U Rajasthan- Health & Environmental Safety 64
C U Rajasthan- Health & Environmental
Safety
64
CENTRAL UNIVERSITY OF RAJASTHAN Containment level 2 • Hazard group 1 – 2 (P1- P2)

CENTRAL UNIVERSITY OF RAJASTHAN

Containment level 2

Hazard group 1 2 (P1- P2)

Use Class II safety cabinet

Use autoclave

• Protect against aerosol generation Class II Auto
• Protect against aerosol generation
Class II
Auto

C U Rajasthan- Health & Environmental Safety

65

CENTRAL UNIVERSITY OF RAJASTHAN Bio Safety Level III • Hazard group 3 P1 -P3 •

CENTRAL UNIVERSITY OF RAJASTHAN

Bio Safety Level III

Hazard group 3 P1 -P3

Keep Laboratory and changing room at negative pressure so air flows from outdoors to indoors

Use Class II safety cabinet

Entry

Entry
Entry
Entry

Change room

( ve)
(
ve)
Entry Change room ( ve) Class II Minus ( ve) pressure Auto
Class II Minus ( ve) pressure

Class II

Class II
Class II Minus ( ve) pressure

Minus

(

ve)

pressure

Class II Minus ( ve) pressure
Class II Minus ( ve) pressure
Class II Minus ( ve) pressure
Class II Minus ( ve) pressure
Class II Minus ( ve) pressure
Class II Minus ( ve) pressure
Class II Minus ( ve) pressure
Class II Minus ( ve) pressure
Class II Minus ( ve) pressure
Class II Minus ( ve) pressure
Entry Change room ( ve) Class II Minus ( ve) pressure Auto

Auto

C U Rajasthan- Health & Environmental Safety

66

CENTRAL UNIVERSITY OF RAJASTHAN Bio Safety Level IV • Hazard group 4, P1 -P4 •

CENTRAL UNIVERSITY OF RAJASTHAN

Bio Safety Level IV

Hazard group 4, P1 -P4

Keep rooms at negative pressure

Use Class III safety cabinet

Install airlock and shower

Use Class III safety cabinet • Install airlock and shower Optional air inlet Minus pressure -ve
Use Class III safety cabinet • Install airlock and shower Optional air inlet Minus pressure -ve

Optional

air inlet

cabinet • Install airlock and shower Optional air inlet Minus pressure -ve Class III Restricted entry
cabinet • Install airlock and shower Optional air inlet Minus pressure -ve Class III Restricted entry
cabinet • Install airlock and shower Optional air inlet Minus pressure -ve Class III Restricted entry
Minus pressure
Minus
pressure
Minus pressure -ve Class III

-ve

Class III
Class III

Class III

Class III
Class III
Class III

Restricted

entry

-ve

-ve

-ve
Shower -ve

Shower

-ve

Change room

Change room -ve

-ve

Change room -ve
Change room -ve
Change room -ve
Change room -ve
and shower Optional air inlet Minus pressure -ve Class III Restricted entry -ve Shower -ve Change
and shower Optional air inlet Minus pressure -ve Class III Restricted entry -ve Shower -ve Change

C U Rajasthan- Health & Environmental Safety

67

CENTRAL UNIVERSITY OF RAJASTHAN Laminar Air Flow (LAF) • Unidirectional straight uninterrupted airflow • Sufficient

CENTRAL UNIVERSITY OF RAJASTHAN

Laminar Air Flow (LAF)

CENTRAL UNIVERSITY OF RAJASTHAN Laminar Air Flow (LAF) • Unidirectional straight uninterrupted airflow • Sufficient

Unidirectional straight uninterrupted airflow

Sufficient

for

working

with

BSL1

agents

Generally used where protection is required for product/ work only

No protection to operator

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68

CENTRAL UNIVERSITY OF RAJASTHAN Design and working principles of Bio Safety Cabinets Bio Safety Cabinet

CENTRAL UNIVERSITY OF RAJASTHAN

Design and working principles of Bio Safety Cabinets

Design and working principles of Bio Safety Cabinets Bio Safety Cabinet I Bio Safety Cabinet Class

Bio Safety Cabinet I

Bio Safety Cabinet Class I

1.

Unidirectional air flow from working area towards HEPA filter aided exhaust

2.

Some protection to user &

environment

3.

No protection to worked

C U Rajasthan- Health & Environmental Safety

69

CENTRAL UNIVERSITY OF RAJASTHAN Design and working principles of Bio Safety Cabinets Bio Safety Cabinet

CENTRAL UNIVERSITY OF RAJASTHAN

Design and working principles of Bio Safety Cabinets

Design and working principles of Bio Safety Cabinets Bio Safety Cabinet II Bio Safety Cabinet Class

Bio Safety Cabinet II

Bio Safety Cabinet Class II

1.

Bi-directional flow of HEPA filter purified air

2.

Good protection to user, the

environment and the work

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70

CENTRAL UNIVERSITY OF RAJASTHAN Design and working principles of Bio Safety Cabinets Fan Inlet Anti

CENTRAL UNIVERSITY OF RAJASTHAN

Design and working principles of Bio Safety Cabinets

Fan
Fan

Inlet

and working principles of Bio Safety Cabinets Fan Inlet Anti blow-back valve + alarm Exhaust HEPA

Anti blow-back

valve + alarm

Exhaust

HEPA

Pre-filter

HEPA

C U Rajasthan- Health & Safety

Bio Safety Cabinet Class III

1. Generally used for the highest hazard category

for

2. Fully

sealed

shell

operator protection

3. Work is done by using gloved sleeved ports

4. Air is drawn into and out of the cabinet via ‘HEPA’ filters

Environmental

71

CENTRAL UNIVERSITY OF RAJASTHAN Biological Safety Cabinet Operation Procedure Clean Area Working Area Contaminated Area

CENTRAL UNIVERSITY OF RAJASTHAN

Biological Safety Cabinet Operation Procedure

OF RAJASTHAN Biological Safety Cabinet Operation Procedure Clean Area Working Area Contaminated Area C U Rajasthan-

Clean Area

Working Area

Contaminated Area

C U Rajasthan- Health & Environmental Safety

72

CENTRAL UNIVERSITY OF RAJASTHAN Never block the rear vents of the Bio-safety Cabinets It defeats

CENTRAL UNIVERSITY OF RAJASTHAN

Never block the rear vents of the Bio-safety Cabinets

Never block the rear vents of the Bio-safety Cabinets It defeats the basic principle of the

It defeats the basic principle of the BCSs.

C U Rajasthan- Health & Environmental Safety

73

CENTRAL UNIVERSITY OF RAJASTHAN Store required supplies outside of Bio-safety Cabinets C U Rajasthan- Health

CENTRAL UNIVERSITY OF RAJASTHAN

Store required supplies outside of Bio-safety Cabinets

OF RAJASTHAN Store required supplies outside of Bio-safety Cabinets C U Rajasthan- Health & Environmental Safety

C U Rajasthan- Health & Environmental Safety

74

CENTRAL UNIVERSITY OF RAJASTHAN Never use LAF/ BSCs as a substitute to fume hoods C

CENTRAL UNIVERSITY OF RAJASTHAN

Never use LAF/ BSCs as a substitute to fume hoods

OF RAJASTHAN Never use LAF/ BSCs as a substitute to fume hoods C U Rajasthan- Health

C U Rajasthan- Health & Environmental Safety

75

CENTRAL UNIVERSITY OF RAJASTHAN Keeping proper information/ record of the BSL Laboratory BSL-1 Laboratory BIOHAZARD

CENTRAL UNIVERSITY OF RAJASTHAN

Keeping proper information/ record of the BSL Laboratory

BSL-1 Laboratory

BSL-1 Laboratory BIOHAZARD All Personal Protective Equipment shall be removed prior to leaving this work area.
BSL-1 Laboratory BIOHAZARD All Personal Protective Equipment shall be removed prior to leaving this work area.

BIOHAZARD

All Personal Protective Equipment shall be removed prior to leaving this work area.

Eating, drinking, smoking applying cosmetics or lip balm and handling contacts lenses area

prohibited in this work area.

Name of infectious agent(s):

Transmission route(s):

Special requirements for entering this area:

Emergency contacts

Name:

Title:

Phone:

EH&S office

(M-F 8-5) 646-3327 (after hours) 911.

*Biosafety Level 2 is similar to BSL - 1 and is suitable for work involving agents of moderate potential

hazard to personnel and the environment. It differs from BSL -1 in that

1.Lab personnel have specific training in handling pathogenic agents and directed to competent scientist;

2. Access to the lab is limited when work is being conducted:

3. Extreme precautions are taken with contaminated sharp items and

4. Certain procedures in which infectious aerosols or splashes may be created are conducted in biological

safety cabinets or other physical containment equipment

New Mexico State

University

Environmental Health & Safety

Date

Information sheet must contain

Name of infectious agent(s)

Mode of transmission(s)

Requirements of entering lab

Emergency Contact Person, Phone

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76

CENTRAL UNIVERSITY OF RAJASTHAN Used injection and sharps are the other common source of bio-hazard

CENTRAL UNIVERSITY OF RAJASTHAN

Used injection and sharps are the other common source of bio-hazard contamination

and sharps are the other common source of bio-hazard contamination C U Rajasthan- Health & Environmental

C U Rajasthan- Health & Environmental Safety

77

CENTRAL UNIVERSITY OF RAJASTHAN • Scissors, scalpels, razor blades, pins, syringe needles and knives are

CENTRAL UNIVERSITY OF RAJASTHAN

CENTRAL UNIVERSITY OF RAJASTHAN • Scissors, scalpels, razor blades, pins, syringe needles and knives are sharp

Scissors, scalpels, razor blades, pins, syringe needles and knives are sharp so use care.

Always direct sharp edges and points away from yourself and others.

If you are cut or bleeding, let your teacher know immediately.

Use only as directed.

C U Rajasthan- Health & Environmental Safety

78

CENTRAL UNIVERSITY OF RAJASTHAN Safe handling of needles and sharps • Needles must not be

CENTRAL UNIVERSITY OF RAJASTHAN

Safe handling of needles and sharps

UNIVERSITY OF RAJASTHAN Safe handling of needles and sharps • Needles must not be recapped, bent,
UNIVERSITY OF RAJASTHAN Safe handling of needles and sharps • Needles must not be recapped, bent,

Needles must not be recapped, bent, sheared or removed from a disposable syringe. Used needles should be destroyed.

All used sharps must be placed in a rigid, hard-plastic, puncture- resistant container for disposal.

C U Rajasthan- Health & Environmental Safety

79

CENTRAL UNIVERSITY OF RAJASTHAN Safe handling of broken glass-ware Broken glassware within laboratory should not

CENTRAL UNIVERSITY OF RAJASTHAN

Safe handling of broken glass-ware

UNIVERSITY OF RAJASTHAN Safe handling of broken glass-ware Broken glassware within laboratory should not be handled
UNIVERSITY OF RAJASTHAN Safe handling of broken glass-ware Broken glassware within laboratory should not be handled

Broken glassware within laboratory should not be handled bare hands

with

Broken glassware should be disposed properly

C U Rajasthan- Health & Environmental Safety

80

CENTRAL UNIVERSITY OF RAJASTHAN Safety Supplies and Equipments C U Rajasthan- Health & Environmental Safety

CENTRAL UNIVERSITY OF RAJASTHAN

Safety Supplies and Equipments

C U Rajasthan- Health & Environmental Safety

81

CENTRAL UNIVERSITY OF RAJASTHAN Common Laboratory Safety Equipment Lab shower Eye wash station Regular Safety

CENTRAL UNIVERSITY OF RAJASTHAN

Common Laboratory Safety Equipment

UNIVERSITY OF RAJASTHAN Common Laboratory Safety Equipment Lab shower Eye wash station Regular Safety checks Fire

Lab shower Eye wash station

Laboratory Safety Equipment Lab shower Eye wash station Regular Safety checks Fire safety Personal protective

Regular Safety checks

Equipment Lab shower Eye wash station Regular Safety checks Fire safety Personal protective equipment Waste disposal
Equipment Lab shower Eye wash station Regular Safety checks Fire safety Personal protective equipment Waste disposal

Fire safety

shower Eye wash station Regular Safety checks Fire safety Personal protective equipment Waste disposal C U

Personal

protective

equipment

Safety checks Fire safety Personal protective equipment Waste disposal C U Rajasthan- Health & Environmental

Waste disposal

C U Rajasthan- Health & Environmental Safety

82

CENTRAL UNIVERSITY OF RAJASTHAN Personal protective equipment (PPE) Includes any devices or clothing worn by

CENTRAL UNIVERSITY OF RAJASTHAN

Personal protective equipment (PPE)

Includes any devices or clothing worn by the worker to

protect against the hazards in the environment.

Examples

Safety glasses

Safety masks

Lab coats & Shoes

Protective gloves,

C U Rajasthan- Health & Environmental Safety

83

CENTRAL UNIVERSITY OF RAJASTHAN Protective Laboratory Goggles/ Masks • Put on your safety glasses and

CENTRAL UNIVERSITY OF RAJASTHAN

Protective Laboratory Goggles/ Masks

UNIVERSITY OF RAJASTHAN Protective Laboratory Goggles/ Masks • Put on your safety glasses and mask before
UNIVERSITY OF RAJASTHAN Protective Laboratory Goggles/ Masks • Put on your safety glasses and mask before

Put on your safety glasses and mask before entering the lab.

You never know what may come flying off of a work bench, a fume hood, or a refrigerator

Use of protective masks minimizes the chances for inhalation dependent exposure

C U Rajasthan- Health & Environmental Safety

84

CENTRAL UNIVERSITY OF RAJASTHAN Protective Clothing (Lab Coat)  Must covers arms and legs 

CENTRAL UNIVERSITY OF RAJASTHAN

Protective Clothing (Lab Coat)

UNIVERSITY OF RAJASTHAN Protective Clothing (Lab Coat)  Must covers arms and legs  Lab coats

Must covers arms and legs

Lab coats with closed fasteners

Non-flammable, non-porous aprons when using corrosives

Remove before leaving the lab

Launder separately

C U Rajasthan- Health & Environmental Safety

85

CENTRAL UNIVERSITY OF RAJASTHAN Lab coat, goggles and glove practice Inappropriate Appropriate C U Rajasthan-

CENTRAL UNIVERSITY OF RAJASTHAN

Lab coat, goggles and glove practice

UNIVERSITY OF RAJASTHAN Lab coat, goggles and glove practice Inappropriate Appropriate C U Rajasthan- Health &

Inappropriate

RAJASTHAN Lab coat, goggles and glove practice Inappropriate Appropriate C U Rajasthan- Health & Environmental Safety

Appropriate

C U Rajasthan- Health & Environmental Safety

86

CENTRAL UNIVERSITY OF RAJASTHAN Personal protection before entering the lab • Minimize exposed skin at

CENTRAL UNIVERSITY OF RAJASTHAN

Personal protection before entering the lab

Minimize exposed skin at all times by wearing the appropriate clothing

Use appropriate protective equipment at all times in the lab when chemical, radioactive, or bio-hazardous materials are

being handled.

C U Rajasthan- Health & Environmental Safety

87

CENTRAL UNIVERSITY OF RAJASTHAN Emergency Laboratory Shower 1. Each lab should be equipped with an

CENTRAL UNIVERSITY OF RAJASTHAN

Emergency Laboratory Shower

CENTRAL UNIVERSITY OF RAJASTHAN Emergency Laboratory Shower 1. Each lab should be equipped with an emergency
CENTRAL UNIVERSITY OF RAJASTHAN Emergency Laboratory Shower 1. Each lab should be equipped with an emergency

1. Each lab should be equipped with an emergency shower at an un- obstructed and easily accessible location within the laboratory

preferably within 10 seconds

2. Laboratory showers pour water at ~ 20 gals. / min. (compare to 2 gals. / min.) of standard bathroom shower

C U Rajasthan- Health & Environmental Safety

88

CENTRAL UNIVERSITY OF RAJASTHAN Eye Wash Station Each lab should be equipped with an eye

CENTRAL UNIVERSITY OF RAJASTHAN

Eye Wash Station

CENTRAL UNIVERSITY OF RAJASTHAN Eye Wash Station Each lab should be equipped with an eye wash
CENTRAL UNIVERSITY OF RAJASTHAN Eye Wash Station Each lab should be equipped with an eye wash

Each lab should be equipped with an eye wash station

C U Rajasthan- Health & Environmental Safety

89

CENTRAL UNIVERSITY OF RAJASTHAN How to use an Eye Wash Station • Shout for help

CENTRAL UNIVERSITY OF RAJASTHAN

How to use an Eye Wash Station

UNIVERSITY OF RAJASTHAN How to use an Eye Wash Station • Shout for help • Hold

Shout for help

Hold your eyelids open

with your fingers as you

walk towards eye wash unit

Rinse your eyes thoroughly

Ask a co-worker to watch clock to make sure that you continued to rinse your eyes for at least 15 minutes

Ask a co-worker to collect

MSDS and visit a doctor

Regular testing and maintenance of Emergency Shower, Eye Wash Station & document test reports

C U Rajasthan- Health & Environmental Safety

90

CENTRAL UNIVERSITY OF RAJASTHAN Personal protective equipments Many types of 1. Chemical Fume Hoods 2.

CENTRAL UNIVERSITY OF RAJASTHAN Personal protective equipments

Many types of

1. Chemical Fume Hoods

2. Laminar Air Flow

3. Bio-safety Cabinets

of 1. Chemical Fume Hoods 2. Laminar Air Flow 3. Bio-safety Cabinets C U Rajasthan- Health
of 1. Chemical Fume Hoods 2. Laminar Air Flow 3. Bio-safety Cabinets C U Rajasthan- Health

C U Rajasthan- Health & Environmental Safety

91
91
CENTRAL UNIVERSITY OF RAJASTHAN Other Safety Equipments 1. Fire extinguishers are important means of laboratory

CENTRAL UNIVERSITY OF RAJASTHAN Other Safety Equipments

CENTRAL UNIVERSITY OF RAJASTHAN Other Safety Equipments 1. Fire extinguishers are important means of laboratory

1.

Fire extinguishers are important means of laboratory safety.

2.

Should be used as per recommendations and only after appropriate training.

3.

Never use fire extinguisher for large fires

C U Rajasthan- Health & Environmental Safety

92

CENTRAL UNIVERSITY OF RAJASTHAN Other Safety Equipments 1. First Aid Kit should be accessible to

CENTRAL UNIVERSITY OF RAJASTHAN Other Safety Equipments

CENTRAL UNIVERSITY OF RAJASTHAN Other Safety Equipments 1. First Aid Kit should be accessible to all

1. First Aid Kit should be accessible to all workers in the lab

2. First Aid Kits should be stocked and maintained regularly

3. Laboratory workers should be trained for use of First Aid Kit

C U Rajasthan- Health & Environmental Safety

93

CENTRAL UNIVERSITY OF RAJASTHAN Safe Laboratory Practices C U Rajasthan- Health & Environmental Safety 94

CENTRAL UNIVERSITY OF RAJASTHAN

Safe Laboratory Practices

C U Rajasthan- Health & Environmental Safety

94

CENTRAL UNIVERSITY OF RAJASTHAN 5. Follow rules & guidelines Dos 95 C U Rajasthan- Health

CENTRAL UNIVERSITY OF RAJASTHAN

5. Follow rules & guidelines

Dos

CENTRAL UNIVERSITY OF RAJASTHAN 5. Follow rules & guidelines Dos 95 C U Rajasthan- Health &
CENTRAL UNIVERSITY OF RAJASTHAN 5. Follow rules & guidelines Dos 95 C U Rajasthan- Health &
CENTRAL UNIVERSITY OF RAJASTHAN 5. Follow rules & guidelines Dos 95 C U Rajasthan- Health &
CENTRAL UNIVERSITY OF RAJASTHAN 5. Follow rules & guidelines Dos 95 C U Rajasthan- Health &

95

CENTRAL UNIVERSITY OF RAJASTHAN 5. Follow rules & guidelines Dos 95 C U Rajasthan- Health &
CENTRAL UNIVERSITY OF RAJASTHAN 5. Follow rules & guidelines Dos 95 C U Rajasthan- Health &

C U Rajasthan- Health & Environmental Safety

CENTRAL UNIVERSITY OF RAJASTHAN Don’ts C U Rajasthan- Health & Environmental Safety 96

CENTRAL UNIVERSITY OF RAJASTHAN

CENTRAL UNIVERSITY OF RAJASTHAN Don’ts C U Rajasthan- Health & Environmental Safety 96

Don’ts

CENTRAL UNIVERSITY OF RAJASTHAN Don’ts C U Rajasthan- Health & Environmental Safety 96
CENTRAL UNIVERSITY OF RAJASTHAN Don’ts C U Rajasthan- Health & Environmental Safety 96

C U Rajasthan- Health & Environmental Safety

96

CENTRAL UNIVERSITY OF RAJASTHAN Floor- plans with fire exit procedure C U Rajasthan- Health &

CENTRAL UNIVERSITY OF RAJASTHAN

Floor- plans with fire exit procedure

CENTRAL UNIVERSITY OF RAJASTHAN Floor- plans with fire exit procedure C U Rajasthan- Health & Environmental
CENTRAL UNIVERSITY OF RAJASTHAN Floor- plans with fire exit procedure C U Rajasthan- Health & Environmental

C U Rajasthan- Health & Environmental Safety

97

CENTRAL UNIVERSITY OF RAJASTHAN Safety from fire C U Rajasthan- Health & Environmental Safety 98

CENTRAL UNIVERSITY OF RAJASTHAN

Safety from fire

C U Rajasthan- Health & Environmental Safety

98

CENTRAL UNIVERSITY OF RAJASTHAN Fire: The other very common lab hazard Fire Safety • All

CENTRAL UNIVERSITY OF RAJASTHAN

Fire: The other very common lab hazard

Fire Safety

All employees are responsible for safety.

Know the location of fire extinguishers .

Learn how to use them correctly.

Know where the fire blanket is.

Know the procedure for reporting a fire.

Institution/ University should organize fire safety training/ drills.

C U Rajasthan- Health & Environmental Safety

CENTRAL UNIVERSITY OF RAJASTHAN Fire Safety Components of a fire • Fuel • Oxygen •

CENTRAL UNIVERSITY OF RAJASTHAN

Fire Safety

Components of a fire

Fuel

Oxygen

Heat

Necessary chain reaction

C U Rajasthan- Health & Environmental Safety

CENTRAL UNIVERSITY OF RAJASTHAN Fire Safety Classification of fires 1. Class A fires Occur with

CENTRAL UNIVERSITY OF RAJASTHAN

Fire Safety

Classification of fires

1.

Class A fires

Occur with ordinary combustible material, such as wood, rubber, paper, cloth, and many forms of plastics.

2.

Class B fires

Occur in a vaporair mixture over flammable solvents, such as gasoline, oil, paint, lacquers, grease, and flammable gases.

C U Rajasthan- Health & Environmental Safety

CENTRAL UNIVERSITY OF RAJASTHAN Fire Safety Classification of fires 3. Class C fires Occur in

CENTRAL UNIVERSITY OF RAJASTHAN

Fire Safety

Classification of fires

3.

Class C fires

Occur in or near electrical equipment.

4.

Class D fires

Occur with combustible metals, such as magnesium, sodium, and lithium.

(Class D fires should be fought by firefighters only)

Courtesy of Health and Environmental Safety, The University of Texas Health Sciences Center at Houston

Safety, The University of Texas Health Sciences Center at Houston CENTRAL UNIVERSITY OF RAJASTHAN Fire Safety

CENTRAL UNIVERSITY OF RAJASTHAN

Fire Safety

Safety, The University of Texas Health Sciences Center at Houston CENTRAL UNIVERSITY OF RAJASTHAN Fire Safety
CENTRAL UNIVERSITY OF RAJASTHAN Fire Safety Fire Extinguishers 1. Type A extinguishers • Contain soda

CENTRAL UNIVERSITY OF RAJASTHAN

Fire Safety

Fire Extinguishers

1.

Type A extinguishers

Contain soda and acid or water and are used to cool the ordinary fire such as wood, cloth or paper.

2.

Type BC extinguishers

Contain foam, dry chemicals, or carbon dioxide (CO2).

Are used to combat fires occurring in vaporair mixtures

over solvents such as grease, gasoline or oil fires.

CENTRAL UNIVERSITY OF RAJASTHAN Fire Extinguishers 3. Type ABC extinguishers • Contain a dry chemical

CENTRAL UNIVERSITY OF RAJASTHAN

Fire Extinguishers

3. Type ABC extinguishers

Contain a dry chemical and are used on fires of wood, cloth, paper, oil, grease, and gasoline.

Multipurpose in combating fires and thus, these are

best suited for use in research laboratories.

C U Rajasthan- Health & Environmental Safety

CENTRAL UNIVERSITY OF RAJASTHAN Fire fighting with Fire extinguishers P. A. S. S P =

CENTRAL UNIVERSITY OF RAJASTHAN

Fire fighting with Fire extinguishers

P. A. S. S

RAJASTHAN Fire fighting with Fire extinguishers P. A. S. S P = pull pin A =

P = pull pin A = aim at fire S = squeeze lever

S = swipe side to side

CENTRAL UNIVERSITY OF RAJASTHAN Heat Hazard in the laboratory • Use an oven mitt or

CENTRAL UNIVERSITY OF RAJASTHAN

Heat Hazard in the laboratory

Use an oven mitt or gloves or clamp when handling hot materials.

Always heat objects in a test tube holding away from you and others.

Hot plates, hot water and hot glassware can cause burns.

Glassware looks the same when hot or cold, so use care when heating any type of glass.

Rapid changes in glass temperature can cause it to expand, crack and/or explode!

Never touch a hot plate, oven trays etc. with bare hands.

temperature can cause it to expand, crack and/or explode! • Never touch a hot plate, oven
CENTRAL UNIVERSITY OF RAJASTHAN Emergency Response to Possible Fire • Things to do in a

CENTRAL UNIVERSITY OF RAJASTHAN

Emergency Response to Possible Fire

Things to do in a fire response

1. Pull the nearest fire alarm

2. Close windows and doors (minimize O 2 influx)

3. Use an ABC type extinguisher for small fire

4. If fire is too big, leave the area immediately by stairs

5. Drop to ground and roll or crawl to exit

C U Rajasthan- Health & Environmental Safety

CENTRAL UNIVERSITY OF RAJASTHAN Your contribution towards making labs safe Phlebotomy Handbook: Blood Collection

CENTRAL UNIVERSITY OF RAJASTHAN

Your contribution towards making labs safe

Phlebotomy Handbook: Blood Collection Essentials, Seventh Edition Diana Garza • Kathleen Becan-McBride

CENTRAL UNIVERSITY OF RAJASTHAN Students and researchers: • Complete Laboratory Safety Training • Ensure complete

CENTRAL UNIVERSITY OF RAJASTHAN

Students and researchers:

Complete Laboratory Safety Training

Ensure complete compliance with the laboratory safety training

Ensure own safety and of others

the laboratory safety training • Ensure own safety and of others C U Rajasthan- Health &

C U Rajasthan- Health & Environmental Safety

110

CENTRAL UNIVERSITY OF RAJASTHAN How do we ensure safety for all C U Raj. family

CENTRAL UNIVERSITY OF RAJASTHAN

How do we ensure safety for all C U Raj. family members ?

University Safety Training Programme

Teach – What! (PPT modules and Reading Materials) Train- How! (Hands-on Training & Demos) Test
Teach – What!
(PPT modules
and Reading
Materials)
Train- How!
(Hands-on
Training &
Demos)
Test
(Online safety Quiz)
CENTRAL UNIVERSITY OF RAJASTHAN

CENTRAL UNIVERSITY OF RAJASTHAN

CENTRAL UNIVERSITY OF RAJASTHAN