Protection to species

NATURE

DENTAL BIOFILM
are Soft, adherent, structural deposits Are found in

formation

Pellicle formation

Nutrient uptake facilitation

Bacterial blooms Palisading appearance in electron microscope

Characterized by

Bacterial adhesion

communication

Composed of

Mouth or wet environment includes

How?

Doubling times are rapid initally Contain areas of high and low bacterial biomass interlaced with aqueous channel

Sequential adsorption

Signalling mollecules

Genetic information exchange

Intercellular martix

microorganisms

Extracellular polysaccharide Dentition and removable appliances

Detachment

Composed of

Organic constituents

Inorganic constituents

Prevent attack by harmful agents

Function

Maintain integrity Prevent desiccation Acts as buffer; assist in retention of extracellular enzymes

Dental Biofilm Formation

Sequential Adsorption

Pellicle formation

Bacterial Adhesion

shift to an increase in gram - anaerobic bacteria

Detachment

Conditions biofilm formation

facultative gram + cocci

bacteria with protein adhesins

multiplication of adhering bacteria

growth of ECM

virulence factors

migration/translocation

Pellicle forms 30 minutes after brushing

streptococcus sp.

coaggregation

trigger inflammation

oxygen gradient

serves as protective barrier against desiccation

actinomyces sp.

changes tooth energy