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HT-1080 RAW 264.7 K-562 PC-3
15
10
5
0
Reference Reagent
X-tremeGENE HP DNA Transfection Reagent
Competitor LTX P
5
X-tremeGENE 9 DNA Transfection Reagent
High Cell Survival
Due to its low cytotoxicity, minimal optimization, and high
transfection efficiency in commonly used cell lines, such as
HeLa, CHO-K1, NIH-3T3 and COS-7, X-tremeGENE 9 Reagent is
perfectly suited for applications in all fields of cellular analysis,
even with serum.
Obtain maximum cell viability
after transfection.
Generate physiologically relevant data
using a reagent with low cytotoxic effects.
Save time and effort,
and avoid time-consuming optimization.
Figure 2. High cell survival using X-tremeGENE 9 DNA Transfection
Reagent. HeLa cells were transfected. Reagent cytotoxicity was measured using
the Roche LDH Cytotoxicity Detection Kit. The ratios of l reagent to
l DNA used, are indicated. X-tremeGENE 9 Reagent showed lower cytotoxicity,
compared to the competitor reagents.
Competitor
LTX P Reagent
%
T
o
x
i
c
i
t
y
2:1 3:1 2:1 3:1 1.5:1:1 3:1:1
40
30
20
10
0
X-tremeGENE 9 DNA
Transfection Reagent
Competitor
L2K Reagent
6
Note: Above data were produced using either a GFP-encoding
pcDNA3.1 plasmid or a Luciferase-encoding pCI plasmid, both with
the cytomegalovirus (CMV) promoter. These recommendations are
guidelines based on experimental findings.
The optimal reagent: DNA ratio must be determined empirically.
Selection Table for X-tremeGENE DNA Transfection Reagents
X-tremeGENE HP
DNA Transfection
Reagent
X-tremeGENE 9
DNA Transfection
Reagent
Transfection of DNA +++ +++
Efciency with standard cell lines
(e.g., COS-7, HEK-293, HeLa,
NIH 3T3, CHO-K1)
+++ +++
Efciency with
difcult-to-transfect cells
(e.g., HT 29, HCT 116, K-562)
+++ +
Transfection of primary cells ++(+) not recommended;
exceptions are
possible
Gentleness of the reagent ++(+) +++
Ease of use
(minimal optimization)
+++ +++
Cytotoxicity after transfection Very low Very low to
exceptionally low
Storage -15 to -25 C +2 to +8 C
Product Catalog Number Pack Size
X-tremeGENE HP
DNA Transfection
Reagent
06 366 244 001
06 366 236 001
06 366 546 001
0.4 ml
1.0 ml
5 x 1 ml
X-tremeGENE 9
DNA Transfection
Reagent
06 365 779 001
06 365 787 001
06 365 809 001
0.4 ml
1.0 ml
5 x 1 ml
Ordering Information
7 Commonly Used Cell Types
CHO-K1 Cells
Plate CHO-K1 cells at a density of 1.5 10
4
cells/well.
Plate cells in a volume of 100 l complete growth medium
per well in a 96-well plate 18 24 hours before transfection
(65 75% confluency). Incubate cell cultures overnight.
Allow X-tremeGENE 9 DNA Transfection Reagent, DNA and
diluent (Opti-MEM
The next day refresh the medium with complete growth medium.