Code No: RR322302

Set No. 1

III B.Tech II Semester Supplementary Examinations, Aug/Sep 2006 ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. What is the principle of Fluorescence Microscopy? 2. List out the prerequisites for viewing any biological specimen under TEM. [16] [16]

3. What are Nucleotides? At what wavelength can they be absorbed and why? [16] 4. What is the principle behind Electrophoresis? 5. Write short notes on (a) Gel ltration chromatography (b) Anity chromatography 6. How is PCR used to sequence DNA? 7. Dierentiate between single and double Electroimmunodiusion. 8. Discuss product formation kinetics in cell culture. [8+8] [16] [16] [16] [16]

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Code No: RR322302

Set No. 2

III B.Tech II Semester Supplementary Examinations, Aug/Sep 2006 ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. What is the principle of Fluorescence Microscopy? 2. What is the dierence between a TEM and a SEM? [16] [16]

3. What are the general biochemical tests performed to detect the presence of any carbohydrates? [16] 4. What is PAGE? 5. Write short notes on (a) Gel ltration chromatography (b) Anity chromatography 6. How is PCR used to sequence DNA? 7. Why is RIA considered as a competitive binding Immunoassay? 8. What is meant by ow injection analysis? [8+8] [16] [16] [16] [16]

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Code No: RR322302

Set No. 3

III B.Tech II Semester Supplementary Examinations, Aug/Sep 2006 ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. Describe a Fluorescent Microscope. 2. Write short on (a) TEM specimen preparation step - Fixation (b) TEM specimen preparation step - Inltration with embedded medium [8+8] 3. (a) Distinguish between Nucleotides and Nucleosides (b) Deoxyribose and ribose 4. What are the factors aecting electrophoretic mobility of any molecule? 5. Write about Dot slot blots. 6. Explain antisera production in detail. 7. What is the dierence between direct and indirect ELISA techniques? 8. What is meant by ow injection analysis? [8+8] [16] [16] [16] [16] [16] [16]

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Code No: RR322302

Set No. 4

III B.Tech II Semester Supplementary Examinations, Aug/Sep 2006 ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. What is Bright Field Microscopy? 2. Discuss image formation in scanning electron microscope. 3. Write about the classication and biological roles of lipids. 4. 2D Electrophoresis is a combination of which two techniques? 5. Explain Northern Blotting. 6. Explain antisera production in detail. 7. What is the principle behind Immunoelectrophoresis? 8. Explain the terms (a) Dissolved Oxygen (DO) (b) Dissolved Carbon dioxide (DCO2 ) (c) Oxygen transfer rate (OTR) (d) Oxygen uptake rate (OUR) [4+4+4+4] [16] [16] [16] [16] [16] [16] [16]

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