PATENT ABSTRACTS

801

5610040
ENZYMATIC SYNTHESIS OF CERAMIDES AND HYBRID CERAMIDES Smeets Jan W;De Pater Robertus M; Lambers Johannes W J Viaardingen, NETHERLANDS Assigned to Gist-brocades n v PCT No. PCT/EP94/01495 Sec. 371 Date May 17, 1995 Sec. 102(e) Date May 17, 1995 PCT Filed May 6, 1994 PCT Pub. No. WO94/26919 PCT Pub. Date Nov. 24, 1994. The present invention provides an efficient method for the production of ceramides and hybrid ceramides via the selective N-acylation of glycosphingolipids by reacting an organic acid or ester thereof with a lysosphingolipid in the presence of a lipase in an organic medium.

superimposed on the heat transfer chamber with the heat conductive floor being disposed in separating relation therebetween so as to permit conductive and convective heating of the primary chamber, thus causing heating of the waste in the primary chamber. An exhaust vent in fluid communication with the heat transfer chamber permits venting the fumes to the ambient surroundings.

5612198 5611289
GASIFIER FOR BIOMASS WASTE AND RELATED VOLATILE SOLIDS PRODUCTION INSULIN-LIKE FACTOR-1 METHYLOTROPHIC CELLS Brookes Davi Mississanga, Ontario, CANADA A gasifier is disclosed. The gasifier comprises a primary chamber for receiving therein biomass waste material and other related volatile solids to be gasified. A fume transfer vent permits the escape of fumes from the primary chamber. A mixing chamber accepts the fumes from the fume transfer vent. The fumes then flow to an afterburner chamber where a burner member produces a heating flame so as to cause the additional full oxidization of the constituents of the fumes so as to oxidize the constituents. A partitioning wall is disposed between the flame chamber and the primary chamber so as to preclude the heating flame from entering the primary chamber and to also preclude the radiation from the heating flame from directly entering the primary chamber, thereby precluding direct contact and physical disturbance of the waste material. A heat transfer chamber in fluid communication with the afterburner chamber accepts the fully oxidized fumes therefrom. The heat from the full oxidation of the fumes causes heating of the heat transfer chamber. The primary chamber has a heat conductive floor and is Brierley Russell A; Davis Geneva; Holtz Gregory C; Gleeson Martin; Howard Bradley Exton, PA, UNITED STATES Assigned to The Salk Institute Insulin-like growth factor-I (IGF-I), a naturally occurring, relatively short, single chain polypeptide, is prepared by growing methylotrophic yeast transformants containing in their genome at least one copy of DNA encoding IGF- 1, in operational linkage with DNA encoding a signal sequence, which is effective for directing secretion of proteins from the host cells and which also includes the proteolytic processing site lys-arg and may include one or more glu-ala sequences. In preferred embodiments the signal sequence is the S. cerevisiae alpha mating factor pre-pro sequence. Expression of both the DNA encoding IGF-1 and the pre-pro signal sequence is regulated by a promoter region derived from a methanol responsive gene of a methylotrophic yeast. DNA constructs and recombinant methylotrophic yeast strains used for the expression and secretion of IGF-I are also provided. For preferred embodiments, protease deficient Pichia pastoris strains are provided. OF GROWTH IN YEAST