Beruflich Dokumente
Kultur Dokumente
RNA Pol I
RNA Pol II
Nucleoli
Nucleoplasm Nucleoplasm
Insensitive
Very sensitive Moderately sensitive
PROMOTER
Promoters usually have 4 Elements: 1. TATA Box (25) 2. Initiator Element (Inr) 3. Downstream Core Promoter Element 4. CAAT box and some contain GC Box (-40 s/d -150)
5 CAP
From GTP
Initiating nucleotide
SPLICING
Mechanisms Transesterification
Translational control
Structure of eukaryotic and prokaryotic mRNAs:
Pemanjangan
Elongation Factor (EF)-Tu Enzim peptidil transferase EF-G
Terminasi
Kodon terminasi (UAA, UGA, UAG) eRF 1 dan 3
rRNAs are believed to play a catalytic role in protein synthesis. After removal of 95% of the ribosomal proteins, the 60S subunit can catalyze formation of peptide bonds. Ribosomal proteins are now believed to help fold the rRNAs properly and to position the tRNAs.
The first step is the recognition of the 5 cap by eIF4F, which consists of three proteins, eIF4E, eIF4G and eIF4A.
Elongation
Ribosome selects aminoacylated tRNA
eEF1a GTP
P A
Termination Translation is terminated at one of three stop codons (UAA, UAG & UGA).
Termination codon at the A site is recognized by the release factor instead of a tRNA
The release factor binds the termination codon The peptide chain is then released followed by dissociation of the tRNA and the ribosome