Upright Microscope ECLIPSE Ni

Upright Microscope
System expandability
Nikons proprietary stratum structure enables effcient system construction.
The numerous accessories can be custom combined depending upon application.
Optical performance
CFI Plan Apochromat objectives with chromatic aberration correction and high
transmission throughout a broad range of wavelengths
Objectives with improved transmission in near IR wavelength range for multiphoton
excitation imaging
Design
3D ergo design combines functionality with sophistication.
Operability
Ni-E: Motorized model with automatic changeover of observation methods and
adjustment of microscope accessories.
Ni-U: Manual model with some motorized options.
Most microscope controls can be operated with easy-to-reach buttons on the front of
the Ni-E.
The Ni series, the ultimate in upright biological microscope evolution, supports bioscience studies with
enhanced basic performance and fexible system expandability.
The newly developed CFI Plan Apochromat (lambda) series objectives are the key to the series
optical performance. Nano Crystal Coat, with its ultra low refractive index, is employed for the frst time
in microscope objectives, providing brighter, high-resolution and high-contrast microscopy images.
Nikons proprietary stratum structure, used and highly acclaimed for inverted research microscopes,
is now incorporated in an upright microscope, allowing a combination of components to be installed.
Applications using laser and fuorescent proteins, such as Kaede and PA-GFP, are possible with the
addition of a two-tiered fuorescent unit and a photoactivation unit.
The Ni series transcends the concept of conventional upright microscopes and expands the possibilities
of advanced research in felds such as bioscience and medicine.
2 3
See the evolution
4 5
The Ni series microscopes meet all research demands
These microscopes have three features: expandability of the system due to the stratum structure
that enables optical paths in two tiers; superior optical performance, as represented by the Plan
Apochromat series objectives; and high-speed motorized accessories. The Ni series is designed to
meet the needs of all advanced bioscience and medical research.
Cell biology
Pathology
Neuroscience
Developmental
biology
Immunology
Genetics
Microbiology
Drug discovery
Cancer research
Microscopy images courtesy of:
(Left) Torsten Whitmann
(Center) Michael W. Davidson
(Right) Dr. Jennifer Waters and Adrian Salic
Ni-E
6 7
Expandable system broadens application possibilities
Photo-activation imaging (Ni-E/Ni-U)
Research into the reactions and changes of stimulated cells has become popular in recent years. Nikon has developed a
new photoactivation unit for upright microscopes, a frst in microscopy.
Photo
activation
FRAP
Ni-E Ni-U
Flexible stratum structure
This structure allows two-layer mounting of a photoactivation unit with an epi-fuorescence attachment.
Objectives for long-wavelength laser
With CFI Plan Apochromat objectives, chromatic aberration has been corrected up to 850 nm and
transmission improved in the long wavelength range, increasing accuracy and effciency of laser excitation
at target site.
Multi-color uorescent imaging (Ni-E)
Demand for multi-color fuorescent imaging that uses newly developed fuorescent proteins and fuorescent reagents is
constantly increasing. Nikon meets such needs with diverse functions and optical technologies.
Multi-color
uorescent
observation
FISH
Signal
conversion
High-speed motorized components
The higher speeds of the motorized excitation and barrier flter wheel and the motorized shutter enable
quick wavelength changes, reducing photobleaching of the specimen.
It can be operated via easily accessible control buttons, increasing operation effciency.
CFI Plan Apochromat series objectives
Transmission and chromatic aberration correction have been improved throughout the wide range of visible
to near IR wavelengths, allowing use of various fuorescent reagents. They provide bright, high-contrast,
high S/N (signal-to-noise) ratio multi-color fuorescence images with almost no focus shift when used with
any wavelength.
Mounted motorized excitation/barrier
flter wheel
Mounted photoactivation unit

0
Complies with FDA performance standard for laser products except for deviations pursuant to Laser Notice No.50,dated June 24,2007.
IEC/EN60825-1:2007 Total Power 500mWMAX. CW400700nm
Ni-E
8 9
Multiphoton imaging (Ni-E)
Multiphoton microscopy in which long excitation wavelengths are used to allow less-invasive imaging of ever deeper areas of
cells is gaining popularity. The design of the Ni-E model is optimized for multiphoton imaging in both optical and mechanical
systems to meet the demands of today.
In vivo
imaging
Two-photon
imaging
Photo
activation
Objective dedicated to multiphoton imaging
CFI75 Apochromat 25xW MP objective features chromatic aberration correction over a broad wavelength
range from 405 to 950 nm, high NA (numerical aperture) (1.10) and longer working distance (2.0 mm), and
compatibility with water immersion and water dipping.
High-sensitivity multiphoton detector (NDD)
Newly developed episcopic NDD (non-descanned detector) unit incorporates a detector that effciently
senses weak signals from deep areas of live specimens. In combination with a diascopic NDD unit,
transmitted signals can be also detected.
Retrofittable focusing nosepiece mechanism
Microscopes can be modifed by switching the focusing stage and focusing nosepiece, enabling fxed-stage
confguration to meet demands of experiments such as in vivo imaging.
Confguration of manual microscope Ni-U with
motorized epi-fuorescence cube turret, epi-
fuorescence attachment, motorized septuple
nosepiece, digital camera DS-Qi1 and DS-Fi2, control
unit DS-L3, simple remote control pad, control box B
Confguration of motorized microscope Ni-E with
motorized quadrocular tilting tube, motorized
DSC zooming port, motorized ND flter, digital
camera DS-Fi2, motorized XY stage and joystick,
control unit DS-L3, control box A
Enhanced system expandability provides
the best solution
Ni series accessories are segmented by function, allowing you to select required units and
fexibly combine them to create lean and effective system confgurations.
System conguration for acquiring multi-stained specimen images
The motorized epi-fuorescence cube turret shutter, which helps to reduce photobleaching of specimens, is easily operated with
a convenient remote control pad.
Images are automatically acquired by controlling the motorized accessories, including the epi-fuorescence cube turret
according to the camera settings such as exposure time, camera gain, and time interval.
This confguration is recommended for those who conduct mostly fuorescent observations rather than brightfeld observations.
System conguration for acquiring pathological specimen images
Optimal brightness can be automatically adjusted with objective changeover, eliminating the need for manual adjustment.
As operations for optical zooming can be controlled from the control unit DS-L3, images of the desired angle of feld can
be captured while maintaining image quality.
Mounted episcopic NDD unit
and diascopic NDD unit

10 11
Versatile microscopes meet all demands
Ni-U (manual model)
Ergonomic tube and stage height adjustment mechanism allow
comfortable viewing positions.
Stratum structure and sturdy design improve expandability.
Motorized nosepiece, motorized epi-fluorescence cube turret,
motorized shutter can be utilized.
Ni-E (motorized model)
High-precision motorized focusing
Broad range of motorized accessories that can be used in combination.
Observation methods can be changed at a simple push of a button.
Stratum structure and sturdy design improve expandability.
3D ergo design buttons with improved operability are located close together for speedy operations.
Microscope settings in use can be verified on the display.
Optimized for multiphoton excitation imaging
Two focusing mechanism options: focusing stage and focusing nosepiece
Manual and motorized models
To meet diverse user demands for operability, system expandability and motorized control, Nikon provides two Ni series
models. The Ni-U, which has compatibility with some motorized accessories, is the manual model suitable for high-quality
image observation and digital imaging. The Ni-E is a fully motorized model that is effcient for experiments requiring
comprehensive control of various devices, such as photoactivation units and confocal systems.
Ni-E provides fully motorized operations
Automatic adjustment with objective changeover
Condenser, aperture and feld diaphragm, and ND flter are automatically set to the optimal
position during objective changeover. In addition, stage XYZ travel amount per handle
rotation and parfocal distance deviation correction are automatically adjusted.
Microscope settings can also be manually adjusted.
Observation mode changeover
Observation methods such as brightfeld, DIC, phase contrast and fuorescence can be
designated individual buttons, enabling changes to be made at the push of a button. This is
particularly useful when the same sample is imaged with multiple observation methods.
High-precision motorized focusing
High-precision Z-focus incorporated by the Ni-E provides accurate height information
required for use with confocal laser microscopes. Individual coarse and fne focus knobs
provide enhanced ease of operation.
Ni-E confgured with a motorized epi-fuorescence cube turret
and motorized universal condenser
Ni-U confgured with an ergonomic binocular tube
12 13
Lens Lens
Reflected light Reflected light Incident light Incident light
Nano Crystal Coat
This anti-refective coating that consists of nanometer-size particles is based on
semiconductor manufacturing technology and is also used for Nikon camera lenses.
The coarse structure with particles arranged in a spongy construction with uniform
spaces between them enables extremely low refractive indices.
Supreme optical performance
As a light microscope manufacturer, Nikon has cultivated high technical capabilities and confdence. With its advanced
technologies extending from optical glass production to lens design, fabrication, coating and processing, Nikon provides
unsurpassed optical performance.
CFI Plan Apochromat series
With remarkably high NA, greatly improved transmission in the long wavelength
range thanks to Nikon proprietary Nano Crystal Coat, and chromatic aberration
correction over 435 to 850 nm, these objectives are ideally suited not only for
brightfeld and DIC observations but also for fuorescent observations. These lenses
allow acquisition of bright and clear images at any wavelength for near-IR imaging
and multi-color fuorescence imaging. Because bright images can be captured even
with a weak excitation light, damage to a specimen is minimized.
Water dipping objective lenses
With a long working distance and high NA, these objectives provide excellent
transmission in near IR wavelength range. The axial chromatic aberration of 40x and
60x objectives has been corrected to up to 850 nm, allowing high-resolution images
of minute structures in thick specimens during IR-DIC observation.
The 25xW MP and 100x objectives feature high NA (1.1) and a long working distance
(2.0 mm). With chromatic aberration corrected in the IR region, these objectives are
ideal for multiphoton excitation observation. In addition, by employing a mechanism
to compensate for the changes in spherical aberration that occur at different
temperatures and depths of observation points, clear images of areas deep within a
thick specimen can be captured.
Uniformly bright illumination
The fy-eye lens is ideally suited to diascopic illumination optical systems.
Uniform and bright illumination up to the viewfeld periphery is provided at
any magnifcation.
High-performance objective lens
Epi-fuorescence observation
Phase contrast observation
DIC (Differential Interference Contrast) observation
Brightfeld observation
Excellent image acquisition with all observation methods
Fluorescence
filter cube
Lens
Camera
Light
source
Light absorbing
material
ND
Stray light
Technologies supporting the Ni series
Conventional multi-layer coating
Without Nano Crystal Coat With Nano Crystal Coat
Nano Crystal Coat
Fluorescence noise elimination
Nikons proprietary noise terminator mechanism is employed in the epi-
fuorescence cube turret and flter cubes. The S/N ratio has been dramatically
improved by thoroughly eliminating stray light in the flter cubes, allowing
images of weak fuorescent signals to be captured with high contrast and
brightness.
14 15
Digital imaging
The Ni series can be controlled in conjunction with a Digital Sight series digital camera, facilitating
effortless digital imaging. Images can be captured with a dedicated button on the microscope body,
the touch panel on the camera control unit, or the software GUI on a PC, depending on your preference.
Ultimate ease, speed and clarity in imaging
Digital Sight series digital cameras
A wide array of models with high sensitivity, high resolution, high speeds and faithful
color reproduction are available to meet all research needs and purposes.
Confocal imaging
The Ni series is sturdy enough for the incorporation of a confocal scanner.
The broad fux of light allows the capture of bright confocal images.
Image capture button
Images can be acquired by simply pressing the image capture button located on the
microscope base.
Multiphoton confocal microscope A1R MP
+
A1R MP
+
is capable of high-speed multiphoton imaging up to 420 fps. The
NDD enables deep specimen imaging with high sensitivity. High-speed,
accurate GFP/YFP unmixing is possible with NDD.
Confocal microscope A1R
+
/A1
+
A1
+
incorporates a high-defnition (up to 4096 x 4086 pixels) non-resonant
scanner. A1R
+
also incorporates a high-speed (up to 420 fps) resonant
scanner, enabling simultaneous photo activation and imaging.
Confocal microscope C2
+
C2
+
features a compact design and high operability, and it supports
4-channel simultaneous acquisition. It provides various imaging such as
image stitching (large images).
Spectral imaging confocal microscope
A1Rsi
+
/A1si
+
/C2si
+
By incorporating a spectral detector, a wide wavelength spectrum of
320 nm can be acquired with a single scan. Closely overlapping spectra can
be unmixed cleanly with no crosstalk.
High-sensitivity cooled monochrome camera head
DS-Qi1
The new CCD drive circuit reduces readout noise.
The cooling mechanism reduces heat-induced noise,
allowing the capture of weak fuorescence signals.
Furthermore, there is a high frame rate of up to
48 fps and a high quantitative linearity within 2%.
Ultrahigh-resolution cooled color camera head
DS-Ri1
Provides the 12.7-megapixel equivalent, 2200 TV line
high defnition. Smooth live image display at max.
32 fps makes focusing easy. Superior color
reproduction capabilities allow recording of accurate
specimen colors. The cooling mechanism reduces
heat-induced noise in fuorescent images.
High-denition cooled color camera head
DS-Fi1c
Equipped with a 5-magapixel CCD. The cooling
mechanism reduces heat-induced noise, making it
ideal for fuorescent image acquisition.
High-denition color camera head
DS-Fi2
Equipped with a 5-magapixel CCD. Displays live
images at max. 21 fps. Minute structures can be
captured in brightfeld, phase contrast and DIC
observations.
Integrated imaging software
Nikons imaging software NIS-Elements employs a single platform to allow stress-free
operations, from image capture through to comprehensive control of camera, microscope and
peripheral devices, to image processing, measurement, data management and analysis.
Stand-alone control unit DS-L3
Stand-alone control unit DS-L3 allows the Digital Sight series digital camera to be set
up and operated without a PC connection. It also enables image acquisition from a PC
through a network. The DS-L3 is frmware based, and therefore will not compromise your
facilitys network.
Optimal imaging parameters for each observation method can be selected
using icons and simple measurements can be taken.
Camera, motorized microscope functions and peripheral devices can be
comprehensively controlled.
Microscope status data, such as objective lens, fluorescent filter cube and
condenser information, is automatically saved along with the
captured image when a motorized or intelligent unit is attached.
Operations can be controlled with a mouse or a touch panel operated by
touch or stylus pen.
Required icons can be selected and laid out as desired.
Image capture button
Camera/microscope control Microscope control Scene mode icons
Ni-E confgured with C2
+
16 17
Wide array of accessories fnely segmented by function
Motorized changeover of optical paths is possible. Eyepiece
inclination can be adjusted from 15 to 35.
Effective for applications that require highly accurate
positioning, such as photo-activation imaging and FISH.
High-speed motorized changeover of condenser lenses
for brightfeld, phase contrast, DIC and simple darkfeld
observations is possible.
Laser light photo activation and episcopic illumination are
possible. Both the photoactivation unit and the confocal
system can be used with a single laser source.
Noise terminator provides high S/N ratio. Six flter cubes
can be installed. Either an epi-fuorescence attachment or
a photoactivation unit can be attached.
A digital camera can be mounted on the camera port. A
motorized 0.6x - 2.0x zoom optical system is incorporated.
Makes control of motorized XY stage possible.
Barrier flter positions (7 positionsusing 25 mm flters)
can be changed at a high speed of 0.2 sec. per position.
Objective magnifcation is automatically saved along with
the captured image.
High-speed shutter control is possible. The shutter can be
attached to diascopic and episcopic illumination systems.
Brightness is automatically optimized with the changeover
of the motorized nosepiece. Motorized adjustment of
desired brightness is also possible.
In addition to motorized microscope operation, XYZ control
of stage is possible with similar operational ease as that of
an actual microscope.
Excitation flter positions (8 positionsusing 25mm flters)
can be changed at a high speed of 0.15 sec. per position.
Objective magnifcation is automatically saved along with
the captured image. Built-in prism/analyzer plate slot.
Motorized operation of nosepiece, epi-fuorescence cube
turret and shutter is possible.
Motorized quadrocular tilting tube (Ni-E)
Motorized XY stage (Ni-E)
Motorized universal condenser Dry (Ni-E)
Photoactivation unit (Ni-E, Ni-U)
Motorized epi-uorescence cube turret (Ni-E,
Ni-U)
Motorized DSC zooming port (Ni-E)
Joystick for motorized stage (Ni-E)
Motorized barrier lter wheel (Ni-E)
Motorized septuple nosepiece (Ni-E, Ni-U)
Motorized shutter (Ni-E, Ni-U)
Motorized ND lter (Ni-E)
Ergo controller (Ni-E)
Motorized excitation lter wheel (Ni-E)
Motorized sextuple DIC nosepiece (Ni-E, Ni-U)
Simple remote control pad (Ni-U)
Elegant design with absolute comfort
Our design goal for the Ni series was to provide physical and mental comfort
during use. Straight lines and fat surfaces that are traditionally employed in
microscope design do not exist in nature, so they induce a feeling of tension
while seated in front of the microscope. The Ni series employs a modern
organic design in the microscope wing that adds a gentle beauty while
reducing stress during lengthy observations.
When designing the Ni series, Nikon collaborated with Akio Shindate,
previously involved in the design of Nikon SLR cameras from the early stage
of concept building. Through this collaboration with Shindate, who excels
at unique ideas and stylish designs, Nikon has produced innovative new
microscopes that combine function with beauty.
Design for function
The Ni series embodies the basic spirit of a product design for minimizing
user fatigue.
3D ergo design for comfortable touch-type operations
Buttons on microscope sides are angled to allow touch-type operations
during observation.
Easy to see display
Microscope settings are shown on a display that can be easily viewed
from the observation position.
Small footprint
Space-saving microscope base assures larger work space.
Contrast between elegance and sharpness
The white microscope wings feature an innovative and elegant curved
design. In contrast, the rectilinear and black microscope base reflects
Nikons image of high quality and high performance. This new design will
also be employed in all accessories and Ci series clinical and laboratory
microscopes, and will continue in future models.
Akio Shindate (Director, VO Corporation Ltd.)
Based in the United Kingdom. After graduating from a U.S. university, worked
for a design company in London and participated in a great number of
international projects. Became independent and established VO Corporation in
2000. Active in design of automobiles and electronic devices for clients mainly
in Japan, the United States and Europe.
18 19
System diagram: Ni-E focusing stage type
D F C O 1.4J 1.Z0 1AFAN D F C D 0.5 0.80 1AFAN
0.Z 0.4 0.6 0.8 1.0 1.Z 1.4 AA N.A=1.4
1AFAN
D C Oi l
1AFAN
1AFAN
Coarse
Fine
Ex Fine
XY Speed Constant Speed
Z Speed
Coarse
Fine
Ex Fine
Y
Y
X X
FOWER
MlN. MAX.
FOWER
l 0
SFER HlGH FRESSRE MERCRY LAMF FOWER SFFLY
RN TlME
lGNlTlON
FOWER LAMF READY
REFLACE BLB BEFORE THE SFEClFlED TlME
O l
MADE lN 1AFAN
LAMF HORS
FSH ON
FOWER LlGHT
XENON FOWER SFFLY XFS100
0
DFB

A1
6
1
Z
7
7
5 5
4
Z
1
1
1 Double layer is naxinun possible.
Z When used with double layer, "1" connection should be lower
layer and "l" connection should be upper layer. Single "1" layer
is not possible.
J These cubes can only be used with "l" connection.
4 When both "l" and "1" are connected.
5 Cannot be used with double layer status.
6 Cannot be used with double layer status.
7 Should be attached when used with both NiFA and Confocal.
8 CZ can only be used with single layer status.
CHGFlF15/J0
HG Fiber
NZ Transforner
100W
Halogen
Lanp Socket
100W
HMX Lanphouse
CFCL EpiFl
Collector Lens
Quartz EpiFl
Collector Lens
Xenon Fower
Supply 75W
CSHG1
Fower Supply
for HG100W
Xe Lanp
Socket 75W
Xe Lanphouse
HMX4
CFCL EpiFl
Collector Lens
Hg Lanphouse
HMX4B
CFCL EpiFl
Collector Lens
Mercury Lanp
Socket S 100WBL
Hg Lanphouse
HMXJB
CHGFl/HGFlE HG Frecentered
Fiber llluninator lntensilight
CLHGFl
HG Lanp
CHGFlB HG
100WAdapter R
ClA Analyzer Tube for
Firstorder Red Conpensation
ClSA Analyzer
Tube for Sinple Folarization
CER Eyelevel Riser
DES Slider for
Reected lllunination
NlFA Ni Fhotoactivation nit
TlSHAEFl Motorized
Shutter Adapter for
lnverted EpiFL
NlEXWE
Motorized
Excitation
Filter Wheel
NlSHE
Motorized
Shutter
NlSHAEF Motorized
Shutter Adapter
for pright EpiFL
NlSHCS Motorized
Shutter Cable Short
DFB
Excitation Balancer
NlFLEl EFlFluorescence
Attachnent
NlBAWE Motorized
Barrier Filter Wheel
TlA DlC
Analyzer
Cube
DFLE Filter
Cube for Reected
lllunination
DFLD Filter
Cube for Darkeld
lllunination
CFL Epi
Filter Cube
NlFLT6E
Motorized Epi
uorescence
Cube Turret
NlFLT6l
lntelligent Epi
uorescence
Cube Turret
NlFLT6 Epi
uorescence
Cube Turret
NlFA FL/
DlC Analyzer
NlAST Analyzer
Slider Stopper
DSLJ DS
Canera
Control nit
SB Cable
NlCTLA
Control Box A
NlERG Nl
Ergo Controller
NlS1 1oystick for
Motorized Stage
DDF DlC
Rotatable
Folarizer
CTF Folarizer for
Firstorder Red
Conpensation
CSF Folarizer
for Sinple
Folarization
NlLH Frecentered
Lanphouse
NlECB
Connector Box
NlSHADl Motorized
Shutter Adapter for
Dialllunination
NlSHCS Motorized
Shutter Cable Short
NlSHE
Motorized
Shutter
NlNDE
Motorized
ND Filter
ECLIPSE Ni-E
DC DF Darkeld Module
DC FH1 FH Module
DC FHZ FH Module
DC FHJ FH Module
DC DlC N1 DRY DlC Module
DC DlC NZ DRY DlC Module
DC DlC NR DRY DlC Module
DC Z4X Auxiliary Lens for NZ Fosition
NlCALN1 Z4X Auxiliary Lens
for N1 Fosition
NlCD niversal
Condenser Dry
DC DlC Module Oil
DCO DlC
Condenser
Oil
CC Achronat
Swingout
Condenser
1100x
X LWD
Condenser
CC Abbe
Condenser
NA 0.
CC Achronat
Condenser
NA 0.
CC Achronat/
Aplanat
Condenser
Darkeld
Condenser
for X/Y (Dry)
CC Slide
Achronat
Condenser
Z100x
Darkeld
Condenser
for X/Y (Oil)
NlCDE Motorized
niversal Condenser Dry
NlCH
Condenser Holder
NlSSR Substage
for Rotatable/Motorized
Stage
NlECSRRZ Right Handle
Rotatable Ceraniccoated
Stage with Holder
NlSE Motorized XY Stage
CFl W
10x M
CFl W
10x
CCT
Centering
Telescope
CFl 15x
CFl 10x M CFl 10x CFl 1Z.5x
NlRFS
DSC Support
Colunn
NlRFSAB
DSC Adapter B
NlRFSAA
DSC Adapter A
NlRFZ
DSC Zooning Fort for
Quadrocular Tube
NlRFZE Motorized
DSC Zooning Fort for
Quadrocular Tube
NlTT Quadrocular
Tilting Tube
NlTTE Motorized
Quadrocular Tilting Tube
CTAQ Tube
Adapter for
Quadrocular Tube
DlC Slider
DLF
Lanbda Flate
CAS Analyzer Slider for
Firstorder Red Conpensation
DSA Analyzer Slider
for Sinple Folarization
DDA Analyzer
Slider for DlC CFl60
Ob|ective Lens
NlN7E
Motorized
Septuple
Nosepiece
NlND6E
Motorized DlC
Sextuple Nosepiece
NlN7l lntelligent
Septuple Nosepiece
NlND6l lntelligent
DlC Sextuple Nosepiece
DND6 DlC
Sextuple Nosepiece
CNA Sextuple Nosepiece
with Analyzer Slot
LDlC DlC Slider
LN5 niversal
Quintuple
Nosepiece ESD
LNBD5 BD
Quintuple
Nosepiece ESD
LDlHC DlC Slider HC
CFl60
Ob|ective Lens
CFl L Flan Fluor
EFl Ob|ective Lens
CFl L Flan Fluor BD
Ob|ective Lens
NlECAM Contact Arn NlSAM Standard Arn
NlDRS
DSC Rotation Stopper
YTv 0.55
Tv Tube
YTv Tv
Tube
vT
Fhoto Adapter
Cnount
Tv Adapter
vM4x
Cnount
Tv Adapter
vMZ.5x
Relay
Lens
1x
Cnount
Tv Adapter
Tv Zoon
Lens
Relay
Lens
1x
Cnount
Adapter
0.55x
Cnount
Adapter
0.7x
Cnount
Tv Adapter A
Cnount
Tv Adapter
0.J5x,
0.45x,
0.6x
Cnount
Zooning
Adapter
ENGnount
Zooning
Adapter
ENGnount
Tv Adapter
ENGnount
Tv Adapter
0.45x, 0.6x
Fro|ection
Lens
FLl Zx,
FLl Z.5x,
FLl 4x,
FLl 5x
C-mount Camera ENG-mount Camera FX-III Series
Photomicrographic
System
CTEFZ DSC Fort
for Ergononic
Binocular Tube
CTEZ
Ergononic
Binocular Tube
CTT
Trinocular
Tube T
CTF
Trinocular
Tube F
CTB
Binocular
Tube
8 CT TC
Trinocular
Tube
CZ
Adapter
A1
Adapter
CZ
Achronat
Swingout
Condenser
Z100x
CN Sextuple
Nosepiece
L Nosepiece Adapter
J

@
@
@
@
@
@

'

'
' '
'

@
@
@ @
@

'
'
'

'

@
@

'
'

@
@
@

'
@
@
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@
@ @

Z
J
4
Z
J
4

@ @
@
@
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@
20 21
1AFAN
1AFAN
Coarse
Fine
Ex Fine
XY Speed Constant Speed
Z Speed
Coarse
Fine
Ex Fine
Y
Y
X X
DFB

N 1 Dr y
1AFAN
SB Cable
A1
1 Z
5
5
Z
1
1
1 Double layer is naxinun possible.
Z When used with double layer, "1" connection should be lower
layer and "l" connection should be upper layer. Single "1" layer
is not possible.
J These cubes can only be used with "l" connection.
4 Cannot be used with double layer status.
5 Should be attached when used with both NiFA and Confocal.
CHGFlF15/J0
HG Fiber
CHGFl/HGFlE HG Frecentered
Fiber llluninator lntensilight
CLHGFl
HG Lanp
CHGFlB HG
100WAdapter R
DES Slider for
Reected lllunination
NlFA Ni Fhotoactivation nit
TlSHAEFl Motorized
Shutter Adapter for
lnverted EpiFL
NlEXWE
Motorized
Excitation
Filter Wheel
NlSHE
Motorized
Shutter
NlSHAEF Motorized
Shutter Adapter
for pright EpiFL
NlSHCS Motorized
Shutter Cable Short
DFB
Excitation Balancer
NlFLEl EFlFluorescence
Attachnent
NlBAWE Motorized
Barrier Filter Wheel
TlA DlC
Analyzer
Cube
A1FN1lR
Analyzer
Block lR
DFLE Filter
Cube for Reected
lllunination
DFLD Filter
Cube for Darkeld
lllunination
CFL Epi
Filter Cube
NlFLT6E
Motorized Epi
uorescence
Cube Turret
NlFLT6l
lntelligent Epi
uorescence
Cube Turret
NlFLT6 Epi
uorescence
Cube Turret
NlFA FL/
DlC Analyzer
NlAST Analyzer
Slider Stopper
DSLJ DS
Canera
Control nit
NlCTLA
Control Box A
NlERG Nl
Ergo Controller
NlS1 1oystick for
Motorized Stage
NlECB
Connector Box
NlSHADl Motorized
Shutter Adapter for
Dialllunination
NlSHCS Motorized
Shutter Cable Short
NlRFS
DSC Support
Colunn
NlRFSAB
DSC Adapter B
NlRFSAA
DSC Adapter A
CTAQ Tube
Adapter for
Quadrocular Tube
NlECAM Contact Arn NlSAM Standard Arn
NlDRS
DSC Rotation Stopper
YTv
0.55
Tv Tube
YTv
Tv Tube
vT
Fhoto Adapter
Cnount
Tv Adapter
vM4x
Cnount
Tv Adapter
vMZ.5x
Relay
Lens
1x
Cnount
Tv Adapter
Tv Zoon
Lens
Relay
Lens
1x
Cnount
Adapter
0.55x
Cnount
Adapter
0.7x Cnount
Tv Adapter A
Cnount
Tv Adapter
0.J5x,
0.45x,
0.6x
Cnount
Zooning
Adapter
ENGnount
Zooning
Adapter
ENGnount
Tv Adapter
ENGnount
Tv Adapter
0.45x, 0.6x
Fro|ection
Lens
FLl Zx,
FLl Z.5x,
FLl 4x,
FLl 5x
C-mount Camera ENG-mount Camera FX-III Series
Photomicrographic
System
CTEFZ DSC Fort
for Ergononic
Binocular Tube
4 CTT
Trinocular
Tube T
4 CTF
Trinocular
Tube F
CTB
Binocular
Tube
CZ
Adapter
A1
Adapter
CZ
NlRFZ
DSC Zooning Fort for
Quadrocular Tube
NlRFZE Motorized
DSC Zooning Fort for
Quadrocular Tube
NlTT Quadrocular
Tilting Tube
NlTTE Motorized
Quadrocular Tilting Tube
4
CFl W
10x M
CFl W
10x
CCT
Centering
Telescope
CFl 15x
CFl 10x M CFl 10x CFl 1Z.5x
CTEZ
Ergononic
Binocular Tube
NlSSF Substage
for Focusing
Nosepiece
DC DlC NZ
DRY DlC Module
DC DlC N1
DRY DlC Module
FNFT4X 4X
Support Lens
FNC LWD
Condenser
NlSHD
Dish Holder
NlSE Motorized
XY Stage
NlSA Motorized
XY Stage Adapter
FNJFSZ FN1
Standard Stage
NlJFSA Adapter for
FN1 Standard Stage
CFl60
Ob|ective Lens
DlC Slider CFl75
Ob|ective Lens
Ob|ective
Slider
Ob|ective
Holder
DlC Slider
NlFNA Focusing
Nosepiece Sub Arn
NlAMSL Arn Spacer 74nn
NlAMSS Arn Spacer 4nn
NlFT Folarizer
Turret
NlLH Frecentered
Lanphouse
NllRLH Frecentered lR
Lanphouse
NlFlA Fiber
lllunination Adapter
NlSHE
Motorized
Shutter
NlNDE
Motorized
ND Filter
ECLIPSE Ni-E
FNlRF lR Floarizer
FNF Folarizer

Fiber llluninator
J

@
@
@
@
@

'

'
'
'

'

@ @

@
@
@

'
@
@
@
@
@
@ @
@
@
System diagram: Ni-E focusing nosepiece type
22 23
System diagram: Ni-U
1AFAN
1AFAN
7
1
1
1
8
Z
Z
5
5
4
8
6 6
1 Double layer is naxinun possible.
pper layer accepts only NlFLT6 when double layer is used.
Z When used with double layer, "1" connection should be lower layer and
"l" connection should be upper layer. Single "1" layer is not possible.
J These cubes can only be used with "l" connection.
4 When both "l" and "1" are connected.
5 DSLJ can be connected directly when NiCTLB is not used.
6 Cannot be used with double layer status.
7 Cannot be used with double layer status.
8 Should be attached when used with both NiFA and Confocal.
CZ can only be used with single layer status.
NlFLT6E
Motorized Epi
uorescence Cube Turret
NlFLT6l
lntelligent
Epiuorescence
Cube Turret
NlFLT6
Epiuorescence
Cube Turret
DC DF Darkeld Module
DC FH1 FH Module
DC FHZ FH Module
DC FHJ FH Module
DC DlC N1 DRY DlC Module
DC DlC NZ DRY DlC Module
DC DlC NR DRY DlC Module
DC Z4X Auxiliary Lens
for NZ Fosition
NlCALN1 Z4X Auxiliary Lens
for N1 Fosition
NlCD niversal
Condenser Dry
DC DlC
Module Oil
DCO DlC
Condenser
Oil
CC Achronat
Swingout
Condenser
1100x
X LWD
Condenser
CC Abbe
Condenser
NA 0.
CC Achronat
Condenser
NA 0.
CC Achronat/
Aplanat
Condenser
Darkeld
Condenser
for X/Y (Dry)
CC Slide
Achronat
Condenser
Z100x
Darkeld
Condenser
for X/Y (Oil)
CC Fhase
Contrast Turret
Condenser
CFl W
10x M
CFl W
10x
CCT
Centering
Telescope
CFl 15x
CFl 10x M CFl 10x CFl 1Z.5x
NlRFS
DSC Support
Colunn
NlRFSAB
DSC Adapter B
NlRFSAA
DSC Adapter A
NlRFZ
DSC Zooning Fort for
Quadrocular Tube
NlTT Quadrocular
Tilting Tube
CTAQ Tube
Adapter for
Quadrocular Tube
NlCAM Contact Arn NlSAM Standard Arn
NlDRS
DSC Rotation Stopper
YTv
0.55
Tv Tube
YTv
Tv Tube
vT
Fhoto Adapter
Cnount
Tv Adapter
vM4x
Cnount
Tv Adapter
vMZ.5x
Relay
Lens
1x
Cnount
Tv Adapter
Tv Zoon
Lens
Relay
Lens
1x
Cnount
Adapter
0.55x
Cnount
Adapter
0.7x
Cnount
Tv Adapter A
Cnount
Tv Adapter
0.J5x,
0.45x,
0.6x
Cnount
Zooning
Adapter
ENGnount
Zooning
Adapter
ENGnount
Tv Adapter
ENGnount
Tv Adapter
0.45x, 0.6x
Fro|ection
Lens
FLl Zx,
FLl Z.5x,
FLl 4x,
FLl 5x
C-mount Camera ENG-mount Camera FX-III Series
Photomicrographic
System
CTEFZ DSC Fort
for Ergononic
Binocular Tube
CTEZ
Ergononic
Binocular Tube
CTT
Trinocular
Tube T
CTF
Trinocular
Tube F
CTB
Binocular
Tube
CT TC
Trinocular
Tube
CZ
Adapter
CZ
NZ Transforner
100W
Halogen Lanp
Socket 100W
HMX Lanphouse
CFCL EpiFl
Collector Lens
Quartz EpiFl
Collector Lens
Xenon
Fower Supply
75W
CSHG1
Fower Supply
for HG100W
Xe Lanp
Socket 75W
Xe Lanphouse
HMX4
CFCL EpiFl
Collector Lens
Hg Lanphouse
HMX4B
CFCL EpiFl
Collector Lens
Mercury Lanp
Socket S 100WBL
Hg Lanphouse
HMXJB
CHGFl/HGFlE HG
Frecentered Fiber
llluninator lntensilight
CLHGFl HG Lanp
CHGFlB HG 100W
Adapter R
CHGFlF15/J0
HG Fiber
DSLJ DS
Canera Control nit
NlSRCF
Sinple Renote
Control Fad NlCTLB
Control Box B
CCTC Canera
Trigger Cable
J/LJ
CCTC Canera
Trigger Cable
J/LJ
Tl100WRC 100W
Lanphouse
Renote Cable
DDF DlC
Rotatable
Folarizer
CTF Folarizer
for Firstorder
Red Conpensation
CSF Folarizer
for Sinple
Folarization
NlLH Frecentered
Lanphouse
NlSHADl Motorized
Shutter Adapter for
Dialllunination
NlSHCL Motorized
Shutter Cable Long
NlSHE
Motorized Shutter
ECLIPSE Ni-U
NlCH
Condenser Holder
NlSSR Substage
for Rotatable/
Motorized Stage
NlCSRRZ
Right Handle Rotatable
Ceraniccoated Stage
with Holder
CH1L Specinen
Holder 1L CHZL Specinen
Holder ZL
NlSS Substage
CCSR1S Right
Handle Ceraniccoated
Stage with 1S Holder
CSRZS
Right Handle Stage
with ZS Holder
CCSR Right
Handle Ceraniccoated
Stage
DES Slider for
Reected lllunination
NlFA
Ni Fhotoactivation nit
NlSHE
Motorized Shutter
NlSHAEF
Motorized
Shutter Adapter
for pright EpiFL
NlSHCL
Motorized Shutter
Cable Long
DFB Excitation
Balancer
NlFLEl EFlFluorescence
Attachnent
NlFA FL/DlC
Analyzer
NlAST Analyzer
Slider Stopper
CER Eyelevel Riser
ClSA Analyzer Tube for
Sinple Folarization
ClA Analyzer Tube for
Firstorder Red Conpensation
YTHFL LED
Fointer nit
for Teaching Head
YlM Magnication Module
YTHF Teaching nit Face to Face
YTHS Teaching nit Side by Side nit B
THACZ AC Adapter
YTHM Main
Teaching nit
YTHR Teaching nit Side by Side A
YTHSF Support
for Side by Side
Achronat
Swingout
Condenser
Z100x
DlC Slider
DLF
Lanbda Flate
CAS Analyzer Slider for
Firstorder Red Conpensation
DSA Analyzer Slider
for Sinple Folarization
DDA Analyzer
Slider for DlC CFl60
Ob|ective Lens
LDlC DlC Slider
LN5 niversal
Quintuple
Nosepiece ESD
LNBD5 BD
Quintuple
Nosepiece ESD
LDlHC DlC Slider HC
CFl60
Ob|ective Lens
L Nosepiece Adapter
CFl L Flan Fluor
EFl Ob|ective Lens
CFl L Flan Fluor BD
Ob|ective Lens
J
TlA DlC
Analyzer
Cube
DFLE Filter
Cube for Reected
lllunination
DFLD Filter
Cube for Darkeld
lllunination
CFL Epi
Filter Cube @
@
NlN7E
Motorized
Septuple
Nosepiece
NlND6E
Motorized DlC
Sextuple Nosepiece
NlN7l lntelligent
Septuple Nosepiece
NlND6l lntelligent
DlC Sextuple Nosepiece
DND6 DlC
Sextuple Nosepiece
CNA Sextuple Nosepiece
with Analyzer Slot
CN Sextuple
Nosepiece
@
@
@
@
@
@

'

@
@
@ @
@

'

'

'

'

@
@
@
@
@

@
@
@

@
Z
J
4
Z
J
4 @ @

@
@ @

@
@ @

@
Specifcations
24 25
Ni-E
Ni-U
Focusing stage type Focusing nosepiece type
Main body
Optical system CFI60 infnity optical system CFI60 and CFI75 infnity optical systems CFI60 infnity optical system
Focusing
(stroke from focus
point)
Via motorized stage Up/Down movement
(Up 2 mm/Down 13 mm)
Via motorized nosepiece Up/Down movement
(Up 13 mm/Down 2 mm)
Via manual stage Up/Down movement
(Up 3 mm/Down 26 mm)
Built-in linear encoder, Resolution: 0.025 m
Motorized escape and refocus mechanism
Coaxial Coarse/Fine focusing
Illumination
Halogen lamp (12V100W)
NI-ND-E Motorized ND Filter (option)
Halogen lamp (12V100W)
Built-in fy-eye lens
Built-in NCB11, ND8, ND32 flters (detachable, one additional flter mountable) and diffuser (non-detachable)
Controls
Transmitted light On/Off switch, Intensity control dial with preset function
Image capture button
Built-in motorized control switches
Stand-alone control unit DS-L3
NI-ERG NI Ergo Controller (option)
Stand-alone control unit DS-L3 (option)
NI-SRCP Simple Remote Control Pad
(option)
Power supply unit External power supply NI-CTLA Control Box A for all confgurations
Built-in for halogen lamp
NI-CTLB Control Box B is necessary
when Motorized/Intelligent options are
combined.
Eyepieces (F.O.V. mm)
CFI 10x (22) CFI 10xM Photomask (22) CFI 12.5x (16)
CFI 15x (14.5) CFI UW10x (25) CFI UW10xM Photomask (25)
Tubes
F.O.V. 22 mm
(Eyepiece/Port)
C-TB Binocular Tube
C-TE2 Ergonomic Binocular Tube (100/0, 50/50 with C-TEP2 DSC Port)
Inclination angle: 10-30 degree, Extension up to 40 mm
F.O.V. 25 mm
*1

(Eyepiece/Port)
C-TF Trinocular Tube F (100/0, 0/100)
C-TT Trinocular Tube T (100/0, 20/80, 0/100)
C-TT-C Trinocular Tube (100/0, 0/100, for confocal)
*2

NI-TT Quadrocular Tilting Tube (Eyepiece/Upper port/Rear port: 100/0/0, 0/100/0, 0/0/100)
Inclination angle: 15-35 degree
NI-TT-E Motorized Quadrocular Tilting Tube
(Eyepiece/Upper port/Rear port: 100/0/0, 0/100/0, 0/0/100)
Inclination angle: 15-35 degree

Arms
NI-SAM Standard Arm
NIE-CAM Contact Arm (for Motorized/Intelligent options)
NIU-CAM Contact Arm (for
Motorized/Intelligent options)
Nosepieces
Motorized
NI-N7-E Motorized Septuple Nosepiece
NI-ND6-E Motorized Sextuple DIC Nosepiece

NI-N7-E Motorized Septuple
Nosepiece
NI-ND6-E Motorized Sextuple DIC
Nosepiece
Intelligent
NI-N7-I Intelligent Septuple Nosepiece
NI-ND6-I Intelligent Sextuple DIC Nosepiece

NI-N7-I Intelligent Septuple Nosepiece
NI-ND6-I Intelligent Sextuple DIC
Nosepiece
Manual
D-ND6 Sextuple DIC Nosepiece
C-N Sextuple Nosepiece
C-NA Sextuple Nosepiece with Analyzer Slot
L-NU5 Universal Quintuple Nosepiece ESD
L-NBD5 BD Quintuple Nosepiece ESD
FN-S2N Sliding Nosepiece
(for CFI60 objectives)
Changeover 2 objectives, DIC slider insertable
FN-MN-H Single Objective Holder
(for CFI75 objective)
DIC slider insertable
D-ND6 Sextuple DIC Nosepiece
C-N Sextuple Nosepiece
C-NA Sextuple Nosepiece with
Analyzer Slot
L-NU5 Universal Quintuple Nosepiece
ESD
L-NBD5 BD Quintuple Nosepiece ESD
Stages
NIE-CSRR2 Right Handle Rotatable Ceramic-
coated Stage with 2S Holder
Cross travel 78(X) x 54(Y) mm
Handle height and torque adjustable
FN-3PS2 FN1 Standard Stage
Cross travel 30(X) x 30(Y) mm
C-SR2S Right Handle Stage with 2S
Holder
C-CSR1S Right Handle Ceramic-coated
Stage with 1S Holder
C-CSR Right Handle Ceramic-coated
Stage
NIU-CSRR2 Right Handle Rotatable
Ceramic-coated Stage with 2S Holder
Cross travel 78(X) x 54(Y) mm
Handle height and torque adjustable
NI-S-E Motorized XY Stage
Resolution: 0.1 m
NI-SH-D Dish Holder (option)
Substages
NI-SSR Substage
(for Motorized Universal Condenser and
Rotatable/Motorized stages)
NI-SSF Substage for Focusing Nosepiece
(for LWD condenser and FN1 Standard/Motorized
stages)
NI-SSR Substage (for Rotatable stage)
NI-SS Substage (for Non-rotatable
stages)
Ni-E
Ni-U
Focusing stage type Focusing nosepiece type
Condensers
Motorized
NI-CUD-E Motorized Universal Condenser Dry
For DIC, phase contrast, darkfeld
observations
Attached on NI-SSR Substage

Manual
NI-CUD Universal Condenser Dry
C-C Abbe Condenser NA 0.9
C-C Achromat Condenser NA 0.9
Darkfeld Condenser (Oil)
Darkfeld Condenser (Dry)
C-C Achromat/Aplanat Condenser NA 1.4
C-C Slide Achromat Condenser 2-100x
C-C Achromat Swing-out Condenser 1-100x
Achromat Swing-out Condenser 2-100x
X LWD Condenser
D-CUO DIC Condenser Oil
FN-C LWD Condenser NA 0.78
(for DIC and oblique light illumination)
NI-CUD Universal Condenser Dry
C-C Abbe Condenser NA 0.9
C-C Achromat Condenser NA 0.9
Darkfeld Condenser (Oil)
Darkfeld Condenser (Dry)
C-C Achromat/Aplanat Condenser
NA 1.4
C-C Slide Achromat Condenser 2-100x
C-C Achromat Swing-out Condenser
1-100x
C-C Phase Contrast Turret Condenser
*3

Achromat Swing-out Condenser 2-100x
X LWD Condenser
D-CUO DIC Condenser Oil
Epi-
fuorescence
illuminator
Filter cube turret
6 flter cubes mountable, High S/N noise terminator mechanism for all turrets
NI-FLT6-E Motorized Epi-fuorescence Cube Turret
Motorized shutter, Status check function
*4

NI-FLT6-I Intelligent Epi-fuorescence Cube Turret
Manual shutter, Status check function
*4

NI-FLT6 Epi-fuorescence Cube Turret
Manual shutter
Light distribution
device
NI-FLEI Epi-fuorescence Attachment
Aperture diaphragm and feld diaphragm (Centerable/Detachable), ND flters (ND4, ND8, ND16)
NI-PAU Ni Photoactivation Unit (405 nm to 650 nm lasers)
Option
NI-BAW-E Motorized Barrier Filter Wheel
7 flters mountable, 0.2 sec between adjacent positions
NI-EXW-E Motorized Excitation Filter Wheel
8 flters mountable, 0.15 sec between adjacent positions
NI-SH-E Motorized Shutter
0.012 sec between open and close state
NI-SH-E Motorized Shutter
0.012 sec between open and close state
Epi-illumination light source
C-HGFI/HGFIE HG Precentered Fiber Illuminator Intensilight (130W)
Hg Lamphouse and Power Supply (100W)
*2

Xe Lamphouse and Power Supply (75W)
*2

Halogen Lamphouse and Transformer (100W)
*2
Power consumption
211W
(with max. halogen lamp intensity
and full motorized options)
96W
(with max. halogen lamp intensity
and full motorized options)
Main body: 133W (with max. halogen
lamp intensity)
Control Box B: 29W (with full motorized
options)
Weight (approx.)
29 kg
(Epi-fuorescent confguration with motorized
quadrocular tilting tube)
42 kg
(Photoactivation confguration with motorized
quadrocular tilting tube)
20 kg
(Brightfeld confguration with ergonomic
binocular tube)
*1 When used with a double layer, such as with layered epi-fuorescence cube turrets, F.O.V. is 22.
*2 Cannot be used with the focusing nosepiece type.
*3 Can only be mounted on the NI-SS Substage.
*4 Status check function: Status of Filter/Nosepiece etc. can be recorded with captured images and/or displayed on the controller monitor.
Dimensional diagram
130
6
0
2
0
5
437
6
0
4
320
3
8
9

A.S
JAPAN FN-S2N
0
Complies with FDA performance standard for laser products except for deviations pursuant to Laser Notice No.50,dated June 24,2007.
IEC/EN60825-1:2007 Total Power 500mWMAX. CW400700nm
320 383
2
0
5
3
4
0
6
0
4
8
8
130
A

320 383
2
0
5
3
4
0
6
0
130
5
0
1
26 27
Ni-E (for use with focusing nosepiece)
Confgured with an Ni photoactivation unit, two-tiered
motorized epi-fuorescence cube turret and motorized
quadrocular tilting tube
Ni-U
Confgured with a motorized epi-fuorescence cube turret
and quadrocular tilting tube
Ni-U
Confgured with an ergonomic binocular tube and DSC port
Unit: mm
En
Printed in Japan (1111-10)T Code No. 2CE-MQVH-3
Specifications and equipment are subject to change without any notice or obligation on the part
of the manufacturer. November 2011 2011 NIKON CORPORATION
Company names and product names appearing in this brochure are their registered trademarks or trademarks.
N.B. Export of the products* in this brochure is controlled under the Japanese Foreign Exchange and Foreign Trade Law.
Appropriate export procedure shall be required in case of export from Japan.
*Products: Hardware and its technical information (including software)
Monitor images are simulated.
WARNING
TO ENSURE CORRECT USAGE, READ THE CORRESPONDING
MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT.
This brochure is printed on recycled paper made from 40% used material.
NIKON CORPORATION
Shin-Yurakucho Bldg., 12-1, Yurakucho 1-chome, Chiyoda-ku, Tokyo 100-8331, Japan
phone: +81-3-3216-2375 fax: +81-3-3216-2385
http://www.nikon.com/instruments/
NIKON INSTRUMENTS INC.
1300 Walt Whitman Road, Melville, N.Y. 11747-3064, U.S.A.
phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A. only)
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phone: +31-20-44-96-300 fax: +31-20-44-96-298
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NIKON INSTRUMENTS (SHANGHAI) CO., LTD.
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UNITED KINGDOM phone: +44-208-247-1717 fax: +44-208-541-4584
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AUSTRIA phone: +43-1-972-6111-00 fax: +43-1-972-6111-40
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BELGIUM phone: +32-2-705-56-65 fax: +32-2-726-66-45