LOYOLA COLLEGE (AUTONOMOUS), CHENNAI 600 034

B.Sc. DEGREE EXAMINATION ADVAN. ZOOLOGY & BIO-TECH. & CHEMISTRY FOURTH SEMESTER APRIL 2010 PB 4210 / 4206 / 4202 - MICROBIAL BIOTECHNOLOGY Date & Time: 19/04/2010 / 9:00 - 12:00 Dept. No. Max. : 100 Marks

PART A (20 marks) Answer All questions I Choose the Correct Answer ( 5 x 1 = 5 marks) 01. Analytical technique used for the separation of DNA fragments is .. a. PAGE b. SDS-PAGE c. AGE d. PCR 02. . is the byproduct of dairy industry which is a good carbon source in fermentation industry. a. Butter milk b. Cheese c. Whey d. Yoghurt 03. . is the starting material for production of steroids. a. Diosgenin b. Streptomycin c. Cephalosporin d. Penicillin 04. Peroxidase enzyme is produced from.. a. soy bean b. horse radish c. papaya d. fig 05. Which of the following microorganism produces hexopolysaccharide? a. Xanthomonas campestris b. Pseudomonas elodea c. Leuconostoc mesentenoides d. Acinetobactor sps. II State whether the following statements are True or False: (5 x 1 = 5 marks) 06. Intracellular metabolites are located outside the cells. 07. Photobioreacter is used for the production of secondary metabolites. 08. Commercial production of enzymes is mostly from microbes. 09. The most commonly used food preservative is glutamic acid. 10. BGA in aquatic fern fixes atmospheric nitrogen. III. Complete the following 11. Taq DNA polymerase enzyme is extracted from ___________ 12. Bioreactor is used for the culturing of ________ 13. The amount of Vitamin B12 produced by Propionibacterium sps. is _____ mg/L 14. Commercially available glutamic acid is called as__________ 15. _________ is an intra cellular, carbon and energy rich biodegradable polymer. IV. Answer all the questions, each in about 50 words 16. Define transgenic microbe. 17. What is inoculum build up? 18. Distinguish between surface and submerged fermentation 19. What is artificial sweetener? 20. Define yoghurt ( 5 x 1 = 5 marks) ( 5 x 1 = 5 marks)

PART B

( 5 x 7 = 35 marks)

Answer the following, each within 350 words only. Draw diagrams and flowcharts wherever necessary. 21a. Define Biotechnology. Write notes on restriction enzymes. Or b. Explain the method of DNA amplification.

22a. Explain the types of microbial growth in fermentation. Or b. Explain the different parts of a fermentor along with their functions. 23a. Briefly explain the commercial production of citric acid. Or b. Explain the production methodology of protease. 24a. Give an account of biosynthesis of Penicillin Or b. Explain the production methodology of Streptomycin. 25a. What is single cell protein? Describe the methodology of mass cultivation of Spirulina. Or b. Explain the steps involved in cheese production. PART C (3x 15 = 45 marks)

Answer any three of the following, each within 1500 words only. Draw diagrams and flowcharts wherever necessary. 26. Explain the steps involved in the production of recombinant strain. 27. Describe the process of fermentation technology. Add a note on carbon and nitrogen substrate used in fermentation industry. 28. What is biotransformation? Explain the process of biotransformation of steroids. 29. Mention any one microbial source and write about the applications of gultamic acid, citric acid and acetic acid. 30. Define Biosensor. Explain the working principle, types and applications of biosensor. **************